UniProtKB - P63012 (RAB3A_RAT)
Ras-related protein Rab-3A
Rab3a
Functioni
Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (PubMed:21689256).
Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (PubMed:18407218).
Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (PubMed:17149709).
Stimulates insulin secretion through interaction with RIMS2 and RPH3AL effectors in pancreatic beta cells (By similarity).
Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (By similarity).
Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (By similarity).
Plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (PubMed:28057568).
Interacts with MADD (via uDENN domain); the GTP-bound form is preferred for interaction (By similarity).
By similarity4 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 29 – 37 | GTP1 Publication | 9 | |
Nucleotide bindingi | 48 – 54 | GTP1 Publication | 7 | |
Nucleotide bindingi | 77 – 81 | GTP1 Publication | 5 | |
Nucleotide bindingi | 135 – 138 | GTP1 Publication | 4 | |
Nucleotide bindingi | 165 – 167 | GTPBy similarity | 3 |
GO - Molecular functioni
- ATPase activator activity Source: RGD
- ATPase binding Source: RGD
- GDP-dissociation inhibitor binding Source: RGD
- GTPase activity Source: RGD
- GTP binding Source: RGD
- GTP-dependent protein binding Source: RGD
- myosin V binding Source: RGD
- protein C-terminus binding Source: RGD
GO - Biological processi
- acrosomal vesicle exocytosis Source: RGD
- axonogenesis Source: RGD
- calcium-ion regulated exocytosis Source: RGD
- evoked neurotransmitter secretion Source: RGD
- exocytosis Source: RGD
- lung development Source: RGD
- lysosome localization Source: RGD
- maintenance of presynaptic active zone structure Source: RGD
- mitochondrion organization Source: RGD
- neuromuscular synaptic transmission Source: RGD
- plasma membrane repair Source: RGD
- positive regulation of regulated secretory pathway Source: RGD
- post-embryonic development Source: RGD
- protein localization to plasma membrane Source: GO_Central
- protein secretion Source: GO_Central
- Rab protein signal transduction Source: RGD
- regulated exocytosis Source: RGD
- regulation of dopamine secretion Source: CACAO
- regulation of exocytosis Source: RGD
- regulation of plasma membrane repair Source: RGD
- regulation of short-term neuronal synaptic plasticity Source: ParkinsonsUK-UCL
- regulation of synaptic vesicle exocytosis Source: UniProtKB
- regulation of synaptic vesicle fusion to presynaptic active zone membrane Source: ParkinsonsUK-UCL
- regulation of synaptic vesicle priming Source: UniProtKB
- respiratory system process Source: RGD
- response to electrical stimulus Source: RGD
- sensory perception of touch Source: RGD
- synaptic vesicle clustering Source: RGD
- synaptic vesicle exocytosis Source: ParkinsonsUK-UCL
- synaptic vesicle maturation Source: RGD
- synaptic vesicle recycling Source: ParkinsonsUK-UCL
- synaptic vesicle transport Source: RGD
- vesicle docking involved in exocytosis Source: GO_Central
Keywordsi
Biological process | Exocytosis, Protein transport, Transport |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-181429, Serotonin Neurotransmitter Release Cycle R-RNO-181430, Norepinephrine Neurotransmitter Release Cycle R-RNO-210500, Glutamate Neurotransmitter Release Cycle R-RNO-212676, Dopamine Neurotransmitter Release Cycle R-RNO-264642, Acetylcholine Neurotransmitter Release Cycle R-RNO-6798695, Neutrophil degranulation R-RNO-8873719, RAB geranylgeranylation R-RNO-8876198, RAB GEFs exchange GTP for GDP on RABs R-RNO-888590, GABA synthesis, release, reuptake and degradation |
Names & Taxonomyi
Protein namesi | Recommended name: Ras-related protein Rab-3A |
Gene namesi | Name:Rab3a |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3528, Rab3a |
Subcellular locationi
Lysosome
- Lysosome By similarity
Plasma membrane
- Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated
Cytoplasm and Cytosol
- cytosol 2 Publications
Other locations
- secretory vesicle 2 Publications
- axon By similarity
- presynapse By similarity
- postsynapse By similarity
Note: Cycles between a vesicle-associated GTP-bound form and a cytosolic GDP-bound form.1 Publication
Cytosol
- cytosol Source: RGD
Endosome
- endosome Source: RGD
Lysosome
- lysosome Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- acrosomal vesicle Source: RGD
- anchored component of synaptic vesicle membrane Source: SynGO
- axon Source: ParkinsonsUK-UCL
- intracellular organelle Source: RGD
- membrane Source: RGD
- perinuclear region of cytoplasm Source: RGD
- postsynapse Source: UniProtKB
- presynaptic active zone Source: UniProtKB
- protein-containing complex Source: RGD
- secretory granule Source: UniProtKB
- synaptic vesicle Source: UniProtKB
- terminal bouton Source: ParkinsonsUK-UCL
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Lysosome, Membrane, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 36 | T → N: No significant effect on interaction with RAB3IP. 1 Publication | 1 | |
Mutagenesisi | 51 | F → L: Disrupts the interaction with RAB3IP. 1 Publication | 1 | |
Mutagenesisi | 52 | V → A: No significant effect on interaction with RAB3IP. 1 Publication | 1 | |
Mutagenesisi | 55 | V → E: Disrupts the interaction with RAB3IP. 1 Publication | 1 | |
Mutagenesisi | 56 | G → D: Disrupts the interaction with RAB3IP. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121079 | 1 – 220 | Ras-related protein Rab-3AAdd BLAST | 220 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 86 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 188 | PhosphoserineCombined sources | 1 | |
Modified residuei | 190 | PhosphoserineCombined sources | 1 | |
Lipidationi | 218 | S-geranylgeranyl cysteineBy similarity | 1 | |
Modified residuei | 220 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 220 | S-geranylgeranyl cysteineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Methylation, Phosphoprotein, PrenylationProteomic databases
jPOSTi | P63012 |
PaxDbi | P63012 |
PRIDEi | P63012 |
PTM databases
iPTMneti | P63012 |
PhosphoSitePlusi | P63012 |
SwissPalmi | P63012 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000019433, Expressed in brain and 22 other tissues |
Genevisiblei | P63012, RN |
Interactioni
Subunit structurei
Interacts with RIMS1 and RIMS2 (PubMed:10748113) (By similarity).
Interacts with Rabphilin-3A/RPH3A and Rab effector Noc2/RPH3AL (PubMed:10025402) (By similarity).
Interacts with SYTL4 (By similarity).
Interacts with RAB3IP (PubMed:7532276).
Interacts with SGSM1 and SGSM3 (By similarity).
Interacts with SYT1 (PubMed:28057568).
Interacts with MYH9; this interaction is essential for lysosome exocytosis and plasma membrane repair (By similarity).
Interacts with STXBP1; this interaction promotes RAB3A dissociation from the vesicle membrane (PubMed:21689256).
Interacts with SNCA (By similarity).
Interacts with GDI1, GDI2 and CHM; phosphorylation at Thr-86 disrupts these interactions (By similarity).
By similarity5 PublicationsBinary interactionsi
P63012
With | #Exp. | IntAct |
---|---|---|
Rph3a [P47709] | 2 | EBI-440126,EBI-1027524 |
GO - Molecular functioni
- ATPase binding Source: RGD
- GDP-dissociation inhibitor binding Source: RGD
- GTP-dependent protein binding Source: RGD
- myosin V binding Source: RGD
- protein C-terminus binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 247563, 6 interactors |
CORUMi | P63012 |
IntActi | P63012, 6 interactors |
MINTi | P63012 |
STRINGi | 10116.ENSRNOP00000026392 |
Structurei
Secondary structure
3D structure databases
SMRi | P63012 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P63012 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 194 – 220 | DisorderedSequence analysisAdd BLAST | 27 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 51 – 59 | Effector regionBy similarity | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0093, Eukaryota |
GeneTreei | ENSGT00940000158959 |
HOGENOMi | CLU_041217_10_1_1 |
InParanoidi | P63012 |
OMAi | TPPHQDC |
OrthoDBi | 1426655at2759 |
PhylomeDBi | P63012 |
TreeFami | TF313199 |
Family and domain databases
CDDi | cd01865, Rab3, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR037872, Rab3 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MASATDSRYG QKESSDQNFD YMFKILIIGN SSVGKTSFLF RYADDSFTPA
60 70 80 90 100
FVSTVGIDFK VKTIYRNDKR IKLQIWDTAG QERYRTITTA YYRGAMGFIL
110 120 130 140 150
MYDITNEESF NAVQDWSTQI KTYSWDNAQV LLVGNKCDME DERVVSSERG
160 170 180 190 200
RQLADHLGFE FFEASAKDNI NVKQTFERLV DVICEKMSES LDTADPAVTG
210 220
AKQGPQLTDQ QAPPHQDCAC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 196 | P → L in CAA30005 (PubMed:3344209).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06889 mRNA Translation: CAA30005.1 BC087580 mRNA Translation: AAH87580.1 |
PIRi | S01765 |
RefSeqi | NP_037150.2, NM_013018.2 |
Genome annotation databases
Ensembli | ENSRNOT00000026391; ENSRNOP00000026392; ENSRNOG00000019433 |
GeneIDi | 25531 |
KEGGi | rno:25531 |
UCSCi | RGD:3528, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06889 mRNA Translation: CAA30005.1 BC087580 mRNA Translation: AAH87580.1 |
PIRi | S01765 |
RefSeqi | NP_037150.2, NM_013018.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZBD | X-ray | 2.60 | A | 19-217 | [»] | |
3RAB | X-ray | 2.00 | A | 18-186 | [»] | |
SMRi | P63012 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 247563, 6 interactors |
CORUMi | P63012 |
IntActi | P63012, 6 interactors |
MINTi | P63012 |
STRINGi | 10116.ENSRNOP00000026392 |
PTM databases
iPTMneti | P63012 |
PhosphoSitePlusi | P63012 |
SwissPalmi | P63012 |
Proteomic databases
jPOSTi | P63012 |
PaxDbi | P63012 |
PRIDEi | P63012 |
Genome annotation databases
Ensembli | ENSRNOT00000026391; ENSRNOP00000026392; ENSRNOG00000019433 |
GeneIDi | 25531 |
KEGGi | rno:25531 |
UCSCi | RGD:3528, rat |
Organism-specific databases
CTDi | 5864 |
RGDi | 3528, Rab3a |
Phylogenomic databases
eggNOGi | KOG0093, Eukaryota |
GeneTreei | ENSGT00940000158959 |
HOGENOMi | CLU_041217_10_1_1 |
InParanoidi | P63012 |
OMAi | TPPHQDC |
OrthoDBi | 1426655at2759 |
PhylomeDBi | P63012 |
TreeFami | TF313199 |
Enzyme and pathway databases
Reactomei | R-RNO-181429, Serotonin Neurotransmitter Release Cycle R-RNO-181430, Norepinephrine Neurotransmitter Release Cycle R-RNO-210500, Glutamate Neurotransmitter Release Cycle R-RNO-212676, Dopamine Neurotransmitter Release Cycle R-RNO-264642, Acetylcholine Neurotransmitter Release Cycle R-RNO-6798695, Neutrophil degranulation R-RNO-8873719, RAB geranylgeranylation R-RNO-8876198, RAB GEFs exchange GTP for GDP on RABs R-RNO-888590, GABA synthesis, release, reuptake and degradation |
Miscellaneous databases
EvolutionaryTracei | P63012 |
PROi | PR:P63012 |
Gene expression databases
Bgeei | ENSRNOG00000019433, Expressed in brain and 22 other tissues |
Genevisiblei | P63012, RN |
Family and domain databases
CDDi | cd01865, Rab3, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR037872, Rab3 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAB3A_RAT | |
Accessioni | P63012Primary (citable) accession number: P63012 Secondary accession number(s): P05713 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | August 31, 2004 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families