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Protein

Dynein light chain 1, cytoplasmic

Gene

DYNLL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.
Binds and inhibits the catalytic activity of neuronal nitric oxide synthase.
Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1.
Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Motor protein
Biological processApoptosis, Host-virus interaction, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-111446 Activation of BIM and translocation to mitochondria
R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-1632852 Macroautophagy
R-HSA-2132295 MHC class II antigen presentation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-HSA-380259 Loss of Nlp from mitotic centrosomes
R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes
R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome
R-HSA-380320 Recruitment of NuMA to mitotic centrosomes
R-HSA-5620912 Anchoring of the basal body to the plasma membrane
R-HSA-5620924 Intraflagellar transport
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-6798695 Neutrophil degranulation
R-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-HSA-68877 Mitotic Prometaphase
R-HSA-8854518 AURKA Activation by TPX2
SIGNORiP63167

Names & Taxonomyi

Protein namesi
Recommended name:
Dynein light chain 1, cytoplasmic
Alternative name(s):
8 kDa dynein light chain
Short name:
DLC8
Dynein light chain LC8-type 1
Protein inhibitor of neuronal nitric oxide synthase
Short name:
PIN
Gene namesi
Name:DYNLL1
Synonyms:DLC1, DNCL1, DNCLC1, HDLC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000088986.10
HGNCiHGNC:15476 DYNLL1
MIMi601562 gene
neXtProtiNX_P63167

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule, Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67T → A: Abolishes interaction with PAK1. 1 Publication1
Mutagenesisi88S → A: Abolishes growth factor-mediated phosphorylation. Increases BCL2L11 protein level and promotes apoptosis. 2 Publications1
Mutagenesisi88S → E: Abolishes homodimerization. Abolishes interaction with BCL2L11 isoform 1 and isoform 2. 2 Publications1

Organism-specific databases

DisGeNETi8655
OpenTargetsiENSG00000088986
PharmGKBiPA134972261

Polymorphism and mutation databases

BioMutaiDYNLL1
DMDMi52783578

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001951251 – 89Dynein light chain 1, cytoplasmicAdd BLAST89

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36N6-acetyllysineCombined sources1
Cross-linki43Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei88Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylation at Ser-88 appears to control the dimer-monomer transition. According to PubMed:15193260, it is phosphorylated at Ser-88 by PAK1, however, according to PubMed:18650427, the DYNLL1 dimer is not accessible for PAK1 and the phosphorylation could not be demonstrated in vitro.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP63167
PaxDbiP63167
PeptideAtlasiP63167
PRIDEiP63167
ProteomicsDBi57501
TopDownProteomicsiP63167

PTM databases

iPTMnetiP63167
PhosphoSitePlusiP63167
SwissPalmiP63167

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Inductioni

Up-regulated by ATMIN, PAK1 and estrogen.2 Publications

Gene expression databases

BgeeiENSG00000088986 Expressed in 238 organ(s), highest expression level in anterior cingulate cortex
CleanExiHS_DLC1
HS_DYNLL1
ExpressionAtlasiP63167 baseline and differential
GenevisibleiP63167 HS

Organism-specific databases

HPAiHPA039954

Interactioni

Subunit structurei

Homodimer. Monomer; the monomeric form is incapable of binding to target proteins. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with TXNDC17. Interacts with WWC1 and ESR1. The WWC1-DYNLL1 interaction is mandatory for the recruitment and transactivation functions of ESR1 or DYNLL1 to the target chromatin. Interacts with BCL2L11 isoform 1 and isoform 2. Interacts with BCL2; the interaction is greatly enhanced in the nucleus and in mitochondria upon induction of apoptosis. Interacts with PAK1; the interaction requires dimeric DYNLL1. Interacts with MYZAP. Part of an astrin (SPAG5)-kinastrin (SKAP) complex containing KNSTRN, SPAG5, PLK1, DYNLL1 and SGO2. Interacts with ATMIN; this interaction inhibits ATMIN transcriptional activity and hence may play a role in a feedback loop whereby DYNLL1 inhibits transactivation of its own promoter by ATMIN. Interacts with NEK9 (not phosphorylated at 'Ser-944'). Interacts with BICD2 (By similarity). Interacts with BCAS1 (By similarity).By similarity11 Publications
(Microbial infection) Interacts with human spumaretrovirus Gag protein; this interaction is critical for intracellular microtubule-dependent viral genome transport toward the centrosome.1 Publication
(Microbial infection) Interacts with ebolavirus protein VP35; this interaction stabilizes VP35 N-terminal oligomerization domain and enhances viral RNA synthesis.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114206, 231 interactors
CORUMiP63167
DIPiDIP-33150N
IntActiP63167, 194 interactors
MINTiP63167
STRINGi9606.ENSP00000242577

Structurei

Secondary structure

189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP63167
SMRiP63167
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63167

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 89Interaction with ESR11 PublicationAdd BLAST23

Sequence similaritiesi

Belongs to the dynein light chain family.Curated

Phylogenomic databases

eggNOGiKOG3430 Eukaryota
ENOG4111NK2 LUCA
GeneTreeiENSGT00390000000378
HOVERGENiHBG002133
InParanoidiP63167
KOiK10418
OMAiQAIMSDR
OrthoDBiEOG091G15JY
PhylomeDBiP63167
TreeFamiTF300264

Family and domain databases

Gene3Di3.30.740.10, 1 hit
InterProiView protein in InterPro
IPR037177 DLC_sf
IPR019763 Dynein_light_1/2_CS
IPR001372 Dynein_light_chain_typ-1/2
PANTHERiPTHR11886 PTHR11886, 1 hit
PfamiView protein in Pfam
PF01221 Dynein_light, 1 hit
SMARTiView protein in SMART
SM01375 Dynein_light, 1 hit
SUPFAMiSSF54648 SSF54648, 1 hit
PROSITEiView protein in PROSITE
PS01239 DYNEIN_LIGHT_1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P63167-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY
60 70 80
NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG
Length:89
Mass (Da):10,366
Last modified:September 27, 2004 - v1
Checksum:iF5E7647D092BEB3A
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VXL2F8VXL2_HUMAN
Dynein light chain
DYNLL1
47Annotation score:
F8VXI7F8VXI7_HUMAN
Dynein light chain
DYNLL1
42Annotation score:
F8VRV5F8VRV5_HUMAN
Dynein light chain
DYNLL1
67Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32944 mRNA Translation: AAB04149.1
CR407672 mRNA Translation: CAG28600.1
CCDSiCCDS9200.1
RefSeqiNP_001032583.1, NM_001037494.1
NP_001032584.1, NM_001037495.1
NP_003737.1, NM_003746.2
UniGeneiHs.5120

Genome annotation databases

EnsembliENST00000242577; ENSP00000242577; ENSG00000088986
ENST00000392508; ENSP00000376296; ENSG00000088986
ENST00000392509; ENSP00000376297; ENSG00000088986
ENST00000548342; ENSP00000447907; ENSG00000088986
ENST00000549989; ENSP00000446614; ENSG00000088986
GeneIDi8655
KEGGihsa:8655

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32944 mRNA Translation: AAB04149.1
CR407672 mRNA Translation: CAG28600.1
CCDSiCCDS9200.1
RefSeqiNP_001032583.1, NM_001037494.1
NP_001032584.1, NM_001037495.1
NP_003737.1, NM_003746.2
UniGeneiHs.5120

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CMIX-ray2.50A/B5-89[»]
3ZKEX-ray2.20A/C/E/G/I/K1-89[»]
3ZKFX-ray2.60A/C/E/G/I/K1-89[»]
ProteinModelPortaliP63167
SMRiP63167
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114206, 231 interactors
CORUMiP63167
DIPiDIP-33150N
IntActiP63167, 194 interactors
MINTiP63167
STRINGi9606.ENSP00000242577

PTM databases

iPTMnetiP63167
PhosphoSitePlusiP63167
SwissPalmiP63167

Polymorphism and mutation databases

BioMutaiDYNLL1
DMDMi52783578

Proteomic databases

EPDiP63167
PaxDbiP63167
PeptideAtlasiP63167
PRIDEiP63167
ProteomicsDBi57501
TopDownProteomicsiP63167

Protocols and materials databases

DNASUi8655
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000242577; ENSP00000242577; ENSG00000088986
ENST00000392508; ENSP00000376296; ENSG00000088986
ENST00000392509; ENSP00000376297; ENSG00000088986
ENST00000548342; ENSP00000447907; ENSG00000088986
ENST00000549989; ENSP00000446614; ENSG00000088986
GeneIDi8655
KEGGihsa:8655

Organism-specific databases

CTDi8655
DisGeNETi8655
EuPathDBiHostDB:ENSG00000088986.10
GeneCardsiDYNLL1
HGNCiHGNC:15476 DYNLL1
HPAiHPA039954
MIMi601562 gene
neXtProtiNX_P63167
OpenTargetsiENSG00000088986
PharmGKBiPA134972261
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3430 Eukaryota
ENOG4111NK2 LUCA
GeneTreeiENSGT00390000000378
HOVERGENiHBG002133
InParanoidiP63167
KOiK10418
OMAiQAIMSDR
OrthoDBiEOG091G15JY
PhylomeDBiP63167
TreeFamiTF300264

Enzyme and pathway databases

ReactomeiR-HSA-111446 Activation of BIM and translocation to mitochondria
R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-1632852 Macroautophagy
R-HSA-2132295 MHC class II antigen presentation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-HSA-380259 Loss of Nlp from mitotic centrosomes
R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes
R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome
R-HSA-380320 Recruitment of NuMA to mitotic centrosomes
R-HSA-5620912 Anchoring of the basal body to the plasma membrane
R-HSA-5620924 Intraflagellar transport
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-6798695 Neutrophil degranulation
R-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-HSA-68877 Mitotic Prometaphase
R-HSA-8854518 AURKA Activation by TPX2
SIGNORiP63167

Miscellaneous databases

ChiTaRSiDYNLL1 human
EvolutionaryTraceiP63167
GeneWikiiDYNLL1
GenomeRNAii8655
PROiPR:P63167
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000088986 Expressed in 238 organ(s), highest expression level in anterior cingulate cortex
CleanExiHS_DLC1
HS_DYNLL1
ExpressionAtlasiP63167 baseline and differential
GenevisibleiP63167 HS

Family and domain databases

Gene3Di3.30.740.10, 1 hit
InterProiView protein in InterPro
IPR037177 DLC_sf
IPR019763 Dynein_light_1/2_CS
IPR001372 Dynein_light_chain_typ-1/2
PANTHERiPTHR11886 PTHR11886, 1 hit
PfamiView protein in Pfam
PF01221 Dynein_light, 1 hit
SMARTiView protein in SMART
SM01375 Dynein_light, 1 hit
SUPFAMiSSF54648 SSF54648, 1 hit
PROSITEiView protein in PROSITE
PS01239 DYNEIN_LIGHT_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDYL1_HUMAN
AccessioniPrimary (citable) accession number: P63167
Secondary accession number(s): Q15701
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: November 7, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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