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Protein

14-3-3 protein zeta/delta

Gene

Ywhaz

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

GO - Molecular functioni

GO - Biological processi

  • establishment of Golgi localization Source: MGI
  • Golgi reassembly Source: MGI
  • histamine secretion by mast cell Source: MGI
  • protein targeting Source: MGI
  • protein targeting to mitochondrion Source: MGI
  • regulation of cell death Source: MGI
  • response to drug Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-111447 Activation of BAD and translocation to mitochondria
R-MMU-1445148 Translocation of GLUT4 to the plasma membrane
R-MMU-3769402 Deactivation of the beta-catenin transactivating complex
R-MMU-392517 Rap1 signalling
R-MMU-430116 GP1b-IX-V activation signalling
R-MMU-450604 KSRP (KHSRP) binds and destabilizes mRNA
R-MMU-512988 Interleukin-3, 5 and GM-CSF signaling
R-MMU-5625740 RHO GTPases activate PKNs
R-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein zeta/delta
Alternative name(s):
Protein kinase C inhibitor protein 1
Short name:
KCIP-1
SEZ-2
Gene namesi
Name:Ywhaz
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:109484 Ywhaz

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586281 – 24514-3-3 protein zeta/deltaAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei3N6-acetyllysineBy similarity1
Modified residuei58Phosphoserine; by PKA1 Publication1
Modified residuei68N6-acetyllysineBy similarity1
Modified residuei184PhosphoserineBy similarity1
Modified residuei207PhosphoserineCombined sources1
Modified residuei210PhosphoserineBy similarity1
Modified residuei232Phosphothreonine; by CK1By similarity1

Post-translational modificationi

The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1 (By similarity). Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53.By similarity1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP63101
MaxQBiP63101
PaxDbiP63101
PeptideAtlasiP63101
PRIDEiP63101
TopDownProteomicsiP63101

2D gel databases

REPRODUCTION-2DPAGEiP63101
UCD-2DPAGEiP63101

PTM databases

iPTMnetiP63101
PhosphoSitePlusiP63101
SwissPalmiP63101

Expressioni

Gene expression databases

BgeeiENSMUSG00000022285
ExpressionAtlasiP63101 baseline and differential
GenevisibleiP63101 MM

Interactioni

Subunit structurei

Homodimer. Heterodimerizes with YWHAE (By similarity). Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58 (By similarity). Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with CDK16 and with WEE1 (C-terminal). Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with BSPRY. Interacts with Thr-phosphorylated ITGB2 (By similarity). Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation (By similarity). It may also prevent thiol-dependent inactivation (By similarity). Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization (By similarity). Interacts with GAB2 (By similarity). Interacts with SAMSN1. Interacts with BCL2L11 and TLK2. Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (By similarity). Interacts with ZFP36L1 (via phosphorylated form); this interaction occurs in a p38 MAPK- and AKT-signaling pathways (PubMed:22701344). Interacts with SLITRK1 (By similarity).By similarity8 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei56Interaction with phosphoserine on interacting proteinBy similarity1
Sitei127Interaction with phosphoserine on interacting proteinBy similarity1

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204623, 175 interactors
ComplexPortaliCPX-1148 Foxo3-Ywhaz complex
CORUMiP63101
DIPiDIP-31894N
IntActiP63101, 705 interactors
MINTiP63101
STRINGi10090.ENSMUSP00000022894

Structurei

3D structure databases

ProteinModelPortaliP63101
SMRiP63101
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841 Eukaryota
COG5040 LUCA
GeneTreeiENSGT00760000119116
HOGENOMiHOG000240379
HOVERGENiHBG050423
InParanoidiP63101
KOiK16197
OMAiLWTSSDS
OrthoDBiEOG091G0VKY
PhylomeDBiP63101
TreeFamiTF102003

Family and domain databases

Gene3Di1.20.190.20, 1 hit
InterProiView protein in InterPro
IPR000308 14-3-3
IPR023409 14-3-3_CS
IPR036815 14-3-3_dom_sf
IPR023410 14-3-3_domain
PANTHERiPTHR18860 PTHR18860, 1 hit
PfamiView protein in Pfam
PF00244 14-3-3, 1 hit
PIRSFiPIRSF000868 14-3-3, 1 hit
PRINTSiPR00305 1433ZETA
SMARTiView protein in SMART
SM00101 14_3_3, 1 hit
SUPFAMiSSF48445 SSF48445, 1 hit
PROSITEiView protein in PROSITE
PS00796 1433_1, 1 hit
PS00797 1433_2, 1 hit

Sequencei

Sequence statusi: Complete.

P63101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKNELVQKA KLAEQAERYD DMAACMKSVT EQGAELSNEE RNLLSVAYKN
60 70 80 90 100
VVGARRSSWR VVSSIEQKTE GAEKKQQMAR EYREKIETEL RDICNDVLSL
110 120 130 140 150
LEKFLIPNAS QPESKVFYLK MKGDYYRYLA EVAAGDDKKG IVDQSQQAYQ
160 170 180 190 200
EAFEISKKEM QPTHPIRLGL ALNFSVFYYE ILNSPEKACS LAKTAFDEAI
210 220 230 240
AELDTLSEES YKDSTLIMQL LRDNLTLWTS DTQGDEAEAG EGGEN
Length:245
Mass (Da):27,771
Last modified:September 13, 2004 - v1
Checksum:i2164DF3793B45B7A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78M → V in BAA11751 (PubMed:9016762).Curated1
Sequence conflicti139K → R in BAE36724 (PubMed:16141072).Curated1
Sequence conflicti218 – 219MQ → IE in BAA13421 (Ref. 2) Curated2
Sequence conflicti236E → D in BAA11751 (PubMed:9016762).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78647 mRNA Translation: BAA11464.1
D87660 mRNA Translation: BAA13421.1
U79231 mRNA Translation: AAC53254.1
D83037 mRNA Translation: BAA11751.1
AK083368 mRNA Translation: BAC38887.1
AK145657 mRNA Translation: BAE26570.1
AK146800 mRNA Translation: BAE27442.1
AK150381 mRNA Translation: BAE29512.1
AK151900 mRNA Translation: BAE30783.1
AK162099 mRNA Translation: BAE36724.1
AK167128 mRNA Translation: BAE39275.1
BC050891 mRNA Translation: AAH50891.1
BC089334 mRNA Translation: AAH89334.1
CCDSiCCDS27432.1
PIRiJC5384
RefSeqiNP_001240734.1, NM_001253805.1
NP_001240735.1, NM_001253806.1
NP_035870.1, NM_011740.3
XP_011243654.1, XM_011245352.2
UniGeneiMm.3360

Genome annotation databases

EnsembliENSMUST00000022894; ENSMUSP00000022894; ENSMUSG00000022285
ENSMUST00000110361; ENSMUSP00000105990; ENSMUSG00000022285
ENSMUST00000110362; ENSMUSP00000105991; ENSMUSG00000022285
GeneIDi22631
KEGGimmu:22631
UCSCiuc007vmz.2 mouse

Similar proteinsi

Entry informationi

Entry namei1433Z_MOUSE
AccessioniPrimary (citable) accession number: P63101
Secondary accession number(s): P35215
, P70197, P97286, Q3TSF1, Q5EBQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: July 18, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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