UniProtKB - P63168 (DYL1_MOUSE)
Dynein light chain 1, cytoplasmic
Dynll1
Functioni
Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures (By similarity).
By similarityBinds and inhibits the catalytic activity of neuronal nitric oxide synthase.
By similarityPromotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1.
By similarityRegulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity (By similarity).
By similarityGO - Molecular functioni
- dynein intermediate chain binding Source: MGI
- dynein light intermediate chain binding Source: GO_Central
- enzyme binding Source: MGI
- enzyme inhibitor activity Source: MGI
- identical protein binding Source: MGI
- nitric-oxide synthase regulator activity Source: MGI
- protein-containing complex binding Source: MGI
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- scaffold protein binding Source: MGI
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cilium assembly Source: GO_Central
- intraciliary retrograde transport Source: MGI
- motile cilium assembly Source: MGI
- negative regulation of nitric oxide biosynthetic process Source: MGI
- negative regulation of phosphorylation Source: MGI
- positive regulation of non-motile cilium assembly Source: MGI
Keywordsi
Molecular function | Activator, Motor protein |
Biological process | Apoptosis, Transcription, Transcription regulation, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-111446, Activation of BIM and translocation to mitochondria R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-1632852, Macroautophagy R-MMU-2132295, MHC class II antigen presentation R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-3371497, HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-5620924, Intraflagellar transport R-MMU-5663220, RHO GTPases Activate Formins R-MMU-6798695, Neutrophil degranulation R-MMU-6807878, COPI-mediated anterograde transport R-MMU-6811436, COPI-independent Golgi-to-ER retrograde traffic R-MMU-68877, Mitotic Prometaphase R-MMU-8854518, AURKA Activation by TPX2 R-MMU-9646399, Aggrephagy R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Names & Taxonomyi
Protein namesi | Recommended name: Dynein light chain 1, cytoplasmicAlternative name(s): 8 kDa dynein light chain Short name: DLC8 Dynein light chain LC8-type 1 Protein inhibitor of neuronal nitric oxide synthase Short name: PIN Short name: mPIN |
Gene namesi | Name:Dynll1 Synonyms:Dlc1, Dncl1, Dnclc1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1861457, Dynll1 |
VEuPathDBi | HostDB:ENSMUSG00000009013 |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
- cytoskeleton By similarity
Mitochondrion
- Mitochondrion By similarity
Nucleus
- Nucleus By similarity
Note: Upon induction of apoptosis translocates together with BCL2L11 to mitochondria.By similarity
Cytoskeleton
- centrosome Source: MGI
- cytoplasmic dynein complex Source: MGI
- cytoskeleton Source: MGI
- dynein complex Source: UniProtKB
- microtubule Source: UniProtKB-KW
- mitotic spindle Source: UniProtKB
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: UniProtKB-SubCell
Nucleus
- nucleus Source: MGI
Other locations
- axon cytoplasm Source: MGI
- cilium Source: GOC
- cytoplasm Source: MGI
- kinetochore Source: UniProtKB
- membrane Source: MGI
- secretory granule Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Dynein, Microtubule, Mitochondrion, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000195127 | 1 – 89 | Dynein light chain 1, cytoplasmicAdd BLAST | 89 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 36 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 43 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 88 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P63168 |
jPOSTi | P63168 |
MaxQBi | P63168 |
PaxDbi | P63168 |
PeptideAtlasi | P63168 |
PRIDEi | P63168 |
ProteomicsDBi | 277617 |
TopDownProteomicsi | P63168 |
PTM databases
iPTMneti | P63168 |
PhosphoSitePlusi | P63168 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000009013, Expressed in testis and 305 other tissues |
Genevisiblei | P63168, MM |
Interactioni
Subunit structurei
Homodimer. Monomer; the monomeric form is incapable of binding to target proteins. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer.
Interacts with TXNDC17.
Interacts with WWC1 and ESR1. The interaction with WWC1 is mandatory for the recruitment and transactivation functions of ESR1 or DYNLL1 to the target chromatin.
Interacts with BCL2; the interaction is greatly enhanced in the nucleus and in mitochondria upon induction of apoptosis.
Interacts with PAK1; the interaction requires dimeric DYNLL1.
Interacts with MYZAP.
Part of an astrin (SPAG5)-kinastrin (SKAP) complex containing KNSTRN, SPAG5, PLK1, DYNLL1 and SGO2.
Interacts with ATMIN; this interaction inhibits ATMIN transcriptional activity and hence may play a role in a feedback loop whereby DYNLL1 inhibits transactivation of its own promoter by ATMIN.
Interacts with NEK9 (not phosphorylated at 'Ser-944') (By similarity).
Interacts with BCL2L11 (PubMed:21478148).
Interacts with BICD2 (PubMed:22956769).
Interacts with BCAS1 (By similarity).
Interacts with Bassoon/BSN (By similarity).
Interacts with HDAC6 (By similarity).
Interacts with TPPP (By similarity).
Interacts with AMBRA1 (via TQT motifs); tethering AMBRA1 to the cytoskeleton (By similarity).
Interacts with FAM83D/CHICA (via C-terminus) (By similarity).
Interacts with HMMR, SPAG5/Astrin and KNSTRN/Kinastrin (By similarity).
By similarity2 PublicationsBinary interactionsi
P63168
With | #Exp. | IntAct |
---|---|---|
Dazl [Q64368] | 12 | EBI-349121,EBI-2024439 |
Dync1i1 [O88485] | 2 | EBI-349121,EBI-492834 |
PAX6 [P26367] from Homo sapiens. | 3 | EBI-349121,EBI-747278 |
GO - Molecular functioni
- dynein intermediate chain binding Source: MGI
- dynein light intermediate chain binding Source: GO_Central
- enzyme binding Source: MGI
- identical protein binding Source: MGI
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- scaffold protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 207994, 111 interactors |
ComplexPortali | CPX-5699, Cytoplasmic dynein complex, variant 1 |
ELMi | P63168 |
IntActi | P63168, 74 interactors |
MINTi | P63168 |
STRINGi | 10090.ENSMUSP00000009157 |
Miscellaneous databases
RNActi | P63168, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 67 – 89 | Interaction with ESR1By similarityAdd BLAST | 23 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3430, Eukaryota |
GeneTreei | ENSGT00390000000378 |
HOGENOMi | CLU_070944_4_0_1 |
InParanoidi | P63168 |
OMAi | FVTHEKH |
OrthoDBi | 1520814at2759 |
PhylomeDBi | P63168 |
TreeFami | TF300264 |
Family and domain databases
Gene3Di | 3.30.740.10, 1 hit |
InterProi | View protein in InterPro IPR037177, DLC_sf IPR019763, Dynein_light_1/2_CS IPR001372, Dynein_light_chain_typ-1/2 |
PANTHERi | PTHR11886, PTHR11886, 1 hit |
Pfami | View protein in Pfam PF01221, Dynein_light, 1 hit |
SMARTi | View protein in SMART SM01375, Dynein_light, 1 hit |
SUPFAMi | SSF54648, SSF54648, 1 hit |
PROSITEi | View protein in PROSITE PS01239, DYNEIN_LIGHT_1, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY
60 70 80
NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF020185 mRNA Translation: AAD01643.1 AK002522 mRNA Translation: BAB22160.1 AK010614 mRNA Translation: BAB27063.1 AK010685 mRNA Translation: BAB27117.1 AK013721 mRNA Translation: BAB28970.1 AK082923 mRNA Translation: BAC38691.1 AK147977 mRNA Translation: BAE28262.1 BC008106 mRNA Translation: AAH08106.1 BC034258 mRNA Translation: AAH34258.1 |
CCDSi | CCDS39227.1 |
RefSeqi | NP_062656.3, NM_019682.4 |
Genome annotation databases
Ensembli | ENSMUST00000009157; ENSMUSP00000009157; ENSMUSG00000009013 ENSMUST00000112090; ENSMUSP00000107720; ENSMUSG00000009013 |
GeneIDi | 56455 |
KEGGi | mmu:56455 |
UCSCi | uc008zdp.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF020185 mRNA Translation: AAD01643.1 AK002522 mRNA Translation: BAB22160.1 AK010614 mRNA Translation: BAB27063.1 AK010685 mRNA Translation: BAB27117.1 AK013721 mRNA Translation: BAB28970.1 AK082923 mRNA Translation: BAC38691.1 AK147977 mRNA Translation: BAE28262.1 BC008106 mRNA Translation: AAH08106.1 BC034258 mRNA Translation: AAH34258.1 |
CCDSi | CCDS39227.1 |
RefSeqi | NP_062656.3, NM_019682.4 |
3D structure databases
BMRBi | P63168 |
SMRi | P63168 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207994, 111 interactors |
ComplexPortali | CPX-5699, Cytoplasmic dynein complex, variant 1 |
ELMi | P63168 |
IntActi | P63168, 74 interactors |
MINTi | P63168 |
STRINGi | 10090.ENSMUSP00000009157 |
PTM databases
iPTMneti | P63168 |
PhosphoSitePlusi | P63168 |
Proteomic databases
EPDi | P63168 |
jPOSTi | P63168 |
MaxQBi | P63168 |
PaxDbi | P63168 |
PeptideAtlasi | P63168 |
PRIDEi | P63168 |
ProteomicsDBi | 277617 |
TopDownProteomicsi | P63168 |
Protocols and materials databases
DNASUi | 56455 |
Genome annotation databases
Ensembli | ENSMUST00000009157; ENSMUSP00000009157; ENSMUSG00000009013 ENSMUST00000112090; ENSMUSP00000107720; ENSMUSG00000009013 |
GeneIDi | 56455 |
KEGGi | mmu:56455 |
UCSCi | uc008zdp.2, mouse |
Organism-specific databases
CTDi | 8655 |
MGIi | MGI:1861457, Dynll1 |
VEuPathDBi | HostDB:ENSMUSG00000009013 |
Phylogenomic databases
eggNOGi | KOG3430, Eukaryota |
GeneTreei | ENSGT00390000000378 |
HOGENOMi | CLU_070944_4_0_1 |
InParanoidi | P63168 |
OMAi | FVTHEKH |
OrthoDBi | 1520814at2759 |
PhylomeDBi | P63168 |
TreeFami | TF300264 |
Enzyme and pathway databases
Reactomei | R-MMU-111446, Activation of BIM and translocation to mitochondria R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-1632852, Macroautophagy R-MMU-2132295, MHC class II antigen presentation R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-3371497, HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-5620924, Intraflagellar transport R-MMU-5663220, RHO GTPases Activate Formins R-MMU-6798695, Neutrophil degranulation R-MMU-6807878, COPI-mediated anterograde transport R-MMU-6811436, COPI-independent Golgi-to-ER retrograde traffic R-MMU-68877, Mitotic Prometaphase R-MMU-8854518, AURKA Activation by TPX2 R-MMU-9646399, Aggrephagy R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Miscellaneous databases
BioGRID-ORCSi | 56455, 8 hits in 32 CRISPR screens |
ChiTaRSi | Dynll1, mouse |
PROi | PR:P63168 |
RNActi | P63168, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000009013, Expressed in testis and 305 other tissues |
Genevisiblei | P63168, MM |
Family and domain databases
Gene3Di | 3.30.740.10, 1 hit |
InterProi | View protein in InterPro IPR037177, DLC_sf IPR019763, Dynein_light_1/2_CS IPR001372, Dynein_light_chain_typ-1/2 |
PANTHERi | PTHR11886, PTHR11886, 1 hit |
Pfami | View protein in Pfam PF01221, Dynein_light, 1 hit |
SMARTi | View protein in SMART SM01375, Dynein_light, 1 hit |
SUPFAMi | SSF54648, SSF54648, 1 hit |
PROSITEi | View protein in PROSITE PS01239, DYNEIN_LIGHT_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DYL1_MOUSE | |
Accessioni | P63168Primary (citable) accession number: P63168 Secondary accession number(s): Q15701, Q3UGE7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2004 |
Last sequence update: | September 27, 2004 | |
Last modified: | February 23, 2022 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families