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Protein

SUMO-conjugating enzyme UBC9

Gene

Ube2i

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin-like proteins SUMO1, SUMO2 and SUMO3 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Essential for nuclear architecture, chromosome segregation and embryonic viability. Necessary for sumoylation of FOXL2 and KAT5 (By similarity). Sumoylates p53/TP53 at 'Lys-386'.By similarity3 Publications

Catalytic activityi

SUMOyl-[E2 SUMO-conjugating enzyme]-L-cysteine + [acceptor-protein]-L-lysine = [E2 SUMO-conjugating enzyme]-L-cysteine + N6-SUMOyl-[acceptor protein]-L-lysine.

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei93Glycyl thioester intermediate1
Sitei100 – 101Substrate bindingBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Transferase
Biological processCell cycle, Cell division, Chromosome partition, Host-virus interaction, Mitosis, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-3065678 SUMO is transferred from E1 to E2 (UBE2I, UBC9)
R-MMU-3108214 SUMOylation of DNA damage response and repair proteins
R-MMU-3232118 SUMOylation of transcription factors
R-MMU-3899300 SUMOylation of transcription cofactors
R-MMU-4551638 SUMOylation of chromatin organization proteins
R-MMU-4570464 SUMOylation of RNA binding proteins
R-MMU-4615885 SUMOylation of DNA replication proteins
R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-MMU-5696395 Formation of Incision Complex in GG-NER
R-MMU-8866904 Negative regulation of activity of TFAP2 (AP-2) family transcription factors
UniPathwayiUPA00886

Names & Taxonomyi

Protein namesi
Recommended name:
SUMO-conjugating enzyme UBC9 (EC:2.3.2.-)
Alternative name(s):
RING-type E3 SUMO transferase UBC9
SUMO-protein ligase
Ubiquitin carrier protein 9
Short name:
mUBC9
Ubiquitin carrier protein I
Ubiquitin-conjugating enzyme E2 I
Ubiquitin-protein ligase I
Gene namesi
Name:Ube2i
Synonyms:Ubc9, Ubce2i, Ubce9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:107365 Ube2i

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Death prior to E7.5 due to major defects in nuclear organization.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000824562 – 158SUMO-conjugating enzyme UBC9Add BLAST157

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Cross-linki18Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki18Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei65N6-acetyllysineBy similarity1
Modified residuei71Phosphoserine; by CDK1By similarity1
Cross-linki101Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Phosphorylation at Ser-71 significantly enhances SUMOylation activity.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP63280
MaxQBiP63280
PaxDbiP63280
PeptideAtlasiP63280
PRIDEiP63280
TopDownProteomicsiP63280

PTM databases

iPTMnetiP63280
PhosphoSitePlusiP63280

Expressioni

Tissue specificityi

Present in spleen, kidney, lung, brain, heart and testis (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000015120
CleanExiMM_UBE2I
ExpressionAtlasiP63280 baseline and differential
GenevisibleiP63280 MM

Interactioni

Subunit structurei

Forms a tight complex with RANGAP1 and RANBP2. Interacts with SIAH1 and PARP. Interacts with various transcription factors such as TFAP2A, TFAP2B, TFAP2C, AR, ETS1 and SOX4. Interacts with RASD2. Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B. Interacts with FOXL2. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Interacts with DNM1L (via its GTPase and B domains); the interaction promotes sumoylation of DNM1L, mainly in the B domain (By similarity). Interacts with HIPK1, HIPK2, PPM1J and TCF3. Interacts with Moloney murine leukemia virus CA and adenovirus type 5 and type 12 E1A. Interacts with NR2C1; the interaction promotes its sumoylation. Interacts with NFATC2IP; this inhibits formation of poly-SUMO chains. Interacts with FHIT (By similarity). Interacts with PRKRA and p53/TP53. Interacts with UHRF2. Interacts with NR3C1 and this interaction is enhanced in the presence of RWDD3. Interacts with MTA1 (By similarity). Interacts with ZNF451. Identified in a complex with SUMO2 and UBC9, where one ZNF451 interacts with one UBC9 and two SUMO2 chains, one bound to the UBC9 active site and the other to another region of the same UBC9 molecule. Interacts with SETX (By similarity). Interacts with CPEB3 (PubMed:26074071).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei4Interaction with RANBP2By similarity1
Sitei25Interaction with RANBP2By similarity1
Sitei57Interaction with RANBP2By similarity1

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204408, 82 interactors
ComplexPortaliCPX-3052 sumoylated E2 ligase complex (SUMO1)
CPX-3053 sumoylated E2 ligase complex (SUMO2)
CPX-3054 sumoylated E2 ligase complex (SUMO3)
DIPiDIP-29276N
IntActiP63280, 57 interactors
MINTiP63280
STRINGi10090.ENSMUSP00000055714

Structurei

Secondary structure

1158
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 18Combined sources15
Beta strandi25 – 30Combined sources6
Beta strandi36 – 46Combined sources11
Turni52 – 55Combined sources4
Beta strandi57 – 63Combined sources7
Turni66 – 69Combined sources4
Beta strandi74 – 79Combined sources6
Beta strandi90 – 92Combined sources3
Helixi95 – 97Combined sources3
Turni99 – 102Combined sources4
Helixi109 – 121Combined sources13
Helixi131 – 139Combined sources9
Helixi141 – 154Combined sources14

3D structure databases

ProteinModelPortaliP63280
SMRiP63280
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63280

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 18Interaction with SUMO1By similarity6

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0424 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00550000075088
HOGENOMiHOG000233454
HOVERGENiHBG063308
InParanoidiP63280
KOiK10577
OMAiKCKFTPP
OrthoDBiEOG091G0NAK
PhylomeDBiP63280
TreeFamiTF101122

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR027230 Ubc9
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PANTHERiPTHR43927 PTHR43927, 1 hit
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG
60 70 80 90 100
TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED
110 120 130 140 150
KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA

QAKKFAPS
Length:158
Mass (Da):18,007
Last modified:October 11, 2004 - v1
Checksum:iE2C826E9C8D0683D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94402 mRNA Translation: AAB52424.1
X99739 mRNA Translation: CAA68072.1
U31934 mRNA Translation: AAB02182.1
U76416 mRNA Translation: AAB18790.1
U82627 mRNA Translation: AAB48446.1
X97575 mRNA Translation: CAA66188.1
AK003141 mRNA Translation: BAB22599.1
AK005058 mRNA Translation: BAB23783.1
AK011239 mRNA Translation: BAB27487.1
AK012282 mRNA Translation: BAB28140.1
AK088508 mRNA Translation: BAC40395.1
AK150575 mRNA Translation: BAE29670.1
AK160014 mRNA Translation: BAE35560.1
AK160988 mRNA Translation: BAE36134.1
AK165606 mRNA Translation: BAE38290.1
AK165615 mRNA Translation: BAE38295.1
AK166016 mRNA Translation: BAE38521.1
AK166657 mRNA Translation: BAE38922.1
AK166930 mRNA Translation: BAE39124.1
AK167162 mRNA Translation: BAE39303.1
AK168212 mRNA Translation: BAE40170.1
AK168706 mRNA Translation: BAE40548.1
AK168766 mRNA Translation: BAE40602.1
AY491413 Genomic DNA Translation: AAS21651.1
CCDSiCCDS28513.1
RefSeqiNP_001171080.1, NM_001177609.1
NP_001171081.1, NM_001177610.1
NP_035795.1, NM_011665.4
XP_006524111.1, XM_006524048.3
XP_006524112.1, XM_006524049.3
UniGeneiMm.240044
Mm.384234
Mm.442797

Genome annotation databases

EnsembliENSMUST00000049911; ENSMUSP00000055714; ENSMUSG00000015120
ENSMUST00000172618; ENSMUSP00000134169; ENSMUSG00000015120
ENSMUST00000173084; ENSMUSP00000134261; ENSMUSG00000015120
ENSMUST00000173713; ENSMUSP00000134491; ENSMUSG00000015120
ENSMUST00000174001; ENSMUSP00000134450; ENSMUSG00000015120
ENSMUST00000174031; ENSMUSP00000134350; ENSMUSG00000015120
GeneIDi22196
KEGGimmu:22196
UCSCiuc008bai.2 mouse

Similar proteinsi

Entry informationi

Entry nameiUBC9_MOUSE
AccessioniPrimary (citable) accession number: P63280
Secondary accession number(s): P50550
, Q15698, Q6RUT3, Q86VB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: July 18, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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