UniProtKB - P62882 (GNB5_RAT)
Protein
Guanine nucleotide-binding protein subunit beta-5
Gene
Gnb5
Organism
Rattus norvegicus (Rat)
Status
Functioni
Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation (Probable). Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D2 dopamine receptors (By similarity). May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate (By similarity).By similarityCurated
GO - Molecular functioni
- chaperone binding Source: UniProtKB
- G-protein gamma-subunit binding Source: UniProtKB
- GTPase activating protein binding Source: RGD
- GTPase activator activity Source: RGD
GO - Biological processi
- dopamine receptor signaling pathway Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: RGD
- negative regulation of voltage-gated calcium channel activity Source: UniProtKB
- positive regulation of GTPase activity Source: RGD
- signal transduction Source: RGD
Keywordsi
Molecular function | Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-1296041, Activation of G protein gated Potassium channels R-RNO-202040, G-protein activation R-RNO-2514859, Inactivation, recovery and regulation of the phototransduction cascade R-RNO-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-RNO-392170, ADP signalling through P2Y purinoceptor 12 R-RNO-392451, G beta:gamma signalling through PI3Kgamma R-RNO-400042, Adrenaline,noradrenaline inhibits insulin secretion R-RNO-4086398, Ca2+ pathway R-RNO-416476, G alpha (q) signalling events R-RNO-418594, G alpha (i) signalling events R-RNO-418597, G alpha (z) signalling events R-RNO-420092, Glucagon-type ligand receptors R-RNO-428930, Thromboxane signalling through TP receptor R-RNO-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-RNO-456926, Thrombin signalling through proteinase activated receptors (PARs) R-RNO-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-RNO-8964616, G beta:gamma signalling through CDC42 R-RNO-997272, Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide-binding protein subunit beta-5Alternative name(s): Gbeta5 Transducin beta chain 5 |
Gene namesi | Name:Gnb5 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620759, Gnb5 |
Subcellular locationi
Other locations
- Membrane By similarity
Cytosol
- cytosol Source: RGD
Nucleus
- nucleus Source: RGD
Plasma Membrane
- heterotrimeric G-protein complex Source: GO_Central
- plasma membrane Source: RGD
Other locations
- cell body Source: RGD
- cytoplasm Source: GO_Central
- photoreceptor inner segment Source: RGD
- photoreceptor outer segment Source: RGD
- presynapse Source: RGD
- protein-containing complex Source: RGD
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127709 | 1 – 353 | Guanine nucleotide-binding protein subunit beta-5Add BLAST | 353 |
Proteomic databases
jPOSTi | P62882 |
PaxDbi | P62882 |
PRIDEi | P62882 |
PTM databases
PhosphoSitePlusi | P62882 |
Expressioni
Tissue specificityi
Detected in brain.1 Publication
Interactioni
Subunit structurei
Component of a complex composed of RGS9, GNB5 and RGS9BP; within this complex, the presence of GNB5 stabilizes both itself and RGS9 and increases RGS9 GTPase-activating protein (GAP) activity (By similarity).
Interacts with RGS6 and RGS7 (By similarity).
By similarityGO - Molecular functioni
- chaperone binding Source: UniProtKB
- G-protein gamma-subunit binding Source: UniProtKB
- GTPase activating protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 249765, 1 interactor |
CORUMi | P62882 |
IntActi | P62882, 2 interactors |
MINTi | P62882 |
STRINGi | 10116.ENSRNOP00000066690 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 61 – 100 | WD 1Add BLAST | 40 | |
Repeati | 103 – 142 | WD 2Add BLAST | 40 | |
Repeati | 151 – 192 | WD 3Add BLAST | 42 | |
Repeati | 194 – 236 | WD 4Add BLAST | 43 | |
Repeati | 237 – 276 | WD 5Add BLAST | 40 | |
Repeati | 278 – 320 | WD 6Add BLAST | 43 | |
Repeati | 323 – 352 | WD 7Add BLAST | 30 |
Sequence similaritiesi
Belongs to the WD repeat G protein beta family.Curated
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0286, Eukaryota |
InParanoidi | P62882 |
OrthoDBi | 704786at2759 |
PhylomeDBi | P62882 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR001632, Gprotein_B IPR016346, Guanine_nucleotide-bd_bsu IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR19850, PTHR19850, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 5 hits |
PRINTSi | PR00319, GPROTEINB PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 3 hits PS50082, WD_REPEATS_2, 6 hits PS50294, WD_REPEATS_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P62882-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATDGLHENE TLASLKSEAE SLKGKLEEER AKLHDVELHQ VAERVEALGQ
60 70 80 90 100
FVMKTRRTLK GHGNKVLCMD WCKDKRRIVS SSQDGKVIVW DSFTTNKEHA
110 120 130 140 150
VTMPCTWVMA CAYAPSGCAI ACGGLDNKCS VYPLTFDKNE NMAAKKKSVA
160 170 180 190 200
MHTNYLSACS FTNSDMQILT ASGDGTCALW DVESGQLLQS FHGHGADVLC
210 220 230 240 250
LDLAPSETGN TFVSGGCDKK AMVWDMRSGQ CVQAFETHES DVNSVRYYPS
260 270 280 290 300
GDAFASGSDD ATCRLYDLRA DREVAIYSKE SIIFGASSVD FSLSGRLLFA
310 320 330 340 350
GYNDYTINVW DVLKGSRVSI LFGHENRVST LRVSPDGTAF CSGSWDHTLR
VWA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3ZZ54 | D3ZZ54_RAT | Guanine nucleotide-binding protein ... | Gnb5 | 352 | Annotation score: | ||
M0RAX4 | M0RAX4_RAT | Guanine nucleotide-binding protein ... | Gnb5 | 351 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF001953 mRNA Translation: AAB59974.1 AY552803 mRNA Translation: AAS59141.1 BC089221 mRNA Translation: AAH89221.1 AF022086 mRNA Translation: AAB82553.1 |
RefSeqi | NP_113958.1, NM_031770.3 |
Genome annotation databases
GeneIDi | 83579 |
KEGGi | rno:83579 |
UCSCi | RGD:620759, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF001953 mRNA Translation: AAB59974.1 AY552803 mRNA Translation: AAS59141.1 BC089221 mRNA Translation: AAH89221.1 AF022086 mRNA Translation: AAB82553.1 |
RefSeqi | NP_113958.1, NM_031770.3 |
3D structure databases
SMRi | P62882 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 249765, 1 interactor |
CORUMi | P62882 |
IntActi | P62882, 2 interactors |
MINTi | P62882 |
STRINGi | 10116.ENSRNOP00000066690 |
PTM databases
PhosphoSitePlusi | P62882 |
Proteomic databases
jPOSTi | P62882 |
PaxDbi | P62882 |
PRIDEi | P62882 |
Genome annotation databases
GeneIDi | 83579 |
KEGGi | rno:83579 |
UCSCi | RGD:620759, rat |
Organism-specific databases
CTDi | 10681 |
RGDi | 620759, Gnb5 |
Phylogenomic databases
eggNOGi | KOG0286, Eukaryota |
InParanoidi | P62882 |
OrthoDBi | 704786at2759 |
PhylomeDBi | P62882 |
Enzyme and pathway databases
Reactomei | R-RNO-1296041, Activation of G protein gated Potassium channels R-RNO-202040, G-protein activation R-RNO-2514859, Inactivation, recovery and regulation of the phototransduction cascade R-RNO-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-RNO-392170, ADP signalling through P2Y purinoceptor 12 R-RNO-392451, G beta:gamma signalling through PI3Kgamma R-RNO-400042, Adrenaline,noradrenaline inhibits insulin secretion R-RNO-4086398, Ca2+ pathway R-RNO-416476, G alpha (q) signalling events R-RNO-418594, G alpha (i) signalling events R-RNO-418597, G alpha (z) signalling events R-RNO-420092, Glucagon-type ligand receptors R-RNO-428930, Thromboxane signalling through TP receptor R-RNO-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-RNO-456926, Thrombin signalling through proteinase activated receptors (PARs) R-RNO-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-RNO-8964616, G beta:gamma signalling through CDC42 R-RNO-997272, Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits |
Miscellaneous databases
PROi | PR:P62882 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR001632, Gprotein_B IPR016346, Guanine_nucleotide-bd_bsu IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR19850, PTHR19850, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 5 hits |
PRINTSi | PR00319, GPROTEINB PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 3 hits PS50082, WD_REPEATS_2, 6 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNB5_RAT | |
Accessioni | P62882Primary (citable) accession number: P62882 Secondary accession number(s): O35354 Q91WB3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | August 16, 2004 | |
Last modified: | December 2, 2020 | |
This is version 108 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families