UniProtKB - P62917 (RL8_HUMAN)
Protein
60S ribosomal protein L8
Gene
RPL8
Organism
Homo sapiens (Human)
Status
Functioni
Component of the large ribosomal subunit.1 Publication
Miscellaneous
This protein can be partially incorporated into E.coli polysomes in vivo, indicating it can replace the endogenous protein.
GO - Molecular functioni
- RNA binding Source: UniProtKB
- rRNA binding Source: UniProtKB-KW
- structural constituent of ribosome Source: GO_Central
GO - Biological processi
- cytoplasmic translation Source: UniProtKB
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: Reactome
- SRP-dependent cotranslational protein targeting to membrane Source: Reactome
- translation Source: ProtInc
- translational initiation Source: Reactome
- viral transcription Source: Reactome
Keywordsi
Molecular function | Ribonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding |
Enzyme and pathway databases
PathwayCommonsi | P62917 |
Reactomei | R-HSA-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-HSA-156902, Peptide chain elongation R-HSA-1799339, SRP-dependent cotranslational protein targeting to membrane R-HSA-192823, Viral mRNA Translation R-HSA-2408557, Selenocysteine synthesis R-HSA-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-HSA-72689, Formation of a pool of free 40S subunits R-HSA-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-HSA-72764, Eukaryotic Translation Termination R-HSA-9010553, Regulation of expression of SLITs and ROBOs R-HSA-9633012, Response of EIF2AK4 (GCN2) to amino acid deficiency R-HSA-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-HSA-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: 60S ribosomal protein L8Alternative name(s): Large ribosomal subunit protein uL21 Publication |
Gene namesi | Name:RPL8 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000161016.15 |
HGNCi | HGNC:10368, RPL8 |
MIMi | 604177, gene |
neXtProti | NX_P62917 |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: Reactome
- cytosolic large ribosomal subunit Source: UniProtKB
Other locations
- focal adhesion Source: UniProtKB
- membrane Source: UniProtKB
- polysomal ribosome Source: UniProtKB
- synapse Source: Ensembl
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 209 | H → A or G: No incorporation into translating E.coli polysomes; ribosomes assembled normally. Significantly reduced translational activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6132 |
OpenTargetsi | ENSG00000161016 |
PharmGKBi | PA34768 |
Miscellaneous databases
Pharosi | P62917, Tbio |
Chemistry databases
DrugBanki | DB02494, (S)-3-phenyllactic acid DB07374, Anisomycin DB08437, Puromycin |
Polymorphism and mutation databases
BioMutai | RPL8 |
DMDMi | 51702823 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000129743 | 2 – 257 | 60S ribosomal protein L8Add BLAST | 256 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 42 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 149 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 216 | (3S)-3-hydroxyhistidine1 Publication | 1 | |
Cross-linki | 234 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 250 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Post-translational modificationi
Hydroxylated on His-216 by RIOX1. The modification is impaired by hypoxia.1 Publication
Keywords - PTMi
Hydroxylation, Isopeptide bond, Ubl conjugationProteomic databases
EPDi | P62917 |
jPOSTi | P62917 |
MassIVEi | P62917 |
MaxQBi | P62917 |
PaxDbi | P62917 |
PeptideAtlasi | P62917 |
PRIDEi | P62917 |
ProteomicsDBi | 57454 |
TopDownProteomicsi | P62917 |
PTM databases
iPTMneti | P62917 |
MetOSitei | P62917 |
PhosphoSitePlusi | P62917 |
SwissPalmi | P62917 |
Expressioni
Gene expression databases
Bgeei | ENSG00000161016, Expressed in muscle tissue and 134 other tissues |
ExpressionAtlasi | P62917, baseline and differential |
Genevisiblei | P62917, HS |
Organism-specific databases
HPAi | ENSG00000161016, Low tissue specificity |
Interactioni
Subunit structurei
Component of the large ribosomal subunit (Probable).
Interacts with CRY1 (By similarity).
By similarity1 PublicationBinary interactionsi
P62917
With | #Exp. | IntAct |
---|---|---|
MAP3K14 [Q99558] | 3 | EBI-438527,EBI-358011 |
Protein-protein interaction databases
BioGRIDi | 112052, 289 interactors |
ComplexPortali | CPX-5183, 60S cytosolic large ribosomal subunit |
CORUMi | P62917 |
DIPi | DIP-37967N |
IntActi | P62917, 121 interactors |
MINTi | P62917 |
STRINGi | 9606.ENSP00000262584 |
Miscellaneous databases
RNActi | P62917, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the universal ribosomal protein uL2 family.Curated
Phylogenomic databases
eggNOGi | KOG2309, Eukaryota |
GeneTreei | ENSGT00940000153244 |
InParanoidi | P62917 |
OMAi | SCIELRP |
OrthoDBi | 1156335at2759 |
PhylomeDBi | P62917 |
TreeFami | TF300748 |
Family and domain databases
Gene3Di | 2.30.30.30, 1 hit 4.10.950.10, 1 hit |
HAMAPi | MF_01320_A, Ribosomal_L2_A, 1 hit |
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR022666, Rbsml_prot_L2_RNA-bd_dom IPR014722, Rib_L2_dom2 IPR002171, Ribosomal_L2 IPR023672, Ribosomal_L2_arc IPR022669, Ribosomal_L2_C IPR022671, Ribosomal_L2_CS IPR014726, Ribosomal_L2_dom3 IPR008991, Translation_prot_SH3-like_sf |
PANTHERi | PTHR13691, PTHR13691, 1 hit |
Pfami | View protein in Pfam PF00181, Ribosomal_L2, 1 hit PF03947, Ribosomal_L2_C, 1 hit |
PIRSFi | PIRSF002158, Ribosomal_L2, 1 hit |
SMARTi | View protein in SMART SM01383, Ribosomal_L2, 1 hit SM01382, Ribosomal_L2_C, 1 hit |
SUPFAMi | SSF50104, SSF50104, 1 hit SSF50249, SSF50249, 1 hit |
PROSITEi | View protein in PROSITE PS00467, RIBOSOMAL_L2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
P62917-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH
60 70 80 90 100
DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN
110 120 130 140 150
VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL
160 170 180 190 200
PSGSKKVISS ANRAVVGVVA GGGRIDKPIL KAGRAYHKYK AKRNCWPRVR
210 220 230 240 250
GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA RRTGRLRGTK
TVQEKEN
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PKU4 | E9PKU4_HUMAN | 60S ribosomal protein L8 | RPL8 | 235 | Annotation score: | ||
E9PP36 | E9PP36_HUMAN | 60S ribosomal protein L8 | RPL8 | 148 | Annotation score: | ||
E9PKZ0 | E9PKZ0_HUMAN | 60S ribosomal protein L8 | RPL8 | 205 | Annotation score: | ||
G3V1A1 | G3V1A1_HUMAN | 60S ribosomal protein L8 | RPL8 hCG_24000 | 167 | Annotation score: | ||
H0YF23 | H0YF23_HUMAN | 60S ribosomal protein L8 | RPL8 | 110 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_019658 | 98 | I → V2 PublicationsCorresponds to variant dbSNP:rs11539893Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z28407 mRNA Translation: CAA82248.1 AB061821 Genomic DNA Translation: BAB79459.1 BT007379 mRNA Translation: AAP36043.1 CR457046 mRNA Translation: CAG33327.1 AK289459 mRNA Translation: BAF82148.1 CH471162 Genomic DNA Translation: EAW82048.1 CH471162 Genomic DNA Translation: EAW82051.1 CH471162 Genomic DNA Translation: EAW82052.1 BC000047 mRNA Translation: AAH00047.2 BC000077 mRNA Translation: AAH00077.1 BC012197 mRNA Translation: AAH12197.1 BC013104 mRNA Translation: AAH13104.1 BC093064 mRNA Translation: AAH93064.1 AB007168 Genomic DNA Translation: BAA25829.1 |
CCDSi | CCDS6433.1 |
PIRi | JN0923 |
RefSeqi | NP_000964.1, NM_000973.4 NP_001304700.1, NM_001317771.1 NP_001304711.1, NM_001317782.1 NP_150644.1, NM_033301.2 |
Genome annotation databases
Ensembli | ENST00000262584; ENSP00000262584; ENSG00000161016 ENST00000394920; ENSP00000378378; ENSG00000161016 ENST00000528957; ENSP00000433464; ENSG00000161016 |
GeneIDi | 6132 |
KEGGi | hsa:6132 |
UCSCi | uc003zeb.4, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z28407 mRNA Translation: CAA82248.1 AB061821 Genomic DNA Translation: BAB79459.1 BT007379 mRNA Translation: AAP36043.1 CR457046 mRNA Translation: CAG33327.1 AK289459 mRNA Translation: BAF82148.1 CH471162 Genomic DNA Translation: EAW82048.1 CH471162 Genomic DNA Translation: EAW82051.1 CH471162 Genomic DNA Translation: EAW82052.1 BC000047 mRNA Translation: AAH00047.2 BC000077 mRNA Translation: AAH00077.1 BC012197 mRNA Translation: AAH12197.1 BC013104 mRNA Translation: AAH13104.1 BC093064 mRNA Translation: AAH93064.1 AB007168 Genomic DNA Translation: BAA25829.1 |
CCDSi | CCDS6433.1 |
PIRi | JN0923 |
RefSeqi | NP_000964.1, NM_000973.4 NP_001304700.1, NM_001317771.1 NP_001304711.1, NM_001317782.1 NP_150644.1, NM_033301.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4CCM | X-ray | 2.51 | C/D | 205-239 | [»] | |
4CCN | X-ray | 2.23 | C/D | 205-239 | [»] | |
4CCO | X-ray | 2.30 | C/D | 205-224 | [»] | |
4UG0 | electron microscopy | - | LA | 1-257 | [»] | |
4V6X | electron microscopy | 5.00 | CA | 1-257 | [»] | |
4Y3O | X-ray | 2.20 | C/D | 212-222 | [»] | |
5AJ0 | electron microscopy | 3.50 | AA | 1-257 | [»] | |
5LKS | electron microscopy | 3.60 | LA | 1-257 | [»] | |
5T2C | electron microscopy | 3.60 | D | 1-257 | [»] | |
6EK0 | electron microscopy | 2.90 | LA | 1-257 | [»] | |
6IP5 | electron microscopy | 3.90 | 1D | 1-257 | [»] | |
6IP6 | electron microscopy | 4.50 | 1D | 1-257 | [»] | |
6IP8 | electron microscopy | 3.90 | 1D | 1-257 | [»] | |
6LQM | electron microscopy | 3.09 | m | 1-257 | [»] | |
6LSR | electron microscopy | 3.13 | m | 1-257 | [»] | |
6LSS | electron microscopy | 3.23 | m | 1-257 | [»] | |
6LU8 | electron microscopy | 3.13 | m | 1-257 | [»] | |
6OLE | electron microscopy | 3.10 | A | 2-253 | [»] | |
6OLF | electron microscopy | 3.90 | A | 2-253 | [»] | |
6OLG | electron microscopy | 3.40 | AA | 1-257 | [»] | |
6OLI | electron microscopy | 3.50 | A | 2-253 | [»] | |
6OLZ | electron microscopy | 3.90 | AA | 2-249 | [»] | |
6OM0 | electron microscopy | 3.10 | A | 2-253 | [»] | |
6OM7 | electron microscopy | 3.70 | A | 2-253 | [»] | |
6QZP | electron microscopy | 2.90 | LA | 2-249 | [»] | |
6W6L | electron microscopy | 3.84 | A | 1-257 | [»] | |
6Y0G | electron microscopy | 3.20 | LA | 1-257 | [»] | |
6Y2L | electron microscopy | 3.00 | LA | 1-257 | [»] | |
6Y57 | electron microscopy | 3.50 | LA | 1-257 | [»] | |
6Y6X | electron microscopy | 2.80 | LA | 2-249 | [»] | |
6Z6L | electron microscopy | 3.00 | LA | 1-257 | [»] | |
6Z6M | electron microscopy | 3.10 | LA | 1-257 | [»] | |
6Z6N | electron microscopy | 2.90 | LA | 1-257 | [»] | |
6ZM7 | electron microscopy | 2.70 | LA | 1-257 | [»] | |
6ZME | electron microscopy | 3.00 | LA | 1-257 | [»] | |
6ZMI | electron microscopy | 2.60 | LA | 1-257 | [»] | |
6ZMO | electron microscopy | 3.10 | LA | 1-257 | [»] | |
SMRi | P62917 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112052, 289 interactors |
ComplexPortali | CPX-5183, 60S cytosolic large ribosomal subunit |
CORUMi | P62917 |
DIPi | DIP-37967N |
IntActi | P62917, 121 interactors |
MINTi | P62917 |
STRINGi | 9606.ENSP00000262584 |
Chemistry databases
DrugBanki | DB02494, (S)-3-phenyllactic acid DB07374, Anisomycin DB08437, Puromycin |
PTM databases
iPTMneti | P62917 |
MetOSitei | P62917 |
PhosphoSitePlusi | P62917 |
SwissPalmi | P62917 |
Polymorphism and mutation databases
BioMutai | RPL8 |
DMDMi | 51702823 |
Proteomic databases
EPDi | P62917 |
jPOSTi | P62917 |
MassIVEi | P62917 |
MaxQBi | P62917 |
PaxDbi | P62917 |
PeptideAtlasi | P62917 |
PRIDEi | P62917 |
ProteomicsDBi | 57454 |
TopDownProteomicsi | P62917 |
Protocols and materials databases
Antibodypediai | 14991, 284 antibodies |
DNASUi | 6132 |
Genome annotation databases
Ensembli | ENST00000262584; ENSP00000262584; ENSG00000161016 ENST00000394920; ENSP00000378378; ENSG00000161016 ENST00000528957; ENSP00000433464; ENSG00000161016 |
GeneIDi | 6132 |
KEGGi | hsa:6132 |
UCSCi | uc003zeb.4, human |
Organism-specific databases
CTDi | 6132 |
DisGeNETi | 6132 |
EuPathDBi | HostDB:ENSG00000161016.15 |
GeneCardsi | RPL8 |
HGNCi | HGNC:10368, RPL8 |
HPAi | ENSG00000161016, Low tissue specificity |
MIMi | 604177, gene |
neXtProti | NX_P62917 |
OpenTargetsi | ENSG00000161016 |
PharmGKBi | PA34768 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2309, Eukaryota |
GeneTreei | ENSGT00940000153244 |
InParanoidi | P62917 |
OMAi | SCIELRP |
OrthoDBi | 1156335at2759 |
PhylomeDBi | P62917 |
TreeFami | TF300748 |
Enzyme and pathway databases
PathwayCommonsi | P62917 |
Reactomei | R-HSA-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-HSA-156902, Peptide chain elongation R-HSA-1799339, SRP-dependent cotranslational protein targeting to membrane R-HSA-192823, Viral mRNA Translation R-HSA-2408557, Selenocysteine synthesis R-HSA-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-HSA-72689, Formation of a pool of free 40S subunits R-HSA-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-HSA-72764, Eukaryotic Translation Termination R-HSA-9010553, Regulation of expression of SLITs and ROBOs R-HSA-9633012, Response of EIF2AK4 (GCN2) to amino acid deficiency R-HSA-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-HSA-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
BioGRID-ORCSi | 6132, 780 hits in 847 CRISPR screens |
ChiTaRSi | RPL8, human |
GeneWikii | RPL8 |
GenomeRNAii | 6132 |
Pharosi | P62917, Tbio |
PROi | PR:P62917 |
RNActi | P62917, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000161016, Expressed in muscle tissue and 134 other tissues |
ExpressionAtlasi | P62917, baseline and differential |
Genevisiblei | P62917, HS |
Family and domain databases
Gene3Di | 2.30.30.30, 1 hit 4.10.950.10, 1 hit |
HAMAPi | MF_01320_A, Ribosomal_L2_A, 1 hit |
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR022666, Rbsml_prot_L2_RNA-bd_dom IPR014722, Rib_L2_dom2 IPR002171, Ribosomal_L2 IPR023672, Ribosomal_L2_arc IPR022669, Ribosomal_L2_C IPR022671, Ribosomal_L2_CS IPR014726, Ribosomal_L2_dom3 IPR008991, Translation_prot_SH3-like_sf |
PANTHERi | PTHR13691, PTHR13691, 1 hit |
Pfami | View protein in Pfam PF00181, Ribosomal_L2, 1 hit PF03947, Ribosomal_L2_C, 1 hit |
PIRSFi | PIRSF002158, Ribosomal_L2, 1 hit |
SMARTi | View protein in SMART SM01383, Ribosomal_L2, 1 hit SM01382, Ribosomal_L2_C, 1 hit |
SUPFAMi | SSF50104, SSF50104, 1 hit SSF50249, SSF50249, 1 hit |
PROSITEi | View protein in PROSITE PS00467, RIBOSOMAL_L2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RL8_HUMAN | |
Accessioni | P62917Primary (citable) accession number: P62917 Secondary accession number(s): A8K094 Q9BWQ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Ribosomal proteins
Ribosomal proteins families and list of entries