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Protein

Beta-lactamase TEM

Gene

bla

more
Organism
Escherichia coli
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.

Miscellaneous

The beta-lactamase present on pBR322 was cloned from plasmid R1 (R7268).

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei68Acyl-ester intermediate1
Active sitei166Proton acceptor1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAntibiotic resistance

Enzyme and pathway databases

BRENDAi3.5.2.6 2026
SABIO-RKiP62593

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase TEM (EC:3.5.2.6)
Alternative name(s):
IRT-4
Penicillinase
TEM-1
TEM-16/CAZ-7
TEM-2
TEM-24/CAZ-6
TEM-3
TEM-4
TEM-5
TEM-6
TEM-8/CAZ-2
Gene namesi
Name:bla
AND
Name:blaT-3
AND
Name:blaT-4
AND
Name:blaT-5
AND
Name:blaT-6
Encoded oniPlasmid R1 (R7268)2 Publications
Plasmid IncFII R100 (NR1)1 Publication
Plasmid R6K1 Publication
Plasmid pUD161 Publication
Plasmid pCFF042 Publications
Plasmid pCFF140 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Pathology & Biotechi

Biotechnological usei

This protein is used as a marker in many commonly used cloning vectors, such as pBR322 and the pUC series.

Chemistry databases

ChEMBLiCHEMBL2065
DrugBankiDB08551 (1R)-1-(2-THIENYLACETYLAMINO)-1-(3-CARBOXYPHENYL)METHYLBORONIC ACID
DB07466 (1R)-2-PHENYLACETAMIDO-2-(3-CARBOXYPHENYL)ETHYL BORONIC ACID
DB07464 1(R)-1-ACETAMIDO-2-(3-CARBOXY-2-HYDROXYPHENYL)ETHYL BORONIC ACID
DB02614 1(R)-1-Acetamido-2-(3-Carboxyphenyl)Ethyl Boronic Acid
DB04430 3-(4-Phenylamino-Phenylamino)-2-(1h-Tetrazol-5-Yl)-Acrylonitrile
DB07599 [(2-AMINO-ALPHA-METHOXYIMINO-4-THIAZOLYLACETYL)AMINO]METHYLBORONIC ACID
DB02841 [(2-Ethoxy-1-Naphthoyl)Amino]Methylboronic Acid
DB02642 [[N-(Benzyloxycarbonyl)Amino]Methyl]Phosphate
DB09060 Avibactam
DB01053 Benzylpenicillin
DB04035 Pinacol[[2-Amino-Alpha-(1-Carboxy-1-Methylethoxyimino)-4-Thiazoleacetyl]Amino]Methaneboronate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 232 PublicationsAdd BLAST23
ChainiPRO_000001697824 – 286Beta-lactamase TEMAdd BLAST263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi75 ↔ 121

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP62593

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
P358042EBI-1031989,EBI-1031985From Streptomyces clavuligerus.

Protein-protein interaction databases

IntActiP62593, 1 interactor

Chemistry databases

BindingDBiP62593

Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP62593
SMRiP62593
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62593

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni232 – 234Substrate binding3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK18698

Family and domain databases

InterProiView protein in InterPro
IPR012338 Beta-lactam/transpept-like
IPR000871 Beta-lactam_class-A
IPR023650 Beta-lactam_class-A_AS
PRINTSiPR00118 BLACTAMASEA
SUPFAMiSSF56601 SSF56601, 1 hit
PROSITEiView protein in PROSITE
PS00146 BETA_LACTAMASE_A, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62593-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN
60 70 80 90 100
SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL
110 120 130 140 150
VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL
160 170 180 190 200
HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA
210 220 230 240 250
SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD
260 270 280
GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW
Length:286
Mass (Da):31,515
Last modified:July 21, 1986 - v1
Checksum:iBB678943BB18934B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti19L → F in TEM-4. 1
Natural varianti37Q → K in TEM-2, TEM-3, TEM-8, TEM-16 and TEM-24. 1
Natural varianti67M → L in IRT-4. 1
Natural varianti102E → K in TEM-3, TEM-4, TEM-6, TEM-8, TEM-16 and TEM-24. 1
Natural varianti162R → H in TEM-6 and TEM-16. 1
Natural varianti162R → S in TEM-5, TEM-8 and TEM-24. 1
Natural varianti235A → T in TEM-5 and TEM-24. 1
Natural varianti236G → S in TEM-3, TEM-4 and TEM-8. 1
Natural varianti237E → K in TEM-5 and TEM-24. 1
Natural varianti261T → M in TEM-4. 1
Natural varianti272N → D in IRT-4. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01749 Genomic DNA Translation: AAB59737.1
V00613 Genomic DNA Translation: CAA23886.1
X64523 Genomic DNA Translation: CAA45828.1
X57972 Genomic DNA Translation: CAA41038.1
X65252 Genomic DNA Translation: CAA46344.1
X65253 Genomic DNA Translation: CAA46345.1
X65254 Genomic DNA Translation: CAA46346.1
U89928 Genomic DNA Translation: AAB64386.1
U66885 Genomic DNA Translation: AAC48875.1
PIRiA93821 PNECP
S30113
RefSeqiNP_943295.1, NC_005248.1
NP_957565.1, NC_005327.1
WP_000027057.1, NZ_UIMY01000047.1
WP_063864949.1, NZ_BGKM01000047.1
WP_063865014.1, NZ_BGLS01000178.1
YP_001096393.1, NC_009132.1
YP_001693174.1, NC_010378.1
YP_001816609.1, NC_010558.1
YP_003108102.1, NC_013120.1
YP_003108210.1, NC_013121.1
YP_003829069.1, NC_014383.1
YP_003829170.1, NC_014384.1
YP_003937675.1, NC_014615.1
YP_004119720.1, NC_014843.1
YP_004119734.1, NC_014843.1
YP_006903139.1, NC_019047.1
YP_006939984.1, NC_018994.1
YP_006940092.1, NC_018995.1
YP_006952181.1, NC_019056.1
YP_006952421.1, NC_019062.1
YP_006952427.1, NC_019063.1
YP_006953479.1, NC_019073.1
YP_006953762.1, NC_019088.1
YP_006953988.1, NC_019091.1
YP_006954235.1, NC_019095.1
YP_007447512.1, NC_020278.2
YP_008864019.1, NC_022992.1
YP_008864686.1, NC_022996.1
YP_008995211.1, NC_023277.2
YP_009060387.1, NC_024960.1
YP_009060444.1, NC_024961.1
YP_009060580.1, NC_024967.1
YP_009061316.1, NC_024977.1
YP_009066523.1, NC_025106.1
YP_009068284.1, NC_025139.1
YP_009068507.1, NC_025141.1
YP_009070232.1, NC_025167.1
YP_009070558.1, NC_025175.1
YP_009071512.1, NC_025183.1
YP_190222.1, NC_006671.1

Genome annotation databases

GeneIDi10076131
10076142
13876868
13877052
13903673
13905334
13905363
13906404
13906709
13906924
13909533
13909568
14612524
17824300
17824435
18157686
20466965
20466993
20467118
20468340
20471961
20491414
20491639
20492529
20492626
20493584
2716540
3244915
35548256
3722457
4924718
5961992
6276043
8319064
8319163
9537966
9538101
9846067
KEGGiag:AAB59737
ag:CAA41038
ag:CAA45828
ag:CAA46344
ag:CAA46345
ag:CAA46346

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01749 Genomic DNA Translation: AAB59737.1
V00613 Genomic DNA Translation: CAA23886.1
X64523 Genomic DNA Translation: CAA45828.1
X57972 Genomic DNA Translation: CAA41038.1
X65252 Genomic DNA Translation: CAA46344.1
X65253 Genomic DNA Translation: CAA46345.1
X65254 Genomic DNA Translation: CAA46346.1
U89928 Genomic DNA Translation: AAB64386.1
U66885 Genomic DNA Translation: AAC48875.1
PIRiA93821 PNECP
S30113
RefSeqiNP_943295.1, NC_005248.1
NP_957565.1, NC_005327.1
WP_000027057.1, NZ_UIMY01000047.1
WP_063864949.1, NZ_BGKM01000047.1
WP_063865014.1, NZ_BGLS01000178.1
YP_001096393.1, NC_009132.1
YP_001693174.1, NC_010378.1
YP_001816609.1, NC_010558.1
YP_003108102.1, NC_013120.1
YP_003108210.1, NC_013121.1
YP_003829069.1, NC_014383.1
YP_003829170.1, NC_014384.1
YP_003937675.1, NC_014615.1
YP_004119720.1, NC_014843.1
YP_004119734.1, NC_014843.1
YP_006903139.1, NC_019047.1
YP_006939984.1, NC_018994.1
YP_006940092.1, NC_018995.1
YP_006952181.1, NC_019056.1
YP_006952421.1, NC_019062.1
YP_006952427.1, NC_019063.1
YP_006953479.1, NC_019073.1
YP_006953762.1, NC_019088.1
YP_006953988.1, NC_019091.1
YP_006954235.1, NC_019095.1
YP_007447512.1, NC_020278.2
YP_008864019.1, NC_022992.1
YP_008864686.1, NC_022996.1
YP_008995211.1, NC_023277.2
YP_009060387.1, NC_024960.1
YP_009060444.1, NC_024961.1
YP_009060580.1, NC_024967.1
YP_009061316.1, NC_024977.1
YP_009066523.1, NC_025106.1
YP_009068284.1, NC_025139.1
YP_009068507.1, NC_025141.1
YP_009070232.1, NC_025167.1
YP_009070558.1, NC_025175.1
YP_009071512.1, NC_025183.1
YP_190222.1, NC_006671.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AXBX-ray2.00A24-286[»]
1BT5X-ray1.80A24-286[»]
1BTLX-ray1.80A24-286[»]
1CK3X-ray2.28A24-284[»]
1ERMX-ray1.70A24-286[»]
1EROX-ray2.10A24-286[»]
1ERQX-ray1.90A24-286[»]
1ESUX-ray2.00A24-284[»]
1FQGX-ray1.70A24-286[»]
1JTDX-ray2.30A24-286[»]
1JTGX-ray1.73A/C24-286[»]
1JVJX-ray1.73A24-286[»]
1JWPX-ray1.75A24-286[»]
1JWVX-ray1.85A24-286[»]
1JWZX-ray1.80A24-286[»]
1LHYX-ray2.00A24-284[»]
1LI0X-ray1.61A24-284[»]
1LI9X-ray1.52A24-284[»]
1M40X-ray0.85A24-286[»]
1NXYX-ray1.60A24-286[»]
1NY0X-ray1.75A24-286[»]
1NYMX-ray1.20A24-286[»]
1NYYX-ray1.90A24-286[»]
1PZOX-ray1.90A24-284[»]
1PZPX-ray1.45A24-284[»]
1S0WX-ray2.30A/B24-286[»]
1TEMX-ray1.95A24-286[»]
1XPBX-ray1.90A24-286[»]
1XXMX-ray1.90A/B24-286[»]
1YT4X-ray1.40A24-284[»]
1ZG4X-ray1.55A1-284[»]
1ZG6X-ray2.10A1-284[»]
2B5RX-ray1.65A/B24-286[»]
2V1ZX-ray1.60A25-38[»]
A41-286[»]
2V20X-ray1.67A25-38[»]
A41-286[»]
3C7UX-ray2.20A/C24-286[»]
3C7VX-ray2.07A/C24-286[»]
3CMZX-ray1.92A24-286[»]
3DTMX-ray2.00A24-286[»]
3JYIX-ray2.70A/B/C/D/E/F24-286[»]
3TOIX-ray1.90A/B39-283[»]
4DXBX-ray2.29A/B24-226[»]
4DXCX-ray2.30A24-226[»]
4GKUX-ray1.92A24-286[»]
4IBRX-ray2.20A24-286[»]
4IBXX-ray2.68A/B/C/D/E24-286[»]
4ID4X-ray1.05A24-147[»]
A189-286[»]
4MEZX-ray2.05A/B24-286[»]
4QY5X-ray1.50A24-147[»]
A189-286[»]
4QY6X-ray1.15A24-147[»]
A189-286[»]
4R4RX-ray1.20A24-147[»]
A189-286[»]
4R4SX-ray1.10A24-149[»]
A189-286[»]
4RVAX-ray1.44A24-286[»]
4RX2X-ray2.31A/B/C/D/E/F/G/H24-286[»]
4RX3X-ray1.39A24-286[»]
4ZJ1X-ray1.54A1-286[»]
4ZJ2X-ray1.80A1-286[»]
4ZJ3X-ray1.70A1-286[»]
5HVIX-ray1.64A/B/C/D24-286[»]
5HW1X-ray1.70A/B/C/D24-286[»]
5HW5X-ray1.41A/B/C/D24-286[»]
5I52X-ray1.75A/B/C/D24-286[»]
5I63X-ray1.95A/B/C/D24-286[»]
5IQ8X-ray2.06A/B/C/D24-286[»]
5KKFX-ray1.82A/B/C/D24-286[»]
5KPUX-ray1.50A/B/C/D24-286[»]
5NPOX-ray1.95A24-286[»]
6APAX-ray1.86A/B/C/D24-286[»]
6AYKX-ray1.44A/B/C/D24-286[»]
6B2NX-ray2.00A/B/C/D24-286[»]
ProteinModelPortaliP62593
SMRiP62593
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP62593, 1 interactor

Chemistry databases

BindingDBiP62593
ChEMBLiCHEMBL2065
DrugBankiDB08551 (1R)-1-(2-THIENYLACETYLAMINO)-1-(3-CARBOXYPHENYL)METHYLBORONIC ACID
DB07466 (1R)-2-PHENYLACETAMIDO-2-(3-CARBOXYPHENYL)ETHYL BORONIC ACID
DB07464 1(R)-1-ACETAMIDO-2-(3-CARBOXY-2-HYDROXYPHENYL)ETHYL BORONIC ACID
DB02614 1(R)-1-Acetamido-2-(3-Carboxyphenyl)Ethyl Boronic Acid
DB04430 3-(4-Phenylamino-Phenylamino)-2-(1h-Tetrazol-5-Yl)-Acrylonitrile
DB07599 [(2-AMINO-ALPHA-METHOXYIMINO-4-THIAZOLYLACETYL)AMINO]METHYLBORONIC ACID
DB02841 [(2-Ethoxy-1-Naphthoyl)Amino]Methylboronic Acid
DB02642 [[N-(Benzyloxycarbonyl)Amino]Methyl]Phosphate
DB09060 Avibactam
DB01053 Benzylpenicillin
DB04035 Pinacol[[2-Amino-Alpha-(1-Carboxy-1-Methylethoxyimino)-4-Thiazoleacetyl]Amino]Methaneboronate

Proteomic databases

PRIDEiP62593

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi10076131
10076142
13876868
13877052
13903673
13905334
13905363
13906404
13906709
13906924
13909533
13909568
14612524
17824300
17824435
18157686
20466965
20466993
20467118
20468340
20471961
20491414
20491639
20492529
20492626
20493584
2716540
3244915
35548256
3722457
4924718
5961992
6276043
8319064
8319163
9537966
9538101
9846067
KEGGiag:AAB59737
ag:CAA41038
ag:CAA45828
ag:CAA46344
ag:CAA46345
ag:CAA46346

Phylogenomic databases

KOiK18698

Enzyme and pathway databases

BRENDAi3.5.2.6 2026
SABIO-RKiP62593

Miscellaneous databases

EvolutionaryTraceiP62593
PROiPR:P62593

Family and domain databases

InterProiView protein in InterPro
IPR012338 Beta-lactam/transpept-like
IPR000871 Beta-lactam_class-A
IPR023650 Beta-lactam_class-A_AS
PRINTSiPR00118 BLACTAMASEA
SUPFAMiSSF56601 SSF56601, 1 hit
PROSITEiView protein in PROSITE
PS00146 BETA_LACTAMASE_A, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBLAT_ECOLX
AccessioniPrimary (citable) accession number: P62593
Secondary accession number(s): P00810, Q47313
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 7, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid, Transposable element

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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