UniProtKB - P62834 (RAP1A_HUMAN)
Ras-related protein Rap-1A
RAP1A
Functioni
Catalytic activityi
- EC:3.6.5.2By similarity
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 60 | GTP; via amide nitrogen2 Publications | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 18 | GTP2 Publications | 9 | |
Nucleotide bindingi | 29 – 35 | GTP2 Publications | 7 | |
Nucleotide bindingi | 116 – 119 | GTP2 Publications | 4 |
GO - Molecular functioni
- GDP binding Source: GO_Central
- GTPase activity Source: GO_Central
- GTP binding Source: GO_Central
- guanyl-nucleotide exchange factor activity Source: UniProtKB
- protein-containing complex binding Source: MGI
- small GTPase binding Source: Ensembl
GO - Biological processi
- activation of MAPKK activity Source: Reactome
- adaptive immune response Source: Reactome
- cellular response to cAMP Source: UniProtKB
- cellular response to drug Source: Ensembl
- cellular response to nerve growth factor stimulus Source: UniProtKB
- establishment of endothelial barrier Source: UniProtKB
- liver regeneration Source: Ensembl
- negative regulation of collagen biosynthetic process Source: Ensembl
- negative regulation of synaptic vesicle exocytosis Source: GO_Central
- nerve growth factor signaling pathway Source: UniProtKB
- nervous system development Source: UniProtKB-KW
- neutrophil degranulation Source: Reactome
- positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
- positive regulation of Fc-gamma receptor signaling pathway involved in phagocytosis Source: Ensembl
- positive regulation of glucose import Source: Ensembl
- positive regulation of GTPase activity Source: UniProtKB
- positive regulation of neuron projection development Source: UniProtKB
- positive regulation of protein kinase activity Source: UniProtKB
- positive regulation of vasculogenesis Source: UniProtKB
- protein localization to plasma membrane Source: GO_Central
- Rap protein signal transduction Source: UniProtKB
- regulation of cell junction assembly Source: UniProtKB
- regulation of insulin secretion Source: Reactome
- regulation of neurotransmitter receptor localization to postsynaptic specialization membrane Source: SynGO
- response to antineoplastic agent Source: Ensembl
- response to carbohydrate Source: Ensembl
Keywordsi
Molecular function | Hydrolase |
Biological process | Neurogenesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P62834 |
Reactomei | R-HSA-170968, Frs2-mediated activation R-HSA-170984, ARMS-mediated activation R-HSA-354192, Integrin signaling R-HSA-354194, GRB2:SOS provides linkage to MAPK signaling for Integrins R-HSA-372708, p130Cas linkage to MAPK signaling for integrins R-HSA-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-HSA-392517, Rap1 signalling R-HSA-5674135, MAP2K and MAPK activation R-HSA-6798695, Neutrophil degranulation R-HSA-6802946, Signaling by moderate kinase activity BRAF mutants R-HSA-6802948, Signaling by high-kinase activity BRAF mutants R-HSA-6802952, Signaling by BRAF and RAF fusions R-HSA-6802955, Paradoxical activation of RAF signaling by kinase inactive BRAF R-HSA-8875555, MET activates RAP1 and RAC1 R-HSA-9649948, Signaling downstream of RAS mutants R-HSA-9656223, Signaling by RAF1 mutants |
SignaLinki | P62834 |
SIGNORi | P62834 |
Names & Taxonomyi
Protein namesi | Recommended name: Ras-related protein Rap-1A (EC:3.6.5.2By similarity)Alternative name(s): C21KG G-22K GTP-binding protein smg p21A Ras-related protein Krev-1 |
Gene namesi | Name:RAP1A Synonyms:KREV1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9855, RAP1A |
MIMi | 179520, gene |
neXtProti | NX_P62834 |
VEuPathDBi | HostDB:ENSG00000116473.14 |
Subcellular locationi
Endosome
- Early endosome By similarity
Plasma membrane
- Cell membrane 1 Publication; Lipid-anchor 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- perinuclear region 1 Publication
- Cell junction By similarity
Note: Recruited from early endosome to late endosome compartment after nerve growth factor (NGF) stimulation. Localized with RAPGEF2 at cell-cell junctions (By similarity). Colocalized with RAPGEF2 in the perinuclear region.By similarity
Cytosol
- cytosol Source: Reactome
Endosome
- early endosome Source: UniProtKB
- endosome membrane Source: Reactome
- late endosome Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- cell junction Source: UniProtKB
- cytoplasm Source: UniProtKB
- glutamatergic synapse Source: SynGO
- guanyl-nucleotide exchange factor complex Source: Ensembl
- neuron projection Source: Ensembl
- perinuclear region of cytoplasm Source: UniProtKB
- phagocytic vesicle Source: Ensembl
- specific granule membrane Source: Reactome
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Endosome, MembranePathology & Biotechi
Keywords - Diseasei
Tumor suppressorOrganism-specific databases
DisGeNETi | 5906 |
MalaCardsi | RAP1A |
OpenTargetsi | ENSG00000116473 |
PharmGKBi | PA34217 |
Miscellaneous databases
Pharosi | P62834, Tchem |
Chemistry databases
ChEMBLi | CHEMBL1255139 |
Genetic variation databases
BioMutai | RAP1A |
DMDMi | 51338607 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000030199 | 1 – 181 | Ras-related protein Rap-1AAdd BLAST | 181 | |
PropeptideiPRO_0000030200 | 182 – 184 | Removed in mature form | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 181 | Cysteine methyl ester1 Publication | 1 | |
Lipidationi | 181 | S-geranylgeranyl cysteine1 Publication | 1 |
Keywords - PTMi
Lipoprotein, Methylation, PrenylationProteomic databases
EPDi | P62834 |
jPOSTi | P62834 |
MassIVEi | P62834 |
MaxQBi | P62834 |
PaxDbi | P62834 |
PeptideAtlasi | P62834 |
PRIDEi | P62834 |
ProteomicsDBi | 57431 |
TopDownProteomicsi | P62834 |
2D gel databases
OGPi | P62834 |
PTM databases
iPTMneti | P62834 |
PhosphoSitePlusi | P62834 |
SwissPalmi | P62834 |
Expressioni
Gene expression databases
Bgeei | ENSG00000116473, Expressed in cauda epididymis and 249 other tissues |
ExpressionAtlasi | P62834, baseline and differential |
Genevisiblei | P62834, HS |
Organism-specific databases
HPAi | ENSG00000116473, Low tissue specificity |
Interactioni
Subunit structurei
Found in a complex, at least composed of ITGB1BP1, KRIT1 and RAP1A.
Interacts (active GTP-bound form preferentially) with KRIT1 (via C-terminus FERM domain); the interaction does not induce the opening conformation of KRIT1. In its GTP-bound form interacts with PLCE1 and RADIL.
Interacts with SGSM1, SGSM2 and SGSM3.
Interacts (via GTP-bound active form) with RAPGEF2 (via Ras-associating domain).
7 PublicationsBinary interactionsi
Hide detailsP62834
With | #Exp. | IntAct |
---|---|---|
RAF1 [P04049] | 2 | EBI-491414,EBI-365996 |
RASSF5 - isoform 2 [Q8WWW0-2] | 3 | EBI-491414,EBI-960502 |
Rassf5 [Q5EBH1] from Mus musculus. | 3 | EBI-491414,EBI-960530 |
GO - Molecular functioni
- small GTPase binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 111841, 116 interactors |
CORUMi | P62834 |
DIPi | DIP-29106N |
IntActi | P62834, 40 interactors |
MINTi | P62834 |
STRINGi | 9606.ENSP00000358723 |
Chemistry databases
BindingDBi | P62834 |
Miscellaneous databases
RNActi | P62834, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P62834 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P62834 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 32 – 40 | Effector regionCurated | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000160251 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P62834 |
OMAi | QREYHIA |
PhylomeDBi | P62834 |
TreeFami | TF313014 |
Family and domain databases
CDDi | cd04175, Rap1, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR038851, Rap1 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ
60 70 80 90 100
CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI
110 120 130 140 150
LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS
160 170 180
KINVNEIFYD LVRQINRKTP VEKKKPKKKS CLLL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A075B6Q0 | A0A075B6Q0_HUMAN | Ras-related protein Rap-1A | RAP1A | 129 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12533 mRNA Translation: CAA31051.1 M22995 mRNA Translation: AAA36150.1 AF493912 mRNA Translation: AAM12626.1 AL049557 Genomic DNA No translation available. BC014086 mRNA Translation: AAH14086.1 |
CCDSi | CCDS840.1 |
PIRi | A32342 |
RefSeqi | NP_001010935.1, NM_001010935.2 NP_001278825.1, NM_001291896.1 NP_002875.1, NM_002884.3 XP_016857451.1, XM_017001962.1 XP_016857452.1, XM_017001963.1 XP_016857453.1, XM_017001964.1 |
Genome annotation databases
Ensembli | ENST00000356415; ENSP00000348786; ENSG00000116473 ENST00000369709; ENSP00000358723; ENSG00000116473 |
GeneIDi | 5906 |
KEGGi | hsa:5906 |
UCSCi | uc001ebi.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12533 mRNA Translation: CAA31051.1 M22995 mRNA Translation: AAA36150.1 AF493912 mRNA Translation: AAM12626.1 AL049557 Genomic DNA No translation available. BC014086 mRNA Translation: AAH14086.1 |
CCDSi | CCDS840.1 |
PIRi | A32342 |
RefSeqi | NP_001010935.1, NM_001010935.2 NP_001278825.1, NM_001291896.1 NP_002875.1, NM_002884.3 XP_016857451.1, XM_017001962.1 XP_016857452.1, XM_017001963.1 XP_016857453.1, XM_017001964.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1C1Y | X-ray | 1.90 | A | 1-167 | [»] | |
1GUA | X-ray | 2.00 | A | 1-167 | [»] | |
3KUC | X-ray | 1.92 | A | 1-167 | [»] | |
4KVG | X-ray | 1.65 | A/C | 1-167 | [»] | |
SMRi | P62834 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111841, 116 interactors |
CORUMi | P62834 |
DIPi | DIP-29106N |
IntActi | P62834, 40 interactors |
MINTi | P62834 |
STRINGi | 9606.ENSP00000358723 |
Chemistry databases
BindingDBi | P62834 |
ChEMBLi | CHEMBL1255139 |
PTM databases
iPTMneti | P62834 |
PhosphoSitePlusi | P62834 |
SwissPalmi | P62834 |
Genetic variation databases
BioMutai | RAP1A |
DMDMi | 51338607 |
2D gel databases
OGPi | P62834 |
Proteomic databases
EPDi | P62834 |
jPOSTi | P62834 |
MassIVEi | P62834 |
MaxQBi | P62834 |
PaxDbi | P62834 |
PeptideAtlasi | P62834 |
PRIDEi | P62834 |
ProteomicsDBi | 57431 |
TopDownProteomicsi | P62834 |
Protocols and materials databases
Antibodypediai | 4330, 459 antibodies |
DNASUi | 5906 |
Genome annotation databases
Ensembli | ENST00000356415; ENSP00000348786; ENSG00000116473 ENST00000369709; ENSP00000358723; ENSG00000116473 |
GeneIDi | 5906 |
KEGGi | hsa:5906 |
UCSCi | uc001ebi.4, human |
Organism-specific databases
CTDi | 5906 |
DisGeNETi | 5906 |
GeneCardsi | RAP1A |
HGNCi | HGNC:9855, RAP1A |
HPAi | ENSG00000116473, Low tissue specificity |
MalaCardsi | RAP1A |
MIMi | 179520, gene |
neXtProti | NX_P62834 |
OpenTargetsi | ENSG00000116473 |
PharmGKBi | PA34217 |
VEuPathDBi | HostDB:ENSG00000116473.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000160251 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P62834 |
OMAi | QREYHIA |
PhylomeDBi | P62834 |
TreeFami | TF313014 |
Enzyme and pathway databases
PathwayCommonsi | P62834 |
Reactomei | R-HSA-170968, Frs2-mediated activation R-HSA-170984, ARMS-mediated activation R-HSA-354192, Integrin signaling R-HSA-354194, GRB2:SOS provides linkage to MAPK signaling for Integrins R-HSA-372708, p130Cas linkage to MAPK signaling for integrins R-HSA-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-HSA-392517, Rap1 signalling R-HSA-5674135, MAP2K and MAPK activation R-HSA-6798695, Neutrophil degranulation R-HSA-6802946, Signaling by moderate kinase activity BRAF mutants R-HSA-6802948, Signaling by high-kinase activity BRAF mutants R-HSA-6802952, Signaling by BRAF and RAF fusions R-HSA-6802955, Paradoxical activation of RAF signaling by kinase inactive BRAF R-HSA-8875555, MET activates RAP1 and RAC1 R-HSA-9649948, Signaling downstream of RAS mutants R-HSA-9656223, Signaling by RAF1 mutants |
SignaLinki | P62834 |
SIGNORi | P62834 |
Miscellaneous databases
BioGRID-ORCSi | 5906, 27 hits in 963 CRISPR screens |
ChiTaRSi | RAP1A, human |
EvolutionaryTracei | P62834 |
GeneWikii | RAP1A |
GenomeRNAii | 5906 |
Pharosi | P62834, Tchem |
PROi | PR:P62834 |
RNActi | P62834, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000116473, Expressed in cauda epididymis and 249 other tissues |
ExpressionAtlasi | P62834, baseline and differential |
Genevisiblei | P62834, HS |
Family and domain databases
CDDi | cd04175, Rap1, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR038851, Rap1 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAP1A_HUMAN | |
Accessioni | P62834Primary (citable) accession number: P62834 Secondary accession number(s): P10113 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | August 16, 2004 | |
Last modified: | April 7, 2021 | |
This is version 184 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families