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Protein

Eukaryotic translation initiation factor 5A-1

Gene

EIF5A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.5 Publications

GO - Molecular functioni

  • protein N-terminus binding Source: UniProtKB
  • ribosome binding Source: InterPro
  • RNA binding Source: UniProtKB
  • translation elongation factor activity Source: UniProtKB
  • U6 snRNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionElongation factor, RNA-binding
Biological processmRNA transport, Protein biosynthesis, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-204626 Hypusine synthesis from eIF5A-lysine

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5A-1
Short name:
eIF-5A-1
Short name:
eIF-5A1
Alternative name(s):
Eukaryotic initiation factor 5A isoform 1
Short name:
eIF-5A
Rev-binding factor
eIF-4D
Gene namesi
Name:EIF5A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000132507.17
HGNCiHGNC:3300 EIF5A
MIMi600187 gene
neXtProtiNX_P63241

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Biotechnological usei

Mature eIF5A-1 may be used as an in situ diagnostic marker for aberrant proliferation in intraepithelial neoplasia of the vulva.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47K → A or R: Abolishes acetylation. 2 Publications1
Mutagenesisi47K → D: Causes total inactivation of eIF5A in supporting yeast growth. 2 Publications1
Mutagenesisi49G → A: Causes total inactivation of eIF5A in supporting yeast growth. 1 Publication1
Mutagenesisi50K → A: Decreases significantly the acetylation at position K-47 and causes total inactivation of eIF5A in supporting yeast growth. 3 Publications1
Mutagenesisi50K → I, D or R: Causes total inactivation of eIF5A in supporting yeast growth. 3 Publications1
Mutagenesisi52G → A: Causes total inactivation of eIF5A in supporting yeast growth. 1 Publication1
Mutagenesisi55K → A: Causes total inactivation of eIF5A in supporting yeast growth. 1 Publication1
Mutagenesisi81V → G: Leads to temperature sensitivity when expressed in yeast cells. 1 Publication1

Organism-specific databases

DisGeNETi1984
OpenTargetsiENSG00000132507
PharmGKBiPA27726

Polymorphism and mutation databases

BioMutaiEIF5A
DMDMi54037409

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001424512 – 154Eukaryotic translation initiation factor 5A-1Add BLAST153

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei47N6-acetyllysine1 Publication1
Modified residuei50Hypusine1 Publication1
Modified residuei121N6-acetyllysineBy similarity1

Post-translational modificationi

Acetylated. Deacetylated by SIRT2.3 Publications
eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

Keywords - PTMi

Acetylation, Hypusine

Proteomic databases

EPDiP63241
MaxQBiP63241
PaxDbiP63241
PeptideAtlasiP63241
PRIDEiP63241
ProteomicsDBi57510
57511 [P63241-2]
TopDownProteomicsiP63241-1 [P63241-1]
P63241-2 [P63241-2]

2D gel databases

DOSAC-COBS-2DPAGEiP63241
OGPiP63241

PTM databases

iPTMnetiP63241
PhosphoSitePlusiP63241
SwissPalmiP63241

Miscellaneous databases

PMAP-CutDBiP63241

Expressioni

Tissue specificityi

Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000132507 Expressed in 218 organ(s), highest expression level in right testis
CleanExiHS_EIF5A
ExpressionAtlasiP63241 baseline and differential
GenevisibleiP63241 HS

Organism-specific databases

HPAiCAB005042
HPA061298

Interactioni

Subunit structurei

Interacts with DHPS, with SDCBP and DOHH. Interacts with HIV-1 protein Rev. Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4.5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi108299, 63 interactors
IntActiP63241, 40 interactors
MINTiP63241
STRINGi9606.ENSP00000336702

Structurei

Secondary structure

1154
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP63241
SMRiP63241
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63241

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 90DOHH-bindingAdd BLAST71

Sequence similaritiesi

Belongs to the eIF-5A family.Curated

Phylogenomic databases

eggNOGiKOG3271 Eukaryota
COG0231 LUCA
GeneTreeiENSGT00390000003738
HOVERGENiHBG001104
InParanoidiP63241
KOiK03263
OMAiDGYLCLM
PhylomeDBiP63241
TreeFamiTF101534

Family and domain databases

Gene3Di2.30.30.30, 1 hit
InterProiView protein in InterPro
IPR001884 IF5A-like
IPR012340 NA-bd_OB-fold
IPR014722 Rib_L2_dom2
IPR019769 Trans_elong_IF5A_hypusine_site
IPR020189 Transl_elong_IF5A_C
IPR008991 Translation_prot_SH3-like_sf
PANTHERiPTHR11673 PTHR11673, 1 hit
PfamiView protein in Pfam
PF01287 eIF-5a, 1 hit
PIRSFiPIRSF003025 eIF5A, 1 hit
SMARTiView protein in SMART
SM01376 eIF-5a, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00037 eIF_5A, 1 hit
PROSITEiView protein in PROSITE
PS00302 IF5A_HYPUSINE, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P63241-1) [UniParc]FASTAAdd to basket
Also known as: B, C, D

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK
60 70 80 90 100
HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS
110 120 130 140 150
LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK

AMAK
Length:154
Mass (Da):16,832
Last modified:January 23, 2007 - v2
Checksum:i07EF043C7DEA3091
GO
Isoform 2 (identifier: P63241-2) [UniParc]FASTAAdd to basket
Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MA → MCGTGGTDSKTRRPPHRASFLKRLESKPLKMA

Show »
Length:184
Mass (Da):20,170
Checksum:i9E2A400E66540B74
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
I3L397I3L397_HUMAN
Eukaryotic translation initiation f...
EIF5A
146Annotation score:
I3L504I3L504_HUMAN
Eukaryotic translation initiation f...
EIF5A
186Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36R → W in AAD14095 (PubMed:7545941).Curated1
Sequence conflicti45T → A in AAD14095 (PubMed:7545941).Curated1
Sequence conflicti85K → R in AAD14095 (PubMed:7545941).Curated1
Sequence conflicti109R → P in AAD14095 (PubMed:7545941).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0220201 – 2MA → MCGTGGTDSKTRRPPHRASF LKRLESKPLKMA in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23419 mRNA Translation: AAA58453.1
S72024 Genomic DNA Translation: AAD14095.1
U17969 Genomic DNA Translation: AAA86989.1
AY129319 mRNA Translation: AAN17514.1
AY129320 mRNA Translation: AAN17515.1
AY129321 mRNA Translation: AAN17516.1
AY129322 mRNA Translation: AAN17518.1
AK292615 mRNA Translation: BAF85304.1
CH471108 Genomic DNA Translation: EAW90219.1
CH471108 Genomic DNA Translation: EAW90220.1
CH471108 Genomic DNA Translation: EAW90221.1
CH471108 Genomic DNA Translation: EAW90222.1
BC000751 mRNA Translation: AAH00751.1
BC001832 mRNA Translation: AAH01832.1
BC030160 mRNA Translation: AAH30160.1
BC080196 mRNA Translation: AAH80196.1
BC085015 mRNA Translation: AAH85015.1
BC107779 mRNA Translation: AAI07780.1
CCDSiCCDS11099.1 [P63241-1]
CCDS45601.1 [P63241-2]
PIRiB31486 FIHUA
RefSeqiNP_001137232.1, NM_001143760.1 [P63241-2]
NP_001137233.1, NM_001143761.1 [P63241-1]
NP_001137234.1, NM_001143762.1 [P63241-1]
NP_001961.1, NM_001970.4 [P63241-1]
XP_005256566.1, XM_005256509.2 [P63241-1]
UniGeneiHs.104825
Hs.534314

Genome annotation databases

EnsembliENST00000336452; ENSP00000336702; ENSG00000132507 [P63241-2]
ENST00000336458; ENSP00000336776; ENSG00000132507 [P63241-1]
ENST00000416016; ENSP00000396073; ENSG00000132507 [P63241-1]
ENST00000419711; ENSP00000390677; ENSG00000132507 [P63241-1]
ENST00000571955; ENSP00000458269; ENSG00000132507 [P63241-1]
ENST00000573542; ENSP00000459611; ENSG00000132507 [P63241-1]
ENST00000576930; ENSP00000459196; ENSG00000132507 [P63241-1]
GeneIDi1984
KEGGihsa:1984
UCSCiuc002gfr.3 human [P63241-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23419 mRNA Translation: AAA58453.1
S72024 Genomic DNA Translation: AAD14095.1
U17969 Genomic DNA Translation: AAA86989.1
AY129319 mRNA Translation: AAN17514.1
AY129320 mRNA Translation: AAN17515.1
AY129321 mRNA Translation: AAN17516.1
AY129322 mRNA Translation: AAN17518.1
AK292615 mRNA Translation: BAF85304.1
CH471108 Genomic DNA Translation: EAW90219.1
CH471108 Genomic DNA Translation: EAW90220.1
CH471108 Genomic DNA Translation: EAW90221.1
CH471108 Genomic DNA Translation: EAW90222.1
BC000751 mRNA Translation: AAH00751.1
BC001832 mRNA Translation: AAH01832.1
BC030160 mRNA Translation: AAH30160.1
BC080196 mRNA Translation: AAH80196.1
BC085015 mRNA Translation: AAH85015.1
BC107779 mRNA Translation: AAI07780.1
CCDSiCCDS11099.1 [P63241-1]
CCDS45601.1 [P63241-2]
PIRiB31486 FIHUA
RefSeqiNP_001137232.1, NM_001143760.1 [P63241-2]
NP_001137233.1, NM_001143761.1 [P63241-1]
NP_001137234.1, NM_001143762.1 [P63241-1]
NP_001961.1, NM_001970.4 [P63241-1]
XP_005256566.1, XM_005256509.2 [P63241-1]
UniGeneiHs.104825
Hs.534314

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FH4model-A1-154[»]
3CPFX-ray2.50A/B15-151[»]
5DLQX-ray3.20E/F15-154[»]
ProteinModelPortaliP63241
SMRiP63241
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108299, 63 interactors
IntActiP63241, 40 interactors
MINTiP63241
STRINGi9606.ENSP00000336702

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

PTM databases

iPTMnetiP63241
PhosphoSitePlusiP63241
SwissPalmiP63241

Polymorphism and mutation databases

BioMutaiEIF5A
DMDMi54037409

2D gel databases

DOSAC-COBS-2DPAGEiP63241
OGPiP63241

Proteomic databases

EPDiP63241
MaxQBiP63241
PaxDbiP63241
PeptideAtlasiP63241
PRIDEiP63241
ProteomicsDBi57510
57511 [P63241-2]
TopDownProteomicsiP63241-1 [P63241-1]
P63241-2 [P63241-2]

Protocols and materials databases

DNASUi1984
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336452; ENSP00000336702; ENSG00000132507 [P63241-2]
ENST00000336458; ENSP00000336776; ENSG00000132507 [P63241-1]
ENST00000416016; ENSP00000396073; ENSG00000132507 [P63241-1]
ENST00000419711; ENSP00000390677; ENSG00000132507 [P63241-1]
ENST00000571955; ENSP00000458269; ENSG00000132507 [P63241-1]
ENST00000573542; ENSP00000459611; ENSG00000132507 [P63241-1]
ENST00000576930; ENSP00000459196; ENSG00000132507 [P63241-1]
GeneIDi1984
KEGGihsa:1984
UCSCiuc002gfr.3 human [P63241-1]

Organism-specific databases

CTDi1984
DisGeNETi1984
EuPathDBiHostDB:ENSG00000132507.17
GeneCardsiEIF5A
HGNCiHGNC:3300 EIF5A
HPAiCAB005042
HPA061298
MIMi600187 gene
neXtProtiNX_P63241
OpenTargetsiENSG00000132507
PharmGKBiPA27726
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3271 Eukaryota
COG0231 LUCA
GeneTreeiENSGT00390000003738
HOVERGENiHBG001104
InParanoidiP63241
KOiK03263
OMAiDGYLCLM
PhylomeDBiP63241
TreeFamiTF101534

Enzyme and pathway databases

ReactomeiR-HSA-204626 Hypusine synthesis from eIF5A-lysine

Miscellaneous databases

ChiTaRSiEIF5A human
EvolutionaryTraceiP63241
GeneWikiiEIF5A
GenomeRNAii1984
PMAP-CutDBiP63241
PROiPR:P63241
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132507 Expressed in 218 organ(s), highest expression level in right testis
CleanExiHS_EIF5A
ExpressionAtlasiP63241 baseline and differential
GenevisibleiP63241 HS

Family and domain databases

Gene3Di2.30.30.30, 1 hit
InterProiView protein in InterPro
IPR001884 IF5A-like
IPR012340 NA-bd_OB-fold
IPR014722 Rib_L2_dom2
IPR019769 Trans_elong_IF5A_hypusine_site
IPR020189 Transl_elong_IF5A_C
IPR008991 Translation_prot_SH3-like_sf
PANTHERiPTHR11673 PTHR11673, 1 hit
PfamiView protein in Pfam
PF01287 eIF-5a, 1 hit
PIRSFiPIRSF003025 eIF5A, 1 hit
SMARTiView protein in SMART
SM01376 eIF-5a, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00037 eIF_5A, 1 hit
PROSITEiView protein in PROSITE
PS00302 IF5A_HYPUSINE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIF5A1_HUMAN
AccessioniPrimary (citable) accession number: P63241
Secondary accession number(s): A8K9A0
, D3DTP2, P10159, Q16182, Q7L7L3, Q7Z4L1, Q9D0G2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Translation initiation factors
    List of translation initiation factor entries
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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