UniProtKB - P63087 (PP1G_MOUSE)
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
Ppp1cc
Functioni
Catalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 64 | Manganese 1By similarity | 1 | |
Metal bindingi | 66 | Manganese 1By similarity | 1 | |
Metal bindingi | 92 | Manganese 1By similarity | 1 | |
Metal bindingi | 92 | Manganese 2By similarity | 1 | |
Metal bindingi | 124 | Manganese 2By similarity | 1 | |
Active sitei | 125 | Proton donorBy similarity | 1 | |
Metal bindingi | 173 | Manganese 2By similarity | 1 | |
Metal bindingi | 248 | Manganese 2By similarity | 1 |
GO - Molecular functioni
- lamin binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- phosphatase activity Source: UniProtKB
- protein-containing complex binding Source: MGI
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- protein kinase binding Source: Ensembl
- protein N-terminus binding Source: MGI
- protein phosphatase 1 binding Source: MGI
- protein phosphatase binding Source: MGI
- protein serine/threonine phosphatase activity Source: MGI
- protein serine phosphatase activity Source: UniProtKB-EC
- protein threonine phosphatase activity Source: UniProtKB-EC
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- circadian regulation of gene expression Source: UniProtKB
- entrainment of circadian clock by photoperiod Source: UniProtKB
- glycogen metabolic process Source: UniProtKB-KW
- neuron differentiation Source: MGI
- positive regulation of glial cell proliferation Source: MGI
- protein dephosphorylation Source: UniProtKB
- regulation of circadian rhythm Source: UniProtKB
- regulation of nucleocytoplasmic transport Source: MGI
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Biological process | Biological rhythms, Carbohydrate metabolism, Cell cycle, Cell division, Glycogen metabolism |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-5663220, RHO GTPases Activate Formins R-MMU-5673000, RAF activation R-MMU-68877, Mitotic Prometaphase R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein phosphatase PP1-gamma catalytic subunit (EC:3.1.3.16)Short name: PP-1G Alternative name(s): Protein phosphatase 1C catalytic subunit |
Gene namesi | Name:Ppp1cc |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104872, Ppp1cc |
Subcellular locationi
Cytoskeleton
- microtubule organizing center By similarity
Mitochondrion
- Mitochondrion By similarity
Nucleus
- Nucleus By similarity
- nucleolus By similarity
- nucleoplasm By similarity
- Nucleus speckle By similarity
Other locations
- Cytoplasm By similarity
- Cleavage furrow By similarity
- kinetochore By similarity
- Midbody By similarity
Note: Colocalizes with SPZ1 in the nucleus (PubMed:15226296). Colocalizes with URI1 at mitochondrion. Rapidly exchanges between the nucleolar, nucleoplasmic and cytoplasmic compartments (By similarity). Highly mobile in cells and can be relocalized through interaction with targeting subunits (By similarity). In the presence of PPP1R8 relocalizes from the nucleolus to nuclear speckles (By similarity). Shows a dynamic targeting to specific sites throughout the cell cycle (By similarity). Highly concentrated in nucleoli of interphase cells and localizes at kinetochores early in mitosis (By similarity). Relocalization to chromosome-containing regions occurs at the transition from early to late anaphase (By similarity). Also accumulates at the cleavage furrow and midbody by telophase (By similarity).By similarity1 Publication
Mitochondrion
- mitochondrial outer membrane Source: MGI
- mitochondrion Source: MGI
Nucleus
- nuclear chromosome, telomeric region Source: Ensembl
- nuclear speck Source: UniProtKB-SubCell
- nucleolus Source: UniProtKB-SubCell
- nucleus Source: GO_Central
Plasma Membrane
- cleavage furrow Source: UniProtKB-SubCell
Other locations
- condensed chromosome kinetochore Source: UniProtKB-SubCell
- cytoplasm Source: MGI
- dendritic spine Source: MGI
- glutamatergic synapse Source: Ensembl
- midbody Source: UniProtKB-SubCell
- presynapse Source: MGI
- protein phosphatase type 1 complex Source: InterPro
- PTW/PP1 phosphatase complex Source: UniProtKB
Keywords - Cellular componenti
Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Mitochondrion, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000058788 | 2 – 323 | Serine/threonine-protein phosphatase PP1-gamma catalytic subunitAdd BLAST | 322 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 307 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 311 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P63087 |
jPOSTi | P63087 |
PaxDbi | P63087 |
PeptideAtlasi | P63087 |
PRIDEi | P63087 |
PTM databases
iPTMneti | P63087 |
PhosphoSitePlusi | P63087 |
SwissPalmi | P63087 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000004455, Expressed in retinal neural layer and 85 other tissues |
Genevisiblei | P63087, MM |
Interactioni
Subunit structurei
PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A, PPP1R12B and PPP1R12C mediate binding to myosin. PPP1R3A (in skeletal muscle), PPP1R3B (in liver), PPP1R3C, PPP1R3D and PPP1R3F (in brain) mediate binding to glycogen.
Interacts with PPP1R3B, PPP1R7 and CDCA2 (By similarity).
Isoform 2 interacts with SPZ1. This interaction can prevent SPZ1 binding to the E-box and inhibits PPP1CC activity. PPP1R15A and PPP1R15B mediate binding to EIF2S1.
Part of a complex containing PPP1R15B, PP1 and NCK1/2.
Interacts with IKFZ1; the interaction targets PPP1CC to pericentromeric heterochromatin, dephosphorylates IKAROS, stabilizes it and prevents it from degradation.
Interacts with NOM1 and PPP1R8.
Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA or PPP1CB or PPP1CC.
Interacts with PPP1R8.
Interacts with NEK2.
Interacts with NEK2.
Interacts with URI1; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner (By similarity).
Isoform 2 interacts with PPP1R42; the interaction is direct.
Interacts with FOXP3 (By similarity).
Isoform 2 interacts with TMEM225 (via RVxF motif) (PubMed:25605614). Isoform 2, but not isoform 1, interacts with Sh3glb1 testis-specific isoform 3; this interaction leads to the inhibition of phosphatase activity (PubMed:17381077).
Interacts with MKI67 (By similarity).
Interacts with RRP1B; this targets PPP1CC to the nucleolus (By similarity).
Found in a complex with PPP1CA, PPP1CC, SHC1 and PEAK1 (By similarity) (PubMed:14638860, PubMed:15226296, PubMed:16835242, PubMed:17381077, PubMed:19282287, PubMed:19886865, PubMed:25605614).
Interacts with DYNLT4 (By similarity).
By similarity7 PublicationsGO - Molecular functioni
- lamin binding Source: MGI
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- protein kinase binding Source: Ensembl
- protein N-terminus binding Source: MGI
- protein phosphatase 1 binding Source: MGI
- protein phosphatase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202337, 47 interactors 241685, 3 interactors |
IntActi | P63087, 27 interactors |
MINTi | P63087 |
STRINGi | 10090.ENSMUSP00000099587 |
Miscellaneous databases
RNActi | P63087, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P63087 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0374, Eukaryota |
GeneTreei | ENSGT00940000153472 |
InParanoidi | P63087 |
OMAi | EEHEIRY |
OrthoDBi | 766640at2759 |
PhylomeDBi | P63087 |
TreeFami | TF354243 |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR037981, PPP1CC IPR006186, Ser/Thr-sp_prot-phosphatase IPR031675, STPPase_N |
PANTHERi | PTHR11668:SF204, PTHR11668:SF204, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit PF16891, STPPase_N, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADIDKLNID SIIQRLLEVR GSKPGKNVQL QENEIRGLCL KSREIFLSQP
60 70 80 90 100
ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS
110 120 130 140 150
LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK
160 170 180 190 200
TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL
210 220 230 240 250
LCDLLWSDPD KDVLGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV
260 270 280 290 300
VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAE
310 320
KKKPNATRPV TPPRGMITKQ AKK
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JFF1 | A0A0G2JFF1_MOUSE | Serine/threonine-protein phosphatas... | Ppp1cc | 292 | Annotation score: | ||
A0A0G2JGC1 | A0A0G2JGC1_MOUSE | Serine/threonine-protein phosphatas... | Ppp1cc | 273 | Annotation score: | ||
A0A0G2JF34 | A0A0G2JF34_MOUSE | Serine/threonine-protein phosphatas... | Ppp1cc | 87 | Annotation score: | ||
A0A0G2JE42 | A0A0G2JE42_MOUSE | Serine/threonine-protein phosphatas... | Ppp1cc | 27 | Annotation score: | ||
A0A0G2JEB0 | A0A0G2JEB0_MOUSE | Serine/threonine-protein phosphatas... | Ppp1cc | 26 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 316 – 317 | MI → IV in AAC53383 (PubMed:9339378).Curated | 2 | |
Sequence conflicti | 316 – 317 | MI → IV in AAC53384 (PubMed:9339378).Curated | 2 | |
Sequence conflicti | 316 – 317 | MI → IV in BAA19729 (Ref. 3) Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005095 | 315 – 323 | GMITKQAKK → VGSGLNPSIQKASNYRNNTV LYE in isoform 2. 2 Publications | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27071 mRNA Translation: AAA37526.1 U53456 mRNA Translation: AAC53383.1 U53276 , U53271, U53272, U53273, U53275 Genomic DNA Translation: AAC53384.1 U53276 , U53271, U53272, U53273, U53275 Genomic DNA Translation: AAC53385.1 D85137 mRNA Translation: BAA19729.1 BC010613 mRNA Translation: AAH10613.1 BC021646 mRNA Translation: AAH21646.1 BC085496 mRNA Translation: AAH85496.1 |
CCDSi | CCDS19643.1 [P63087-1] |
PIRi | C32550 |
RefSeqi | NP_038664.2, NM_013636.3 [P63087-1] XP_006508478.1, XM_006508415.3 XP_006530263.1, XM_006530200.2 [P63087-2] |
Genome annotation databases
Ensembli | ENSMUST00000086294; ENSMUSP00000083474; ENSMUSG00000004455 [P63087-2] ENSMUST00000102528; ENSMUSP00000099587; ENSMUSG00000004455 [P63087-1] |
GeneIDi | 19047 |
KEGGi | mmu:19047 |
UCSCi | uc008zks.1, mouse [P63087-1] uc008zkt.1, mouse [P63087-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight The things we forget - Issue 32 of March 2003 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27071 mRNA Translation: AAA37526.1 U53456 mRNA Translation: AAC53383.1 U53276 , U53271, U53272, U53273, U53275 Genomic DNA Translation: AAC53384.1 U53276 , U53271, U53272, U53273, U53275 Genomic DNA Translation: AAC53385.1 D85137 mRNA Translation: BAA19729.1 BC010613 mRNA Translation: AAH10613.1 BC021646 mRNA Translation: AAH21646.1 BC085496 mRNA Translation: AAH85496.1 |
CCDSi | CCDS19643.1 [P63087-1] |
PIRi | C32550 |
RefSeqi | NP_038664.2, NM_013636.3 [P63087-1] XP_006508478.1, XM_006508415.3 XP_006530263.1, XM_006530200.2 [P63087-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4V0U | X-ray | 7.88 | D/F/H/J/N | 1-323 | [»] | |
4V0V | X-ray | 1.61 | A/C | 7-300 | [»] | |
4V0W | X-ray | 1.55 | A/C | 7-300 | [»] | |
4V0X | X-ray | 1.85 | A | 7-300 | [»] | |
SMRi | P63087 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202337, 47 interactors 241685, 3 interactors |
IntActi | P63087, 27 interactors |
MINTi | P63087 |
STRINGi | 10090.ENSMUSP00000099587 |
PTM databases
iPTMneti | P63087 |
PhosphoSitePlusi | P63087 |
SwissPalmi | P63087 |
Proteomic databases
EPDi | P63087 |
jPOSTi | P63087 |
PaxDbi | P63087 |
PeptideAtlasi | P63087 |
PRIDEi | P63087 |
Protocols and materials databases
Antibodypediai | 2879, 413 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000086294; ENSMUSP00000083474; ENSMUSG00000004455 [P63087-2] ENSMUST00000102528; ENSMUSP00000099587; ENSMUSG00000004455 [P63087-1] |
GeneIDi | 19047 |
KEGGi | mmu:19047 |
UCSCi | uc008zks.1, mouse [P63087-1] uc008zkt.1, mouse [P63087-2] |
Organism-specific databases
CTDi | 5501 |
MGIi | MGI:104872, Ppp1cc |
Phylogenomic databases
eggNOGi | KOG0374, Eukaryota |
GeneTreei | ENSGT00940000153472 |
InParanoidi | P63087 |
OMAi | EEHEIRY |
OrthoDBi | 766640at2759 |
PhylomeDBi | P63087 |
TreeFami | TF354243 |
Enzyme and pathway databases
Reactomei | R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-5663220, RHO GTPases Activate Formins R-MMU-5673000, RAF activation R-MMU-68877, Mitotic Prometaphase R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Miscellaneous databases
BioGRID-ORCSi | 19047, 4 hits in 19 CRISPR screens |
ChiTaRSi | Ppp1cc, mouse |
PROi | PR:P63087 |
RNActi | P63087, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000004455, Expressed in retinal neural layer and 85 other tissues |
Genevisiblei | P63087, MM |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR037981, PPP1CC IPR006186, Ser/Thr-sp_prot-phosphatase IPR031675, STPPase_N |
PANTHERi | PTHR11668:SF204, PTHR11668:SF204, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit PF16891, STPPase_N, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PP1G_MOUSE | |
Accessioni | P63087Primary (citable) accession number: P63087 Secondary accession number(s): O09186 Q64679 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2004 |
Last sequence update: | September 13, 2004 | |
Last modified: | February 10, 2021 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries