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Protein

Vesicle-associated membrane protein 2

Gene

Vamp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane (PubMed:9430681). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei76 – 77(Microbial infection) Cleavage; by C.botulinum neurotoxin type B (BoNT/B, botB)1 Publication2
Sitei76 – 77(Microbial infection) Cleavage; by C.tetani neurotoxin (tetX)1 Publication2

GO - Molecular functioni

  • calcium-dependent protein binding Source: ParkinsonsUK-UCL
  • calmodulin binding Source: MGI
  • identical protein binding Source: MGI
  • ion channel binding Source: MGI
  • myosin binding Source: MGI
  • phospholipid binding Source: MGI
  • protein-containing complex binding Source: MGI
  • protein C-terminus binding Source: MGI
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: MGI
  • syntaxin-1 binding Source: ParkinsonsUK-UCL
  • syntaxin binding Source: UniProtKB

GO - Biological processi

  • calcium ion regulated exocytosis Source: MGI
  • cellular response to insulin stimulus Source: MGI
  • eosinophil degranulation Source: MGI
  • exocytic insertion of neurotransmitter receptor to postsynaptic membrane Source: SynGO
  • Golgi to plasma membrane protein transport Source: MGI
  • long-term synaptic potentiation Source: MGI
  • membrane fusion Source: MGI
  • positive regulation of intracellular protein transport Source: MGI
  • protein-containing complex assembly Source: MGI
  • protein transport Source: MGI
  • regulation of calcium ion-dependent exocytosis Source: ParkinsonsUK-UCL
  • regulation of delayed rectifier potassium channel activity Source: UniProtKB
  • regulation of exocytosis Source: MGI
  • regulation of synaptic vesicle recycling Source: MGI
  • regulation of vesicle-mediated transport Source: MGI
  • response to glucose Source: MGI
  • SNARE complex assembly Source: MGI
  • synaptic vesicle exocytosis Source: MGI
  • vesicle fusion Source: GO_Central
  • vesicle-mediated transport Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-181429 Serotonin Neurotransmitter Release Cycle
R-MMU-181430 Norepinephrine Neurotransmitter Release Cycle
R-MMU-210500 Glutamate Neurotransmitter Release Cycle
R-MMU-212676 Dopamine Neurotransmitter Release Cycle
R-MMU-264642 Acetylcholine Neurotransmitter Release Cycle
R-MMU-421837 Clathrin derived vesicle budding
R-MMU-432720 Lysosome Vesicle Biogenesis
R-MMU-432722 Golgi Associated Vesicle Biogenesis
R-MMU-449836 Other interleukin signaling
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis
R-MMU-888590 GABA synthesis, release, reuptake and degradation

Protein family/group databases

TCDBi1.F.1.1.4 the synaptosomal vesicle fusion pore (svf-pore) family

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 2
Short name:
VAMP-2
Alternative name(s):
Synaptobrevin-2
Gene namesi
Name:Vamp2
Synonyms:Syb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1313277 Vamp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 94CytoplasmicSequence analysisAdd BLAST93
Transmembranei95 – 114Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST20
Topological domaini115 – 116VesicularSequence analysis2

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002067252 – 116Vesicle-associated membrane protein 2Add BLAST115

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1

Post-translational modificationi

Phosphorylated by PRKCZ in vitro and this phosphorylation is increased in the presence of WDFY2.By similarity
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB); 20 hours after treatment of spinal cord cells almost all the protein has been digested (PubMed:10413679). BoNT/B hydrolyzes the 76-Gln-|-Phe-77 bond and inhibits neurotransmitter release (Probable).1 Publication1 Publication
(Microbial infection) Targeted and hydrolyzed by C.tetani toxin (tetX); 20 hours after treatment of spinal cord cells almost all the protein has been digested (PubMed:10413679). Tetanus toxin hydrolyzes the 76-Gln-|-Phe-77 bond and inhibits neurotransmitter release (Probable).1 Publication1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP63044
PaxDbiP63044
PRIDEiP63044

PTM databases

iPTMnetiP63044
PhosphoSitePlusiP63044
SwissPalmiP63044

Expressioni

Gene expression databases

BgeeiENSMUSG00000020894
CleanExiMM_VAMP2
ExpressionAtlasiP63044 baseline and differential
GenevisibleiP63044 MM

Interactioni

Subunit structurei

Interacts (via N-terminus) with KCNB1 (via N-terminus and C-terminus); stimulates the channel inactivation rate of KCNB1 (By similarity). Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Interacts with VAPA and VAPB (By similarity). Interacts with BVES and STX4. Interacts with WDFY2, PRKCZ and PRKCI (PubMed:17313651). Forms a complex with WDFY2 and PRKCZ (PubMed:17313651).By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • calcium-dependent protein binding Source: ParkinsonsUK-UCL
  • calmodulin binding Source: MGI
  • identical protein binding Source: MGI
  • ion channel binding Source: MGI
  • myosin binding Source: MGI
  • protein C-terminus binding Source: MGI
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: MGI
  • syntaxin-1 binding Source: ParkinsonsUK-UCL
  • syntaxin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi204495, 30 interactors
CORUMiP63044
DIPiDIP-29065N
IntActiP63044, 37 interactors
MINTiP63044
STRINGi10090.ENSMUSP00000021273

Structurei

3D structure databases

ProteinModelPortaliP63044
SMRiP63044
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 91v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860 Eukaryota
COG5143 LUCA
GeneTreeiENSGT00550000074449
HOGENOMiHOG000042711
HOVERGENiHBG006675
InParanoidiP63044
KOiK13504
PhylomeDBiP63044
TreeFamiTF313666

Family and domain databases

InterProiView protein in InterPro
IPR001388 Synaptobrevin
IPR016444 Synaptobrevin/VAMP
IPR028717 VAMP2
PANTHERiPTHR21136:SF91 PTHR21136:SF91, 1 hit
PfamiView protein in Pfam
PF00957 Synaptobrevin, 1 hit
PIRSFiPIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS00417 SYNAPTOBREVIN, 1 hit
PS50892 V_SNARE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63044-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATAATVPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV
60 70 80 90 100
DKVLERDQKL SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG
110
VICAIILIII IVYFST
Length:116
Mass (Da):12,691
Last modified:January 23, 2007 - v2
Checksum:i4A0D0D56B5409D0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60150 mRNA Translation: AAB03463.1
AK090178 mRNA Translation: BAC41125.1
BC055105 mRNA Translation: AAH55105.1
CCDSiCCDS24881.1
RefSeqiNP_033523.1, NM_009497.3
UniGeneiMm.28643

Genome annotation databases

EnsembliENSMUST00000021273; ENSMUSP00000021273; ENSMUSG00000020894
GeneIDi22318
KEGGimmu:22318
UCSCiuc007jpe.1 mouse

Similar proteinsi

Entry informationi

Entry nameiVAMP2_MOUSE
AccessioniPrimary (citable) accession number: P63044
Secondary accession number(s): Q64357
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: July 18, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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