Vamp3

Functionⁱ

SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Siteⁱ	44 – 45	(Microbial infection) Cleavage; by C.botulinum neurotoxin type F (BoNT/F, botF)1 Publication	2
Siteⁱ	46 – 47	(Microbial infection) Cleavage; by C.botulinum neurotoxin type D (BoNT/D, botD)1 Publication	2
Siteⁱ	63 – 64	(Microbial infection) Cleavage; by C.tetani toxin (tetX)1 Publication	2

GO - Molecular functionⁱ

SNAP receptor activity Source: GO_Central
SNARE binding
Source: RGD
Inferred from physical interactionⁱ
- 7698987
syntaxin-1 binding
Source: RGD
Inferred from physical interactionⁱ
- 7698987

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

calcium ion regulated exocytosis Source: Ensembl
cellular response to interferon-gamma Source: Ensembl
exocytosis Source: GO_Central
Golgi to plasma membrane protein transport Source: Ensembl
negative regulation of secretion by cell Source: Ensembl
positive regulation of receptor recycling Source: UniProtKB
protein-containing complex assembly
Source: RGD
Inferred from direct assayⁱ
- 10051443
retrograde transport, endosome to Golgi Source: UniProtKB
SNARE complex assembly Source: Ensembl
substrate adhesion-dependent cell spreading Source: UniProtKB
vesicle fusion Source: GO_Central
vesicle-mediated transport Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Protein transport, Transport

Biological process

Protein transport, Transport

Enzyme and pathway databases

Reactomeⁱ	R-RNO-1236974 ER-Phagosome pathway R-RNO-6811440 Retrograde transport at the Trans-Golgi-Network R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis R-RNO-8856828 Clathrin-mediated endocytosis

Reactomeⁱ

R-RNO-1236974 ER-Phagosome pathway
R-RNO-6811440 Retrograde transport at the Trans-Golgi-Network
R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828 Clathrin-mediated endocytosis

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Vesicle-associated membrane protein 3 Short name: VAMP-3 Alternative name(s): Cellubrevin Short name: CEB Synaptobrevin-3
Gene namesⁱ	Name:Vamp3 Synonyms:Syb3
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 5

Organism-specific databases

Rat genome database More...RGDⁱ	61880 Vamp3

RGDⁱ

61880 Vamp3

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 81	CytoplasmicSequence analysisAdd BLAST	81
Transmembraneⁱ	82 – 102	Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST	21
Topological domainⁱ	103	VesicularSequence analysis	1

Keywords - Cellular componentⁱ

Cell junction, Membrane, Synapse, Synaptosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000206730	1 – 103	Vesicle-associated membrane protein 3Add BLAST		103

Post-translational modificationⁱ

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which hydrolyzes the 46-Lys-|-Leu-47 bond and probably inhibits neurotransmitter release (PubMed:8175689).1 Publication

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which hydrolyzes the 45-Gln-|-Lys-46 bond and probably inhibits neurotransmitter release (PubMed:8175689).1 Publication

(Microbial infection) Targeted and hydrolyzed by C.tetani toxin (tetX) which hydrolyzes the 63-Gln-|-Phe-64 bond and probably inhibits neurotransmitter release (PubMed:8175689).1 Publication

Proteomic databases

PaxDbⁱ	P63025
PRIDEⁱ	P63025

PTM databases

iPTMnetⁱ	P63025
PhosphoSitePlusⁱ	P63025
SwissPalmⁱ	P63025

Expressionⁱ

Tissue specificityⁱ

Ubiquitous.

Gene expression databases

Bgeeⁱ	ENSRNOG00000030055
Genevisibleⁱ	P63025 RN

Interactionⁱ

Subunit structureⁱ

Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with BCAP31; involved in VAMP3 export from the endoplasmic reticulum (By similarity). Interacts with BAIAP3; this interaction is increased in the presence of calcium (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Bves	Q9ES83	3	EBI-7705696,EBI-7705661	From Mus musculus.

With

Entry

#Exp.

IntAct

Notes

Bves

Q9ES83

3

EBI-7705696,EBI-7705661

From Mus musculus.

GO - Molecular functionⁱ

SNAP receptor activity Source: GO_Central
SNARE binding
Source: RGD
Inferred from physical interactionⁱ
- 7698987
syntaxin-1 binding
Source: RGD
Inferred from physical interactionⁱ
- 7698987

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	248164, 1 interactor
CORUMⁱ	P63025
IntActⁱ	P63025, 3 interactors
Molecular INTeraction database More...MINTⁱ	P63025
STRINGⁱ	10116.ENSRNOP00000048364

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Helixⁱ	15 – 75	Combined sources		61

3D structure databases

ProteinModelPortalⁱ	P63025
SMRⁱ	P63025
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	18 – 78	v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST		61

Sequence similaritiesⁱ

Belongs to the synaptobrevin family.Curated

Keywords - Domainⁱ

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG0860 Eukaryota COG5143 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074449
HOGENOMⁱ	HOG000042711
HOVERGENⁱ	HBG006675
InParanoidⁱ	P63025
KEGG Orthology (KO) More...KOⁱ	K13505
OMAⁱ	IIIWACS
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0MPE
PhylomeDBⁱ	P63025
TreeFamⁱ	TF313666

Family and domain databases

InterProⁱ	View protein in InterPro IPR001388 Synaptobrevin IPR016444 Synaptobrevin/VAMP
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00957 Synaptobrevin, 1 hit
PIRSFⁱ	PIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSⁱ	PR00219 SYNAPTOBREVN
PROSITEⁱ	View protein in PROSITE PS00417 SYNAPTOBREVIN, 1 hit PS50892 V_SNARE, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P63025-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSTGVPSGSS AATGSNRRLQ QTQNQVDEVV DIMRVNVDKV LERDQKLSEL 
        60         70         80         90        100
DDRADALQAG ASQFETSAAK LKRKYWWKNC KMWAIGISVL VIIVIIIIVW 

CVS

Length:103

Mass (Da):11,480

Last modified:August 31, 2004 - v1

Checksum:ⁱEB502BBE5D0F5981

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	S63830 mRNA Translation: AAB27554.1 BC088119 mRNA Translation: AAH88119.1
PIRⁱ	S35077
NCBI Reference Sequences More...RefSeqⁱ	NP_476438.1, NM_057097.2
UniGeneⁱ	Rn.219999

Genome annotation databases

Ensemblⁱ	ENSRNOT00000051554; ENSRNOP00000048364; ENSRNOG00000030055
GeneIDⁱ	29528
KEGGⁱ	rno:29528
UCSC genome browser More...UCSCⁱ	RGD:61880 rat

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
P63025	Vesicle-associated membrane protein 3		MOUSE		103
UPI000C87C463		PTEVA		125
UPI0007672018		MYODS		120
UPI00076635BA		PANTA		113
UPI00072DBD21		ACIJB		113
+17

Protein	Similar proteins		Species	Score	Length
P63025	Vesicle-associated membrane protein 3		MOUSE		103
UPI000C87C463		PTEVA		125
UPI0007672018		MYODS		120
UPI00072DBD21		ACIJB		113
UPI00076635BA		PANTA		113
+77

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	S63830 mRNA Translation: AAB27554.1 BC088119 mRNA Translation: AAH88119.1
PIRⁱ	S35077
NCBI Reference Sequences More...RefSeqⁱ	NP_476438.1, NM_057097.2
UniGeneⁱ	Rn.219999

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5KJ7	X-ray	3.50	A/G	14-76	[»]
	5KJ8	X-ray	4.10	A/G	14-76	[»]
ProteinModelPortalⁱ	P63025
SMRⁱ	P63025
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	248164, 1 interactor
CORUMⁱ	P63025
IntActⁱ	P63025, 3 interactors
Molecular INTeraction database More...MINTⁱ	P63025
STRINGⁱ	10116.ENSRNOP00000048364

PTM databases

iPTMnetⁱ	P63025
PhosphoSitePlusⁱ	P63025
SwissPalmⁱ	P63025

Proteomic databases

PaxDbⁱ	P63025
PRIDEⁱ	P63025

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSRNOT00000051554; ENSRNOP00000048364; ENSRNOG00000030055
GeneIDⁱ	29528
KEGGⁱ	rno:29528
UCSC genome browser More...UCSCⁱ	RGD:61880 rat

Organism-specific databases

CTDⁱ	9341
Rat genome database More...RGDⁱ	61880 Vamp3

Phylogenomic databases

eggNOGⁱ	KOG0860 Eukaryota COG5143 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074449
HOGENOMⁱ	HOG000042711
HOVERGENⁱ	HBG006675
InParanoidⁱ	P63025
KEGG Orthology (KO) More...KOⁱ	K13505
OMAⁱ	IIIWACS
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0MPE
PhylomeDBⁱ	P63025
TreeFamⁱ	TF313666

Enzyme and pathway databases

Reactomeⁱ	R-RNO-1236974 ER-Phagosome pathway R-RNO-6811440 Retrograde transport at the Trans-Golgi-Network R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis R-RNO-8856828 Clathrin-mediated endocytosis

Reactomeⁱ

R-RNO-1236974 ER-Phagosome pathway
R-RNO-6811440 Retrograde transport at the Trans-Golgi-Network
R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P63025

PROⁱ

PR:P63025

Gene expression databases

Bgeeⁱ	ENSRNOG00000030055
Genevisibleⁱ	P63025 RN

Family and domain databases

InterProⁱ	View protein in InterPro IPR001388 Synaptobrevin IPR016444 Synaptobrevin/VAMP
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00957 Synaptobrevin, 1 hit
PIRSFⁱ	PIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSⁱ	PR00219 SYNAPTOBREVN
PROSITEⁱ	View protein in PROSITE PS00417 SYNAPTOBREVIN, 1 hit PS50892 V_SNARE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	VAMP3_RAT
Accessionⁱ	P63025Primary (citable) accession number: P63025 Secondary accession number(s): Q64271
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
	Last sequence update:	August 31, 2004
	Last modified:	July 18, 2018
	This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

UniProtKB

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Supporting data

UniProtKB - P63025 (VAMP3_RAT)

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Vesicle-associated membrane protein 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

Endosome

Plasma Membrane

Other locations

Topology

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ