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Protein

Histone H4

Gene

H4-I

more
Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi17 – 215

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

ReactomeiR-GGA-212300 PRC2 methylates histones and DNA
R-GGA-2299718 Condensation of Prophase Chromosomes
R-GGA-2559580 Oxidative Stress Induced Senescence
R-GGA-3214841 PKMTs methylate histone lysines
R-GGA-3214842 HDMs demethylate histones
R-GGA-3214847 HATs acetylate histones
R-GGA-3214858 RMTs methylate histone arginines
R-GGA-4551638 SUMOylation of chromatin organization proteins
R-GGA-5250924 B-WICH complex positively regulates rRNA expression
R-GGA-5578749 Transcriptional regulation by small RNAs
R-GGA-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-GGA-5693571 Nonhomologous End-Joining (NHEJ)
R-GGA-5693607 Processing of DNA double-strand break ends
R-GGA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-GGA-69473 G2/M DNA damage checkpoint
R-GGA-73728 RNA Polymerase I Promoter Opening
R-GGA-73777 RNA Polymerase I Chain Elongation
R-GGA-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-GGA-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H4
Gene namesi
Name:H4-I
AND
Name:H4-II
AND
Name:H4-III
AND
Name:H4-IV
AND
Name:H4-V
AND
Name:H4-VI
AND
Name:H4-VII
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001582962 – 103Histone H4Add BLAST102

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei4Asymmetric dimethylarginine; by PRMT1; alternateBy similarity1
Modified residuei4Citrulline; alternateBy similarity1
Modified residuei4Omega-N-methylarginine; by PRMT1; alternateBy similarity1
Modified residuei4Symmetric dimethylarginine; by PRMT5 and PRMT7; alternateBy similarity1
Modified residuei6N6-(2-hydroxyisobutyryl)lysine; alternateBy similarity1
Modified residuei6N6-acetyllysineBy similarity1
Modified residuei6N6-butyryllysine; alternateBy similarity1
Modified residuei9N6-(2-hydroxyisobutyryl)lysine; alternateBy similarity1
Modified residuei9N6-acetyllysineBy similarity1
Modified residuei9N6-butyryllysine; alternateBy similarity1
Modified residuei9N6-propionyllysine; alternateBy similarity1
Modified residuei13N6-(2-hydroxyisobutyryl)lysine; alternateBy similarity1
Modified residuei13N6-acetyllysineBy similarity1
Modified residuei13N6-butyryllysine; alternateBy similarity1
Modified residuei17N6-(2-hydroxyisobutyryl)lysine; alternateBy similarity1
Modified residuei17N6-acetyllysineBy similarity1
Modified residuei17N6-butyryllysine; alternateBy similarity1
Modified residuei17N6-propionyllysine; alternateBy similarity1
Modified residuei21N6,N6,N6-trimethyllysine; alternateBy similarity1
Modified residuei21N6,N6-dimethyllysine; alternateBy similarity1
Modified residuei21N6-methylated lysineBy similarity1
Modified residuei21N6-methyllysine; alternateBy similarity1
Modified residuei32N6-(2-hydroxyisobutyryl)lysine; alternateBy similarity1
Modified residuei32N6-acetyllysineBy similarity1
Modified residuei32N6-butyryllysine; alternateBy similarity1
Modified residuei32N6-propionyllysine; alternateBy similarity1
Modified residuei32N6-succinyllysine; alternateBy similarity1
Modified residuei45N6-(2-hydroxyisobutyryl)lysine; alternateBy similarity1
Modified residuei45N6-butyryllysine; alternateBy similarity1
Modified residuei45N6-propionyllysine; alternateBy similarity1
Modified residuei48Phosphoserine; by PAK2By similarity1
Modified residuei52PhosphotyrosineBy similarity1
Modified residuei60N6-(2-hydroxyisobutyryl)lysineBy similarity1
Modified residuei60N6-acetyllysineBy similarity1
Modified residuei78N6-(2-hydroxyisobutyryl)lysine; alternateBy similarity1
Modified residuei78N6-butyryllysine; alternateBy similarity1
Modified residuei78N6-propionyllysine; alternateBy similarity1
Modified residuei78N6-succinyllysineBy similarity1
Modified residuei80N6-(2-hydroxyisobutyryl)lysine; alternateBy similarity1
Modified residuei80N6-acetyllysineBy similarity1
Modified residuei80N6-butyryllysine; alternateBy similarity1
Modified residuei80N6-propionyllysine; alternateBy similarity1
Modified residuei89PhosphotyrosineBy similarity1
Modified residuei92N6-(2-hydroxyisobutyryl)lysine; alternateBy similarity1
Modified residuei92N6-acetyllysine; alternateBy similarity1
Modified residuei92N6-butyryllysine; alternateBy similarity1
Modified residuei92N6-propionyllysine; alternateBy similarity1
Modified residuei92N6-succinyllysine; alternateBy similarity1
Cross-linki92Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity

Post-translational modificationi

Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.By similarity
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.By similarity
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity).By similarity
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by KMT5B and KMT5C and induces gene silencing (By similarity).By similarity
Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4 (By similarity).By similarity
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me) (By similarity).By similarity
Sumoylated, which is associated with transcriptional repression.By similarity
Butyrylation of histones marks active promoters and competes with histone acetylation.By similarity

Keywords - PTMi

Acetylation, Citrullination, Hydroxylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP62801
PRIDEiP62801

Expressioni

Gene expression databases

BgeeiENSGALG00000025786 Expressed in 10 organ(s), highest expression level in liver

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi679227, 7 interactors
IntActiP62801, 2 interactors
STRINGi9031.ENSGALP00000037292

Structurei

Secondary structure

1103
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP62801
SMRiP62801
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62801

Family & Domainsi

Sequence similaritiesi

Belongs to the histone H4 family.Curated

Phylogenomic databases

eggNOGiKOG3467 Eukaryota
COG2036 LUCA
GeneTreeiENSGT00760000119019
HOVERGENiHBG051878
InParanoidiP62801
KOiK11254
OMAiHINGITK
OrthoDBiEOG091G0XGD
PhylomeDBiP62801

Family and domain databases

CDDicd00076 H4, 1 hit
Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR035425 CENP-T/H4_C
IPR009072 Histone-fold
IPR001951 Histone_H4
IPR019809 Histone_H4_CS
IPR004823 TAF_TATA-bd
PfamiView protein in Pfam
PF15511 CENP-T_C, 1 hit
PRINTSiPR00623 HISTONEH4
SMARTiView protein in SMART
SM00417 H4, 1 hit
SM00803 TAF, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00047 HISTONE_H4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62801-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLAR RGGVKRISGL
60 70 80 90 100
IYEETRGVLK VFLENVIRDA VTYTEHAKRK TVTAMDVVYA LKRQGRTLYG

FGG
Length:103
Mass (Da):11,367
Last modified:January 23, 2007 - v2
Checksum:iA9E5DFD3F8B97598
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02218 Genomic DNA Translation: CAA26137.1
X02218 Genomic DNA Translation: CAA26140.1
J00866 Genomic DNA No translation available.
M74533 Genomic DNA Translation: AAA73091.1
M74534 Genomic DNA Translation: AAA73092.1
U37575 Genomic DNA Translation: AAC59999.1
U37575 Genomic DNA Translation: AAC60001.1
PIRiA02640 HSCH4
JH0507
RefSeqiNP_001032932.1, NM_001037843.1
NP_001032934.1, NM_001037845.1
NP_001268414.1, NM_001281485.1
XP_001233180.1, XM_001233179.4
XP_003640418.1, XM_003640370.3
XP_004937726.1, XM_004937669.2
XP_425458.2, XM_425458.5
UniGeneiGga.39882

Genome annotation databases

EnsembliENSGALT00000046233; ENSGALP00000041526; ENSGALG00000025786
ENSGALT00000049482; ENSGALP00000054690; ENSGALG00000032272
ENSGALT00000053533; ENSGALP00000054121; ENSGALG00000032933
ENSGALT00000066061; ENSGALP00000045681; ENSGALG00000032198
ENSGALT00000067674; ENSGALP00000051551; ENSGALG00000035445
ENSGALT00000070686; ENSGALP00000058410; ENSGALG00000037322
ENSGALT00000079865; ENSGALP00000056887; ENSGALG00000041398
ENSGALT00000080967; ENSGALP00000049875; ENSGALG00000042491
GeneIDi100858049
100858319
417946
417950
427884
770005
770142
KEGGigga:100858049
gga:100858319
gga:417946
gga:417950
gga:427884
gga:770005
gga:770142

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02218 Genomic DNA Translation: CAA26137.1
X02218 Genomic DNA Translation: CAA26140.1
J00866 Genomic DNA No translation available.
M74533 Genomic DNA Translation: AAA73091.1
M74534 Genomic DNA Translation: AAA73092.1
U37575 Genomic DNA Translation: AAC59999.1
U37575 Genomic DNA Translation: AAC60001.1
PIRiA02640 HSCH4
JH0507
RefSeqiNP_001032932.1, NM_001037843.1
NP_001032934.1, NM_001037845.1
NP_001268414.1, NM_001281485.1
XP_001233180.1, XM_001233179.4
XP_003640418.1, XM_003640370.3
XP_004937726.1, XM_004937669.2
XP_425458.2, XM_425458.5
UniGeneiGga.39882

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EQZX-ray2.50D/H1-103[»]
1HIOX-ray3.10D28-103[»]
1HQ3X-ray2.15D/H1-103[»]
1TZYX-ray1.90D/H1-103[»]
2AROX-ray2.10D/H1-103[»]
2HIOX-ray3.10D1-103[»]
3C9Kelectron microscopy20.00D/H2-103[»]
ProteinModelPortaliP62801
SMRiP62801
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi679227, 7 interactors
IntActiP62801, 2 interactors
STRINGi9031.ENSGALP00000037292

Proteomic databases

PaxDbiP62801
PRIDEiP62801

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000046233; ENSGALP00000041526; ENSGALG00000025786
ENSGALT00000049482; ENSGALP00000054690; ENSGALG00000032272
ENSGALT00000053533; ENSGALP00000054121; ENSGALG00000032933
ENSGALT00000066061; ENSGALP00000045681; ENSGALG00000032198
ENSGALT00000067674; ENSGALP00000051551; ENSGALG00000035445
ENSGALT00000070686; ENSGALP00000058410; ENSGALG00000037322
ENSGALT00000079865; ENSGALP00000056887; ENSGALG00000041398
ENSGALT00000080967; ENSGALP00000049875; ENSGALG00000042491
GeneIDi100858049
100858319
417946
417950
427884
770005
770142
KEGGigga:100858049
gga:100858319
gga:417946
gga:417950
gga:427884
gga:770005
gga:770142

Organism-specific databases

CTDi100858049
417946
554313
770142
8294
8360
8366

Phylogenomic databases

eggNOGiKOG3467 Eukaryota
COG2036 LUCA
GeneTreeiENSGT00760000119019
HOVERGENiHBG051878
InParanoidiP62801
KOiK11254
OMAiHINGITK
OrthoDBiEOG091G0XGD
PhylomeDBiP62801

Enzyme and pathway databases

ReactomeiR-GGA-212300 PRC2 methylates histones and DNA
R-GGA-2299718 Condensation of Prophase Chromosomes
R-GGA-2559580 Oxidative Stress Induced Senescence
R-GGA-3214841 PKMTs methylate histone lysines
R-GGA-3214842 HDMs demethylate histones
R-GGA-3214847 HATs acetylate histones
R-GGA-3214858 RMTs methylate histone arginines
R-GGA-4551638 SUMOylation of chromatin organization proteins
R-GGA-5250924 B-WICH complex positively regulates rRNA expression
R-GGA-5578749 Transcriptional regulation by small RNAs
R-GGA-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-GGA-5693571 Nonhomologous End-Joining (NHEJ)
R-GGA-5693607 Processing of DNA double-strand break ends
R-GGA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-GGA-69473 G2/M DNA damage checkpoint
R-GGA-73728 RNA Polymerase I Promoter Opening
R-GGA-73777 RNA Polymerase I Chain Elongation
R-GGA-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-GGA-9018519 Estrogen-dependent gene expression

Miscellaneous databases

EvolutionaryTraceiP62801
PROiPR:P62801

Gene expression databases

BgeeiENSGALG00000025786 Expressed in 10 organ(s), highest expression level in liver

Family and domain databases

CDDicd00076 H4, 1 hit
Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR035425 CENP-T/H4_C
IPR009072 Histone-fold
IPR001951 Histone_H4
IPR019809 Histone_H4_CS
IPR004823 TAF_TATA-bd
PfamiView protein in Pfam
PF15511 CENP-T_C, 1 hit
PRINTSiPR00623 HISTONEH4
SMARTiView protein in SMART
SM00417 H4, 1 hit
SM00803 TAF, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00047 HISTONE_H4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiH4_CHICK
AccessioniPrimary (citable) accession number: P62801
Secondary accession number(s): P02304, P02305
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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