UniProtKB - P63271 (SPT4A_MOUSE)
Transcription elongation factor SPT4-A
Supt4h1a
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 16 – 36 | C4-typeSequence analysisAdd BLAST | 21 |
GO - Molecular functioni
- protein heterodimerization activity Source: MGI
- RNA polymerase II complex binding Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- chromatin organization Source: GO_Central
- mRNA processing Source: GO_Central
- negative regulation of DNA-templated transcription, elongation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- negative regulation of transcription elongation from RNA polymerase II promoter Source: MGI
- positive regulation of DNA-templated transcription, elongation Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription elongation from RNA polymerase II promoter Source: GO_Central
- transcription elongation from RNA polymerase II promoter Source: MGI
Keywordsi
Molecular function | Activator, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-112382, Formation of RNA Pol II elongation complex R-MMU-113418, Formation of the Early Elongation Complex R-MMU-674695, RNA Polymerase II Pre-transcription Events R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes R-MMU-6807505, RNA polymerase II transcribes snRNA genes R-MMU-75955, RNA Polymerase II Transcription Elongation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription elongation factor SPT4-AAlternative name(s): DRB sensitivity-inducing factor small subunit 1 Short name: DSIF small subunit 1 Transcription elongation factor SPT4 1 |
Gene namesi | Name:Supt4h1a Synonyms:Spt4h, Supt4a, Supt4h |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107416, Supt4a |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- DSIF complex Source: UniProtKB
- nucleoplasm Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000210327 | 2 – 117 | Transcription elongation factor SPT4-AAdd BLAST | 116 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | P63271 |
MaxQBi | P63271 |
PaxDbi | P63271 |
PeptideAtlasi | P63271 |
PRIDEi | P63271 |
PTM databases
iPTMneti | P63271 |
PhosphoSitePlusi | P63271 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000020485, Expressed in morula and 309 other tissues |
ExpressionAtlasi | P63271, baseline and differential |
Genevisiblei | P63271, MM |
Interactioni
Subunit structurei
Interacts with SUPT5H to form DSIF. DSIF interacts with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of CDK9 and cyclin-T (CCNT1 or CCNT2). DSIF interacts with RNA polymerase II, and this interaction is reduced by phosphorylation of the C-terminal domain (CTD) of POLR2A by P-TEFb. DSIF also interacts with the NELF complex, which is composed of WHSC2/NELFA, COBRA1/NELFB, TH1L/NELFD and RDBP/NELFE, and this interaction occurs following prior binding of DSIF to RNA polymerase II. DSIF also interacts with HRMT1L2/PRMT1, HTATSF1/TATSF1, RNGTT/CAP1A, SKB1/PRMT5, SUPT6H, and can interact with PIN1 (By similarity).
By similarityGO - Molecular functioni
- protein heterodimerization activity Source: MGI
- RNA polymerase II complex binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 203573, 2 interactors |
STRINGi | 10090.ENSMUSP00000091487 |
Miscellaneous databases
RNActi | P63271, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 40 | Interaction with SUPT5HBy similarityAdd BLAST | 39 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 16 – 36 | C4-typeSequence analysisAdd BLAST | 21 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3490, Eukaryota |
GeneTreei | ENSGT00390000018559 |
HOGENOMi | CLU_138052_3_0_1 |
InParanoidi | P63271 |
OMAi | VRGMYAV |
OrthoDBi | 1617752at2759 |
PhylomeDBi | P63271 |
TreeFami | TF300105 |
Family and domain databases
CDDi | cd07973, Spt4, 1 hit |
Gene3Di | 3.30.40.210, 1 hit |
InterProi | View protein in InterPro IPR029040, RPABC4/Spt4 IPR009287, Spt4 IPR022800, Spt4/RpoE2_Znf IPR038510, Spt4_sf |
PANTHERi | PTHR12882, PTHR12882, 1 hit |
Pfami | View protein in Pfam PF06093, Spt4, 1 hit |
PIRSFi | PIRSF025023, Spt4, 1 hit |
SMARTi | View protein in SMART SM01389, Spt4, 1 hit |
SUPFAMi | SSF63393, SSF63393, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MALETVPKDL RHLRACLLCS LVKTIDQFEY DGCDNCDAYL QMKGNREMVY
60 70 80 90 100
DCTSSSFDGI IAMMSPEDSW VSKWQRVSNF KPGVYAVSVT GRLPQGIVRE
110
LKSRGVAYKS RDTAIKT
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RHM5 | D6RHM5_MOUSE | SPT4A, DSIF elongation factor subun... | Supt4a Supt4h1 | 104 | Annotation score: | ||
G3UVU8 | G3UVU8_MOUSE | SPT4A, DSIF elongation factor subun... | Supt4a Supt4h1 | 87 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 11 | R → L in BAE39944 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43154 mRNA Translation: AAB18730.1 U96809 Genomic DNA Translation: AAC71659.1 AK167941 mRNA Translation: BAE39944.1 AL604022 Genomic DNA No translation available. CU393486 Genomic DNA No translation available. CH466556 Genomic DNA Translation: EDL15830.1 BC024391 mRNA Translation: AAH24391.1 BC061174 mRNA Translation: AAH61174.1 BC087923 mRNA Translation: AAH87923.1 BC141092 mRNA Translation: AAI41093.1 |
CCDSi | CCDS36270.1 |
RefSeqi | NP_033322.1, NM_009296.1 |
Genome annotation databases
Ensembli | ENSMUST00000093955; ENSMUSP00000091487; ENSMUSG00000020485 |
GeneIDi | 20922 |
KEGGi | mmu:20922 |
UCSCi | uc007kuj.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43154 mRNA Translation: AAB18730.1 U96809 Genomic DNA Translation: AAC71659.1 AK167941 mRNA Translation: BAE39944.1 AL604022 Genomic DNA No translation available. CU393486 Genomic DNA No translation available. CH466556 Genomic DNA Translation: EDL15830.1 BC024391 mRNA Translation: AAH24391.1 BC061174 mRNA Translation: AAH61174.1 BC087923 mRNA Translation: AAH87923.1 BC141092 mRNA Translation: AAI41093.1 |
CCDSi | CCDS36270.1 |
RefSeqi | NP_033322.1, NM_009296.1 |
3D structure databases
SMRi | P63271 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203573, 2 interactors |
STRINGi | 10090.ENSMUSP00000091487 |
PTM databases
iPTMneti | P63271 |
PhosphoSitePlusi | P63271 |
Proteomic databases
EPDi | P63271 |
MaxQBi | P63271 |
PaxDbi | P63271 |
PeptideAtlasi | P63271 |
PRIDEi | P63271 |
Protocols and materials databases
Antibodypediai | 18387, 159 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000093955; ENSMUSP00000091487; ENSMUSG00000020485 |
GeneIDi | 20922 |
KEGGi | mmu:20922 |
UCSCi | uc007kuj.1, mouse |
Organism-specific databases
CTDi | 20922 |
MGIi | MGI:107416, Supt4a |
Phylogenomic databases
eggNOGi | KOG3490, Eukaryota |
GeneTreei | ENSGT00390000018559 |
HOGENOMi | CLU_138052_3_0_1 |
InParanoidi | P63271 |
OMAi | VRGMYAV |
OrthoDBi | 1617752at2759 |
PhylomeDBi | P63271 |
TreeFami | TF300105 |
Enzyme and pathway databases
Reactomei | R-MMU-112382, Formation of RNA Pol II elongation complex R-MMU-113418, Formation of the Early Elongation Complex R-MMU-674695, RNA Polymerase II Pre-transcription Events R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes R-MMU-6807505, RNA polymerase II transcribes snRNA genes R-MMU-75955, RNA Polymerase II Transcription Elongation |
Miscellaneous databases
BioGRID-ORCSi | 20922, 7 hits in 20 CRISPR screens |
ChiTaRSi | Supt4a, mouse |
PROi | PR:P63271 |
RNActi | P63271, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020485, Expressed in morula and 309 other tissues |
ExpressionAtlasi | P63271, baseline and differential |
Genevisiblei | P63271, MM |
Family and domain databases
CDDi | cd07973, Spt4, 1 hit |
Gene3Di | 3.30.40.210, 1 hit |
InterProi | View protein in InterPro IPR029040, RPABC4/Spt4 IPR009287, Spt4 IPR022800, Spt4/RpoE2_Znf IPR038510, Spt4_sf |
PANTHERi | PTHR12882, PTHR12882, 1 hit |
Pfami | View protein in Pfam PF06093, Spt4, 1 hit |
PIRSFi | PIRSF025023, Spt4, 1 hit |
SMARTi | View protein in SMART SM01389, Spt4, 1 hit |
SUPFAMi | SSF63393, SSF63393, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPT4A_MOUSE | |
Accessioni | P63271Primary (citable) accession number: P63271 Secondary accession number(s): B2KGH6 Q62387 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | December 2, 2020 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families