UniProtKB - P63136 (POK25_HUMAN)
Protein
Endogenous retrovirus group K member 25 Pol protein
Gene
ERVK-25
Organism
Homo sapiens (Human)
Status
Functioni
Early post-infection, the reverse transcriptase converts the viral RNA genome into double-stranded viral DNA. The RNase H domain of the reverse transcriptase performs two functions. It degrades the RNA template and specifically removes the RNA primer from the RNA/DNA hybrid. Following nuclear import, the integrase catalyzes the insertion of the linear, double-stranded viral DNA into the host cell chromosome. Endogenous Pol proteins may have kept, lost or modified their original function during evolution (By similarity).By similarity
Miscellaneous
This protein is synthesized as Gag-Pro and Gag-Pro-Pol polyprotein precursors. These polyproteins are thought, by similarity with type-B retroviruses, to be generated by -1 frameshifts occurring at the Gag-Pro and Pro-Pol genes boundaries.
Exact N-terminus of this protein has not been formally described.
Catalytic activityi
- a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)PROSITE-ProRule annotationEC:2.7.7.49PROSITE-ProRule annotation
- Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation EC:3.1.26.4
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 585 – 626 | Integrase-typePROSITE-ProRule annotationAdd BLAST | 42 | |
DNA bindingi | 809 – 857 | Integrase-typePROSITE-ProRule annotationAdd BLAST | 49 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- RNA-directed DNA polymerase activity Source: UniProtKB-KW
- RNA-DNA hybrid ribonuclease activity Source: UniProtKB-EC
- RNA stem-loop binding Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- DNA integration Source: UniProtKB-KW
- DNA recombination Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Endonuclease, Hydrolase, Multifunctional enzyme, Nuclease, Nucleotidyltransferase, RNA-directed DNA polymerase, Transferase |
Biological process | DNA integration, DNA recombination |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ERVK-25 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:39039, ERVK-25 |
neXtProti | NX_P63136 |
Pathology & Biotechi
Miscellaneous databases
Pharosi | P63136, Tdark |
Genetic variation databases
BioMutai | HGNC:39039 |
DMDMi | 52000774 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186772 | 1 – 954 | Endogenous retrovirus group K member 25 Pol proteinAdd BLAST | 954 |
Proteomic databases
PeptideAtlasi | P63136 |
PRIDEi | P63136 |
ProteomicsDBi | 57494 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 57 – 245 | Reverse transcriptasePROSITE-ProRule annotationAdd BLAST | 189 | |
Domaini | 461 – 588 | RNase HPROSITE-ProRule annotationAdd BLAST | 128 | |
Domaini | 640 – 801 | Integrase catalyticPROSITE-ProRule annotationAdd BLAST | 162 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 161 – 164 | LPQG | 4 | |
Motifi | 195 – 198 | YXDD | 4 |
Domaini
The LPQG and YXDD motifs are catalytically important and conserved among many retroviruses.
Sequence similaritiesi
Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 585 – 626 | Integrase-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
PhylomeDBi | P63136 |
Family and domain databases
Gene3Di | 1.10.10.200, 1 hit 2.30.30.10, 1 hit 3.30.420.10, 2 hits 3.30.70.270, 2 hits |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR017856, Integrase-like_N IPR036862, Integrase_C_dom_sf_retrovir IPR001037, Integrase_C_retrovir IPR001584, Integrase_cat-core IPR003308, Integrase_Zn-bd_dom_N IPR043128, Rev_trsase/Diguanyl_cyclase IPR012337, RNaseH-like_sf IPR002156, RNaseH_domain IPR036397, RNaseH_sf IPR000477, RT_dom IPR010661, RVT_thumb |
Pfami | View protein in Pfam PF00552, IN_DBD_C, 1 hit PF02022, Integrase_Zn, 1 hit PF00075, RNase_H, 1 hit PF00665, rve, 1 hit PF00078, RVT_1, 1 hit PF06817, RVT_thumb, 1 hit |
SUPFAMi | SSF46919, SSF46919, 1 hit SSF50122, SSF50122, 1 hit SSF53098, SSF53098, 2 hits SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50994, INTEGRASE, 1 hit PS51027, INTEGRASE_DBD, 1 hit PS50879, RNASE_H, 1 hit PS50878, RT_POL, 1 hit PS50876, ZF_INTEGRASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P63136-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
NKSKKRRNRV SFLGAATVEP PKPIPLTWKT EKPVWVNQWP LPKQKLEALH
60 70 80 90 100
LLANEQLEKG HIEPSFSPWN SPVFVIQKKS GKWRMLTDLR AVNAVIQPMG
110 120 130 140 150
PLQPGLPSPA MIPKDWPLII IDLKDCFFTI PLAEQDCEKF AFTIPAINNK
160 170 180 190 200
EPATRFQWKV LPQGMLNSPT ICQTFVGRAL QPVREKFSDC YIIHYIDDIL
210 220 230 240 250
CAAETKDKLI DCYTFLQAEV ANAGLAIASD KIQTSTPFHY LGMQIENRKI
260 270 280 290 300
KPQKIEIRKD TLKALNDFQK LLGDINWIRP TLGIPTYAMS NLFSILRGDS
310 320 330 340 350
DLNSKRMLTP EATKEIKLVE EKIQSAQINR IDPLAPLQLL IFATAHSPTG
360 370 380 390 400
IIIQNTDLVE WSFLPHSTVK TFTLYLDQIA TLIGQTRLRI IKLCGNDPDK
410 420 430 440 450
IVVPLTKEQV RQAFINSGAW QIGLANFVGI IDNHYPKTKI FQFLKLTTWI
460 470 480 490 500
LPKITRREPL ENALTVFTDG SSNGKAAYTG PKERVIKTPY QSAQRAELVA
510 520 530 540 550
VITVLQDFDI NIISDSAYVV QATRDVETAL IKYSMDDQLN QLFNLLQQTV
560 570 580 590 600
RKRNFPFYIT HIRAHTNLPG PLTKANKQAD LLVSSALIKA QELHALTHVN
610 620 630 640 650
AAGLKNKFDV TWKLAKDIVQ HCTQCQVLHL PTQEAGVNPR GLCPNALWQM
660 670 680 690 700
DVTHVPSFGR LSYVHVTVDT YSHFIWATCH TGESTSHVKK HLLSCFAVMG
710 720 730 740 750
VPEKIKTDNG PGYCSKAFQK FLSQWKISHT TGIPYNSQGQ AIVERTNRTL
760 770 780 790 800
KTQLVKQKEG GDSKECTTPQ MQLNLALYTL NFLNIYRNQT TTSAEQHLTG
810 820 830 840 850
KKNSPHEGKL IWWKDNKNKT WEIGKVITWG RGFACVSPGE NQLPVWIPTR
860 870 880 890 900
HLKFYNEPIR DAKKSTSAET ETPQSSTVDS QDEQNGDVRR TDEVAIHQEG
910 920 930 940 950
RAANLGTTKE ADAVSYKISR EHKGDTNPRE YAACSLDDCI NGGKSPYACR
SSCS
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketP61570 | ENK25_HUMAN | Endogenous retrovirus group K membe... | ERVK-25 | 661 | Annotation score: | ||
P63125 | VPK25_HUMAN | Endogenous retrovirus group K membe... | ERVK-25 | 156 | Annotation score: | ||
P61579 | ERK25_HUMAN | Endogenous retrovirus group K membe... | ERVK-25 | 105 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP000776 Genomic DNA No translation available. |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP000776 Genomic DNA No translation available. |
3D structure databases
SMRi | P63136 |
ModBasei | Search... |
Genetic variation databases
BioMutai | HGNC:39039 |
DMDMi | 52000774 |
Proteomic databases
PeptideAtlasi | P63136 |
PRIDEi | P63136 |
ProteomicsDBi | 57494 |
Organism-specific databases
GeneCardsi | ERVK-25 |
HGNCi | HGNC:39039, ERVK-25 |
neXtProti | NX_P63136 |
GenAtlasi | Search... |
Phylogenomic databases
PhylomeDBi | P63136 |
Miscellaneous databases
Pharosi | P63136, Tdark |
RNActi | P63136, protein |
Family and domain databases
Gene3Di | 1.10.10.200, 1 hit 2.30.30.10, 1 hit 3.30.420.10, 2 hits 3.30.70.270, 2 hits |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR017856, Integrase-like_N IPR036862, Integrase_C_dom_sf_retrovir IPR001037, Integrase_C_retrovir IPR001584, Integrase_cat-core IPR003308, Integrase_Zn-bd_dom_N IPR043128, Rev_trsase/Diguanyl_cyclase IPR012337, RNaseH-like_sf IPR002156, RNaseH_domain IPR036397, RNaseH_sf IPR000477, RT_dom IPR010661, RVT_thumb |
Pfami | View protein in Pfam PF00552, IN_DBD_C, 1 hit PF02022, Integrase_Zn, 1 hit PF00075, RNase_H, 1 hit PF00665, rve, 1 hit PF00078, RVT_1, 1 hit PF06817, RVT_thumb, 1 hit |
SUPFAMi | SSF46919, SSF46919, 1 hit SSF50122, SSF50122, 1 hit SSF53098, SSF53098, 2 hits SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50994, INTEGRASE, 1 hit PS51027, INTEGRASE_DBD, 1 hit PS50879, RNASE_H, 1 hit PS50878, RT_POL, 1 hit PS50876, ZF_INTEGRASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | POK25_HUMAN | |
Accessioni | P63136Primary (citable) accession number: P63136 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2004 |
Last sequence update: | September 13, 2004 | |
Last modified: | August 12, 2020 | |
This is version 91 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
ERV, Reference proteome, Transposable elementDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - SIMILARITY comments
Index of protein domains and families