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UniProtKB - P63224 (GMHA_ECOLI)
Protein
Phosphoheptose isomerase
Gene
gmhA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.
2 PublicationsMiscellaneous
The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate.
Catalytic activityi
- 2 D-sedoheptulose 7-phosphate = D-glycero-α-D-manno-heptose 7-phosphate + D-glycero-β-D-manno-heptose 7-phosphate1 PublicationEC:5.3.1.281 Publication
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
: D-glycero-D-manno-heptose 7-phosphate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate. This subpathway is part of the pathway D-glycero-D-manno-heptose 7-phosphate biosynthesis, which is itself part of Carbohydrate biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate, the pathway D-glycero-D-manno-heptose 7-phosphate biosynthesis and in Carbohydrate biosynthesis.
Pathwayi: LPS core biosynthesis
This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 61 | ZincBy similarity | 1 | |
Binding sitei | 61 | Substrate | 1 | |
Metal bindingi | 65 | ZincBy similarity | 1 | |
Binding sitei | 65 | SubstrateBy similarity | 1 | |
Binding sitei | 120 | Substrate | 1 | |
Binding sitei | 124 | SubstrateBy similarity | 1 | |
Binding sitei | 169 | Substrate | 1 | |
Metal bindingi | 172 | ZincBy similarity | 1 | |
Binding sitei | 172 | Substrate | 1 | |
Metal bindingi | 180 | ZincBy similarity | 1 | |
Binding sitei | 180 | Substrate | 1 |
GO - Molecular functioni
- carbohydrate derivative binding Source: InterPro
- D-sedoheptulose 7-phosphate isomerase activity Source: EcoCyc
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- D-glycero-D-manno-heptose 7-phosphate biosynthetic process Source: EcoCyc
- lipopolysaccharide core region biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Isomerase |
Biological process | Carbohydrate metabolism, Lipopolysaccharide biosynthesis |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:G6106-MONOMER |
BRENDAi | 5.3.1.28, 2026 |
UniPathwayi | UPA00041;UER00436 UPA00958 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoheptose isomerase (EC:5.3.1.28)Alternative name(s): Sedoheptulose 7-phosphate isomerase |
Gene namesi | Name:gmhA Synonyms:lpcA, tfrA, yafI Ordered Locus Names:b0222, JW0212 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Other locations
- cytoplasm Source: EcoCyc
- protein-containing complex Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 61 | H → Q: Almost no effect. 1 Publication | 1 | |
Mutagenesisi | 65 | E → N or Q: No activity. 1 Publication | 1 | |
Mutagenesisi | 69 | R → Q: Almost no effect. 1 Publication | 1 | |
Mutagenesisi | 94 | D → N: No activity. 1 Publication | 1 | |
Mutagenesisi | 120 | T → A: No activity. 1 Publication | 1 | |
Mutagenesisi | 169 | D → N: No activity. 1 Publication | 1 | |
Mutagenesisi | 172 | Q → E: No activity. 1 Publication | 1 | |
Mutagenesisi | 180 | H → Q: No activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000136526 | 1 – 192 | Phosphoheptose isomeraseAdd BLAST | 192 |
Proteomic databases
jPOSTi | P63224 |
PaxDbi | P63224 |
PRIDEi | P63224 |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4263370, 277 interactors |
DIPi | DIP-48179N |
STRINGi | 511145.b0222 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P63224 |
SMRi | P63224 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P63224 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 37 – 192 | SISAdd BLAST | 156 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 54 – 55 | Substrate binding | 2 | |
Regioni | 93 – 94 | Substrate bindingBy similarity | 2 | |
Regioni | 119 – 121 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0279, Bacteria |
HOGENOMi | CLU_080999_4_0_6 |
InParanoidi | P63224 |
OMAi | FLAHKEA |
PhylomeDBi | P63224 |
Family and domain databases
CDDi | cd05006, SIS_GmhA, 1 hit |
HAMAPi | MF_00067, GmhA, 1 hit |
InterProi | View protein in InterPro IPR035461, GmhA/DiaA IPR004515, Phosphoheptose_Isoase IPR001347, SIS_dom IPR046348, SIS_dom_sf |
Pfami | View protein in Pfam PF13580, SIS_2, 1 hit |
SUPFAMi | SSF53697, SSF53697, 1 hit |
TIGRFAMsi | TIGR00441, gmhA, 1 hit |
PROSITEi | View protein in PROSITE PS51464, SIS, 1 hit |
i Sequence
Sequence statusi: Complete.
P63224-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYQDLIRNEL NEAAETLANF LKDDANIHAI QRAAVLLADS FKAGGKVLSC
60 70 80 90 100
GNGGSHCDAM HFAEELTGRY RENRPGYPAI AISDVSHISC VGNDFGFNDI
110 120 130 140 150
FSRYVEAVGR EGDVLLGIST SGNSANVIKA IAAAREKGMK VITLTGKDGG
160 170 180 190
KMAGTADIEI RVPHFGYADR IQEIHIKVIH ILIQLIEKEM VK
Sequence cautioni
The sequence AAB08644 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32590 Genomic DNA Translation: AAC43630.1 D38582 Genomic DNA Translation: BAA07584.1 U70214 Genomic DNA Translation: AAB08644.1 Different initiation. U00096 Genomic DNA Translation: AAC73326.1 AP009048 Genomic DNA Translation: BAA77892.1 |
PIRi | G64746 |
RefSeqi | NP_414757.1, NC_000913.3 WP_000284050.1, NZ_STEB01000020.1 |
Genome annotation databases
EnsemblBacteriai | AAC73326; AAC73326; b0222 BAA77892; BAA77892; BAA77892 |
GeneIDi | 66671459 949134 |
KEGGi | ecj:JW0212 eco:b0222 |
PATRICi | fig|1411691.4.peg.2061 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32590 Genomic DNA Translation: AAC43630.1 D38582 Genomic DNA Translation: BAA07584.1 U70214 Genomic DNA Translation: AAB08644.1 Different initiation. U00096 Genomic DNA Translation: AAC73326.1 AP009048 Genomic DNA Translation: BAA77892.1 |
PIRi | G64746 |
RefSeqi | NP_414757.1, NC_000913.3 WP_000284050.1, NZ_STEB01000020.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2I22 | X-ray | 2.80 | A/B/C/D | 1-192 | [»] | |
2I2W | X-ray | 1.95 | A/B/C/D | 1-192 | [»] | |
AlphaFoldDBi | P63224 | |||||
SMRi | P63224 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263370, 277 interactors |
DIPi | DIP-48179N |
STRINGi | 511145.b0222 |
Proteomic databases
jPOSTi | P63224 |
PaxDbi | P63224 |
PRIDEi | P63224 |
Genome annotation databases
EnsemblBacteriai | AAC73326; AAC73326; b0222 BAA77892; BAA77892; BAA77892 |
GeneIDi | 66671459 949134 |
KEGGi | ecj:JW0212 eco:b0222 |
PATRICi | fig|1411691.4.peg.2061 |
Organism-specific databases
EchoBASEi | EB2940 |
Phylogenomic databases
eggNOGi | COG0279, Bacteria |
HOGENOMi | CLU_080999_4_0_6 |
InParanoidi | P63224 |
OMAi | FLAHKEA |
PhylomeDBi | P63224 |
Enzyme and pathway databases
UniPathwayi | UPA00041;UER00436 UPA00958 |
BioCyci | EcoCyc:G6106-MONOMER |
BRENDAi | 5.3.1.28, 2026 |
Miscellaneous databases
EvolutionaryTracei | P63224 |
PROi | PR:P63224 |
Family and domain databases
CDDi | cd05006, SIS_GmhA, 1 hit |
HAMAPi | MF_00067, GmhA, 1 hit |
InterProi | View protein in InterPro IPR035461, GmhA/DiaA IPR004515, Phosphoheptose_Isoase IPR001347, SIS_dom IPR046348, SIS_dom_sf |
Pfami | View protein in Pfam PF13580, SIS_2, 1 hit |
SUPFAMi | SSF53697, SSF53697, 1 hit |
TIGRFAMsi | TIGR00441, gmhA, 1 hit |
PROSITEi | View protein in PROSITE PS51464, SIS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GMHA_ECOLI | |
Accessioni | P63224Primary (citable) accession number: P63224 Secondary accession number(s): P51001 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2004 |
Last sequence update: | September 27, 2004 | |
Last modified: | May 25, 2022 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families