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Protein

60S ribosomal protein L8

Gene

RPL8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the large ribosomal subunit.1 Publication

Miscellaneous

This protein can be partially incorporated into E.coli polysomes in vivo, indicating it can replace the endogenous protein.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-156902 Peptide chain elongation
R-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane
R-HSA-192823 Viral mRNA Translation
R-HSA-2408557 Selenocysteine synthesis
R-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-HSA-72764 Eukaryotic Translation Termination
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L8
Alternative name(s):
Large ribosomal subunit protein uL21 Publication
Gene namesi
Name:RPL8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000161016.15
HGNCiHGNC:10368 RPL8
MIMi604177 gene
neXtProtiNX_P62917

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi209H → A or G: No incorporation into translating E.coli polysomes; ribosomes assembled normally. Significantly reduced translational activity. 1 Publication1

Organism-specific databases

DisGeNETi6132
OpenTargetsiENSG00000161016
PharmGKBiPA34768

Chemistry databases

DrugBankiDB02494 Alpha-Hydroxy-Beta-Phenyl-Propionic Acid
DB07374 Anisomycin
DB08437 Puromycin

Polymorphism and mutation databases

BioMutaiRPL8
DMDMi51702823

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001297432 – 25760S ribosomal protein L8Add BLAST256

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki42Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki149Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei216(3S)-3-hydroxyhistidine1 Publication1
Cross-linki234Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki250Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Hydroxylated on His-216 by RIOX1. The modification is impaired by hypoxia.1 Publication

Keywords - PTMi

Hydroxylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP62917
MaxQBiP62917
PaxDbiP62917
PeptideAtlasiP62917
PRIDEiP62917
ProteomicsDBi57454
TopDownProteomicsiP62917

PTM databases

iPTMnetiP62917
PhosphoSitePlusiP62917
SwissPalmiP62917

Expressioni

Gene expression databases

BgeeiENSG00000161016
CleanExiHS_RPL8
ExpressionAtlasiP62917 baseline and differential
GenevisibleiP62917 HS

Organism-specific databases

HPAiHPA045095
HPA050165

Interactioni

Subunit structurei

Component of the large ribosomal subunit (Probable). Interacts with CRY1 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi112052, 223 interactors
CORUMiP62917
DIPiDIP-37967N
IntActiP62917, 59 interactors
MINTiP62917
STRINGi9606.ENSP00000262584

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CCMX-ray2.51C/D205-239[»]
4CCNX-ray2.23C/D205-239[»]
4CCOX-ray2.30C/D205-224[»]
4UG0electron microscopy-LA1-257[»]
4V6Xelectron microscopy5.00CA1-257[»]
4Y3OX-ray2.20C/D212-222[»]
5AJ0electron microscopy3.50AA1-257[»]
5LKSelectron microscopy3.60LA1-257[»]
5T2Celectron microscopy3.60D1-257[»]
6EK0electron microscopy2.90LA1-257[»]
ProteinModelPortaliP62917
SMRiP62917
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2309 Eukaryota
COG0090 LUCA
GeneTreeiENSGT00390000009907
HOVERGENiHBG079431
InParanoidiP62917
KOiK02938
OMAiHGYIKGV
OrthoDBiEOG091G0K1G
PhylomeDBiP62917
TreeFamiTF300748

Family and domain databases

Gene3Di2.30.30.30, 1 hit
4.10.950.10, 1 hit
HAMAPiMF_01320_A Ribosomal_L2_A, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR022666 Rbsml_prot_L2_RNA-bd_dom
IPR014722 Rib_L2_dom2
IPR002171 Ribosomal_L2
IPR023672 Ribosomal_L2_arc
IPR022669 Ribosomal_L2_C
IPR022671 Ribosomal_L2_CS
IPR014726 Ribosomal_L2_dom3
IPR008991 Translation_prot_SH3-like_sf
PANTHERiPTHR13691 PTHR13691, 1 hit
PfamiView protein in Pfam
PF00181 Ribosomal_L2, 1 hit
PF03947 Ribosomal_L2_C, 1 hit
PIRSFiPIRSF002158 Ribosomal_L2, 1 hit
SMARTiView protein in SMART
SM01383 Ribosomal_L2, 1 hit
SM01382 Ribosomal_L2_C, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
SSF50249 SSF50249, 1 hit
PROSITEiView protein in PROSITE
PS00467 RIBOSOMAL_L2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62917-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH
60 70 80 90 100
DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN
110 120 130 140 150
VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL
160 170 180 190 200
PSGSKKVISS ANRAVVGVVA GGGRIDKPIL KAGRAYHKYK AKRNCWPRVR
210 220 230 240 250
GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA RRTGRLRGTK

TVQEKEN
Length:257
Mass (Da):28,025
Last modified:January 23, 2007 - v2
Checksum:iCE1610449749318B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01965898I → V2 PublicationsCorresponds to variant dbSNP:rs11539893Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28407 mRNA Translation: CAA82248.1
AB061821 Genomic DNA Translation: BAB79459.1
BT007379 mRNA Translation: AAP36043.1
CR457046 mRNA Translation: CAG33327.1
AK289459 mRNA Translation: BAF82148.1
CH471162 Genomic DNA Translation: EAW82048.1
CH471162 Genomic DNA Translation: EAW82051.1
CH471162 Genomic DNA Translation: EAW82052.1
BC000047 mRNA Translation: AAH00047.2
BC000077 mRNA Translation: AAH00077.1
BC012197 mRNA Translation: AAH12197.1
BC013104 mRNA Translation: AAH13104.1
BC093064 mRNA Translation: AAH93064.1
AB007168 Genomic DNA Translation: BAA25829.1
CCDSiCCDS6433.1
PIRiJN0923
RefSeqiNP_000964.1, NM_000973.4
NP_001304700.1, NM_001317771.1
NP_001304711.1, NM_001317782.1
NP_150644.1, NM_033301.2
UniGeneiHs.178551

Genome annotation databases

EnsembliENST00000262584; ENSP00000262584; ENSG00000161016
ENST00000394920; ENSP00000378378; ENSG00000161016
ENST00000528957; ENSP00000433464; ENSG00000161016
GeneIDi6132
KEGGihsa:6132
UCSCiuc003zeb.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRL8_HUMAN
AccessioniPrimary (citable) accession number: P62917
Secondary accession number(s): A8K094
, D3DWN2, P25120, Q567Q7, Q969V7, Q9BWQ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Ribosomal proteins
    Ribosomal proteins families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

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