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Protein

Calcineurin subunit B type 1

Gene

PPP3R1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.1 Publication

Miscellaneous

This protein has four functional calcium-binding sites (PubMed:8524402, PubMed:12218175, PubMed:12357034, PubMed:17498738, PubMed:22343722, PubMed:23468591, PubMed:26794871, PubMed:27974827). Although African swine fever virus infects pigs and not humans, human PPP3R1 and PPP3CA have been used for the crystallization. PPP3CA and PPP3R1 interact with African swine fever virus Mal-047/A238L (via PKIIIT and FLCVK motifs); the interaction does not block catalytic activity per se but inhibits PPP3CA function by blocking the access to the two substrate recognition (PubMed:23468591).8 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi31 – 421Combined sources8 PublicationsAdd BLAST12
Calcium bindingi63 – 742Combined sources8 PublicationsAdd BLAST12
Calcium bindingi100 – 1113Combined sources8 PublicationsAdd BLAST12
Calcium bindingi141 – 1524Combined sources8 PublicationsAdd BLAST12

GO - Molecular functioni

  • calcium-dependent protein serine/threonine phosphatase activity Source: UniProtKB
  • calcium ion binding Source: UniProtKB
  • calmodulin binding Source: UniProtKB
  • cyclosporin A binding Source: UniProtKB
  • phosphatase binding Source: UniProtKB
  • protein domain specific binding Source: BHF-UCL

GO - Biological processi

Keywordsi

LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-111447 Activation of BAD and translocation to mitochondria
R-HSA-180024 DARPP-32 events
R-HSA-2025928 Calcineurin activates NFAT
R-HSA-2871809 FCERI mediated Ca+2 mobilization
R-HSA-4086398 Ca2+ pathway
R-HSA-5607763 CLEC7A (Dectin-1) induces NFAT activation
SIGNORiP63098

Names & Taxonomyi

Protein namesi
Recommended name:
Calcineurin subunit B type 1
Alternative name(s):
Protein phosphatase 2B regulatory subunit 1
Protein phosphatase 3 regulatory subunit B alpha isoform 1
Gene namesi
Name:PPP3R1
Synonyms:CNA2, CNB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000221823.10
HGNCiHGNC:9317 PPP3R1
MIMi601302 gene
neXtProtiNX_P63098

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5534
OpenTargetsiENSG00000221823
PharmGKBiPA33681

Chemistry databases

DrugBankiDB04906 ISA247
DB08231 MYRISTIC ACID

Polymorphism and mutation databases

BioMutaiPPP3R1
DMDMi52000904

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000734842 – 170Calcineurin subunit B type 1Add BLAST169

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Modified residuei106PhosphotyrosineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiP63098
MaxQBiP63098
PaxDbiP63098
PeptideAtlasiP63098
PRIDEiP63098
ProteomicsDBi57476
TopDownProteomicsiP63098

2D gel databases

OGPiP63098

PTM databases

iPTMnetiP63098
PhosphoSitePlusiP63098
SwissPalmiP63098

Expressioni

Gene expression databases

BgeeiENSG00000221823 Expressed in 207 organ(s), highest expression level in caudate nucleus
CleanExiHS_PPP3R1
ExpressionAtlasiP63098 baseline and differential
GenevisibleiP63098 HS

Organism-specific databases

HPAiCAB005610
HPA047157

Interactioni

Subunit structurei

Forms a complex composed of a calmodulin-dependent catalytic subunit (also known as calcineurin A) and a regulatory Ca2+-binding subunit (also known as calcineurin B) (PubMed:8524402, PubMed:12218175, PubMed:12357034, PubMed:17498738, PubMed:22343722, PubMed:23468591, PubMed:26794871, PubMed:27974827). There are three catalytic subunits, each encoded by a separate gene (PPP3CA, PPP3CB, and PPP3CC) and two regulatory subunits which are also encoded by separate genes (PPP3R1 and PPP3R2). Interacts with catalytic subunit PPP3CA/calcineurin A (PubMed:8524402, PubMed:12218175, PubMed:12357034, PubMed:17498738, PubMed:22343722, PubMed:23468591, PubMed:27974827). Interacts with catalytic subunit PPP3CB/calcineurin A (PubMed:26794871). Interacts with CIB1 (via C-terminal region); the interaction increases upon cardiomyocyte hypertrophy (By similarity). Interacts with RCAN1 (PubMed:12809556).By similarity9 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei118Interaction with PxVP motif in substrates of the catalytic subunit1 Publication1
Sitei122Interaction with PxVP motif in substrates of the catalytic subunit1 Publication1

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111526, 41 interactors
ComplexPortaliCPX-1001 Calcineurin-Calmodulin complex, gamma-R1 variant
CPX-1003 Calcineurin-Calmodulin complex, alpha-R1 variant
CPX-1009 Calcineurin-Calmodulin complex, beta-R1 variant
CPX-1112 Calcineurin-Calmodulin-AKAP5 complex, gamma-R1 variant
CPX-674 Calcineurin-Calmodulin-AKAP5 complex, alpha-R1 variant
CPX-998 Calcineurin-Calmodulin-AKAP5 complex, beta-R1 variant
CORUMiP63098
DIPiDIP-6096N
ELMiP63098
IntActiP63098, 9 interactors
MINTiP63098
STRINGi9606.ENSP00000234310

Structurei

Secondary structure

1170
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00565
ProteinModelPortaliP63098
SMRiP63098
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63098

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 51EF-hand 1PROSITE-ProRule annotationAdd BLAST34
Domaini50 – 85EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini87 – 122EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini128 – 163EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 136Calcineurin A binding8 Publications6

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0034 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00860000133729
HOVERGENiHBG105307
InParanoidiP63098
KOiK06268
PhylomeDBiP63098
TreeFamiTF105558

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13499 EF-hand_7, 2 hits
SMARTiView protein in SMART
SM00054 EFh, 4 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 4 hits
PS50222 EF_HAND_2, 4 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P63098-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ
60 70 80 90 100
QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD
110 120 130 140 150
MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF
160 170
EEFCAVVGGL DIHKKMVVDV
Length:170
Mass (Da):19,300
Last modified:January 23, 2007 - v2
Checksum:iC904715DC0386056
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YTA9D3YTA9_HUMAN
Calcineurin subunit B type 1
PPP3R1
189Annotation score:
F6U1T9F6U1T9_HUMAN
Calcineurin subunit B type 1
PPP3R1
160Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30773 mRNA Translation: AAB08721.1
CR456938 mRNA Translation: CAG33219.1
AK314893 mRNA Translation: BAG37407.1
AC017083 Genomic DNA Translation: AAY14715.1
CH471053 Genomic DNA Translation: EAW99878.1
BC027913 mRNA Translation: AAH27913.1
CCDSiCCDS46310.1
PIRiA33391
RefSeqiNP_000936.1, NM_000945.3
UniGeneiHs.280604

Genome annotation databases

EnsembliENST00000234310; ENSP00000234310; ENSG00000221823
GeneIDi5534
KEGGihsa:5534
UCSCiuc002sei.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30773 mRNA Translation: AAB08721.1
CR456938 mRNA Translation: CAG33219.1
AK314893 mRNA Translation: BAG37407.1
AC017083 Genomic DNA Translation: AAY14715.1
CH471053 Genomic DNA Translation: EAW99878.1
BC027913 mRNA Translation: AAH27913.1
CCDSiCCDS46310.1
PIRiA33391
RefSeqiNP_000936.1, NM_000945.3
UniGeneiHs.280604

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AUIX-ray2.10B2-170[»]
1M63X-ray2.80B/F2-170[»]
1MF8X-ray3.10B1-170[»]
2P6BX-ray2.30B/D16-170[»]
3LL8X-ray2.00B/D16-170[»]
4F0ZX-ray1.70B1-170[»]
4OR9X-ray2.23B1-170[»]
4ORAX-ray2.75B1-170[»]
4ORCX-ray2.70B1-170[»]
5SVEX-ray2.60B1-170[»]
DisProtiDP00565
ProteinModelPortaliP63098
SMRiP63098
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111526, 41 interactors
ComplexPortaliCPX-1001 Calcineurin-Calmodulin complex, gamma-R1 variant
CPX-1003 Calcineurin-Calmodulin complex, alpha-R1 variant
CPX-1009 Calcineurin-Calmodulin complex, beta-R1 variant
CPX-1112 Calcineurin-Calmodulin-AKAP5 complex, gamma-R1 variant
CPX-674 Calcineurin-Calmodulin-AKAP5 complex, alpha-R1 variant
CPX-998 Calcineurin-Calmodulin-AKAP5 complex, beta-R1 variant
CORUMiP63098
DIPiDIP-6096N
ELMiP63098
IntActiP63098, 9 interactors
MINTiP63098
STRINGi9606.ENSP00000234310

Chemistry databases

DrugBankiDB04906 ISA247
DB08231 MYRISTIC ACID

PTM databases

iPTMnetiP63098
PhosphoSitePlusiP63098
SwissPalmiP63098

Polymorphism and mutation databases

BioMutaiPPP3R1
DMDMi52000904

2D gel databases

OGPiP63098

Proteomic databases

EPDiP63098
MaxQBiP63098
PaxDbiP63098
PeptideAtlasiP63098
PRIDEiP63098
ProteomicsDBi57476
TopDownProteomicsiP63098

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000234310; ENSP00000234310; ENSG00000221823
GeneIDi5534
KEGGihsa:5534
UCSCiuc002sei.2 human

Organism-specific databases

CTDi5534
DisGeNETi5534
EuPathDBiHostDB:ENSG00000221823.10
GeneCardsiPPP3R1
HGNCiHGNC:9317 PPP3R1
HPAiCAB005610
HPA047157
MIMi601302 gene
neXtProtiNX_P63098
OpenTargetsiENSG00000221823
PharmGKBiPA33681
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0034 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00860000133729
HOVERGENiHBG105307
InParanoidiP63098
KOiK06268
PhylomeDBiP63098
TreeFamiTF105558

Enzyme and pathway databases

ReactomeiR-HSA-111447 Activation of BAD and translocation to mitochondria
R-HSA-180024 DARPP-32 events
R-HSA-2025928 Calcineurin activates NFAT
R-HSA-2871809 FCERI mediated Ca+2 mobilization
R-HSA-4086398 Ca2+ pathway
R-HSA-5607763 CLEC7A (Dectin-1) induces NFAT activation
SIGNORiP63098

Miscellaneous databases

ChiTaRSiPPP3R1 human
EvolutionaryTraceiP63098
GeneWikiiPPP3R1
GenomeRNAii5534
PROiPR:P63098
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000221823 Expressed in 207 organ(s), highest expression level in caudate nucleus
CleanExiHS_PPP3R1
ExpressionAtlasiP63098 baseline and differential
GenevisibleiP63098 HS

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13499 EF-hand_7, 2 hits
SMARTiView protein in SMART
SM00054 EFh, 4 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 4 hits
PS50222 EF_HAND_2, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCANB1_HUMAN
AccessioniPrimary (citable) accession number: P63098
Secondary accession number(s): B2RC10
, B5MDU4, P06705, P15117, Q08044, Q53SL0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: October 10, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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