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Protein

Gamma-aminobutyric acid receptor subunit beta-3

Gene

Gabrb3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225AgonistBy similarity1

GO - Molecular functioni

  • AP-2 adaptor complex binding Source: RGD
  • chloride channel activity Source: RGD
  • GABA-A receptor activity Source: RGD
  • GABA-gated chloride ion channel activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionChloride channel, Ion channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport
LigandChloride

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit beta-3
Alternative name(s):
GABA(A) receptor subunit beta-3
Gene namesi
Name:Gabrb3
Synonyms:Gabrb-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2651 Gabrb3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 245ExtracellularBy similarityAdd BLAST220
Transmembranei246 – 267HelicalBy similarityAdd BLAST22
Topological domaini268 – 270CytoplasmicBy similarity3
Transmembranei271 – 293HelicalBy similarityAdd BLAST23
Topological domaini294 – 304ExtracellularBy similarityAdd BLAST11
Transmembranei305 – 327HelicalBy similarityAdd BLAST23
Topological domaini328 – 450CytoplasmicBy similarityAdd BLAST123
Transmembranei451 – 472HelicalBy similarityAdd BLAST22

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3883322

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000046426 – 473Gamma-aminobutyric acid receptor subunit beta-3Add BLAST448

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi105N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi161 ↔ 175By similarity
Glycosylationi174N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP63079
PRIDEiP63079

Expressioni

Tissue specificityi

Expressed in brain (at protein level).1 Publication

Interactioni

Subunit structurei

Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains (PubMed:2548852, PubMed:2540033, PubMed:9092594). Can form functional homopentamers (in vitro) (By similarity). Interacts with UBQLN1 (PubMed:11528422). May interact with KIF21B (PubMed:25172774). Identified in a complex of 720 kDa composed of LHFPL4, NLGN2, GABRA1, GABRB2, GABRG2 and GABRB3 (PubMed:28279354). Interacts with LHFPL4 (By similarity).By similarity6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Arfgef2Q7TSU13EBI-6257937,EBI-6257913

Protein-protein interaction databases

ComplexPortaliCPX-280 GABA-A receptor, alpha-4/beta-3/delta
CPX-405 GABA-A receptor, alpha-6/beta-3/gamma-2
CPX-406 GABA-A receptor, alpha-6/beta-3/delta
CPX-409 GABA-A receptor, alpha-3/beta-3/gamma-2
CPX-410 GABA-A receptor, alpha-1/beta-3/gamma-2
CPX-411 GABA-A receptor, alpha-5/beta-3/gamma-2
CPX-412 GABA-A receptor, alpha-2/beta-3/gamma-2
DIPiDIP-60770N
IntActiP63079, 5 interactors
STRINGi10116.ENSRNOP00000061969

Chemistry databases

BindingDBiP63079

Structurei

3D structure databases

ProteinModelPortaliP63079
SMRiP63079
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 122Agonist bindingBy similarity3
Regioni180 – 182Agonist bindingBy similarity3
Regioni290 – 311Allosteric effector bindingBy similarityAdd BLAST22

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3643 Eukaryota
ENOG410XPWH LUCA
HOGENOMiHOG000231335
HOVERGENiHBG051707
InParanoidiP63079
KOiK05181
PhylomeDBiP63079

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006028 GABAA/Glycine_rcpt
IPR002289 GABAAb_rcpt
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR01160 GABAARBETA
PR00253 GABAARECEPTR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

P63079-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MWGFAGGRLF GIFSAPVLVA VVCCAQSVND PGNMSFVKET VDKLLKGYDI
60 70 80 90 100
RLRPDFGGPP VCVGMNIDIA SIDMVSEVNM DYTLTMYFQQ YWRDKRLAYS
110 120 130 140 150
GIPLNLTLDN RVADQLWVPD TYFLNDKKSF VHGVTVKNRM IRLHPDGTVL
160 170 180 190 200
YGLRITTTAA CMMDLRRYPL DEQNCTLEIE SYGYTTDDIE FYWRGGDKAV
210 220 230 240 250
TGVERIELPQ FSIVEHRLVS RNVVFATGAY PRLSLSFRLK RNIGYFILQT
260 270 280 290 300
YMPSILITIL SWVSFWINYD ASAARVALGI TTVLTMTTIN THLRETLPKI
310 320 330 340 350
PYVKAIDMYL MGCFVFVFLA LLEYAFVNYI FFGRGPQRQK KLAEKTAKAK
360 370 380 390 400
NDRSKSEINR VDAHGNILLA PMDVHNEMNE VAGSVGDTRN SAISFDNSGI
410 420 430 440 450
QYRKQSMPKE GHGRYMGDRS IPHKKTHLRR RSSQLKIKIP DLTDVNAIDR
460 470
WSRIVFPFTF SLFNLVYWLY YVN
Length:473
Mass (Da):54,166
Last modified:September 13, 2004 - v1
Checksum:i6A5ABD8A9143FE45
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2K0J9A0A0G2K0J9_RAT
Gamma-aminobutyric acid receptor su...
Gabrb3
456Annotation score:
A0A0G2K5D4A0A0G2K5D4_RAT
Gamma-aminobutyric acid receptor su...
Gabrb3
445Annotation score:
A0A0G2JWR9A0A0G2JWR9_RAT
Gamma-aminobutyric acid receptor su...
Gabrb3
26Annotation score:
A0A0G2JXV3A0A0G2JXV3_RAT
Gamma-aminobutyric acid receptor su...
Gabrb3
24Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256L → M in CAA33495 (PubMed:2548852).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15468 mRNA Translation: CAA33495.1
L04310 Genomic DNA Translation: AAA41180.1
PIRiS04466
RefSeqiNP_058761.1, NM_017065.1
UniGeneiRn.233948

Genome annotation databases

GeneIDi24922
KEGGirno:24922
UCSCiRGD:2651 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15468 mRNA Translation: CAA33495.1
L04310 Genomic DNA Translation: AAA41180.1
PIRiS04466
RefSeqiNP_058761.1, NM_017065.1
UniGeneiRn.233948

3D structure databases

ProteinModelPortaliP63079
SMRiP63079
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-280 GABA-A receptor, alpha-4/beta-3/delta
CPX-405 GABA-A receptor, alpha-6/beta-3/gamma-2
CPX-406 GABA-A receptor, alpha-6/beta-3/delta
CPX-409 GABA-A receptor, alpha-3/beta-3/gamma-2
CPX-410 GABA-A receptor, alpha-1/beta-3/gamma-2
CPX-411 GABA-A receptor, alpha-5/beta-3/gamma-2
CPX-412 GABA-A receptor, alpha-2/beta-3/gamma-2
DIPiDIP-60770N
IntActiP63079, 5 interactors
STRINGi10116.ENSRNOP00000061969

Chemistry databases

BindingDBiP63079
ChEMBLiCHEMBL3883322

Proteomic databases

PaxDbiP63079
PRIDEiP63079

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24922
KEGGirno:24922
UCSCiRGD:2651 rat

Organism-specific databases

CTDi2562
RGDi2651 Gabrb3

Phylogenomic databases

eggNOGiKOG3643 Eukaryota
ENOG410XPWH LUCA
HOGENOMiHOG000231335
HOVERGENiHBG051707
InParanoidiP63079
KOiK05181
PhylomeDBiP63079

Miscellaneous databases

PROiPR:P63079

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006028 GABAA/Glycine_rcpt
IPR002289 GABAAb_rcpt
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR01160 GABAARBETA
PR00253 GABAARECEPTR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGBRB3_RAT
AccessioniPrimary (citable) accession number: P63079
Secondary accession number(s): P15433
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: November 7, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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