Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nuclear transcription factor Y subunit beta

Gene

Nfyb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi59 – 65By similarity7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear transcription factor Y subunit beta
Alternative name(s):
CAAT box DNA-binding protein subunit B
Nuclear transcription factor Y subunit B
Short name:
NF-YB
Gene namesi
Name:Nfyb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:97317 Nfyb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002046101 – 207Nuclear transcription factor Y subunit betaAdd BLAST207

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki140Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitination at Lys-140 plays an important role in transcriptional activation by allowing the deposition of histone H3 methylations as well as histone H2B monoubiquitination at 'Lys-121'.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP63139
MaxQBiP63139
PaxDbiP63139
PRIDEiP63139

PTM databases

PhosphoSitePlusiP63139

Expressioni

Gene expression databases

BgeeiENSMUSG00000020248 Expressed in 292 organ(s), highest expression level in embryo
CleanExiMM_NFYB
ExpressionAtlasiP63139 baseline and differential
GenevisibleiP63139 MM

Interactioni

Subunit structurei

Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding. Interacts with C1QBP (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-89 CCAAT-binding factor complex
STRINGi10090.ENSMUSP00000122403

Structurei

3D structure databases

ProteinModelPortaliP63139
SMRiP63139
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 52A domainAdd BLAST52
Regioni53 – 142B domainAdd BLAST90
Regioni86 – 97Subunit association domain (SAD)By similarityAdd BLAST12
Regioni143 – 207C domainAdd BLAST65

Domaini

Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.

Sequence similaritiesi

Belongs to the NFYB/HAP3 subunit family.Curated

Phylogenomic databases

eggNOGiKOG0869 Eukaryota
COG2036 LUCA
GeneTreeiENSGT00550000074689
HOVERGENiHBG008864
InParanoidiP63139
KOiK08065
OMAiKENFREQ
OrthoDBiEOG091G0Y4D
TreeFamiTF314521

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR003958 CBFA_NFYB_domain
IPR009072 Histone-fold
IPR003956 Transcrpt_fac_NFYB/HAP3_CS
PfamiView protein in Pfam
PF00808 CBFD_NFYB_HMF, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00685 NFYB_HAP3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket
Note: 3 isoforms may be produced.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: P63139-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTMDGDSSTT DASQLGISAD YIGGSHYVIQ PHDDTEDSMN DHEDTNGSKE
60 70 80 90 100
SFREQDIYLP IANVARIMKN AIPQTGKIAK DAKECVQECV SEFISFITSE
110 120 130 140 150
ASERCHQEKR KTINGEDILF AMSTLGFDSY VEPLKLYLQK FREAMKGEKG
160 170 180 190 200
IGGAVSATDG LSEELTEEAF TNQLPAGLIT ADGQQQNVMV YTTSYQQISG

VQQIQFS
Length:207
Mass (Da):22,787
Last modified:September 13, 2004 - v1
Checksum:i1ADFB4B04A3CFC22
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z723D3Z723_MOUSE
Nuclear transcription factor Y subu...
Nfyb
106Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55316 mRNA Translation: CAA39024.1
AK010762 mRNA Translation: BAB27166.1
AK146168 mRNA Translation: BAE26948.1
BC010719 mRNA Translation: AAH10719.1
CCDSiCCDS48654.1
PIRiF38245
RefSeqiNP_035044.1, NM_010914.2 [P63139-1]
XP_011241681.1, XM_011243379.2 [P63139-1]
UniGeneiMm.245998

Genome annotation databases

EnsembliENSMUST00000130911; ENSMUSP00000122403; ENSMUSG00000020248 [P63139-1]
GeneIDi18045
KEGGimmu:18045
UCSCiuc007gjv.1 mouse

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55316 mRNA Translation: CAA39024.1
AK010762 mRNA Translation: BAB27166.1
AK146168 mRNA Translation: BAE26948.1
BC010719 mRNA Translation: AAH10719.1
CCDSiCCDS48654.1
PIRiF38245
RefSeqiNP_035044.1, NM_010914.2 [P63139-1]
XP_011241681.1, XM_011243379.2 [P63139-1]
UniGeneiMm.245998

3D structure databases

ProteinModelPortaliP63139
SMRiP63139
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-89 CCAAT-binding factor complex
STRINGi10090.ENSMUSP00000122403

PTM databases

PhosphoSitePlusiP63139

Proteomic databases

EPDiP63139
MaxQBiP63139
PaxDbiP63139
PRIDEiP63139

Protocols and materials databases

DNASUi18045
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000130911; ENSMUSP00000122403; ENSMUSG00000020248 [P63139-1]
GeneIDi18045
KEGGimmu:18045
UCSCiuc007gjv.1 mouse

Organism-specific databases

CTDi4801
MGIiMGI:97317 Nfyb

Phylogenomic databases

eggNOGiKOG0869 Eukaryota
COG2036 LUCA
GeneTreeiENSGT00550000074689
HOVERGENiHBG008864
InParanoidiP63139
KOiK08065
OMAiKENFREQ
OrthoDBiEOG091G0Y4D
TreeFamiTF314521

Miscellaneous databases

PROiPR:P63139
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020248 Expressed in 292 organ(s), highest expression level in embryo
CleanExiMM_NFYB
ExpressionAtlasiP63139 baseline and differential
GenevisibleiP63139 MM

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR003958 CBFA_NFYB_domain
IPR009072 Histone-fold
IPR003956 Transcrpt_fac_NFYB/HAP3_CS
PfamiView protein in Pfam
PF00808 CBFD_NFYB_HMF, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00685 NFYB_HAP3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNFYB_MOUSE
AccessioniPrimary (citable) accession number: P63139
Secondary accession number(s): P22569, Q3UK54
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again