UniProtKB - P62908 (RS3_MOUSE)
40S ribosomal protein S3
Rps3
Functioni
Catalytic activityi
- The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.1 Publication EC:4.2.99.18
GO - Molecular functioni
- class I DNA-(apurinic or apyrimidinic site) endonuclease activity Source: UniProtKB-EC
- damaged DNA binding Source: MGI
- DNA-(apurinic or apyrimidinic site) endonuclease activity Source: UniProtKB
- DNA binding Source: MGI
- endodeoxyribonuclease activity Source: MGI
- enzyme binding Source: MGI
- Hsp70 protein binding Source: MGI
- Hsp90 protein binding Source: MGI
- kinase binding Source: MGI
- microtubule binding Source: MGI
- mRNA binding Source: MGI
- oxidized purine DNA binding Source: MGI
- oxidized pyrimidine DNA binding Source: MGI
- protein-containing complex binding Source: MGI
- protein kinase A binding Source: MGI
- protein kinase binding Source: MGI
- RNA binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: Ensembl
- small ribosomal subunit rRNA binding Source: MGI
- structural constituent of ribosome Source: GO_Central
- supercoiled DNA binding Source: MGI
- transcription factor binding Source: MGI
- tubulin binding Source: MGI
- ubiquitin-like protein conjugating enzyme binding Source: MGI
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cellular response to DNA damage stimulus Source: MGI
- cellular response to hydrogen peroxide Source: MGI
- cellular response to nerve growth factor stimulus Source: MGI
- cellular response to reactive oxygen species Source: MGI
- cellular response to tumor necrosis factor Source: MGI
- chromosome segregation Source: MGI
- DNA damage response, detection of DNA damage Source: MGI
- DNA repair Source: UniProtKB-KW
- negative regulation of DNA repair Source: MGI
- negative regulation of protein ubiquitination Source: MGI
- negative regulation of translation Source: MGI
- positive regulation of activated T cell proliferation Source: MGI
- positive regulation of apoptotic signaling pathway Source: MGI
- positive regulation of base-excision repair Source: MGI
- positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis Source: MGI
- positive regulation of DNA N-glycosylase activity Source: MGI
- positive regulation of DNA repair Source: MGI
- positive regulation of endodeoxyribonuclease activity Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of interleukin-2 production Source: MGI
- positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
- positive regulation of JUN kinase activity Source: MGI
- positive regulation of microtubule polymerization Source: MGI
- positive regulation of NF-kappaB transcription factor activity Source: CAFA
- positive regulation of NIK/NF-kappaB signaling Source: MGI
- positive regulation of protein-containing complex assembly Source: CAFA
- positive regulation of T cell receptor signaling pathway Source: MGI
- regulation of apoptotic process Source: MGI
- response to TNF agonist Source: MGI
- spindle assembly Source: MGI
- translation Source: InterPro
Keywordsi
Molecular function | DNA-binding, Lyase, Ribonucleoprotein, Ribosomal protein, RNA-binding |
Biological process | Apoptosis, Cell cycle, Cell division, DNA damage, DNA repair, Mitosis, Transcription, Transcription regulation, Translation regulation |
Enzyme and pathway databases
Reactomei | R-MMU-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-MMU-1799339, SRP-dependent cotranslational protein targeting to membrane R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-MMU-72649, Translation initiation complex formation R-MMU-72689, Formation of a pool of free 40S subunits R-MMU-72695, Formation of the ternary complex, and subsequently, the 43S complex R-MMU-72702, Ribosomal scanning and start codon recognition R-MMU-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-MMU-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Rps3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1350917, Rps3 |
Subcellular locationi
Cytoskeleton
- spindle By similarity
Mitochondrion
- Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity
Nucleus
Other locations
- Cytoplasm 1 Publication
Note: In normal cells, located mainly in the cytoplasm with small amounts in the nucleus but translocates to the nucleus in cells undergoing apoptosis. Nuclear translocation is also induced by DNA damaging agents such as hydrogen peroxide (By similarity). Accumulates in the mitochondrion in response to increased ROS levels (By similarity). Localizes to the spindle during mitosis (By similarity). Localized in cytoplasmic mRNP granules containing untranslated mRNAs (By similarity).By similarity1 Publication
Cytoskeleton
- mitotic spindle Source: MGI
Cytosol
- cytosol Source: MGI
- cytosolic small ribosomal subunit Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Mitochondrion
- mitochondrial inner membrane Source: MGI
- mitochondrial matrix Source: MGI
Nucleus
Plasma Membrane
- plasma membrane Source: MGI
- ruffle membrane Source: MGI
Other locations
- cytoplasm Source: MGI
- dendrite Source: MGI
- endomembrane system Source: MGI
- NF-kappaB complex Source: MGI
- polysome Source: MGI
- postsynaptic density Source: MGI
- ribonucleoprotein complex Source: UniProtKB
- ribosome Source: MGI
- synapse Source: SynGO
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Membrane, Mitochondrion, Mitochondrion inner membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000130321 | 2 – 243 | 40S ribosomal protein S3Add BLAST | 242 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 6 | Phosphoserine; by PKC/PRKCDBy similarity | 1 | |
Modified residuei | 35 | PhosphoserineBy similarity | 1 | |
Modified residuei | 42 | Phosphothreonine; by MAPKBy similarity | 1 | |
Modified residuei | 62 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 64 | Asymmetric dimethylarginine; by PRMT1By similarity | 1 | |
Modified residuei | 65 | Asymmetric dimethylarginine; by PRMT1By similarity | 1 | |
Modified residuei | 67 | Asymmetric dimethylarginine; by PRMT1By similarity | 1 | |
Modified residuei | 70 | Phosphothreonine; by PKBBy similarity | 1 | |
Cross-linki | 90 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 104 | PhosphoserineBy similarity | 1 | |
Modified residuei | 132 | N6-succinyllysineCombined sources | 1 | |
Cross-linki | 202 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 209 | Phosphoserine; by IKKBBy similarity | 1 | |
Cross-linki | 214 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 214 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Modified residuei | 220 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 221 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 224 | PhosphoserineBy similarity | 1 | |
Cross-linki | 230 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 242 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
CPTACi | non-CPTAC-3873 |
EPDi | P62908 |
jPOSTi | P62908 |
PaxDbi | P62908 |
PeptideAtlasi | P62908 |
PRIDEi | P62908 |
PTM databases
iPTMneti | P62908 |
PhosphoSitePlusi | P62908 |
SwissPalmi | P62908 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000030744, Expressed in dorsal pancreas and 274 other tissues |
Genevisiblei | P62908, MM |
Interactioni
Subunit structurei
Component of the 40S small ribosomal subunit.
Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs.
Interacts with HNRPD.
Interacts with PRMT1; the interaction methylates RPS3.
Interacts with SUMO1; the interaction sumoylates RPS3.
Interacts with UBC9.
Interacts with CDK1; the interaction phosphorylates RPS3.
Interacts with PRKCD; the interaction phosphorylates RPS3.
Interacts with PKB/AKT; the interaction phosphorylates RPS3.
Interacts with E2F1; the interaction occurs in the absence of nerve growth factor and increases transcription of pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5.
Interacts with the base excision repair proteins APEX1 and OGG1; interaction with OGG1 increases OGG1 N-glycosylase activity.
Interacts with UNG; the interaction increases the uracil excision activity of UNG1.
Interacts with HSP90; the interaction prevents the ubiquitination and proteasome-dependent degradation of RPS3 and is suppressed by increased ROS levels.
Interacts with TOM70; the interaction promotes translocation of RPS3 to the mitochondrion.
Interacts (via N-terminus) with RELA (via N-terminus); the interaction enhances the DNA-binding activity of the NF-kappa-B p65-p50 complex.
Interacts with NFKBIA; the interaction is direct and may bridge the interaction between RPS3 and RELA.
Interacts with IKKB; the interaction phosphorylates RPS3 and enhances its translocation to the nucleus.
Interacts (via KH domain) with MDM2 and TP53.
Interacts with TRADD.
Interacts with CRY1.
By similarity1 PublicationGO - Molecular functioni
- enzyme binding Source: MGI
- Hsp70 protein binding Source: MGI
- Hsp90 protein binding Source: MGI
- kinase binding Source: MGI
- microtubule binding Source: MGI
- protein kinase A binding Source: MGI
- protein kinase binding Source: MGI
- transcription factor binding Source: MGI
- tubulin binding Source: MGI
- ubiquitin-like protein conjugating enzyme binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 205106, 119 interactors |
ComplexPortali | CPX-5261, 40S cytosolic small ribosomal subunit |
CORUMi | P62908 |
IntActi | P62908, 15 interactors |
MINTi | P62908 |
STRINGi | 10090.ENSMUSP00000032998 |
Miscellaneous databases
RNActi | P62908, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 92 | KH type-2PROSITE-ProRule annotationAdd BLAST | 72 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3181, Eukaryota |
GeneTreei | ENSGT00390000008610 |
HOGENOMi | CLU_058591_2_1_1 |
InParanoidi | P62908 |
OMAi | MLPHDPE |
OrthoDBi | 1135751at2759 |
PhylomeDBi | P62908 |
TreeFami | TF300901 |
Family and domain databases
Gene3Di | 3.30.1140.32, 1 hit 3.30.300.20, 1 hit |
InterProi | View protein in InterPro IPR015946, KH_dom-like_a/b IPR004044, KH_dom_type_2 IPR009019, KH_sf_prok-type IPR001351, Ribosomal_S3_C IPR036419, Ribosomal_S3_C_sf IPR018280, Ribosomal_S3_CS IPR005703, Ribosomal_S3_euk/arc |
Pfami | View protein in Pfam PF07650, KH_2, 1 hit PF00189, Ribosomal_S3_C, 1 hit |
SUPFAMi | SSF54814, SSF54814, 1 hit SSF54821, SSF54821, 1 hit |
TIGRFAMsi | TIGR01008, uS3_euk_arch, 1 hit |
PROSITEi | View protein in PROSITE PS50823, KH_TYPE_2, 1 hit PS00548, RIBOSOMAL_S3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII
60 70 80 90 100
LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAEK VATRGLCAIA
110 120 130 140 150
QAESLRYKLL GGLAVRRACY GVLRFIMESG AKGCEVVVSG KLRGQRAKSM
160 170 180 190 200
KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPSGKIGP
210 220 230 240
KKPLPDHVSI VEPKDEILPT TPISEQKGGK PEPPAMPQPV PTA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YV43 | D3YV43_MOUSE | 40S ribosomal protein S3 | Rps3 | 191 | Annotation score: | ||
A0A140LI77 | A0A140LI77_MOUSE | 40S ribosomal protein S3 | Rps3 | 144 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76772 mRNA Translation: CAA54167.1 BC010721 mRNA Translation: AAH10721.1 |
CCDSi | CCDS40031.1 |
PIRi | S41170 |
RefSeqi | NP_036182.1, NM_012052.2 |
Genome annotation databases
Ensembli | ENSMUST00000032998; ENSMUSP00000032998; ENSMUSG00000030744 |
GeneIDi | 27050 |
KEGGi | mmu:27050 |
UCSCi | uc009ilr.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76772 mRNA Translation: CAA54167.1 BC010721 mRNA Translation: AAH10721.1 |
CCDSi | CCDS40031.1 |
PIRi | S41170 |
RefSeqi | NP_036182.1, NM_012052.2 |
3D structure databases
BMRBi | P62908 |
SMRi | P62908 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 205106, 119 interactors |
ComplexPortali | CPX-5261, 40S cytosolic small ribosomal subunit |
CORUMi | P62908 |
IntActi | P62908, 15 interactors |
MINTi | P62908 |
STRINGi | 10090.ENSMUSP00000032998 |
PTM databases
iPTMneti | P62908 |
PhosphoSitePlusi | P62908 |
SwissPalmi | P62908 |
Proteomic databases
CPTACi | non-CPTAC-3873 |
EPDi | P62908 |
jPOSTi | P62908 |
PaxDbi | P62908 |
PeptideAtlasi | P62908 |
PRIDEi | P62908 |
Genome annotation databases
Ensembli | ENSMUST00000032998; ENSMUSP00000032998; ENSMUSG00000030744 |
GeneIDi | 27050 |
KEGGi | mmu:27050 |
UCSCi | uc009ilr.2, mouse |
Organism-specific databases
CTDi | 6188 |
MGIi | MGI:1350917, Rps3 |
Phylogenomic databases
eggNOGi | KOG3181, Eukaryota |
GeneTreei | ENSGT00390000008610 |
HOGENOMi | CLU_058591_2_1_1 |
InParanoidi | P62908 |
OMAi | MLPHDPE |
OrthoDBi | 1135751at2759 |
PhylomeDBi | P62908 |
TreeFami | TF300901 |
Enzyme and pathway databases
Reactomei | R-MMU-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-MMU-1799339, SRP-dependent cotranslational protein targeting to membrane R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-MMU-72649, Translation initiation complex formation R-MMU-72689, Formation of a pool of free 40S subunits R-MMU-72695, Formation of the ternary complex, and subsequently, the 43S complex R-MMU-72702, Ribosomal scanning and start codon recognition R-MMU-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-MMU-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
BioGRID-ORCSi | 27050, 8 hits in 17 CRISPR screens |
ChiTaRSi | Rps3, mouse |
PROi | PR:P62908 |
RNActi | P62908, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030744, Expressed in dorsal pancreas and 274 other tissues |
Genevisiblei | P62908, MM |
Family and domain databases
Gene3Di | 3.30.1140.32, 1 hit 3.30.300.20, 1 hit |
InterProi | View protein in InterPro IPR015946, KH_dom-like_a/b IPR004044, KH_dom_type_2 IPR009019, KH_sf_prok-type IPR001351, Ribosomal_S3_C IPR036419, Ribosomal_S3_C_sf IPR018280, Ribosomal_S3_CS IPR005703, Ribosomal_S3_euk/arc |
Pfami | View protein in Pfam PF07650, KH_2, 1 hit PF00189, Ribosomal_S3_C, 1 hit |
SUPFAMi | SSF54814, SSF54814, 1 hit SSF54821, SSF54821, 1 hit |
TIGRFAMsi | TIGR01008, uS3_euk_arch, 1 hit |
PROSITEi | View protein in PROSITE PS50823, KH_TYPE_2, 1 hit PS00548, RIBOSOMAL_S3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RS3_MOUSE | |
Accessioni | P62908Primary (citable) accession number: P62908 Secondary accession number(s): P17073, P47933 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | August 31, 2004 | |
Last modified: | December 2, 2020 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Ribosomal proteins
Ribosomal proteins families and list of entries