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UniProtKB - P63177 (RLMB_ECOLI)

Entry version 129 (07 Oct 2020)
Sequence version 1 (27 Sep 2004)
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Protein

23S rRNA (guanosine-2'-O-)-methyltransferase RlmB

Gene

rlmB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Specifically methylates the ribose of guanosine 2251 in 23S rRNA.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei196S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei216S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei225S-adenosyl-L-methionine; via amide nitrogenBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • RNA binding Source: InterPro
  • RNA methyltransferase activity Source: GO_Central
  • rRNA (guanosine-2'-O-)-methyltransferase activity Source: EcoCyc
Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:G7845-MONOMER
MetaCyc:G7845-MONOMER

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.1.1.185, 2026

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (EC:2.1.1.185)
Alternative name(s):
23S rRNA (guanosine2251 2'-O)-methyltransferase
23S rRNA Gm2251 2'-O-methyltransferase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:rlmB
Synonyms:yjfH
Ordered Locus Names:b4180, JW4138
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Complete GO annotation on QuickGO ...

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001597851 – 24323S rRNA (guanosine-2'-O-)-methyltransferase RlmBAdd BLAST243

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P63177

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P63177

PRoteomics IDEntifications database

More...PRIDEi		P63177

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4261811, 37 interactors
852986, 2 interactors

Database of interacting proteins

More...DIPi		DIP-47866N

Protein interaction database and analysis system

More...IntActi		P63177, 19 interactors

STRING: functional protein association networks

More...STRINGi		511145.b4180

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1243
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P63177

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P63177

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. RlmB subfamily.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0566, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_021322_0_1_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P63177

KEGG Orthology (KO)

More...KOi		K03218

Database for complete collections of gene phylogenies

More...PhylomeDBi		P63177

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.30.1330.30, 1 hit
3.40.1280.10, 1 hit

HAMAP database of protein families

More...HAMAPi		MF_01887, 23SrRNA_methyltr_B, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR024915, 23S_rRNA_MeTrfase_RlmB
IPR029028, Alpha/beta_knot_MTases
IPR029064, L30e-like
IPR004441, rRNA_MeTrfase_TrmH
IPR001537, SpoU_MeTrfase
IPR013123, SpoU_subst-bd
IPR029026, tRNA_m1G_MTases_N

The PANTHER Classification System

More...PANTHERi		PTHR46429, PTHR46429, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00588, SpoU_methylase, 1 hit
PF08032, SpoU_sub_bind, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00967, SpoU_sub_bind, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF55315, SSF55315, 1 hit
SSF75217, SSF75217, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00186, rRNA_methyl_3, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P63177-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEMIYGIHA VQALLERAPE RFQEVFILKG REDKRLLPLI HALESQGVVI 
        60         70         80         90        100
QLANRQYLDE KSDGAVHQGI IARVKPGRQY QENDLPDLIA SLDQPFLLIL 
       110        120        130        140        150
DGVTDPHNLG ACLRSADAAG VHAVIVPKDR SAQLNATAKK VACGAAESVP 
       160        170        180        190        200
LIRVTNLART MRMLQEENIW IVGTAGEADH TLYQSKMTGR LALVMGAEGE 
       210        220        230        240 
GMRRLTREHC DELISIPMAG SVSSLNVSVA TGICLFEAVR QRS
Length:243
Mass (Da):26,557
Last modified:September 27, 2004 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i6D6C4170580341D6
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97076.1
U00096 Genomic DNA Translation: AAC77137.1
AP009048 Genomic DNA Translation: BAE78181.1

Protein sequence database of the Protein Information Resource

More...PIRi		S56405

NCBI Reference Sequences

More...RefSeqi		NP_418601.1, NC_000913.3
WP_001293282.1, NZ_STEB01000013.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC77137; AAC77137; b4180
BAE78181; BAE78181; BAE78181

Database of genes from NCBI RefSeq genomes

More...GeneIDi		52078539
948694

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW4138
eco:b4180

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.2521

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97076.1
U00096 Genomic DNA Translation: AAC77137.1
AP009048 Genomic DNA Translation: BAE78181.1
PIRiS56405
RefSeqiNP_418601.1, NC_000913.3
WP_001293282.1, NZ_STEB01000013.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GZ0X-ray2.50A/B/C/D/E/F/G/H1-243[»]
SMRiP63177
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi4261811, 37 interactors
852986, 2 interactors
DIPiDIP-47866N
IntActiP63177, 19 interactors
STRINGi511145.b4180

Proteomic databases

jPOSTiP63177
PaxDbiP63177
PRIDEiP63177

Genome annotation databases

EnsemblBacteriaiAAC77137; AAC77137; b4180
BAE78181; BAE78181; BAE78181
GeneIDi52078539
948694
KEGGiecj:JW4138
eco:b4180
PATRICifig|1411691.4.peg.2521

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB2376

Phylogenomic databases

eggNOGiCOG0566, Bacteria
HOGENOMiCLU_021322_0_1_6
InParanoidiP63177
KOiK03218
PhylomeDBiP63177

Enzyme and pathway databases

BioCyciEcoCyc:G7845-MONOMER
MetaCyc:G7845-MONOMER
BRENDAi2.1.1.185, 2026

Miscellaneous databases

EvolutionaryTraceiP63177

Protein Ontology

More...PROi		PR:P63177

Family and domain databases

Gene3Di3.30.1330.30, 1 hit
3.40.1280.10, 1 hit
HAMAPiMF_01887, 23SrRNA_methyltr_B, 1 hit
InterProiView protein in InterPro
IPR024915, 23S_rRNA_MeTrfase_RlmB
IPR029028, Alpha/beta_knot_MTases
IPR029064, L30e-like
IPR004441, rRNA_MeTrfase_TrmH
IPR001537, SpoU_MeTrfase
IPR013123, SpoU_subst-bd
IPR029026, tRNA_m1G_MTases_N
PANTHERiPTHR46429, PTHR46429, 1 hit
PfamiView protein in Pfam
PF00588, SpoU_methylase, 1 hit
PF08032, SpoU_sub_bind, 1 hit
SMARTiView protein in SMART
SM00967, SpoU_sub_bind, 1 hit
SUPFAMiSSF55315, SSF55315, 1 hit
SSF75217, SSF75217, 1 hit
TIGRFAMsiTIGR00186, rRNA_methyl_3, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRLMB_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P63177
Secondary accession number(s): P39290, Q2M6C5
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: October 7, 2020
This is version 129 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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