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Entry version 130 (12 Aug 2020)
Sequence version 1 (31 Aug 2004)
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Protein

Fe/S biogenesis protein NfuA

Gene

nfuA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in iron-sulfur cluster biogenesis under severe conditions such as iron starvation or oxidative stress. Binds a 4Fe-4S cluster, can transfer this cluster to apoproteins, and thereby intervenes in the maturation of Fe/S proteins. Could also act as a scaffold/chaperone for damaged Fe/S proteins. Required for E.coli to sustain oxidative stress and iron starvation. Also necessary for the use of extracellular DNA as the sole source of carbon and energy.2 Publications

Caution

Was originally thought to be involved in gluconate metabolism and was referred to as GntY.1 Publication

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

[4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster per subunit. The cluster is presumably bound at the interface of two monomers.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi149Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi152Iron-sulfur (4Fe-4S)Sequence analysis1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processStress response
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:G7748-MONOMER
ECOL316407:JW3377-MONOMER
MetaCyc:G7748-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Fe/S biogenesis protein NfuA
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nfuA
Synonyms:gntY1 Publication, yhgI
Ordered Locus Names:b3414, JW3377
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Cells express the stationary phase-specific competition-defective (SPCD) phenotype and are unable to catabolize dsDNA.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi39C → S: No effect on activity in vivo. Still able to bind a Fe/S cluster in vitro. 1 Publication1
Mutagenesisi44C → S: No effect on activity in vivo. 1 Publication1
Mutagenesisi149C → S: Loss of activity in vivo. Still able to bind a Fe/S cluster in vitro. 1 Publication1
Mutagenesisi152C → S: Loss of activity in vivo. Still able to bind a Fe/S cluster in vitro. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002094641 – 191Fe/S biogenesis protein NfuAAdd BLAST191

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P63020

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P63020

PRoteomics IDEntifications database

More...
PRIDEi
P63020

2D gel databases

Two-dimensional polyacrylamide gel electrophoresis database from the Geneva University Hospital

More...
SWISS-2DPAGEi
P63020

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Up-regulated under conditions of iron depletion or oxidative stress, via IscR. Is also induced by heat shock or the presence of misfolded proteins.3 Publications

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

1 Publication

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4261219, 424 interactors
852234, 1 interactor

Database of interacting proteins

More...
DIPi
DIP-48001N

Protein interaction database and analysis system

More...
IntActi
P63020, 13 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b3414

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P63020

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Consits of two domains, an N-terminal domain that resembles the so-called Fe/S A-type scaffold, and a C-terminal domain that shares sequence identity with Nfu-type scaffold proteins. Both domains are important for NfuA to fulfill its function in vivo. The N-terminal domain does not possess the three conserved cysteine residues thought to act as Fe/S cluster ligands in A-type scaffold proteins, but may keep the ability to interact with apoproteins.1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the NfuA family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0316, Bacteria
COG0694, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_094569_0_0_6

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P63020

KEGG Orthology (KO)

More...
KOi
K07400

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P63020

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.60.300.12, 1 hit
3.30.300.130, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_01637, Fe_S_biogen_NfuA, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR017726, Fe/S_biogenesis_protein_NfuA
IPR000361, FeS_biogenesis
IPR034904, FSCA_dom_sf
IPR035903, HesB-like_dom_sf
IPR001075, NIF_FeS_clus_asmbl_NifU_C

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01521, Fe-S_biosyn, 1 hit
PF01106, NifU, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF117916, SSF117916, 1 hit
SSF89360, SSF89360, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR03341, YhgI_GntY, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P63020-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIRISDAAQA HFAKLLANQE EGTQIRVFVI NPGTPNAECG VSYCPPDAVE
60 70 80 90 100
ATDTALKFDL LTAYVDELSA PYLEDAEIDF VTDQLGSQLT LKAPNAKMRK
110 120 130 140 150
VADDAPLMER VEYMLQSQIN PQLAGHGGRV SLMEITEDGY AILQFGGGCN
160 170 180 190
GCSMVDVTLK EGIEKQLLNE FPELKGVRDL TEHQRGEHSY Y
Length:191
Mass (Da):20,998
Last modified:August 31, 2004 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i06874546ADA5A971
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA58212.1
U00096 Genomic DNA Translation: AAC76439.1
AP009048 Genomic DNA Translation: BAE77877.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A65137

NCBI Reference Sequences

More...
RefSeqi
NP_417873.1, NC_000913.3
WP_000619389.1, NZ_STEB01000004.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC76439; AAC76439; b3414
BAE77877; BAE77877; BAE77877

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
52075030
947925

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW3377
eco:b3414

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.3314

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA58212.1
U00096 Genomic DNA Translation: AAC76439.1
AP009048 Genomic DNA Translation: BAE77877.1
PIRiA65137
RefSeqiNP_417873.1, NC_000913.3
WP_000619389.1, NZ_STEB01000004.1

3D structure databases

SMRiP63020
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4261219, 424 interactors
852234, 1 interactor
DIPiDIP-48001N
IntActiP63020, 13 interactors
STRINGi511145.b3414

2D gel databases

SWISS-2DPAGEiP63020

Proteomic databases

jPOSTiP63020
PaxDbiP63020
PRIDEiP63020

Genome annotation databases

EnsemblBacteriaiAAC76439; AAC76439; b3414
BAE77877; BAE77877; BAE77877
GeneIDi52075030
947925
KEGGiecj:JW3377
eco:b3414
PATRICifig|1411691.4.peg.3314

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB2771

Phylogenomic databases

eggNOGiCOG0316, Bacteria
COG0694, Bacteria
HOGENOMiCLU_094569_0_0_6
InParanoidiP63020
KOiK07400
PhylomeDBiP63020

Enzyme and pathway databases

BioCyciEcoCyc:G7748-MONOMER
ECOL316407:JW3377-MONOMER
MetaCyc:G7748-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P63020

Family and domain databases

Gene3Di2.60.300.12, 1 hit
3.30.300.130, 1 hit
HAMAPiMF_01637, Fe_S_biogen_NfuA, 1 hit
InterProiView protein in InterPro
IPR017726, Fe/S_biogenesis_protein_NfuA
IPR000361, FeS_biogenesis
IPR034904, FSCA_dom_sf
IPR035903, HesB-like_dom_sf
IPR001075, NIF_FeS_clus_asmbl_NifU_C
PfamiView protein in Pfam
PF01521, Fe-S_biosyn, 1 hit
PF01106, NifU, 1 hit
SUPFAMiSSF117916, SSF117916, 1 hit
SSF89360, SSF89360, 1 hit
TIGRFAMsiTIGR03341, YhgI_GntY, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNFUA_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P63020
Secondary accession number(s): P46847, Q2M779
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: August 12, 2020
This is version 130 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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