UniProtKB - P62812 (GBRA1_MOUSE)
Gamma-aminobutyric acid receptor subunit alpha-1
Gabra1
Functioni
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 272 | AgonistBy similarity | 1 |
GO - Molecular functioni
- chloride channel activity Source: GO_Central
- diazepam binding Source: MGI
- GABA-A receptor activity Source: InterPro
- GABA-gated chloride ion channel activity Source: MGI
- inhibitory extracellular ligand-gated ion channel activity Source: GO_Central
- neurotransmitter receptor activity Source: GO_Central
- transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential Source: SynGO
GO - Biological processi
- cellular response to histamine Source: MGI
- chemical synaptic transmission Source: GO_Central
- chloride transmembrane transport Source: MGI
- gamma-aminobutyric acid signaling pathway Source: GO_Central
- inhibitory synapse assembly Source: UniProtKB
- ion transmembrane transport Source: GO_Central
- nervous system process Source: GO_Central
- regulation of membrane potential Source: GO_Central
- regulation of postsynaptic membrane potential Source: SynGO
- signal transduction Source: GO_Central
- synaptic transmission, GABAergic Source: MGI
Keywordsi
Molecular function | Chloride channel, Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Ligand | Chloride |
Enzyme and pathway databases
Reactomei | R-MMU-977443, GABA receptor activation |
Names & Taxonomyi
Protein namesi | Recommended name: Gamma-aminobutyric acid receptor subunit alpha-1Alternative name(s): GABA(A) receptor subunit alpha-1 |
Gene namesi | Name:Gabra1 Synonyms:Gabra-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95613, Gabra1 |
Subcellular locationi
Plasma membrane
- postsynaptic cell membrane By similarity; Multi-pass membrane protein Sequence analysis
- Cell membrane 2 Publications; Multi-pass membrane protein Sequence analysis
Other locations
- Cytoplasmic vesicle membrane By similarity
Plasma Membrane
- dendrite membrane Source: GO_Central
- integral component of plasma membrane Source: MGI
- integral component of postsynaptic specialization membrane Source: MGI
- plasma membrane Source: UniProtKB
Other locations
- chloride channel complex Source: UniProtKB-KW
- cytoplasmic vesicle membrane Source: UniProtKB-SubCell
- GABA receptor complex Source: MGI
- GABA-A receptor complex Source: UniProtKB
- GABA-ergic synapse Source: SynGO
- membrane Source: MGI
- neuron projection Source: GO_Central
- postsynapse Source: GO_Central
- synapse Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 28 – 250 | ExtracellularCuratedAdd BLAST | 223 | |
Transmembranei | 251 – 272 | HelicalCuratedAdd BLAST | 22 | |
Transmembranei | 278 – 299 | HelicalCuratedAdd BLAST | 22 | |
Transmembranei | 312 – 333 | HelicalCuratedAdd BLAST | 22 | |
Topological domaini | 334 – 420 | CytoplasmicCuratedAdd BLAST | 87 | |
Transmembranei | 421 – 442 | HelicalCuratedAdd BLAST | 22 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Postsynaptic cell membrane, SynapsePathology & Biotechi
Disruption phenotypei
Chemistry databases
ChEMBLi | CHEMBL3139 |
DrugCentrali | P62812 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | Sequence analysisAdd BLAST | 27 | |
ChainiPRO_0000000429 | 28 – 455 | Gamma-aminobutyric acid receptor subunit alpha-1Add BLAST | 428 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 37 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 137 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 165 ↔ 179 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P62812 |
PeptideAtlasi | P62812 |
PRIDEi | P62812 |
PTM databases
GlyConnecti | 2320, 7 N-Linked glycans (1 site) |
GlyGeni | P62812, 2 sites |
iPTMneti | P62812 |
PhosphoSitePlusi | P62812 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000010803, Expressed in cerebellum and 212 other tissues |
ExpressionAtlasi | P62812, baseline and differential |
Genevisiblei | P62812, MM |
Interactioni
Subunit structurei
Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains (By similarity).
Interacts with UBQLN1 (By similarity).
Interacts with TRAK1 (PubMed:16380713).
Interacts with KIF21B (By similarity).
Identified in a complex of 720 kDa composed of LHFPL4, NLGN2, GABRA1, GABRB2, GABRG2 and GABRB3 (By similarity).
Interacts with LHFPL4 (By similarity).
Interacts with NLGN2 (By similarity).
Interacts with SHISA7; interaction leads to the regulation of GABA(A) receptor trafficking, channel deactivation kinetics and pharmacology (PubMed:31601770).
By similarity2 PublicationsProtein-protein interaction databases
BioGRIDi | 199797, 20 interactors |
ComplexPortali | CPX-2979, GABA-A receptor, alpha-1/beta-2/gamma-2 CPX-2983, GABA-A receptor, alpha-1/beta-3/gamma-2 |
DIPi | DIP-32219N |
IntActi | P62812, 2 interactors |
STRINGi | 10090.ENSMUSP00000020707 |
Chemistry databases
BindingDBi | P62812 |
Miscellaneous databases
RNActi | P62812, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P62812 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3642, Eukaryota |
GeneTreei | ENSGT00940000159136 |
HOGENOMi | CLU_010920_2_1_1 |
InParanoidi | P62812 |
OMAi | ELHKPAM |
OrthoDBi | 1057372at2759 |
PhylomeDBi | P62812 |
TreeFami | TF315453 |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006028, GABAA/Glycine_rcpt IPR001390, GABAAa_rcpt IPR005431, GABBAa1_rcpt IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR01079, GABAARALPHA PR01614, GABAARALPHA1 PR00253, GABAARECEPTR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MKKSRGLSDY LWAWTLILST LSGRSYGQPS QDELKDNTTV FTRILDRLLD
60 70 80 90 100
GYDNRLRPGL GERVTEVKTD IFVTSFGPVS DHDMEYTIDV FFRQSWKDER
110 120 130 140 150
LKFKGPMTVL RLNNLMASKI WTPDTFFHNG KKSVAHNMTM PNKLLRITED
160 170 180 190 200
GTLLYTMRLT VRAECPMHLE DFPMDAHACP LKFGSYAYTR AEVVYEWTRE
210 220 230 240 250
PARSVVVAED GSRLNQYDLL GQTVDSGIVQ SSTGEYVVMT THFHLKRKIG
260 270 280 290 300
YFVIQTYLPC IMTVILSQVS FWLNRESVPA RTVFGVTTVL TMTTLSISAR
310 320 330 340 350
NSLPKVAYAT AMDWFIAVCY AFVFSALIEF ATVNYFTKRG YAWDGKSVVP
360 370 380 390 400
EKPKKVKDPL IKKNNTYAPT ATSYTPNLAR GDPGLATIAK SATIEPKEVK
410 420 430 440 450
PETKPPEPKK TFNSVSKIDR LSRIAFPLLF GIFNLVYWAT YLNREPQLKA
PTPHQ
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0U1RPE4 | A0A0U1RPE4_MOUSE | Gamma-aminobutyric acid receptor su... | Gabra1 | 302 | Annotation score: | ||
E9Q2L8 | E9Q2L8_MOUSE | Gamma-aminobutyric acid receptor su... | Gabra1 | 91 | Annotation score: | ||
A0A140LJE7 | A0A140LJE7_MOUSE | Gamma-aminobutyric acid receptor su... | Gabra1 | 167 | Annotation score: | ||
E9PXL4 | E9PXL4_MOUSE | Gamma-aminobutyric acid receptor su... | Gabra1 | 132 | Annotation score: | ||
A0A0U1RQ83 | A0A0U1RQ83_MOUSE | Gamma-aminobutyric acid receptor su... | Gabra1 | 114 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86566 mRNA Translation: AAB59634.1 M63436 mRNA Translation: AAA37654.1 X61430 mRNA Translation: CAA43672.1 |
CCDSi | CCDS24553.1 |
PIRi | A39062 |
RefSeqi | NP_034380.1, NM_010250.5 XP_006532259.1, XM_006532196.3 XP_017169748.1, XM_017314259.1 XP_017169749.1, XM_017314260.1 |
Genome annotation databases
Ensembli | ENSMUST00000020707; ENSMUSP00000020707; ENSMUSG00000010803 ENSMUST00000205546; ENSMUSP00000146133; ENSMUSG00000010803 |
GeneIDi | 14394 |
KEGGi | mmu:14394 |
UCSCi | uc007imf.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86566 mRNA Translation: AAB59634.1 M63436 mRNA Translation: AAA37654.1 X61430 mRNA Translation: CAA43672.1 |
CCDSi | CCDS24553.1 |
PIRi | A39062 |
RefSeqi | NP_034380.1, NM_010250.5 XP_006532259.1, XM_006532196.3 XP_017169748.1, XM_017314259.1 XP_017169749.1, XM_017314260.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5OSA | X-ray | 2.75 | A/B/C/D/E | 250-338 | [»] | |
A/B/C/D/E | 417-455 | [»] | ||||
5OSB | X-ray | 3.80 | A/B/C/D/E | 250-338 | [»] | |
A/B/C/D/E | 417-455 | [»] | ||||
5OSC | X-ray | 3.10 | A/B/C/D/E | 250-338 | [»] | |
A/B/C/D/E | 417-455 | [»] | ||||
SMRi | P62812 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 199797, 20 interactors |
ComplexPortali | CPX-2979, GABA-A receptor, alpha-1/beta-2/gamma-2 CPX-2983, GABA-A receptor, alpha-1/beta-3/gamma-2 |
DIPi | DIP-32219N |
IntActi | P62812, 2 interactors |
STRINGi | 10090.ENSMUSP00000020707 |
Chemistry databases
BindingDBi | P62812 |
ChEMBLi | CHEMBL3139 |
DrugCentrali | P62812 |
PTM databases
GlyConnecti | 2320, 7 N-Linked glycans (1 site) |
GlyGeni | P62812, 2 sites |
iPTMneti | P62812 |
PhosphoSitePlusi | P62812 |
Proteomic databases
PaxDbi | P62812 |
PeptideAtlasi | P62812 |
PRIDEi | P62812 |
Protocols and materials databases
ABCDi | P62812, 2 sequenced antibodies |
Antibodypediai | 4533, 473 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000020707; ENSMUSP00000020707; ENSMUSG00000010803 ENSMUST00000205546; ENSMUSP00000146133; ENSMUSG00000010803 |
GeneIDi | 14394 |
KEGGi | mmu:14394 |
UCSCi | uc007imf.2, mouse |
Organism-specific databases
CTDi | 2554 |
MGIi | MGI:95613, Gabra1 |
Phylogenomic databases
eggNOGi | KOG3642, Eukaryota |
GeneTreei | ENSGT00940000159136 |
HOGENOMi | CLU_010920_2_1_1 |
InParanoidi | P62812 |
OMAi | ELHKPAM |
OrthoDBi | 1057372at2759 |
PhylomeDBi | P62812 |
TreeFami | TF315453 |
Enzyme and pathway databases
Reactomei | R-MMU-977443, GABA receptor activation |
Miscellaneous databases
BioGRID-ORCSi | 14394, 0 hits in 17 CRISPR screens |
ChiTaRSi | Gabra1, mouse |
PROi | PR:P62812 |
RNActi | P62812, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000010803, Expressed in cerebellum and 212 other tissues |
ExpressionAtlasi | P62812, baseline and differential |
Genevisiblei | P62812, MM |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006028, GABAA/Glycine_rcpt IPR001390, GABAAa_rcpt IPR005431, GABBAa1_rcpt IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR01079, GABAARALPHA PR01614, GABAARALPHA1 PR00253, GABAARECEPTR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GBRA1_MOUSE | |
Accessioni | P62812Primary (citable) accession number: P62812 Secondary accession number(s): P18504 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | August 16, 2004 | |
Last modified: | February 10, 2021 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families