UniProtKB - P63025 (VAMP3_RAT)
Protein
Vesicle-associated membrane protein 3
Gene
Vamp3
Organism
Rattus norvegicus (Rat)
Status
Functioni
SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 44 – 45 | (Microbial infection) Cleavage; by C.botulinum neurotoxin type F (BoNT/F, botF)1 Publication | 2 | |
Sitei | 46 – 47 | (Microbial infection) Cleavage; by C.botulinum neurotoxin type D (BoNT/D, botD)1 Publication | 2 | |
Sitei | 63 – 64 | (Microbial infection) Cleavage; by C.tetani toxin (tetX)1 Publication | 2 |
GO - Molecular functioni
- SNAP receptor activity Source: GO_Central
- SNARE binding Source: RGD
- syntaxin-1 binding Source: RGD
- syntaxin binding Source: GO_Central
GO - Biological processi
- calcium-ion regulated exocytosis Source: RGD
- cellular response to interferon-gamma Source: RGD
- Golgi to plasma membrane protein transport Source: RGD
- negative regulation of secretion by cell Source: RGD
- positive regulation of receptor recycling Source: UniProtKB
- protein-containing complex assembly Source: RGD
- retrograde transport, endosome to Golgi Source: UniProtKB
- SNARE complex assembly Source: RGD
- substrate adhesion-dependent cell spreading Source: UniProtKB
- vesicle fusion Source: GO_Central
- vesicle-mediated transport Source: UniProtKB
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-6811440, Retrograde transport at the Trans-Golgi-Network R-RNO-8856825, Cargo recognition for clathrin-mediated endocytosis R-RNO-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Vesicle-associated membrane protein 3Short name: VAMP-3 Alternative name(s): Cellubrevin Short name: CEB Synaptobrevin-3 |
Gene namesi | Name:Vamp3 Synonyms:Syb3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61880, Vamp3 |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass type IV membrane protein Curated
- synaptosome By similarity
Cytosol
- cytosol Source: RGD
Endosome
- early endosome Source: RGD
- recycling endosome Source: RGD
Plasma Membrane
- apical plasma membrane Source: RGD
- plasma membrane Source: RGD
Other locations
- cell surface Source: RGD
- clathrin-coated vesicle Source: RGD
- clathrin-coated vesicle membrane Source: RGD
- cytoplasmic vesicle Source: RGD
- integral component of synaptic vesicle membrane Source: SynGO
- intracellular membrane-bounded organelle Source: RGD
- intracellular organelle Source: RGD
- intracellular vesicle Source: CACAO
- neuron projection Source: UniProtKB-KW
- perinuclear region of cytoplasm Source: RGD
- phagocytic vesicle Source: RGD
- secretory granule Source: RGD
- SNARE complex Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 81 | CytoplasmicSequence analysisAdd BLAST | 81 | |
Transmembranei | 82 – 102 | Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 103 | VesicularSequence analysis | 1 |
Keywords - Cellular componenti
Cell junction, Membrane, Synapse, SynaptosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206730 | 1 – 103 | Vesicle-associated membrane protein 3Add BLAST | 103 |
Post-translational modificationi
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which hydrolyzes the 46-Lys-|-Leu-47 bond and probably inhibits neurotransmitter release (PubMed:8175689).1 Publication
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which hydrolyzes the 45-Gln-|-Lys-46 bond and probably inhibits neurotransmitter release (PubMed:8175689).1 Publication
(Microbial infection) Targeted and hydrolyzed by C.tetani toxin (tetX) which hydrolyzes the 63-Gln-|-Phe-64 bond and probably inhibits neurotransmitter release (PubMed:8175689).1 Publication
Proteomic databases
jPOSTi | P63025 |
PaxDbi | P63025 |
PRIDEi | P63025 |
PTM databases
iPTMneti | P63025 |
PhosphoSitePlusi | P63025 |
SwissPalmi | P63025 |
Expressioni
Tissue specificityi
Ubiquitous.
Gene expression databases
Bgeei | ENSRNOG00000030055, Expressed in duodenum and 21 other tissues |
Genevisiblei | P63025, RN |
Interactioni
Subunit structurei
Interacts with BVES (via the C-terminus cytoplasmic tail).
Interacts with BCAP31; involved in VAMP3 export from the endoplasmic reticulum (By similarity).
Interacts with BAIAP3; this interaction is increased in the presence of calcium (By similarity).
Interacts with PICALM (By similarity).
By similarityBinary interactionsi
P63025
With | #Exp. | IntAct |
---|---|---|
Bves [Q9ES83] from Mus musculus. | 3 | EBI-7705696,EBI-7705661 |
GO - Molecular functioni
- SNARE binding Source: RGD
- syntaxin-1 binding Source: RGD
- syntaxin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 248164, 2 interactors |
CORUMi | P63025 |
IntActi | P63025, 5 interactors |
MINTi | P63025 |
STRINGi | 10116.ENSRNOP00000048364 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P63025 |
SMRi | P63025 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 18 – 78 | v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST | 61 |
Sequence similaritiesi
Belongs to the synaptobrevin family.Curated
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0860, Eukaryota |
GeneTreei | ENSGT00940000158192 |
HOGENOMi | CLU_064620_4_1_1 |
InParanoidi | P63025 |
OMAi | FENHART |
OrthoDBi | 1614157at2759 |
PhylomeDBi | P63025 |
TreeFami | TF313666 |
Family and domain databases
InterProi | View protein in InterPro IPR001388, Synaptobrevin IPR016444, Synaptobrevin/VAMP IPR042855, V_SNARE_CC |
PANTHERi | PTHR45701, PTHR45701, 1 hit |
Pfami | View protein in Pfam PF00957, Synaptobrevin, 1 hit |
PIRSFi | PIRSF005409, Synaptobrevin_euk, 1 hit |
PRINTSi | PR00219, SYNAPTOBREVN |
PROSITEi | View protein in PROSITE PS00417, SYNAPTOBREVIN, 1 hit PS50892, V_SNARE, 1 hit |
i Sequence
Sequence statusi: Complete.
P63025-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTGVPSGSS AATGSNRRLQ QTQNQVDEVV DIMRVNVDKV LERDQKLSEL
60 70 80 90 100
DDRADALQAG ASQFETSAAK LKRKYWWKNC KMWAIGISVL VIIVIIIIVW
CVS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S63830 mRNA Translation: AAB27554.1 BC088119 mRNA Translation: AAH88119.1 |
PIRi | S35077 |
RefSeqi | NP_476438.1, NM_057097.2 |
Genome annotation databases
Ensembli | ENSRNOT00000051554; ENSRNOP00000048364; ENSRNOG00000030055 |
GeneIDi | 29528 |
KEGGi | rno:29528 |
UCSCi | RGD:61880, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S63830 mRNA Translation: AAB27554.1 BC088119 mRNA Translation: AAH88119.1 |
PIRi | S35077 |
RefSeqi | NP_476438.1, NM_057097.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5KJ7 | X-ray | 3.50 | A/G | 14-76 | [»] | |
5KJ8 | X-ray | 4.10 | A/G | 14-76 | [»] | |
BMRBi | P63025 | |||||
SMRi | P63025 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 248164, 2 interactors |
CORUMi | P63025 |
IntActi | P63025, 5 interactors |
MINTi | P63025 |
STRINGi | 10116.ENSRNOP00000048364 |
PTM databases
iPTMneti | P63025 |
PhosphoSitePlusi | P63025 |
SwissPalmi | P63025 |
Proteomic databases
jPOSTi | P63025 |
PaxDbi | P63025 |
PRIDEi | P63025 |
Genome annotation databases
Ensembli | ENSRNOT00000051554; ENSRNOP00000048364; ENSRNOG00000030055 |
GeneIDi | 29528 |
KEGGi | rno:29528 |
UCSCi | RGD:61880, rat |
Organism-specific databases
CTDi | 9341 |
RGDi | 61880, Vamp3 |
Phylogenomic databases
eggNOGi | KOG0860, Eukaryota |
GeneTreei | ENSGT00940000158192 |
HOGENOMi | CLU_064620_4_1_1 |
InParanoidi | P63025 |
OMAi | FENHART |
OrthoDBi | 1614157at2759 |
PhylomeDBi | P63025 |
TreeFami | TF313666 |
Enzyme and pathway databases
Reactomei | R-RNO-6811440, Retrograde transport at the Trans-Golgi-Network R-RNO-8856825, Cargo recognition for clathrin-mediated endocytosis R-RNO-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
PROi | PR:P63025 |
Gene expression databases
Bgeei | ENSRNOG00000030055, Expressed in duodenum and 21 other tissues |
Genevisiblei | P63025, RN |
Family and domain databases
InterProi | View protein in InterPro IPR001388, Synaptobrevin IPR016444, Synaptobrevin/VAMP IPR042855, V_SNARE_CC |
PANTHERi | PTHR45701, PTHR45701, 1 hit |
Pfami | View protein in Pfam PF00957, Synaptobrevin, 1 hit |
PIRSFi | PIRSF005409, Synaptobrevin_euk, 1 hit |
PRINTSi | PR00219, SYNAPTOBREVN |
PROSITEi | View protein in PROSITE PS00417, SYNAPTOBREVIN, 1 hit PS50892, V_SNARE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VAMP3_RAT | |
Accessioni | P63025Primary (citable) accession number: P63025 Secondary accession number(s): Q64271 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | August 31, 2004 | |
Last modified: | April 7, 2021 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families