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Protein

Rho-related GTP-binding protein RhoB

Gene

Rhob

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 19GTPBy similarity8
Nucleotide bindingi59 – 63GTPBy similarity5
Nucleotide bindingi117 – 120GTPBy similarity4

GO - Molecular functioni

  • GDP binding Source: MGI
  • GTPase activity Source: GO_Central
  • GTP binding Source: GO_Central
  • protein kinase binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processAngiogenesis, Apoptosis, Cell adhesion, Differentiation, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-114604 GPVI-mediated activation cascade
R-MMU-194840 Rho GTPase cycle
R-MMU-416482 G alpha (12/13) signalling events
R-MMU-416572 Sema4D induced cell migration and growth-cone collapse
R-MMU-5625740 RHO GTPases activate PKNs
R-MMU-5625900 RHO GTPases activate CIT
R-MMU-5627117 RHO GTPases Activate ROCKs
R-MMU-5663220 RHO GTPases Activate Formins

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoB
Gene namesi
Name:Rhob
Synonyms:Arhb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:107949 Rhob

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000304191 – 193Rho-related GTP-binding protein RhoBAdd BLAST193
PropeptideiPRO_0000030420194 – 196Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei154PhosphotyrosineCombined sources1
Lipidationi189S-palmitoyl cysteineBy similarity1
Lipidationi192S-palmitoyl cysteineBy similarity1
Modified residuei193Cysteine methyl esterBy similarity1
Lipidationi193S-farnesyl cysteine; in plasma membrane formBy similarity1
Lipidationi193S-geranylgeranyl cysteine; in endosomal formBy similarity1

Post-translational modificationi

Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP62746
PeptideAtlasiP62746
PRIDEiP62746

PTM databases

iPTMnetiP62746
PhosphoSitePlusiP62746
SwissPalmiP62746

Expressioni

Inductioni

By UV irradiation, N-methyl-N-nitrosourea, cisplatin, cyclohexamide and serum stimulation.2 Publications

Gene expression databases

BgeeiENSMUSG00000054364 Expressed in 312 organ(s), highest expression level in molar tooth
CleanExiMM_RHOB
ExpressionAtlasiP62746 baseline and differential
GenevisibleiP62746 MM

Interactioni

Subunit structurei

Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARGGEF3 (By similarity). Interacts with RTKN (PubMed:8662891). Interacts with AKAP13. Interacts with RIPOR1 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiP62746, 2 interactors
MINTiP62746
STRINGi10090.ENSMUSP00000067013

Structurei

3D structure databases

ProteinModelPortaliP62746
SMRiP62746
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi34 – 42Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000119020
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiP62746
KOiK07856
OMAiHIELALW
OrthoDBiEOG091G0QVS
PhylomeDBiP62746
TreeFamiTF300837

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62746-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG
60 70 80 90 100
KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV
110 120 130 140 150
PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR
160 170 180 190
IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCCKVL
Length:196
Mass (Da):22,123
Last modified:August 1, 1988 - v1
Checksum:iCCE6FD53AE00CD83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99963 Genomic DNA Translation: CAA68228.1
AF481943 mRNA Translation: AAL89687.1
BC018275 mRNA Translation: AAH18275.1
AK013784 mRNA Translation: BAB28993.1
CCDSiCCDS25799.1
PIRiJC5075
RefSeqiNP_031509.1, NM_007483.2
UniGeneiMm.687

Genome annotation databases

EnsembliENSMUST00000067384; ENSMUSP00000067013; ENSMUSG00000054364
GeneIDi11852
KEGGimmu:11852
UCSCiuc007mzp.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99963 Genomic DNA Translation: CAA68228.1
AF481943 mRNA Translation: AAL89687.1
BC018275 mRNA Translation: AAH18275.1
AK013784 mRNA Translation: BAB28993.1
CCDSiCCDS25799.1
PIRiJC5075
RefSeqiNP_031509.1, NM_007483.2
UniGeneiMm.687

3D structure databases

ProteinModelPortaliP62746
SMRiP62746
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP62746, 2 interactors
MINTiP62746
STRINGi10090.ENSMUSP00000067013

PTM databases

iPTMnetiP62746
PhosphoSitePlusiP62746
SwissPalmiP62746

Proteomic databases

PaxDbiP62746
PeptideAtlasiP62746
PRIDEiP62746

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067384; ENSMUSP00000067013; ENSMUSG00000054364
GeneIDi11852
KEGGimmu:11852
UCSCiuc007mzp.1 mouse

Organism-specific databases

CTDi388
MGIiMGI:107949 Rhob

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000119020
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiP62746
KOiK07856
OMAiHIELALW
OrthoDBiEOG091G0QVS
PhylomeDBiP62746
TreeFamiTF300837

Enzyme and pathway databases

ReactomeiR-MMU-114604 GPVI-mediated activation cascade
R-MMU-194840 Rho GTPase cycle
R-MMU-416482 G alpha (12/13) signalling events
R-MMU-416572 Sema4D induced cell migration and growth-cone collapse
R-MMU-5625740 RHO GTPases activate PKNs
R-MMU-5625900 RHO GTPases activate CIT
R-MMU-5627117 RHO GTPases Activate ROCKs
R-MMU-5663220 RHO GTPases Activate Formins

Miscellaneous databases

PROiPR:P62746
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000054364 Expressed in 312 organ(s), highest expression level in molar tooth
CleanExiMM_RHOB
ExpressionAtlasiP62746 baseline and differential
GenevisibleiP62746 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRHOB_MOUSE
AccessioniPrimary (citable) accession number: P62746
Secondary accession number(s): P01121, Q9CUV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1988
Last modified: November 7, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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