UniProtKB - P63166 (SUMO1_MOUSE)
Small ubiquitin-related modifier 1
Sumo1
Functioni
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (By similarity).
By similarity1 PublicationGO - Molecular functioni
- enzyme binding Source: MGI
- potassium channel regulator activity Source: UniProtKB
- protein tag Source: GO_Central
- small protein activating enzyme binding Source: MGI
- transcription factor binding Source: AgBase
- ubiquitin-like protein ligase binding Source: GO_Central
- ubiquitin protein ligase binding Source: UniProtKB
- ubiquitin-specific protease binding Source: MGI
GO - Biological processi
- cellular response to cadmium ion Source: UniProtKB
- cellular response to heat Source: UniProtKB
- negative regulation of action potential Source: UniProtKB
- negative regulation of delayed rectifier potassium channel activity Source: UniProtKB
- negative regulation of DNA binding Source: MGI
- negative regulation of DNA-binding transcription factor activity Source: MGI
- negative regulation of protein import into nucleus Source: MGI
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by transcription factor localization Source: MGI
- PML body organization Source: MGI
- positive regulation of ATPase-coupled calcium transmembrane transporter activity Source: BHF-UCL
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: MGI
- positive regulation of protein-containing complex assembly Source: MGI
- protein localization to nuclear pore Source: MGI
- protein stabilization Source: MGI
- protein sumoylation Source: MGI
- regulation of calcium ion transmembrane transport Source: BHF-UCL
- regulation of cardiac muscle cell contraction Source: BHF-UCL
- regulation of protein stability Source: BHF-UCL
- regulation of transcription, DNA-templated Source: MGI
- roof of mouth development Source: UniProtKB
Keywordsi
Biological process | Stress response, Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-3065676, SUMO is conjugated to E1 (UBA2:SAE1) R-MMU-3065678, SUMO is transferred from E1 to E2 (UBE2I, UBC9) R-MMU-3065679, SUMO is proteolytically processed R-MMU-3108214, SUMOylation of DNA damage response and repair proteins R-MMU-3108232, SUMO E3 ligases SUMOylate target proteins R-MMU-3232118, SUMOylation of transcription factors R-MMU-3232142, SUMOylation of ubiquitinylation proteins R-MMU-3899300, SUMOylation of transcription cofactors R-MMU-4085377, SUMOylation of SUMOylation proteins R-MMU-4090294, SUMOylation of intracellular receptors R-MMU-4551638, SUMOylation of chromatin organization proteins R-MMU-4570464, SUMOylation of RNA binding proteins R-MMU-4615885, SUMOylation of DNA replication proteins R-MMU-4655427, SUMOylation of DNA methylation proteins R-MMU-4755510, SUMOylation of immune response proteins R-MMU-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-MMU-5693571, Nonhomologous End-Joining (NHEJ) R-MMU-5693607, Processing of DNA double-strand break ends R-MMU-5696395, Formation of Incision Complex in GG-NER R-MMU-69473, G2/M DNA damage checkpoint R-MMU-877312, Regulation of IFNG signaling R-MMU-8866904, Negative regulation of activity of TFAP2 (AP-2) family transcription factors R-MMU-9615933, Postmitotic nuclear pore complex (NPC) reformation |
Names & Taxonomyi
Protein namesi | Recommended name: Small ubiquitin-related modifier 1Short name: SUMO-1 Alternative name(s): SMT3 homolog 3 Ubiquitin-homology domain protein PIC1 Ubiquitin-like protein SMT3C Short name: Smt3C |
Gene namesi | Name:Sumo1 Synonyms:Smt3c, Smt3h3, Ubl1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1197010, Sumo1 |
VEuPathDBi | HostDB:ENSMUSG00000026021 |
Subcellular locationi
Nucleus
- Nucleus membrane By similarity
- Nucleus speckle 1 Publication
- PML body By similarity
- Nucleus By similarity
Plasma membrane
- Cell membrane By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: Recruited by BCL11A into the nuclear body (PubMed:18681895). In the presence of ZFHX3, sequesterd to nuclear body (NB)-like dots in the nucleus some of which overlap or closely associate with PML body (By similarity).By similarity1 Publication
Cytosol
- cytosol Source: Reactome
Nucleus
- fibrillar center Source: MGI
- nuclear body Source: UniProtKB
- nuclear membrane Source: MGI
- nuclear speck Source: UniProtKB-SubCell
- nuclear stress granule Source: UniProtKB
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
- PML body Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
- voltage-gated potassium channel complex Source: Ensembl
Other locations
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000035941 | 2 – 97 | Small ubiquitin-related modifier 1Add BLAST | 96 | |
PropeptideiPRO_0000035942 | 98 – 101 | By similarity | 4 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 2 | PhosphoserineCombined sources | 1 | |
Cross-linki | 7 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 7 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 9 | PhosphoserineBy similarity | 1 | |
Cross-linki | 16 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 17 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 23 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 25 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity | ||
Modified residuei | 32 | PhosphoserineBy similarity | 1 | |
Cross-linki | 37 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 39 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 45 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 46 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 97 | Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P63166 |
jPOSTi | P63166 |
MaxQBi | P63166 |
PaxDbi | P63166 |
PeptideAtlasi | P63166 |
PRIDEi | P63166 |
ProteomicsDBi | 257507 |
PTM databases
iPTMneti | P63166 |
PhosphoSitePlusi | P63166 |
SwissPalmi | P63166 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000026021, Expressed in embryonic facial prominence and 91 other tissues |
Genevisiblei | P63166, MM |
Interactioni
Subunit structurei
Covalently attached to KCNB1; UBE2I increases cross-linking with KCNB1 and PIAS1 decreases cross-links with KCNB1 (By similarity).
Interacts with SAE2, RANBP2, PIAS1 and PIAS2 (By similarity).
Interacts with PRKN (By similarity). Covalently attached to a number of proteins such as IKFZ1, PML, RANGAP1, HIPK2, SP100, p53, p73-alpha, MDM2, JUN, DNMT3B and TDG (By similarity).
Also interacts with HIF1A, HIPK2, HIPK3, CHD3, EXOSC9, RAD51 and RAD52 (By similarity).
Interacts with USP25 (via ts SIM domain); the interaction weakly sumoylates USP25 (By similarity).
Interacts with SIMC1, CASP8AP2, RNF111 AND SOBP (via SIM domains) (By similarity).
Interacts with BHLHE40/DEC1 (By similarity).
Interacts with RWDD3 (By similarity).
Interacts with UBE2I/UBC9 and this interaction is enhanced in the presence of RWDD3 (By similarity).
Interacts with MTA1 (By similarity).
Interacts with SENP2 (By similarity).
Interacts with HINT1 (PubMed:31088288).
By similarity1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 36 | Interaction with PIAS2By similarity | 1 |
Binary interactionsi
P63166
GO - Molecular functioni
- enzyme binding Source: MGI
- small protein activating enzyme binding Source: MGI
- transcription factor binding Source: AgBase
- ubiquitin-like protein ligase binding Source: GO_Central
- ubiquitin protein ligase binding Source: UniProtKB
- ubiquitin-specific protease binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204420, 15 interactors |
CORUMi | P63166 |
DIPi | DIP-29278N |
IntActi | P63166, 14 interactors |
MINTi | P63166 |
STRINGi | 10090.ENSMUSP00000088935 |
Miscellaneous databases
RNActi | P63166, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 97 | Ubiquitin-likePROSITE-ProRule annotationAdd BLAST | 78 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1769, Eukaryota |
GeneTreei | ENSGT00940000154319 |
HOGENOMi | CLU_148322_0_0_1 |
InParanoidi | P63166 |
OMAi | TIECHIE |
OrthoDBi | 1583700at2759 |
PhylomeDBi | P63166 |
TreeFami | TF315116 |
Family and domain databases
CDDi | cd16114, Ubl_SUMO1, 1 hit |
InterProi | View protein in InterPro IPR022617, Rad60/SUMO-like_dom IPR046332, SUMO1_Ubl IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF11976, Rad60-SLD, 1 hit |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50053, UBIQUITIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES
60 70 80 90 100
YCQRQGVPMN SLRFLFEGQR IADNHTPKEL GMEEEDVIEV YQEQTGGHST
V
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A087WRQ4 | A0A087WRQ4_MOUSE | Small ubiquitin-related modifier 1 | Sumo1 | 75 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF033353 mRNA Translation: AAC39959.1 AK002536 mRNA Translation: BAB22172.1 AK011074 mRNA Translation: BAB27379.1 AK089081 mRNA Translation: BAC40739.1 AK159366 mRNA Translation: BAE35024.1 BC082566 mRNA Translation: AAH82566.1 BC083158 mRNA Translation: AAH83158.1 |
CCDSi | CCDS35586.1 |
RefSeqi | NP_033486.1, NM_009460.2 |
Genome annotation databases
Ensembli | ENSMUST00000091374; ENSMUSP00000088935; ENSMUSG00000026021 |
GeneIDi | 22218 |
KEGGi | mmu:22218 |
UCSCi | uc007bdx.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF033353 mRNA Translation: AAC39959.1 AK002536 mRNA Translation: BAB22172.1 AK011074 mRNA Translation: BAB27379.1 AK089081 mRNA Translation: BAC40739.1 AK159366 mRNA Translation: BAE35024.1 BC082566 mRNA Translation: AAH82566.1 BC083158 mRNA Translation: AAH83158.1 |
CCDSi | CCDS35586.1 |
RefSeqi | NP_033486.1, NM_009460.2 |
3D structure databases
AlphaFoldDBi | P63166 |
BMRBi | P63166 |
SMRi | P63166 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204420, 15 interactors |
CORUMi | P63166 |
DIPi | DIP-29278N |
IntActi | P63166, 14 interactors |
MINTi | P63166 |
STRINGi | 10090.ENSMUSP00000088935 |
PTM databases
iPTMneti | P63166 |
PhosphoSitePlusi | P63166 |
SwissPalmi | P63166 |
Proteomic databases
EPDi | P63166 |
jPOSTi | P63166 |
MaxQBi | P63166 |
PaxDbi | P63166 |
PeptideAtlasi | P63166 |
PRIDEi | P63166 |
ProteomicsDBi | 257507 |
Protocols and materials databases
Antibodypediai | 3793, 1178 antibodies from 45 providers |
DNASUi | 22218 |
Genome annotation databases
Ensembli | ENSMUST00000091374; ENSMUSP00000088935; ENSMUSG00000026021 |
GeneIDi | 22218 |
KEGGi | mmu:22218 |
UCSCi | uc007bdx.1, mouse |
Organism-specific databases
CTDi | 7341 |
MGIi | MGI:1197010, Sumo1 |
VEuPathDBi | HostDB:ENSMUSG00000026021 |
Phylogenomic databases
eggNOGi | KOG1769, Eukaryota |
GeneTreei | ENSGT00940000154319 |
HOGENOMi | CLU_148322_0_0_1 |
InParanoidi | P63166 |
OMAi | TIECHIE |
OrthoDBi | 1583700at2759 |
PhylomeDBi | P63166 |
TreeFami | TF315116 |
Enzyme and pathway databases
Reactomei | R-MMU-3065676, SUMO is conjugated to E1 (UBA2:SAE1) R-MMU-3065678, SUMO is transferred from E1 to E2 (UBE2I, UBC9) R-MMU-3065679, SUMO is proteolytically processed R-MMU-3108214, SUMOylation of DNA damage response and repair proteins R-MMU-3108232, SUMO E3 ligases SUMOylate target proteins R-MMU-3232118, SUMOylation of transcription factors R-MMU-3232142, SUMOylation of ubiquitinylation proteins R-MMU-3899300, SUMOylation of transcription cofactors R-MMU-4085377, SUMOylation of SUMOylation proteins R-MMU-4090294, SUMOylation of intracellular receptors R-MMU-4551638, SUMOylation of chromatin organization proteins R-MMU-4570464, SUMOylation of RNA binding proteins R-MMU-4615885, SUMOylation of DNA replication proteins R-MMU-4655427, SUMOylation of DNA methylation proteins R-MMU-4755510, SUMOylation of immune response proteins R-MMU-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-MMU-5693571, Nonhomologous End-Joining (NHEJ) R-MMU-5693607, Processing of DNA double-strand break ends R-MMU-5696395, Formation of Incision Complex in GG-NER R-MMU-69473, G2/M DNA damage checkpoint R-MMU-877312, Regulation of IFNG signaling R-MMU-8866904, Negative regulation of activity of TFAP2 (AP-2) family transcription factors R-MMU-9615933, Postmitotic nuclear pore complex (NPC) reformation |
Miscellaneous databases
BioGRID-ORCSi | 22218, 4 hits in 70 CRISPR screens |
ChiTaRSi | Sumo1, mouse |
PROi | PR:P63166 |
RNActi | P63166, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026021, Expressed in embryonic facial prominence and 91 other tissues |
Genevisiblei | P63166, MM |
Family and domain databases
CDDi | cd16114, Ubl_SUMO1, 1 hit |
InterProi | View protein in InterPro IPR022617, Rad60/SUMO-like_dom IPR046332, SUMO1_Ubl IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF11976, Rad60-SLD, 1 hit |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50053, UBIQUITIN_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SUMO1_MOUSE | |
Accessioni | P63166Primary (citable) accession number: P63166 Secondary accession number(s): P55856, Q3TX92, Q93068 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2004 |
Last sequence update: | September 27, 2004 | |
Last modified: | May 25, 2022 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families