RACK1

Functionⁱ

Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression (PubMed:23636399). Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits (PubMed:28132843). Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (PubMed:19674157). Promotes migration of breast carcinoma cells by binding to and activating RHOA (PubMed:20499158).21 Publications

(Microbial infection) Binds to Y.pseudotuberculosis yopK which leads to inhibition of phagocytosis and survival of bacteria following infection of host cells.1 Publication

(Microbial infection) Enhances phosphorylation of HIV-1 Nef by PKCs.1 Publication

(Microbial infection) In case of poxvirus infection, remodels the ribosomes so that they become optimal for the viral mRNAs (containing poly-A leaders) translation but not for host mRNAs.1 Publication

(Microbial infection) Contributes to the cap-independent internal ribosome entry site (IRES)-mediated translation by some RNA viruses.1 Publication

GO - Molecular functionⁱ

cadherin binding
Source: BHF-UCL
Inferred from high throughput direct assayⁱ
- 25468996
cyclin binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 15159402
cysteine-type endopeptidase activator activity involved in apoptotic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19767770
enzyme binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 20819076
ion channel inhibitor activity Source: UniProtKB
protein-containing complex scaffold activity
Source: BHF-UCL
Traceable author statementⁱ
- 20819076
protein homodimerization activity
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 24947010
protein kinase C binding
Source: UniProtKB
Inferred from direct assayⁱ
- 11279199
protein phosphatase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 11278757
protein tyrosine kinase inhibitor activity
Source: UniProtKB
Inferred from direct assayⁱ
- 9584165
receptor tyrosine kinase binding
Source: UniProtKB
Inferred from direct assayⁱ
- 9584165
ribosome binding
Source: UniProtKB
Inferred from direct assayⁱ
- 28132843
RNA binding
Source: UniProtKB
Inferred from high throughput direct assayⁱ
- 22658674
- 22681889
SH2 domain binding
Source: UniProtKB
Inferred from direct assayⁱ
- 11279199
- 9584165
signaling adaptor activity
Source: ParkinsonsUK-UCL
Inferred from mutant phenotypeⁱ
- 20103773
signaling receptor binding
Source: UniProtKB
Non-traceable author statementⁱ
- 12809483

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

activation of cysteine-type endopeptidase activity involved in apoptotic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19767770
cell cycle Source: UniProtKB-KW
cellular response to glucose stimulus
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 20103773
cellular response to growth factor stimulus
Source: UniProtKB
Inferred from direct assayⁱ
- 20010870
gastrulation Source: UniProtKB-KW
negative regulation of cell growth
Source: UniProtKB
Inferred from direct assayⁱ
- 9584165
negative regulation of endoplasmic reticulum unfolded protein response
Source: ParkinsonsUK-UCL
Traceable author statementⁱ
- 22013210
negative regulation of gene expression
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19451233
negative regulation of hydrogen peroxide-induced neuron death
Source: ParkinsonsUK-UCL
Inferred from genetic interactionⁱ
- 24947010
negative regulation of peptidyl-serine phosphorylation
Source: ParkinsonsUK-UCL
Inferred from mutant phenotypeⁱ
- 20103773
negative regulation of phagocytosis
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21347310
negative regulation of protein kinase B signaling
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19767770
negative regulation of translation Source: UniProtKB
negative regulation of Wnt signaling pathway Source: UniProtKB
pigmentation Source: Ensembl
positive regulation of apoptotic process
Source: UniProtKB
Inferred from direct assayⁱ
- 20541605
positive regulation of cell migration
Source: UniProtKB
Inferred from direct assayⁱ
- 20499158
positive regulation of ceramide biosynthetic process Source: Reactome
positive regulation of cyclic-nucleotide phosphodiesterase activity
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 20819076
positive regulation of gastrulation Source: UniProtKB
positive regulation of Golgi to plasma membrane protein transport
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19674157
positive regulation of GTPase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 20499158
positive regulation of intrinsic apoptotic signaling pathway
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19767770
positive regulation of mitochondrial depolarization
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19767770
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
Source: UniProtKB
Inferred from direct assayⁱ
- 19785988
positive regulation of protein homooligomerization
Source: UniProtKB
Inferred from direct assayⁱ
- 20541605
positive regulation of protein phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 11312657
- 17108144
protein ubiquitination
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 28132843
regulation of cell cycle
Source: UniProtKB
Inferred from direct assayⁱ
- 9584165
regulation of cell division Source: UniProtKB
regulation of establishment of cell polarity Source: UniProtKB
regulation of protein localization Source: UniProtKB
regulation of tumor necrosis factor-mediated signaling pathway Source: Reactome
rescue of stalled ribosome
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 28132843
rhythmic process Source: UniProtKB-KW
viral process Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Developmental protein, Ribonucleoprotein, Ribosomal protein
Biological process	Apoptosis, Biological rhythms, Cell cycle, Gastrulation, Growth regulation, Host-virus interaction, Translation regulation

Enzyme and pathway databases

Reactomeⁱ	R-HSA-5357905 Regulation of TNFR1 signaling R-HSA-5357956 TNFR1-induced NFkappaB signaling pathway R-HSA-5626978 TNFR1-mediated ceramide production
SignaLinkⁱ	P63244
SIGNORⁱ	P63244

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Receptor of activated protein C kinase 1 Alternative name(s): Cell proliferation-inducing gene 21 protein Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Short name: HLC-7 Receptor for activated C kinase Small ribosomal subunit protein RACK11 Publication Cleaved into the following chain: Receptor of activated protein C kinase 1, N-terminally processed Alternative name(s): Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed
Gene namesⁱ	Name:RACK1Imported Synonyms:GNB2L1 ORF Names:HLC7, PIG21
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 5

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000204628.11
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:4399 RACK1
MIMⁱ	176981 gene
neXtProtⁱ	NX_P63244

Keywords - Cellular componentⁱ

Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	36 – 38	RDK → DDE in DEmut; abolishes association with the ribosome and ability to initiate the ribosome quality control (RQC). 1 Publication	3
Mutagenesisⁱ	52	Y → F: No effect on binding to SRC. Abolishes binding to PTK2/FAK1 and reduces cell adhesion and foci formation. 3 Publications	1
Mutagenesisⁱ	57	R → A: Decreased binding to PTK2/FAK1; when associated with A-60. 1 Publication	1
Mutagenesisⁱ	60	R → A: Decreased binding to PTK2/FAK1; when associated with A-57. 1 Publication	1
Mutagenesisⁱ	127	K → A: Decreased binding to PTK2/FAK1; when associated with A-130. 1 Publication	1
Mutagenesisⁱ	130	K → A: Decreased binding to PTK2/FAK1; when associated with A-127. 1 Publication	1
Mutagenesisⁱ	140	Y → F: No effect on binding to SRC. 2 Publications	1
Mutagenesisⁱ	194	Y → F: No effect on binding to SRC. 2 Publications	1
Mutagenesisⁱ	228	Y → F: No effect on binding to SRC. Does not abolish phosphorylation by SRC. Abolishes phosphorylation by SRC; when associated with F-246 and F-302. 2 Publications	1
Mutagenesisⁱ	246	Y → F: Abolishes binding to SRC. Does not abolish phosphorylation by SRC. Abolishes phosphorylation by SRC; when associated with F-228 and F-302. 2 Publications	1
Mutagenesisⁱ	276 – 279	STSS → EEEE: Enhanced translation of mRNAs containing poly-A leaders. 1 Publication	4
Mutagenesisⁱ	278	S → E: Enhanced translation of mRNAs containing poly-A leaders. 1 Publication	1
Mutagenesisⁱ	302	Y → F: No effect on binding to SRC. Abolishes phosphorylation by SRC; when associated with F-228 and F-246. 2 Publications	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	10399
Open Targets More...OpenTargetsⁱ	ENSG00000204628
PharmGKBⁱ	PA28779

Polymorphism and mutation databases

BioMutaⁱ	GNB2L1
DMDMⁱ	54037168

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
ChainⁱPRO_0000424480	1 – 317	Receptor of activated protein C kinase 1Add BLAST	317
Initiator methionineⁱ		Removed; alternateCombined sources1 Publication
ChainⁱPRO_0000127731	2 – 317	Receptor of activated protein C kinase 1, N-terminally processedAdd BLAST	316

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	1	N-acetylmethionineCombined sources	1
Modified residueⁱ	2	N-acetylthreonine; in Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processedCombined sources1 Publication	1
Modified residueⁱ	6	PhosphothreonineCombined sources	1
Modified residueⁱ	10	PhosphothreonineCombined sources	1
Modified residueⁱ	52	Phosphotyrosine; by ABL11 Publication	1
Modified residueⁱ	96	PhosphothreonineCombined sources	1
Modified residueⁱ	130	N6-acetyllysineCombined sources	1
Modified residueⁱ	183	N6-acetyllysineBy similarity	1
Modified residueⁱ	228	Phosphotyrosine1 Publication	1
Modified residueⁱ	276	Phosphoserine; by viral VacV B1 kinaseCombined sources1 Publication	1
Modified residueⁱ	277	Phosphothreonine; by viral VacV B1 kinase1 Publication	1
Modified residueⁱ	278	Phosphoserine; by viral VacV B1 kinase1 Publication	1
Modified residueⁱ	279	Phosphoserine; by viral VacV B1 kinase1 Publication	1
Modified residueⁱ	316	PhosphothreonineBy similarity	1

Post-translational modificationⁱ

Phosphorylated on Tyr-228 and/or Tyr-246 by SRC. This is required for binding to SRC.3 Publications

(Microbial infection) Phosphorylated by vaccinia virus B1 kinase on serine and threonine residues; this phosphorylation remodels the ribosome properties, favoring the viral mRNA translation.1 Publication

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	P63244
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P63244
PaxDbⁱ	P63244
PeptideAtlas More...PeptideAtlasⁱ	P63244
PRIDEⁱ	P63244
ProteomicsDBⁱ	57512
TopDownProteomicsⁱ	P63244

2D gel databases

REPRODUCTION-2DPAGE More...REPRODUCTION-2DPAGEⁱ	IPI00848226 P63244

REPRODUCTION-2DPAGEⁱ

IPI00848226
P63244

PTM databases

iPTMnetⁱ	P63244
PhosphoSitePlusⁱ	P63244
SwissPalmⁱ	P63244

Expressionⁱ

Tissue specificityⁱ

In the liver, expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. Up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000204628 Expressed in 246 organ(s), highest expression level in female gonad
CleanExⁱ	HS_GNB2L1
ExpressionAtlasⁱ	P63244 baseline and differential
Genevisibleⁱ	P63244 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB004288 HPA021676

HPAⁱ

CAB004288
HPA021676

Interactionⁱ

Subunit structureⁱ

Monomer; also forms homodimers and homooligomers (PubMed:20529362, PubMed:15140893). Interacts with CPNE3 (PubMed:20010870). May interact with ABCB4 (PubMed:19674157). Component of the small (40S) ribosomal subunit (By similarity). Interacts with the 80S ribosome (PubMed:23636399). Binds SLC9A3R1. Forms a ternary complex with TRIM63 and PRKCE. Interacts with HABP4, KRT1 and OTUB1. Interacts with SRC (via SH2 domain); the interaction is enhanced by tyrosine phosphorylation of RACK1. Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and PRKCA. Interacts with AR. Interacts with IGF1R but not with INSR. Interacts with ADAM12. Interacts with CLEC1B (via N-terminal region) and with HIF1A; the interaction promotes their degradation. Interacts with RHOA; this enhances RHOA activation and promotes cell migration. Interacts with CHRM2; the interaction regulates CHRM2 internalization. Interacts with TRPM6 (via kinase domain). Interacts with PTK2/FAK1; required for PTK2/FAK1 phosphorylation and dephosphorylation. Interacts with FLT1. Interacts with TBXA2R isoform 2. Interacts with HRAS. Interacts with LARP4B. Interacts with LARP4 (PubMed:21098120). Interacts with PKD2L1.By similarity28 Publications

(Microbial infection) Interacts with Y.pseudotuberculosis yopK.1 Publication

(Microbial infection) Interacts with Y.pseudotuberculosis yopK. Interacts with a number of viral proteins including Epstein-Barr virus BZLF1 and HIV-1 Nef; interaction with Nef increases Nef phosphorylation by PKC.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
ACHE	P22303	2	EBI-296739,EBI-1637793
ACTN2	P35609	3	EBI-296739,EBI-77797
AGO2	Q9UKV8	2	EBI-296739,EBI-528269
BCL2L11	O43521	2	EBI-296739,EBI-526406
Bcl2l11	O54918-1	2	EBI-296739,EBI-526076	From a different organism.
BZLF1	P03206	5	EBI-296739,EBI-2621186	From Epstein-Barr virus (strain B95-8).
DYNLL1	P63167	6	EBI-296739,EBI-349105
EED	O75530	3	EBI-296739,EBI-923794
IFNAR2	P48551	4	EBI-296739,EBI-958408
LARP4B	Q92615	3	EBI-296739,EBI-1052558
LRP12	Q9Y561	2	EBI-296739,EBI-296693
LYN	P07948	2	EBI-296739,EBI-79452
PAG1	Q9NWQ8	2	EBI-296739,EBI-2828115
PARK7	Q99497	4	EBI-296739,EBI-1164361
PIAS2	O75928	2	EBI-296739,EBI-348555
PPID	Q08752	4	EBI-296739,EBI-716596
VHL	P40337	9	EBI-296739,EBI-301246

GO - Molecular functionⁱ

cadherin binding
Source: BHF-UCL
Inferred from high throughput direct assayⁱ
- 25468996
cyclin binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 15159402
enzyme binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 20819076
protein-containing complex scaffold activity
Source: BHF-UCL
Traceable author statementⁱ
- 20819076
protein homodimerization activity
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 24947010
protein kinase C binding
Source: UniProtKB
Inferred from direct assayⁱ
- 11279199
protein phosphatase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 11278757
receptor tyrosine kinase binding
Source: UniProtKB
Inferred from direct assayⁱ
- 9584165
SH2 domain binding
Source: UniProtKB
Inferred from direct assayⁱ
- 11279199
- 9584165
signaling receptor binding
Source: UniProtKB
Non-traceable author statementⁱ
- 12809483

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	115671, 216 interactors
CORUMⁱ	P63244
Database of interacting proteins More...DIPⁱ	DIP-46736N
IntActⁱ	P63244, 104 interactors
Molecular INTeraction database More...MINTⁱ	P63244
STRINGⁱ	9606.ENSP00000426909

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	4 – 11	Combined sources	8
Beta strandⁱ	18 – 23	Combined sources	6
Beta strandⁱ	30 – 35	Combined sources	6
Beta strandⁱ	40 – 45	Combined sources	6
Beta strandⁱ	48 – 50	Combined sources	3
Beta strandⁱ	52 – 59	Combined sources	8
Beta strandⁱ	66 – 71	Combined sources	6
Beta strandⁱ	75 – 82	Combined sources	8
Beta strandⁱ	85 – 91	Combined sources	7
Turnⁱ	92 – 95	Combined sources	4
Beta strandⁱ	96 – 102	Combined sources	7
Beta strandⁱ	108 – 113	Combined sources	6
Beta strandⁱ	120 – 124	Combined sources	5
Beta strandⁱ	129 – 132	Combined sources	4
Beta strandⁱ	138 – 142	Combined sources	5
Beta strandⁱ	144 – 146	Combined sources	3
Beta strandⁱ	151 – 156	Combined sources	6
Beta strandⁱ	160 – 162	Combined sources	3
Beta strandⁱ	164 – 169	Combined sources	6
Beta strandⁱ	174 – 178	Combined sources	5
Turnⁱ	179 – 182	Combined sources	4
Beta strandⁱ	183 – 188	Combined sources	6
Beta strandⁱ	195 – 200	Combined sources	6
Beta strandⁱ	204 – 211	Combined sources	8
Beta strandⁱ	215 – 220	Combined sources	6
Turnⁱ	221 – 224	Combined sources	4
Beta strandⁱ	225 – 231	Combined sources	7
Beta strandⁱ	236 – 241	Combined sources	6
Beta strandⁱ	243 – 252	Combined sources	10
Beta strandⁱ	255 – 260	Combined sources	6
Turnⁱ	261 – 264	Combined sources	4
Beta strandⁱ	265 – 270	Combined sources	6
Beta strandⁱ	286 – 291	Combined sources	6
Beta strandⁱ	295 – 302	Combined sources	8
Beta strandⁱ	307 – 313	Combined sources	7

Show more details

3D structure databases

ProteinModelPortalⁱ	P63244
SMRⁱ	P63244
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Repeatⁱ	13 – 44	WD 1Add BLAST	32
Repeatⁱ	61 – 91	WD 2Add BLAST	31
Repeatⁱ	103 – 133	WD 3Add BLAST	31
Repeatⁱ	146 – 178	WD 4Add BLAST	33
Repeatⁱ	190 – 220	WD 5Add BLAST	31
Repeatⁱ	231 – 260	WD 6Add BLAST	30
Repeatⁱ	281 – 311	WD 7Add BLAST	31

Domainⁱ

The 7 WD repeats mediate protein-protein interactions with binding partners.1 Publication

Sequence similaritiesⁱ

Belongs to the WD repeat G protein beta family. Ribosomal protein RACK1 subfamily.Curated

Keywords - Domainⁱ

Repeat, WD repeat

Phylogenomic databases

eggNOGⁱ	KOG0279 Eukaryota ENOG410XQGZ LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00910000144232
HOVERGENⁱ	HBG000277
InParanoidⁱ	P63244
KEGG Orthology (KO) More...KOⁱ	K14753
OMAⁱ	QYGYPKR
PhylomeDBⁱ	P63244
TreeFamⁱ	TF300600

Family and domain databases

Gene3Dⁱ	2.130.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR020472 G-protein_beta_WD-40_rep IPR015943 WD40/YVTN_repeat-like_dom_sf IPR001680 WD40_repeat IPR019775 WD40_repeat_CS IPR017986 WD40_repeat_dom IPR036322 WD40_repeat_dom_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00400 WD40, 7 hits
PRINTSⁱ	PR00320 GPROTEINBRPT
SMARTⁱ	View protein in SMART SM00320 WD40, 7 hits
SUPFAMⁱ	SSF50978 SSF50978, 1 hit
PROSITEⁱ	View protein in PROSITE PS00678 WD_REPEATS_1, 4 hits PS50082 WD_REPEATS_2, 6 hits PS50294 WD_REPEATS_REGION, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 21 potential isoforms that are computationally mapped.ⁱShow all

P63244-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET 
        60         70         80         90        100
NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR 
       110        120        130        140        150
FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW 
       160        170        180        190        200
VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA NCKLKTNHIG HTGYLNTVTV 
       210        220        230        240        250
SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC FSPNRYWLCA 
       260        270        280        290        300
ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA 
       310 
GYTDNLVRVW QVTIGTR

Length:317

Mass (Da):35,077

Last modified:January 23, 2007 - v3

Checksum:ⁱ257F91E369ED2044

GO

Computationally mapped potential isoform sequencesⁱ

There are 21 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name	Protein names	Gene names	Length	Annotation
	D6RFX4	D6RFX4_HUMAN	Receptor of-activated protein C kin... Receptor of-activated protein C kinase 1	RACK1	194	Annotation score:
	D6REE5	D6REE5_HUMAN	Receptor of-activated protein C kin... Receptor of-activated protein C kinase 1	RACK1	321	Annotation score:
	D6R9L0	D6R9L0_HUMAN	Receptor of-activated protein C kin... Receptor of-activated protein C kinase 1	RACK1	300	Annotation score:
	D6RAC2	D6RAC2_HUMAN	Receptor of-activated protein C kin... Receptor of-activated protein C kinase 1	RACK1	269	Annotation score:
	D6R9Z1	D6R9Z1_HUMAN	Receptor of-activated protein C kin... Receptor of-activated protein C kinase 1	RACK1	236	Annotation score:
	H0YAM7	H0YAM7_HUMAN	Receptor of-activated protein C kin... Receptor of-activated protein C kinase 1	RACK1	247	Annotation score:
	H0Y8W2	H0Y8W2_HUMAN	Receptor of-activated protein C kin... Receptor of-activated protein C kinase 1	RACK1	274	Annotation score:
	J3KPE3	J3KPE3_HUMAN	Receptor of-activated protein C kin... Receptor of-activated protein C kinase 1	RACK1	273	Annotation score:
	H0Y8R5	H0Y8R5_HUMAN	Receptor of-activated protein C kin... Receptor of-activated protein C kinase 1	RACK1	138	Annotation score:
	D6RHH4	D6RHH4_HUMAN	Receptor of-activated protein C kin... Receptor of-activated protein C kinase 1	RACK1	233	Annotation score:
There are more potential isoformsShow all

Sequence cautionⁱ

The sequence AAO21313 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

The sequence AAR24619 differs from that shown. Reason: Frameshift at position 94.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	70 – 111	Missing in BAG53102 (PubMed:14702039).CuratedAdd BLAST	42
Sequence conflictⁱ	94	T → TRK in AAR24619 (Ref. 3) Curated	1
Sequence conflictⁱ	221	L → F in BAD96208 (Ref. 6) Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M24194 mRNA Translation: AAA59626.1 AY159316 mRNA Translation: AAO21313.1 Different initiation. AY336089 mRNA Translation: AAR24619.1 Frameshift. AK095666 mRNA Translation: BAG53102.1 CR456978 mRNA Translation: CAG33259.1 CR541909 mRNA Translation: CAG46707.1 AK222488 mRNA Translation: BAD96208.1 CH471165 Genomic DNA Translation: EAW53692.1 CH471165 Genomic DNA Translation: EAW53702.1 BC000214 mRNA Translation: AAH00214.1 BC000366 mRNA Translation: AAH00366.1 BC010119 mRNA Translation: AAH10119.1 BC014256 mRNA Translation: AAH14256.1 BC014788 mRNA Translation: AAH14788.1 BC017287 mRNA Translation: AAH17287.1 BC019093 mRNA Translation: AAH19093.1 BC019362 mRNA Translation: AAH19362.1 BC021993 mRNA Translation: AAH21993.1 BC032006 mRNA Translation: AAH32006.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS34324.1
PIRⁱ	B33928
NCBI Reference Sequences More...RefSeqⁱ	NP_006089.1, NM_006098.4
UniGeneⁱ	Hs.5662

Genome annotation databases

Ensemblⁱ	ENST00000512805; ENSP00000426909; ENSG00000204628
GeneIDⁱ	10399
KEGGⁱ	hsa:10399
UCSC genome browser More...UCSCⁱ	uc003mni.2 human

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P63244	Guanine nucleotide-binding protein subunit beta-2-like 1		MACFA		317	UniRef100_P63244
Receptor of activated protein C kinase 1		BOVIN		317
Receptor of activated protein C kinase 1		CHICK		317
Receptor of activated protein C kinase 1		MOUSE		317
Receptor of activated protein C kinase 1	)	PIG		317
+62

Protein	Similar proteins		Species	Score	Length	Source
P63244	Guanine nucleotide-binding protein subunit beta-2-like 1		MACFA		317	UniRef90_P63244
Receptor of activated protein C kinase 1		BOVIN		317
Receptor of activated protein C kinase 1		CHICK		317
Receptor of activated protein C kinase 1		MOUSE		317
Receptor of activated protein C kinase 1	)	PIG		317
+62

Protein	Similar proteins		Species	Score	Length	Source
P63244	Guanine nucleotide-binding protein subunit beta-2-like 1		MACFA		317	UniRef50_P63244
Receptor of activated protein C kinase 1		BOVIN		317
Receptor of activated protein C kinase 1		CHICK		317
Receptor of activated protein C kinase 1		MOUSE		317
Receptor of activated protein C kinase 1	)	PIG		317
+62

Cross-referencesⁱ

Web resourcesⁱ

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M24194 mRNA Translation: AAA59626.1 AY159316 mRNA Translation: AAO21313.1 Different initiation. AY336089 mRNA Translation: AAR24619.1 Frameshift. AK095666 mRNA Translation: BAG53102.1 CR456978 mRNA Translation: CAG33259.1 CR541909 mRNA Translation: CAG46707.1 AK222488 mRNA Translation: BAD96208.1 CH471165 Genomic DNA Translation: EAW53692.1 CH471165 Genomic DNA Translation: EAW53702.1 BC000214 mRNA Translation: AAH00214.1 BC000366 mRNA Translation: AAH00366.1 BC010119 mRNA Translation: AAH10119.1 BC014256 mRNA Translation: AAH14256.1 BC014788 mRNA Translation: AAH14788.1 BC017287 mRNA Translation: AAH17287.1 BC019093 mRNA Translation: AAH19093.1 BC019362 mRNA Translation: AAH19362.1 BC021993 mRNA Translation: AAH21993.1 BC032006 mRNA Translation: AAH32006.1
CCDSⁱ	CCDS34324.1
PIRⁱ	B33928
RefSeqⁱ	NP_006089.1, NM_006098.4
UniGeneⁱ	Hs.5662

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4AOW	X-ray	2.45	A/B/C	1-317	[»]
	4UG0	electron microscopy	-	Sg	1-317	[»]
	4V6X	electron microscopy	5.00	Ag	1-317	[»]
	5A2Q	electron microscopy	3.90	g	1-315	[»]
	5AJ0	electron microscopy	3.50	Bg	1-317	[»]
	5FLX	electron microscopy	3.90	g	1-317	[»]
	5LKS	electron microscopy	3.60	Sg	1-317	[»]
	5OA3	electron microscopy	4.30	g	1-315	[»]
	5T2C	electron microscopy	3.60	AI	1-317	[»]
	5VYC	X-ray	6.00	g1/g2/g3/g4/g5/g6	1-317	[»]
	6EK0	electron microscopy	2.90	Sg	1-317	[»]
	6FEC	electron microscopy	6.30	m	2-314	[»]
	6G18	electron microscopy	3.60	g	1-317	[»]
	6G51	electron microscopy	4.10	g	1-317	[»]
	6G53	electron microscopy	4.50	g	1-317	[»]
	6G5H	electron microscopy	3.60	g	1-317	[»]
	6G5I	electron microscopy	3.50	g	1-317	[»]
ProteinModelPortalⁱ	P63244
SMRⁱ	P63244
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	115671, 216 interactors
CORUMⁱ	P63244
DIPⁱ	DIP-46736N
IntActⁱ	P63244, 104 interactors
MINTⁱ	P63244
STRINGⁱ	9606.ENSP00000426909

PTM databases

iPTMnetⁱ	P63244
PhosphoSitePlusⁱ	P63244
SwissPalmⁱ	P63244

Polymorphism and mutation databases

BioMutaⁱ	GNB2L1
DMDMⁱ	54037168

2D gel databases

REPRODUCTION-2DPAGEⁱ	IPI00848226 P63244

REPRODUCTION-2DPAGEⁱ

IPI00848226
P63244

Proteomic databases

EPDⁱ	P63244
MaxQBⁱ	P63244
PaxDbⁱ	P63244
PeptideAtlasⁱ	P63244
PRIDEⁱ	P63244
ProteomicsDBⁱ	57512
TopDownProteomicsⁱ	P63244

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	10399
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000512805; ENSP00000426909; ENSG00000204628
GeneIDⁱ	10399
KEGGⁱ	hsa:10399
UCSCⁱ	uc003mni.2 human

Organism-specific databases

CTDⁱ	10399
DisGeNETⁱ	10399
EuPathDBⁱ	HostDB:ENSG00000204628.11
GeneCardsⁱ	RACK1
HGNCⁱ	HGNC:4399 RACK1
HPAⁱ	CAB004288 HPA021676
MIMⁱ	176981 gene
neXtProtⁱ	NX_P63244
OpenTargetsⁱ	ENSG00000204628
PharmGKBⁱ	PA28779
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0279 Eukaryota ENOG410XQGZ LUCA
GeneTreeⁱ	ENSGT00910000144232
HOVERGENⁱ	HBG000277
InParanoidⁱ	P63244
KOⁱ	K14753
OMAⁱ	QYGYPKR
PhylomeDBⁱ	P63244
TreeFamⁱ	TF300600

Enzyme and pathway databases

Reactomeⁱ	R-HSA-5357905 Regulation of TNFR1 signaling R-HSA-5357956 TNFR1-induced NFkappaB signaling pathway R-HSA-5626978 TNFR1-mediated ceramide production
SignaLinkⁱ	P63244
SIGNORⁱ	P63244

Miscellaneous databases

ChiTaRSⁱ	RACK1 human
GeneWikiⁱ	GNB2L1
GenomeRNAiⁱ	10399
Protein Ontology More...PROⁱ	PR:P63244
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000204628 Expressed in 246 organ(s), highest expression level in female gonad
CleanExⁱ	HS_GNB2L1
ExpressionAtlasⁱ	P63244 baseline and differential
Genevisibleⁱ	P63244 HS

Family and domain databases

Gene3Dⁱ	2.130.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR020472 G-protein_beta_WD-40_rep IPR015943 WD40/YVTN_repeat-like_dom_sf IPR001680 WD40_repeat IPR019775 WD40_repeat_CS IPR017986 WD40_repeat_dom IPR036322 WD40_repeat_dom_sf
Pfamⁱ	View protein in Pfam PF00400 WD40, 7 hits
PRINTSⁱ	PR00320 GPROTEINBRPT
SMARTⁱ	View protein in SMART SM00320 WD40, 7 hits
SUPFAMⁱ	SSF50978 SSF50978, 1 hit
PROSITEⁱ	View protein in PROSITE PS00678 WD_REPEATS_1, 4 hits PS50082 WD_REPEATS_2, 6 hits PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RACK1_HUMAN
Accessionⁱ	P63244Primary (citable) accession number: P63244 Secondary accession number(s): B3KTJ0 Q6FH47
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
	Last sequence update:	January 23, 2007
	Last modified:	September 12, 2018
	This is version 153 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

UniProtKB

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Supporting data

UniProtKB - P63244 (RACK1_HUMAN)

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Receptor of activated protein C kinase 1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Nucleus

Other locations

Cytosol

Extracellular region or secreted

Mitochondrion

Nucleus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Computationally mapped potential isoform sequencesi

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Computationally mapped potential isoform sequencesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ