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UniProtKB - P63177 (RLMB_ECOLI)
Protein
23S rRNA (guanosine-2'-O-)-methyltransferase RlmB
Gene
rlmB
Organism
Escherichia coli (strain K12)
Status
Functioni
Specifically methylates the ribose of guanosine 2251 in 23S rRNA.
1 PublicationCatalytic activityi
- guanosine2251 in 23S rRNA + S-adenosyl-L-methionine = 2'-O-methylguanosine2251 in 23S rRNA + H+ + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.1851 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 196 | S-adenosyl-L-methionine; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 216 | S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 225 | S-adenosyl-L-methionine; via amide nitrogenBy similarity | 1 |
GO - Molecular functioni
- RNA binding Source: InterPro
- RNA methyltransferase activity Source: GO_Central
- rRNA (guanosine-2'-O-)-methyltransferase activity Source: EcoCyc
GO - Biological processi
- enzyme-directed rRNA 2'-O-methylation Source: EcoCyc
- RNA methylation Source: GO_Central
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | rRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:G7845-MONOMER |
BRENDAi | 2.1.1.185, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (EC:2.1.1.185)Alternative name(s): 23S rRNA (guanosine2251 2'-O)-methyltransferase 23S rRNA Gm2251 2'-O-methyltransferase |
Gene namesi | Name:rlmB Synonyms:yjfH Ordered Locus Names:b4180, JW4138 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000159785 | 1 – 243 | 23S rRNA (guanosine-2'-O-)-methyltransferase RlmBAdd BLAST | 243 |
Proteomic databases
jPOSTi | P63177 |
PaxDbi | P63177 |
PRIDEi | P63177 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 4261811, 37 interactors 852986, 2 interactors |
DIPi | DIP-47866N |
IntActi | P63177, 19 interactors |
STRINGi | 511145.b4180 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P63177 |
SMRi | P63177 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P63177 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. RlmB subfamily.Curated
Phylogenomic databases
eggNOGi | COG0566, Bacteria |
HOGENOMi | CLU_021322_0_1_6 |
InParanoidi | P63177 |
OMAi | QVPPYEY |
PhylomeDBi | P63177 |
Family and domain databases
Gene3Di | 3.30.1330.30, 1 hit 3.40.1280.10, 1 hit |
HAMAPi | MF_01887, 23SrRNA_methyltr_B, 1 hit |
InterProi | View protein in InterPro IPR024915, 23S_rRNA_MeTrfase_RlmB IPR029028, Alpha/beta_knot_MTases IPR029064, L30e-like IPR004441, rRNA_MeTrfase_TrmH IPR001537, SpoU_MeTrfase IPR013123, SpoU_subst-bd IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR46429, PTHR46429, 1 hit |
Pfami | View protein in Pfam PF00588, SpoU_methylase, 1 hit PF08032, SpoU_sub_bind, 1 hit |
SMARTi | View protein in SMART SM00967, SpoU_sub_bind, 1 hit |
SUPFAMi | SSF55315, SSF55315, 1 hit SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00186, rRNA_methyl_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P63177-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEMIYGIHA VQALLERAPE RFQEVFILKG REDKRLLPLI HALESQGVVI
60 70 80 90 100
QLANRQYLDE KSDGAVHQGI IARVKPGRQY QENDLPDLIA SLDQPFLLIL
110 120 130 140 150
DGVTDPHNLG ACLRSADAAG VHAVIVPKDR SAQLNATAKK VACGAAESVP
160 170 180 190 200
LIRVTNLART MRMLQEENIW IVGTAGEADH TLYQSKMTGR LALVMGAEGE
210 220 230 240
GMRRLTREHC DELISIPMAG SVSSLNVSVA TGICLFEAVR QRS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97076.1 U00096 Genomic DNA Translation: AAC77137.1 AP009048 Genomic DNA Translation: BAE78181.1 |
PIRi | S56405 |
RefSeqi | NP_418601.1, NC_000913.3 WP_001293282.1, NZ_STEB01000013.1 |
Genome annotation databases
EnsemblBacteriai | AAC77137; AAC77137; b4180 BAE78181; BAE78181; BAE78181 |
GeneIDi | 67414798 948694 |
KEGGi | ecj:JW4138 eco:b4180 |
PATRICi | fig|1411691.4.peg.2521 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97076.1 U00096 Genomic DNA Translation: AAC77137.1 AP009048 Genomic DNA Translation: BAE78181.1 |
PIRi | S56405 |
RefSeqi | NP_418601.1, NC_000913.3 WP_001293282.1, NZ_STEB01000013.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GZ0 | X-ray | 2.50 | A/B/C/D/E/F/G/H | 1-243 | [»] | |
AlphaFoldDBi | P63177 | |||||
SMRi | P63177 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261811, 37 interactors 852986, 2 interactors |
DIPi | DIP-47866N |
IntActi | P63177, 19 interactors |
STRINGi | 511145.b4180 |
Proteomic databases
jPOSTi | P63177 |
PaxDbi | P63177 |
PRIDEi | P63177 |
Genome annotation databases
EnsemblBacteriai | AAC77137; AAC77137; b4180 BAE78181; BAE78181; BAE78181 |
GeneIDi | 67414798 948694 |
KEGGi | ecj:JW4138 eco:b4180 |
PATRICi | fig|1411691.4.peg.2521 |
Organism-specific databases
EchoBASEi | EB2376 |
Phylogenomic databases
eggNOGi | COG0566, Bacteria |
HOGENOMi | CLU_021322_0_1_6 |
InParanoidi | P63177 |
OMAi | QVPPYEY |
PhylomeDBi | P63177 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7845-MONOMER |
BRENDAi | 2.1.1.185, 2026 |
Miscellaneous databases
EvolutionaryTracei | P63177 |
PROi | PR:P63177 |
Family and domain databases
Gene3Di | 3.30.1330.30, 1 hit 3.40.1280.10, 1 hit |
HAMAPi | MF_01887, 23SrRNA_methyltr_B, 1 hit |
InterProi | View protein in InterPro IPR024915, 23S_rRNA_MeTrfase_RlmB IPR029028, Alpha/beta_knot_MTases IPR029064, L30e-like IPR004441, rRNA_MeTrfase_TrmH IPR001537, SpoU_MeTrfase IPR013123, SpoU_subst-bd IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR46429, PTHR46429, 1 hit |
Pfami | View protein in Pfam PF00588, SpoU_methylase, 1 hit PF08032, SpoU_sub_bind, 1 hit |
SMARTi | View protein in SMART SM00967, SpoU_sub_bind, 1 hit |
SUPFAMi | SSF55315, SSF55315, 1 hit SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00186, rRNA_methyl_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RLMB_ECOLI | |
Accessioni | P63177Primary (citable) accession number: P63177 Secondary accession number(s): P39290, Q2M6C5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2004 |
Last sequence update: | September 27, 2004 | |
Last modified: | May 25, 2022 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families