UniProtKB - P62916 (TF2B_RAT)
Transcription initiation factor IIB
Gtf2b
Functioni
Catalytic activityi
- EC:2.3.1.48By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 15 | ZincBy similarity | 1 | |
Metal bindingi | 18 | Zinc; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 34 | ZincBy similarity | 1 | |
Metal bindingi | 37 | ZincBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 11 – 42 | TFIIB-typePROSITE-ProRule annotationAdd BLAST | 32 |
GO - Molecular functioni
- acetyltransferase activity Source: UniProtKB
- core promoter sequence-specific DNA binding Source: RGD
- DNA binding Source: RGD
- histone acetyltransferase activity Source: UniProtKB-EC
- promoter-specific chromatin binding Source: UniProtKB
- RNA polymerase II complex binding Source: UniProtKB
- RNA polymerase II complex recruiting activity Source: GO_Central
- RNA polymerase II core promoter sequence-specific DNA binding Source: UniProtKB
- RNA polymerase II general transcription initiation factor activity Source: RGD
- TBP-class protein binding Source: RGD
- thyroid hormone receptor binding Source: RGD
- transcription factor binding Source: RGD
- zinc ion binding Source: UniProtKB
GO - Biological processi
- DNA-templated transcription, initiation Source: GO_Central
- positive regulation by host of viral transcription Source: RGD
- positive regulation of core promoter binding Source: RGD
- positive regulation of transcription initiation from RNA polymerase II promoter Source: RGD
- positive regulation of viral transcription Source: RGD
- protein acetylation Source: UniProtKB
- RNA polymerase II core complex assembly Source: UniProtKB
- RNA polymerase II preinitiation complex assembly Source: UniProtKB
- transcriptional start site selection at RNA polymerase II promoter Source: UniProtKB
- transcription by RNA polymerase II Source: UniProtKB
- transcription initiation from RNA polymerase II promoter Source: UniProtKB
- viral transcription Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, DNA-binding, Transferase |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-674695, RNA Polymerase II Pre-transcription Events R-RNO-6807505, RNA polymerase II transcribes snRNA genes R-RNO-73776, RNA Polymerase II Promoter Escape R-RNO-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-RNO-75953, RNA Polymerase II Transcription Initiation R-RNO-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription initiation factor IIBBy similarity (EC:2.3.1.48By similarity)Alternative name(s): General transcription factor TFIIBBy similarity RNA polymerase II alpha initiation factor |
Gene namesi | Name:Gtf2bImported |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619735, Gtf2b |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Chromosome By similarity
Note: Non-acetylated form colocalizes with DNA in the G0/1, S and G2 phases of the cell cycle, but not during mitosis. Acetylated form colocalizes at transcriptionally silent mitotic chromatids during mitosis at metaphase, anaphase, and telophase phases of the cell cycle.By similarity
Nucleus
- nucleus Source: UniProtKB
- transcription factor TFIID complex Source: RGD
Other locations
- chromosome Source: UniProtKB
- protein-DNA complex Source: UniProtKB
- RNA polymerase II transcription regulator complex Source: RGD
- transcription preinitiation complex Source: RGD
Keywords - Cellular componenti
Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119296 | 1 – 316 | Transcription initiation factor IIBAdd BLAST | 316 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 70 | PhosphoserineBy similarity | 1 | |
Modified residuei | 76 | PhosphoserineBy similarity | 1 | |
Modified residuei | 92 | PhosphoserineBy similarity | 1 | |
Modified residuei | 238 | N6-acetyllysine; by autocatalysisBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P62916 |
PaxDbi | P62916 |
PRIDEi | P62916 |
PTM databases
PhosphoSitePlusi | P62916 |
Interactioni
Subunit structurei
Found in a ternary complex with TATA box-bound TBP.
Part of a TFIID-containing RNA polymerase II pre-initiation complex (PIC) that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Associates with TFIID-TFIIA (DA complex) to form TFIID-TFIIA-TFIIB (DAB complex), which is then recognized by RNA polymerase II (Pol II).
Found in a RNA polymerase II initiation complex.
Interacts (via C-terminus) with TBP; this interaction with TATA box-bound TBP guides Pol II into the PIC.
Interacts (via N-terminus) with Pol II.
Interacts (via C-terminus) with SSU72; this interaction is inhibited by SYMPK.
Interacts with NR2F1; this interaction is direct.
Interacts with PGR.
Interacts with ESR1.
Interacts with GTF2F1 (via C-terminus and preferentially via acetylated form); this interaction prevents binding of GTF2B to GTF2F2.
Interacts with GTF2F2 (via N-terminus); this interaction is inhibited in presence of GTF2F1.
Interacts with the transcription elongation factor TCEA2.
Interacts with HSF1 (via transactivation domain) (By similarity).
Interacts with GPBP1 (By similarity).
By similarityGO - Molecular functioni
- RNA polymerase II complex binding Source: UniProtKB
- RNA polymerase II complex recruiting activity Source: GO_Central
- TBP-class protein binding Source: RGD
- thyroid hormone receptor binding Source: RGD
- transcription factor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 249570, 3 interactors |
IntActi | P62916, 2 interactors |
STRINGi | 10116.ENSRNOP00000015033 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 124 – 200 | 1Add BLAST | 77 | |
Repeati | 218 – 294 | 2Add BLAST | 77 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 189 – 193 | Core promoter DNA-bindingBy similarity | 5 | |
Regioni | 244 – 316 | Necessary for TATA box-bound TBP complex formationBy similarityAdd BLAST | 73 | |
Regioni | 249 – 252 | Core promoter DNA-bindingBy similarity | 4 | |
Regioni | 283 – 286 | Core promoter DNA-bindingBy similarity | 4 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 11 – 42 | TFIIB-typePROSITE-ProRule annotationAdd BLAST | 32 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1597, Eukaryota |
InParanoidi | P62916 |
OrthoDBi | 729732at2759 |
PhylomeDBi | P62916 |
TreeFami | TF105953 |
Family and domain databases
CDDi | cd00043, CYCLIN, 2 hits |
InterProi | View protein in InterPro IPR013763, Cyclin-like IPR036915, Cyclin-like_sf IPR000812, TFIIB IPR023486, TFIIB_CS IPR013150, TFIIB_cyclin IPR013137, Znf_TFIIB |
PANTHERi | PTHR11618, PTHR11618, 1 hit |
Pfami | View protein in Pfam PF08271, TF_Zn_Ribbon, 1 hit PF00382, TFIIB, 2 hits |
PRINTSi | PR00685, TIFACTORIIB |
SMARTi | View protein in SMART SM00385, CYCLIN, 2 hits |
SUPFAMi | SSF47954, SSF47954, 2 hits |
PROSITEi | View protein in PROSITE PS00782, TFIIB, 2 hits PS51134, ZF_TFIIB, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS
60 70 80 90 100
EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK
110 120 130 140 150
YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS
160 170 180 190 200
LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK
210 220 230 240 250
ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP
260 270 280 290 300
ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF
310
PSDFKFDTPV DKLPQL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JWZ8 | A0A0G2JWZ8_RAT | General transcription factor TFIIB | Gtf2b | 310 | Annotation score: | ||
A0A0A0MXW7 | A0A0A0MXW7_RAT | General transcription factor TFIIB | Gtf2b | 281 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65948 mRNA Translation: CAA46766.1 BC085345 mRNA Translation: AAH85345.1 |
PIRi | S26299 |
RefSeqi | NP_112303.1, NM_031041.1 |
Genome annotation databases
GeneIDi | 81673 |
KEGGi | rno:81673 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65948 mRNA Translation: CAA46766.1 BC085345 mRNA Translation: AAH85345.1 |
PIRi | S26299 |
RefSeqi | NP_112303.1, NM_031041.1 |
3D structure databases
SMRi | P62916 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 249570, 3 interactors |
IntActi | P62916, 2 interactors |
STRINGi | 10116.ENSRNOP00000015033 |
PTM databases
PhosphoSitePlusi | P62916 |
Proteomic databases
jPOSTi | P62916 |
PaxDbi | P62916 |
PRIDEi | P62916 |
Genome annotation databases
GeneIDi | 81673 |
KEGGi | rno:81673 |
Organism-specific databases
CTDi | 2959 |
RGDi | 619735, Gtf2b |
Phylogenomic databases
eggNOGi | KOG1597, Eukaryota |
InParanoidi | P62916 |
OrthoDBi | 729732at2759 |
PhylomeDBi | P62916 |
TreeFami | TF105953 |
Enzyme and pathway databases
Reactomei | R-RNO-674695, RNA Polymerase II Pre-transcription Events R-RNO-6807505, RNA polymerase II transcribes snRNA genes R-RNO-73776, RNA Polymerase II Promoter Escape R-RNO-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-RNO-75953, RNA Polymerase II Transcription Initiation R-RNO-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance |
Miscellaneous databases
PROi | PR:P62916 |
Family and domain databases
CDDi | cd00043, CYCLIN, 2 hits |
InterProi | View protein in InterPro IPR013763, Cyclin-like IPR036915, Cyclin-like_sf IPR000812, TFIIB IPR023486, TFIIB_CS IPR013150, TFIIB_cyclin IPR013137, Znf_TFIIB |
PANTHERi | PTHR11618, PTHR11618, 1 hit |
Pfami | View protein in Pfam PF08271, TF_Zn_Ribbon, 1 hit PF00382, TFIIB, 2 hits |
PRINTSi | PR00685, TIFACTORIIB |
SMARTi | View protein in SMART SM00385, CYCLIN, 2 hits |
SUPFAMi | SSF47954, SSF47954, 2 hits |
PROSITEi | View protein in PROSITE PS00782, TFIIB, 2 hits PS51134, ZF_TFIIB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TF2B_RAT | |
Accessioni | P62916Primary (citable) accession number: P62916 Secondary accession number(s): P29053 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | August 31, 2004 | |
Last modified: | February 10, 2021 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families