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Protein

Vesicle-associated membrane protein 3

Gene

Vamp3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei44 – 45(Microbial infection) Cleavage; by C.botulinum neurotoxin type F (BoNT/F, botF)1 Publication2
Sitei46 – 47(Microbial infection) Cleavage; by C.botulinum neurotoxin type D (BoNT/D, botD)1 Publication2
Sitei63 – 64(Microbial infection) Cleavage; by C.tetani toxin (tetX)1 Publication2

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: RGD
  • syntaxin-1 binding Source: RGD

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-1236974 ER-Phagosome pathway
R-RNO-6811440 Retrograde transport at the Trans-Golgi-Network
R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 3
Short name:
VAMP-3
Alternative name(s):
Cellubrevin
Short name:
CEB
Synaptobrevin-3
Gene namesi
Name:Vamp3
Synonyms:Syb3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi61880 Vamp3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 81CytoplasmicSequence analysisAdd BLAST81
Transmembranei82 – 102Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini103VesicularSequence analysis1

Keywords - Cellular componenti

Cell junction, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067301 – 103Vesicle-associated membrane protein 3Add BLAST103

Post-translational modificationi

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which hydrolyzes the 46-Lys-|-Leu-47 bond and probably inhibits neurotransmitter release (PubMed:8175689).1 Publication
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which hydrolyzes the 45-Gln-|-Lys-46 bond and probably inhibits neurotransmitter release (PubMed:8175689).1 Publication
(Microbial infection) Targeted and hydrolyzed by C.tetani toxin (tetX) which hydrolyzes the 63-Gln-|-Phe-64 bond and probably inhibits neurotransmitter release (PubMed:8175689).1 Publication

Proteomic databases

PaxDbiP63025
PRIDEiP63025

PTM databases

iPTMnetiP63025
PhosphoSitePlusiP63025
SwissPalmiP63025

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSRNOG00000030055
GenevisibleiP63025 RN

Interactioni

Subunit structurei

Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with BCAP31; involved in VAMP3 export from the endoplasmic reticulum (By similarity). Interacts with BAIAP3; this interaction is increased in the presence of calcium (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BvesQ9ES833EBI-7705696,EBI-7705661From Mus musculus.

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: RGD
  • syntaxin-1 binding Source: RGD

Protein-protein interaction databases

BioGridi248164, 1 interactor
CORUMiP63025
IntActiP63025, 3 interactors
MINTiP63025
STRINGi10116.ENSRNOP00000048364

Structurei

Secondary structure

1103
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 75Combined sources61

3D structure databases

ProteinModelPortaliP63025
SMRiP63025
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 78v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860 Eukaryota
COG5143 LUCA
GeneTreeiENSGT00550000074449
HOGENOMiHOG000042711
HOVERGENiHBG006675
InParanoidiP63025
KOiK13505
OMAiIIIWACS
OrthoDBiEOG091G0MPE
PhylomeDBiP63025
TreeFamiTF313666

Family and domain databases

InterProiView protein in InterPro
IPR001388 Synaptobrevin
IPR016444 Synaptobrevin/VAMP
PfamiView protein in Pfam
PF00957 Synaptobrevin, 1 hit
PIRSFiPIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS00417 SYNAPTOBREVIN, 1 hit
PS50892 V_SNARE, 1 hit

Sequencei

Sequence statusi: Complete.

P63025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTGVPSGSS AATGSNRRLQ QTQNQVDEVV DIMRVNVDKV LERDQKLSEL
60 70 80 90 100
DDRADALQAG ASQFETSAAK LKRKYWWKNC KMWAIGISVL VIIVIIIIVW

CVS
Length:103
Mass (Da):11,480
Last modified:August 31, 2004 - v1
Checksum:iEB502BBE5D0F5981
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63830 mRNA Translation: AAB27554.1
BC088119 mRNA Translation: AAH88119.1
PIRiS35077
RefSeqiNP_476438.1, NM_057097.2
UniGeneiRn.219999

Genome annotation databases

EnsembliENSRNOT00000051554; ENSRNOP00000048364; ENSRNOG00000030055
GeneIDi29528
KEGGirno:29528
UCSCiRGD:61880 rat

Similar proteinsi

Entry informationi

Entry nameiVAMP3_RAT
AccessioniPrimary (citable) accession number: P63025
Secondary accession number(s): Q64271
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: July 18, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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