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Protein

Ubiquitin-conjugating enzyme E2 B

Gene

UBE2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.5 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme activity Source: GO_Central
  • ubiquitin protein ligase binding Source: UniProtKB
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B6 2681
ReactomeiR-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex
R-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiP63146
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 B (EC:2.3.2.231 Publication)
Alternative name(s):
E2 ubiquitin-conjugating enzyme B
RAD6 homolog B
Short name:
HR6B
Short name:
hHR6B
Ubiquitin carrier protein B
Ubiquitin-conjugating enzyme E2-17 kDa
Ubiquitin-protein ligase B
Gene namesi
Name:UBE2B
Synonyms:RAD6B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000119048.7
HGNCiHGNC:12473 UBE2B
MIMi179095 gene
neXtProtiNX_P63146

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi7320
OpenTargetsiENSG00000119048
PharmGKBiPA37123

Chemistry databases

ChEMBLiCHEMBL3784907

Polymorphism and mutation databases

BioMutaiUBE2B
DMDMi52783814

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000824471 – 152Ubiquitin-conjugating enzyme E2 BAdd BLAST152

Proteomic databases

EPDiP63146
MaxQBiP63146
PaxDbiP63146
PeptideAtlasiP63146
PRIDEiP63146
ProteomicsDBi57496
TopDownProteomicsiP63146

PTM databases

iPTMnetiP63146
PhosphoSitePlusiP63146

Expressioni

Gene expression databases

BgeeiENSG00000119048 Expressed in 230 organ(s), highest expression level in caudate nucleus
CleanExiHS_UBE2B
ExpressionAtlasiP63146 baseline and differential
GenevisibleiP63146 HS

Organism-specific databases

HPAiHPA003875
HPA051765
HPA065898

Interactioni

Subunit structurei

Interacts with RAD18, UBR2 and WAC.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113168, 78 interactors
DIPiDIP-29832N
IntActiP63146, 21 interactors
MINTiP63146
STRINGi9606.ENSP00000265339

Structurei

Secondary structure

1152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP63146
SMRiP63146
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63146

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0419 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00900000140930
HOGENOMiHOG000233454
HOVERGENiHBG063308
InParanoidiP63146
KOiK10574
OMAiCHAIING
OrthoDBiEOG091G0UM8
PhylomeDBiP63146
TreeFamiTF101128

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P63146-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED
60 70 80 90 100
GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY
110 120 130 140 150
DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN

DS
Length:152
Mass (Da):17,312
Last modified:September 27, 2004 - v1
Checksum:iCFDEEEE7E06840BE
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YA80H0YA80_HUMAN
Ubiquitin-conjugating enzyme E2 B
UBE2B
138Annotation score:
H0Y9V2H0Y9V2_HUMAN
Ubiquitin-conjugating enzyme E2 B
UBE2B
141Annotation score:
D6RDW8D6RDW8_HUMAN
Ubiquitin-conjugating enzyme E2 B
UBE2B
44Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22 – 23VG → C (PubMed:1883845).Curated2
Sequence conflicti41F → I (PubMed:1883845).Curated1
Sequence conflicti54K → R (PubMed:1883845).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74525 mRNA Translation: AAA35982.1
X53251 mRNA Translation: CAA37339.1
BT007071 mRNA Translation: AAP35734.1
CR407634 mRNA Translation: CAG28562.1
DQ090910 Genomic DNA Translation: AAY68224.1
AK312012 mRNA Translation: BAG34950.1
CH471062 Genomic DNA Translation: EAW62257.1
CH471062 Genomic DNA Translation: EAW62258.1
BC005979 mRNA Translation: AAH05979.1
BC008404 mRNA Translation: AAH08404.1
BC008470 mRNA Translation: AAH08470.1
CCDSiCCDS4174.1
PIRiB41222
RefSeqiNP_003328.1, NM_003337.3
UniGeneiHs.612096
Hs.730071

Genome annotation databases

EnsembliENST00000265339; ENSP00000265339; ENSG00000119048
GeneIDi7320
KEGGihsa:7320
UCSCiuc003kzh.4 human

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74525 mRNA Translation: AAA35982.1
X53251 mRNA Translation: CAA37339.1
BT007071 mRNA Translation: AAP35734.1
CR407634 mRNA Translation: CAG28562.1
DQ090910 Genomic DNA Translation: AAY68224.1
AK312012 mRNA Translation: BAG34950.1
CH471062 Genomic DNA Translation: EAW62257.1
CH471062 Genomic DNA Translation: EAW62258.1
BC005979 mRNA Translation: AAH05979.1
BC008404 mRNA Translation: AAH08404.1
BC008470 mRNA Translation: AAH08470.1
CCDSiCCDS4174.1
PIRiB41222
RefSeqiNP_003328.1, NM_003337.3
UniGeneiHs.612096
Hs.730071

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JASNMR-A1-152[»]
1NXAmodel-A1-152[»]
2Y4WNMR-A1-152[»]
2YB6X-ray1.50A1-152[»]
2YBFX-ray2.00A1-152[»]
ProteinModelPortaliP63146
SMRiP63146
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113168, 78 interactors
DIPiDIP-29832N
IntActiP63146, 21 interactors
MINTiP63146
STRINGi9606.ENSP00000265339

Chemistry databases

ChEMBLiCHEMBL3784907

PTM databases

iPTMnetiP63146
PhosphoSitePlusiP63146

Polymorphism and mutation databases

BioMutaiUBE2B
DMDMi52783814

Proteomic databases

EPDiP63146
MaxQBiP63146
PaxDbiP63146
PeptideAtlasiP63146
PRIDEiP63146
ProteomicsDBi57496
TopDownProteomicsiP63146

Protocols and materials databases

DNASUi7320
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265339; ENSP00000265339; ENSG00000119048
GeneIDi7320
KEGGihsa:7320
UCSCiuc003kzh.4 human

Organism-specific databases

CTDi7320
DisGeNETi7320
EuPathDBiHostDB:ENSG00000119048.7
GeneCardsiUBE2B
HGNCiHGNC:12473 UBE2B
HPAiHPA003875
HPA051765
HPA065898
MIMi179095 gene
neXtProtiNX_P63146
OpenTargetsiENSG00000119048
PharmGKBiPA37123
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0419 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00900000140930
HOGENOMiHOG000233454
HOVERGENiHBG063308
InParanoidiP63146
KOiK10574
OMAiCHAIING
OrthoDBiEOG091G0UM8
PhylomeDBiP63146
TreeFamiTF101128

Enzyme and pathway databases

UniPathwayi
UPA00143

BRENDAi2.3.2.B6 2681
ReactomeiR-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex
R-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiP63146

Miscellaneous databases

ChiTaRSiUBE2B human
EvolutionaryTraceiP63146
GeneWikiiUBE2B
GenomeRNAii7320
PROiPR:P63146
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000119048 Expressed in 230 organ(s), highest expression level in caudate nucleus
CleanExiHS_UBE2B
ExpressionAtlasiP63146 baseline and differential
GenevisibleiP63146 HS

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBE2B_HUMAN
AccessioniPrimary (citable) accession number: P63146
Secondary accession number(s): B2R503
, D3DQA2, P23567, Q4PJ15, Q9D0J6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: November 7, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
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