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Protein

Rho-related GTP-binding protein RhoB

Gene

RHOB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.5 Publications

Miscellaneous

RHOB is one of the targets of farnesyltransferase inhibitors which are currently under investigation as cancer therapeutics. These elevate the levels of geranylgeranylated RHOB and cause mislocalization, leading to apoptosis and antineoplastic effects.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 19GTPBy similarity8
Nucleotide bindingi59 – 63GTPBy similarity5
Nucleotide bindingi117 – 120GTPBy similarity4

GO - Molecular functioni

  • GDP binding Source: Ensembl
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB

GO - Biological processi

  • angiogenesis Source: UniProtKB-KW
  • apoptotic process Source: UniProtKB
  • cell adhesion Source: UniProtKB
  • cell differentiation Source: UniProtKB-KW
  • cellular response to hydrogen peroxide Source: UniProtKB
  • cellular response to ionizing radiation Source: UniProtKB
  • endosome to lysosome transport Source: UniProtKB
  • endothelial tube morphogenesis Source: BHF-UCL
  • G-protein coupled receptor signaling pathway Source: Reactome
  • intracellular protein transport Source: Ensembl
  • mitotic cytokinesis Source: UniProtKB
  • negative regulation of cell cycle Source: UniProtKB
  • negative regulation of cell migration Source: BHF-UCL
  • positive regulation of angiogenesis Source: UniProtKB
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of endothelial cell migration Source: BHF-UCL
  • regulation of cell migration Source: BHF-UCL
  • regulation of small GTPase mediated signal transduction Source: Reactome
  • Rho protein signal transduction Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein
Biological processAngiogenesis, Apoptosis, Cell adhesion, Differentiation, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-114604 GPVI-mediated activation cascade
R-HSA-194840 Rho GTPase cycle
R-HSA-416482 G alpha (12/13) signalling events
R-HSA-416572 Sema4D induced cell migration and growth-cone collapse
R-HSA-5625740 RHO GTPases activate PKNs
R-HSA-5625900 RHO GTPases activate CIT
R-HSA-5627117 RHO GTPases Activate ROCKs
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-5666185 RHO GTPases Activate Rhotekin and Rhophilins
SignaLinkiP62745
SIGNORiP62745

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoB
Alternative name(s):
Rho cDNA clone 6
Short name:
h6
Gene namesi
Name:RHOB
Synonyms:ARH6, ARHB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000143878.9
HGNCiHGNC:668 RHOB
MIMi165370 gene
neXtProtiNX_P62745

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14G → V: No effect on internalization of EGF receptor but decreases trafficking of receptor to the lysosome with associated accumulation in late endosomes. 1 Publication1
Mutagenesisi39F → G: Abolishes binding to PKN1 and trafficking of EGF receptor. 1 Publication1
Mutagenesisi189C → S: No effect on prenylation. Reduced palmitoylation. Abolishes palmitoylation; when associated with S-192. 1 Publication1
Mutagenesisi192C → S: Reduced geranylgeranylation but no effect on farnesylation. Reduced palmitoylation. Abolishes palmitoylation; when associated with S-189. 2 Publications1
Mutagenesisi193C → S: Abolishes methylation, palmitoylation and prenylation. 2 Publications1
Mutagenesisi194K → L: No effect on palmitoylation or prenylation. 1 Publication1

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNETi388
OpenTargetsiENSG00000143878
PharmGKBiPA24950

Chemistry databases

ChEMBLiCHEMBL1795102
DrugBankiDB00083 Botulinum Toxin Type A

Polymorphism and mutation databases

BioMutaiRHOB
DMDMi51338601

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000304171 – 193Rho-related GTP-binding protein RhoBAdd BLAST193
PropeptideiPRO_0000030418194 – 196Removed in mature form3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi34O-linked (GlcNAc) tyrosine; by Photorhabdus PAU_022301 Publication1
Modified residuei41ADP-ribosylasparagine; by botulinum toxinBy similarity1
Modified residuei154PhosphotyrosineBy similarity1
Lipidationi189S-palmitoyl cysteine1 Publication1
Lipidationi192S-palmitoyl cysteine1 Publication1
Modified residuei193Cysteine methyl ester1 Publication1
Lipidationi193S-farnesyl cysteine; in plasma membrane form2 Publications1
Lipidationi193S-geranylgeranyl cysteine; in endosomal form2 Publications1

Post-translational modificationi

Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.2 Publications
(Microbial infection) Glycosylated at Tyr-34 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rho and leads to actin disassembly.1 Publication

Keywords - PTMi

ADP-ribosylation, Glycoprotein, Lipoprotein, Methylation, Palmitate, Phosphoprotein, Prenylation

Proteomic databases

EPDiP62745
MaxQBiP62745
PaxDbiP62745
PeptideAtlasiP62745
PRIDEiP62745
ProteomicsDBi57420
TopDownProteomicsiP62745

PTM databases

iPTMnetiP62745
PhosphoSitePlusiP62745
SwissPalmiP62745

Expressioni

Inductioni

Up-regulated by DNA damaging agents like H2O2 or ionizing radiation (IR).1 Publication

Gene expression databases

BgeeiENSG00000143878
CleanExiHS_RHOB
GenevisibleiP62745 HS

Interactioni

Subunit structurei

Binds ROCK1 and ROCK2 (By similarity). Also binds PKN1/PRK1 (PubMed:9478917). Interacts with ARGGEF3 (PubMed:12221096). Interacts with RTKN (By similarity). Interacts with AKAP13 (PubMed:11546812). Interacts with RIPOR1 (PubMed:27807006).By similarity4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi106881, 20 interactors
IntActiP62745, 24 interactors
MINTiP62745
STRINGi9606.ENSP00000272233

Structurei

Secondary structure

1196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 12Combined sources9
Helixi18 – 27Combined sources10
Beta strandi41 – 48Combined sources8
Beta strandi51 – 59Combined sources9
Turni67 – 69Combined sources3
Helixi70 – 73Combined sources4
Beta strandi79 – 85Combined sources7
Helixi89 – 97Combined sources9
Helixi99 – 106Combined sources8
Beta strandi112 – 117Combined sources6
Helixi119 – 123Combined sources5
Helixi125 – 133Combined sources9
Helixi141 – 150Combined sources10
Beta strandi154 – 158Combined sources5
Turni161 – 163Combined sources3
Helixi167 – 179Combined sources13

3D structure databases

ProteinModelPortaliP62745
SMRiP62745
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62745

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi34 – 42Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000119020
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiP62745
KOiK07856
OMAiWEVFETA
OrthoDBiEOG091G0QVS
PhylomeDBiP62745
TreeFamiTF300837

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG
60 70 80 90 100
KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV
110 120 130 140 150
PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR
160 170 180 190
IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCCKVL
Length:196
Mass (Da):22,123
Last modified:August 1, 1988 - v1
Checksum:iCCE6FD53AE00CD83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06820 mRNA Translation: CAA29968.1
AF498971 mRNA Translation: AAM21118.1
CR542272 mRNA Translation: CAG47068.1
AK124398 mRNA Translation: BAG54035.1
AK312487 mRNA Translation: BAG35389.1
BT019546 mRNA Translation: AAV38353.1
BT019547 mRNA Translation: AAV38354.1
AC023137 Genomic DNA Translation: AAY24345.1
CH471053 Genomic DNA Translation: EAX00819.1
CH471053 Genomic DNA Translation: EAX00820.1
BC066954 mRNA Translation: AAH66954.1
M12174 mRNA Translation: AAA36565.1
BK001232 mRNA Translation: DAA01138.1
BK001671 Genomic DNA Translation: DAA01912.1
CCDSiCCDS1699.1
PIRiA01372 TVHURH
RefSeqiNP_004031.1, NM_004040.3
UniGeneiHs.502876

Genome annotation databases

EnsembliENST00000272233; ENSP00000272233; ENSG00000143878
GeneIDi388
KEGGihsa:388
UCSCiuc002rdv.4 human

Similar proteinsi

Entry informationi

Entry nameiRHOB_HUMAN
AccessioniPrimary (citable) accession number: P62745
Secondary accession number(s): B2R692
, P01121, Q5U0H6, Q7RTN5, Q7RTR9, Q9CUV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1988
Last modified: June 20, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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