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Protein

Two-component response regulator ARR12

Gene

ARR12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]GATT-3'. Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Could directly activate some type-A response regulators in response to cytokinins. Involved in the root-meristem size determination through the regulation of cell differentiation (PubMed:17363254). Involved in activating SHY2 during meristem growth and controls PIN expression via activation of SHY2 (PubMed:20605455).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi197 – 247Myb-like GARPPROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA-binding transcription factor activity Source: TAIR
  • phosphorelay response regulator activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCytokinin signaling pathway, Transcription, Transcription regulation, Two-component regulatory system

Names & Taxonomyi

Protein namesi
Recommended name:
Two-component response regulator ARR12
Gene namesi
Name:ARR12
Ordered Locus Names:At2g25180
ORF Names:F13D4.140
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G25180
TAIRilocus:2040194 AT2G25180

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001322971 – 596Two-component response regulator ARR12Add BLAST596

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei694-aspartylphosphatePROSITE-ProRule annotation1

Post-translational modificationi

Two-component system major event consists of a His-to-Asp phosphorelay between a sensor histidine kinase (HK) and a response regulator (RR). In plants, the His-to-Asp phosphorelay involves an additional intermediate named Histidine-containing phosphotransfer protein (HPt). This multistep phosphorelay consists of a His-Asp-His-Asp sequential transfer of a phosphate group between first an His and an Asp of the HK protein, followed by the transfer to a conserved His of the HPt protein and finally the transfer to an Asp in the receiver domain of the RR protein.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP62598
PRIDEiP62598

Expressioni

Tissue specificityi

Detected in the whole plant. Predominantly expressed in leaves. Expressed at the root transition zone (PubMed:17363254).2 Publications

Developmental stagei

Detected immediately after seed germination.1 Publication

Gene expression databases

ExpressionAtlasiP62598 baseline and differential
GenevisibleiP62598 AT

Interactioni

Subunit structurei

Binds the target DNA as a monomer.By similarity

Protein-protein interaction databases

BioGridi2408, 7 interactors
IntActiP62598, 2 interactors
STRINGi3702.AT2G25180.1

Structurei

3D structure databases

ProteinModelPortaliP62598
SMRiP62598
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 133Response regulatoryPROSITE-ProRule annotationAdd BLAST116

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi194 – 197Nuclear localization signalSequence analysis4

Sequence similaritiesi

Belongs to the ARR family. Type-B subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IMXM Eukaryota
COG2204 LUCA
HOGENOMiHOG000238840
InParanoidiP62598
KOiK14491
OMAiMPLEFDQ
OrthoDBiEOG09360B7P

Family and domain databases

CDDicd00156 REC, 1 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR009057 Homeobox-like_sf
IPR017930 Myb_dom
IPR006447 Myb_dom_plants
IPR017053 Response_reg_B-typ_pln
IPR001005 SANT/Myb
IPR001789 Sig_transdc_resp-reg_receiver
PfamiView protein in Pfam
PF00249 Myb_DNA-binding, 1 hit
PF00072 Response_reg, 1 hit
PIRSFiPIRSF036392 RR_ARR_type-B, 1 hit
SMARTiView protein in SMART
SM00448 REC, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF52172 SSF52172, 1 hit
TIGRFAMsiTIGR01557 myb_SHAQKYF, 1 hit
PROSITEiView protein in PROSITE
PS51294 HTH_MYB, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

P62598-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTVEQNLEAL DQFPVGMRVL AVDDDQTCLK ILESLLRHCQ YHVTTTNQAQ
60 70 80 90 100
KALELLRENK NKFDLVISDV DMPDMDGFKL LELVGLEMDL PVIMLSAHSD
110 120 130 140 150
PKYVMKGVTH GACDYLLKPV RIEELKNIWQ HVVRSRFDKN RGSNNNGDKR
160 170 180 190 200
DGSGNEGVGN SDQNNGKGNR KRKDQYNEDE DEDRDDNDDS CAQKKQRVVW
210 220 230 240 250
TVELHKKFVA AVNQLGYEKA MPKKILDLMN VEKLTRENVA SHLQKFRLYL
260 270 280 290 300
KRISGVANQQ AIMANSELHF MQMNGLDGFH HRPIPVGSGQ YHGGAPAMRS
310 320 330 340 350
FPPNGILGRL NTPSGIGVRS LSSPPAGMFL QNQTDIGKFH HVSSLPLNHS
360 370 380 390 400
DGGNILQGLP MPLEFDQLQT NNNKSRNMNS NKSIAGTSMA FPSFSTQQNS
410 420 430 440 450
LISAPNNNVV VLEGHPQATP PGFPGHQINK RLEHWSNAVS SSTHPPPPAH
460 470 480 490 500
NSNSINHQFD VSPLPHSRPD PLEWNNVSSS YSIPFCDSAN TLSSPALDTT
510 520 530 540 550
NPRAFCRNTD FDSNTNVQPG VFYGPSTDAM ALLSSSNPKE GFVVGQQKLQ
560 570 580 590
SGGFMVADAG SLDDIVNSTM KQEQSQGDLS GGDLGYGGFS SLRTCI
Length:596
Mass (Da):65,626
Last modified:February 22, 2012 - v2
Checksum:iC9E0D3F5EEE1B149
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti414G → S in BE522295 (PubMed:11115876).Curated1
Sequence conflicti426H → T in BE522295 (PubMed:11115876).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002685 Genomic DNA Translation: AEC07667.1
BE522295 mRNA No translation available.
RefSeqiNP_180090.6, NM_128075.7
UniGeneiAt.19775

Genome annotation databases

EnsemblPlantsiAT2G25180.1; AT2G25180.1; AT2G25180
GeneIDi817056
GrameneiAT2G25180.1; AT2G25180.1; AT2G25180
KEGGiath:AT2G25180

Similar proteinsi

Entry informationi

Entry nameiARR12_ARATH
AccessioniPrimary (citable) accession number: P62598
Secondary accession number(s): F4IRL1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: February 22, 2012
Last modified: September 12, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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