UniProtKB - P63074 (IF4E_RAT)
Eukaryotic translation initiation factor 4E
Eif4e
Functioni
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (PubMed:7939721).
In addition to its role in translation initiation, also acts as a regulator of translation and stability in the cytoplasm (PubMed:8558852).
Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression: in the complex, EIF4E mediates the binding to the mRNA cap. Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity).
In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation (By similarity).
May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development (PubMed:8558852).
By similarity2 PublicationsGO - Molecular functioni
- DNA-binding transcription factor binding Source: AgBase
- enzyme binding Source: AgBase
- eukaryotic initiation factor 4G binding Source: RGD
- RNA 7-methylguanosine cap binding Source: UniProtKB
- translation initiation factor activity Source: UniProtKB
- translation regulator activity Source: RGD
GO - Biological processi
- behavioral fear response Source: RGD
- cellular response to dexamethasone stimulus Source: RGD
- G1/S transition of mitotic cell cycle Source: RGD
- lung development Source: RGD
- negative regulation of neuron differentiation Source: RGD
- negative regulation of translation Source: RGD
- positive regulation of mitotic cell cycle Source: RGD
- regulation of translation Source: UniProtKB
- regulation of translation at postsynapse, modulating synaptic transmission Source: RGD
- stem cell population maintenance Source: RGD
Keywordsi
Molecular function | Initiation factor, RNA-binding |
Biological process | Protein biosynthesis, Translation regulation |
Enzyme and pathway databases
Reactomei | R-RNO-1169408, ISG15 antiviral mechanism R-RNO-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-RNO-159227, Transport of the SLBP independent Mature mRNA R-RNO-159230, Transport of the SLBP Dependant Mature mRNA R-RNO-159231, Transport of Mature mRNA Derived from an Intronless Transcript R-RNO-166208, mTORC1-mediated signalling R-RNO-429947, Deadenylation of mRNA R-RNO-72649, Translation initiation complex formation R-RNO-72662, Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-RNO-72702, Ribosomal scanning and start codon recognition R-RNO-72706, GTP hydrolysis and joining of the 60S ribosomal subunit |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic translation initiation factor 4EShort name: eIF-4E Short name: eIF4E Alternative name(s): eIF-4F 25 kDa subunit mRNA cap-binding protein |
Gene namesi | Name:Eif4e |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 69647, Eif4e |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- P-body By similarity
- Stress granule By similarity
Note: Interaction with EIF4ENIF1/4E-T is required for localization to processing bodies (P-bodies). Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism.By similarity
Cytosol
- cytosol Source: UniProtKB
- postsynaptic cytosol Source: SynGO
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- chromatoid body Source: RGD
- cytoplasm Source: RGD
- cytoplasmic stress granule Source: UniProtKB
- eukaryotic translation initiation factor 4F complex Source: RGD
- glutamatergic synapse Source: SynGO
- mRNA cap binding complex Source: UniProtKB
- P-body Source: UniProtKB
- perinuclear region of cytoplasm Source: AgBase
- postsynapse Source: SynGO
- protein-containing complex Source: RGD
- RISC complex Source: RGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000193637 | 2 – 217 | Eukaryotic translation initiation factor 4EAdd BLAST | 216 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 22 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 209 | Phosphoserine; by PKC and MKNK2By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P63074 |
PaxDbi | P63074 |
PRIDEi | P63074 |
PTM databases
iPTMneti | P63074 |
PhosphoSitePlusi | P63074 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000052343, Expressed in testis and 21 other tissues |
ExpressionAtlasi | P63074, baseline and differential |
Genevisiblei | P63074, RN |
Interactioni
Subunit structurei
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions (By similarity). It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners (By similarity).
Interacts with EIF4ENIF1/4E-T; promotes recruitment to P-bodies and import into the nucleus. Hypophosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation.
Interacts mutually exclusive with EIF4A1 or EIF4A2 (PubMed:7939721).
Interacts with NGDN and PIWIL2.
Component of the CYFIP1-EIF4E-FMR1 complex composed of CYFIP, EIF4E and FMR1.
Interacts directly with CYFIP1.
Interacts with CLOCK (By similarity). Binds to MKNK2 in nucleus.
Interacts with LIMD1, WTIP and AJUBA.
Interacts with APOBEC3G in an RNA-dependent manner.
Interacts with LARP1.
Interacts with METTL3.
Interacts with RBM24; this interaction prevents EIF4E from binding to p53/TP53 mRNA and inhibits the assembly of translation initiation complex.
Interacts with DDX3X; interaction is direct and in an RNA-independent manner; this interaction enhances EIF4E cap-binding ability and is required for the repression of cap-dependent translation and the increase of IRES-mediated translation. DDX3X competes with EIF4G1 for interaction with EIF4E (By similarity).
Interacts with BTG4 and CNOT7 (By similarity).
By similarity1 PublicationGO - Molecular functioni
- DNA-binding transcription factor binding Source: AgBase
- enzyme binding Source: AgBase
- eukaryotic initiation factor 4G binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 250643, 4 interactors |
IntActi | P63074, 1 interactor |
STRINGi | 10116.ENSRNOP00000063699 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 27 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 37 – 40 | EIF4EBP1/2/3 bindingBy similarity | 4 | |
Regioni | 56 – 57 | N7-methylguanosine 5'-triphosphate group binding; binds m7GTP residue in mRNA capBy similarity | 2 | |
Regioni | 73 – 77 | EIF4EBP1/2/3 bindingBy similarity | 5 | |
Regioni | 102 – 103 | N7-methylguanosine 5'-triphosphate group binding; binds m7GTP residue in mRNA capBy similarity | 2 | |
Regioni | 132 – 139 | EIF4EBP1/2/3 bindingBy similarity | 8 | |
Regioni | 157 – 162 | N7-methylguanosine 5'-triphosphate group binding; binds m7GTP residue in mRNA capBy similarity | 6 | |
Regioni | 205 – 207 | N7-methylguanosine 5'-triphosphate group binding; binds m7GTP residue in mRNA capBy similarity | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 19 | Polar residuesSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1670, Eukaryota |
GeneTreei | ENSGT00940000154194 |
HOGENOMi | CLU_043552_1_1_1 |
InParanoidi | P63074 |
OMAi | WEDAANN |
OrthoDBi | 1394271at2759 |
PhylomeDBi | P63074 |
Family and domain databases
Gene3Di | 3.30.760.10, 1 hit |
InterProi | View protein in InterPro IPR023398, TIF_eIF4e-like IPR001040, TIF_eIF_4E IPR019770, TIF_eIF_4E_CS |
PANTHERi | PTHR11960, PTHR11960, 1 hit |
Pfami | View protein in Pfam PF01652, IF4E, 1 hit |
SUPFAMi | SSF55418, SSF55418, 1 hit |
PROSITEi | View protein in PROSITE PS00813, IF4E, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MATVEPETTP TTNPPPAEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN
60 70 80 90 100
DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP
110 120 130 140 150
MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC
160 170 180 190 200
GAVVNVRAKG DKIAIWTTEC ENRDAVTHIG RVYKERLGLP PKIVIGYQSH
210
ADTATKSGST TKNRFVV
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2Q627 | A0A1W2Q627_RAT | Eukaryotic translation initiation f... | Eif4e | 218 | Annotation score: | ||
A0A8I6AG37 | A0A8I6AG37_RAT | Eukaryotic translation initiation f... | Eif4e rCG_28755 | 185 | Annotation score: | ||
A0A8I6AT12 | A0A8I6AT12_RAT | Eukaryotic translation initiation f... | Eif4e | 211 | Annotation score: | ||
A0A8I6GKW2 | A0A8I6GKW2_RAT | Eukaryotic translation initiation f... | Eif4e | 79 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83399 mRNA Translation: CAA58316.1 BC087001 mRNA Translation: AAH87001.1 |
RefSeqi | NP_446426.1, NM_053974.2 XP_008759710.1, XM_008761488.2 |
Genome annotation databases
Ensembli | ENSRNOT00000093355; ENSRNOP00000076114; ENSRNOG00000052343 |
GeneIDi | 117045 |
KEGGi | rno:117045 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83399 mRNA Translation: CAA58316.1 BC087001 mRNA Translation: AAH87001.1 |
RefSeqi | NP_446426.1, NM_053974.2 XP_008759710.1, XM_008761488.2 |
3D structure databases
AlphaFoldDBi | P63074 |
BMRBi | P63074 |
SMRi | P63074 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 250643, 4 interactors |
IntActi | P63074, 1 interactor |
STRINGi | 10116.ENSRNOP00000063699 |
PTM databases
iPTMneti | P63074 |
PhosphoSitePlusi | P63074 |
Proteomic databases
jPOSTi | P63074 |
PaxDbi | P63074 |
PRIDEi | P63074 |
Genome annotation databases
Ensembli | ENSRNOT00000093355; ENSRNOP00000076114; ENSRNOG00000052343 |
GeneIDi | 117045 |
KEGGi | rno:117045 |
Organism-specific databases
CTDi | 1977 |
RGDi | 69647, Eif4e |
Phylogenomic databases
eggNOGi | KOG1670, Eukaryota |
GeneTreei | ENSGT00940000154194 |
HOGENOMi | CLU_043552_1_1_1 |
InParanoidi | P63074 |
OMAi | WEDAANN |
OrthoDBi | 1394271at2759 |
PhylomeDBi | P63074 |
Enzyme and pathway databases
Reactomei | R-RNO-1169408, ISG15 antiviral mechanism R-RNO-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-RNO-159227, Transport of the SLBP independent Mature mRNA R-RNO-159230, Transport of the SLBP Dependant Mature mRNA R-RNO-159231, Transport of Mature mRNA Derived from an Intronless Transcript R-RNO-166208, mTORC1-mediated signalling R-RNO-429947, Deadenylation of mRNA R-RNO-72649, Translation initiation complex formation R-RNO-72662, Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-RNO-72702, Ribosomal scanning and start codon recognition R-RNO-72706, GTP hydrolysis and joining of the 60S ribosomal subunit |
Miscellaneous databases
PROi | PR:P63074 |
Gene expression databases
Bgeei | ENSRNOG00000052343, Expressed in testis and 21 other tissues |
ExpressionAtlasi | P63074, baseline and differential |
Genevisiblei | P63074, RN |
Family and domain databases
Gene3Di | 3.30.760.10, 1 hit |
InterProi | View protein in InterPro IPR023398, TIF_eIF4e-like IPR001040, TIF_eIF_4E IPR019770, TIF_eIF_4E_CS |
PANTHERi | PTHR11960, PTHR11960, 1 hit |
Pfami | View protein in Pfam PF01652, IF4E, 1 hit |
SUPFAMi | SSF55418, SSF55418, 1 hit |
PROSITEi | View protein in PROSITE PS00813, IF4E, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IF4E_RAT | |
Accessioni | P63074Primary (citable) accession number: P63074 Secondary accession number(s): P20415 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2004 |
Last sequence update: | September 13, 2004 | |
Last modified: | May 25, 2022 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Translation initiation factors
List of translation initiation factor entries - SIMILARITY comments
Index of protein domains and families