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Protein

Eukaryotic peptide chain release factor subunit 1

Gene

ETF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.1 Publication

GO - Molecular functioni

  • ribosome binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • sequence-specific mRNA binding Source: CAFA
  • translation release factor activity Source: UniProtKB
  • translation termination factor activity Source: ProtInc

GO - Biological processi

Keywordsi

Biological processNonsense-mediated mRNA decay, Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-HSA-72764 Eukaryotic Translation Termination
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic peptide chain release factor subunit 1
Short name:
Eukaryotic release factor 1
Short name:
eRF1
Alternative name(s):
Protein Cl1
TB3-1
Gene namesi
Name:ETF1
Synonyms:ERF1, RF1, SUP45L1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000120705.12
HGNCiHGNC:3477 ETF1
MIMi600285 gene
neXtProtiNX_P62495

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi185Q → R, I or N: Abolishes methylation by N6AMT1. 2 Publications1

Organism-specific databases

DisGeNETi2107
OpenTargetsiENSG00000120705
PharmGKBiPA27893

Polymorphism and mutation databases

BioMutaiETF1
DMDMi50402099

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001431382 – 437Eukaryotic peptide chain release factor subunit 1Add BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Cross-linki87Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei185N5-methylglutamine3 Publications1
Modified residuei347PhosphothreonineCombined sources1
Cross-linki404Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Methylated at Gln-185 by N6AMT1.3 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP62495
MaxQBiP62495
PaxDbiP62495
PeptideAtlasiP62495
PRIDEiP62495
ProteomicsDBi57403

PTM databases

iPTMnetiP62495
PhosphoSitePlusiP62495
SwissPalmiP62495

Expressioni

Gene expression databases

BgeeiENSG00000120705 Expressed in 236 organ(s), highest expression level in layer of synovial tissue
CleanExiHS_ETF1
ExpressionAtlasiP62495 baseline and differential
GenevisibleiP62495 HS

Organism-specific databases

HPAiCAB011686
HPA037511

Interactioni

Subunit structurei

Heterodimer of two subunits, one of which binds GTP. Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi108408, 55 interactors
ComplexPortaliCPX-2721 Translation release factor ERF1-ERF3 complex
CORUMiP62495
IntActiP62495, 28 interactors
MINTiP62495
STRINGi9606.ENSP00000353741

Structurei

Secondary structure

1437
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00310
ProteinModelPortaliP62495
SMRiP62495
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62495

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic release factor 1 family.Curated

Phylogenomic databases

eggNOGiKOG0688 Eukaryota
COG1503 LUCA
GeneTreeiENSGT00390000009004
HOGENOMiHOG000224681
HOVERGENiHBG005602
InParanoidiP62495
KOiK03265
OMAiGPGTEKM
OrthoDBiEOG091G0MEJ
PhylomeDBiP62495
TreeFamiTF105672

Family and domain databases

Gene3Di3.30.1330.30, 1 hit
3.30.960.10, 1 hit
InterProiView protein in InterPro
IPR005140 eRF1_1_Pelota
IPR005141 eRF1_2
IPR005142 eRF1_3
IPR029064 L30e-like
IPR004403 Peptide_chain-rel_eRF1/aRF1
IPR024049 Release_factor_eRF1/aRF1_N
PANTHERiPTHR10113 PTHR10113, 1 hit
PfamiView protein in Pfam
PF03463 eRF1_1, 1 hit
PF03464 eRF1_2, 1 hit
PF03465 eRF1_3, 1 hit
SMARTiView protein in SMART
SM01194 eRF1_1, 1 hit
SUPFAMiSSF55315 SSF55315, 1 hit
SSF55481 SSF55481, 1 hit
TIGRFAMsiTIGR03676 aRF1/eRF1, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P62495-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MADDPSAADR NVEIWKIKKL IKSLEAARGN GTSMISLIIP PKDQISRVAK
60 70 80 90 100
MLADEFGTAS NIKSRVNRLS VLGAITSVQQ RLKLYNKVPP NGLVVYCGTI
110 120 130 140 150
VTEEGKEKKV NIDFEPFKPI NTSLYLCDNK FHTEALTALL SDDSKFGFIV
160 170 180 190 200
IDGSGALFGT LQGNTREVLH KFTVDLPKKH GRGGQSALRF ARLRMEKRHN
210 220 230 240 250
YVRKVAETAV QLFISGDKVN VAGLVLAGSA DFKTELSQSD MFDQRLQSKV
260 270 280 290 300
LKLVDISYGG ENGFNQAIEL STEVLSNVKF IQEKKLIGRY FDEISQDTGK
310 320 330 340 350
YCFGVEDTLK ALEMGAVEIL IVYENLDIMR YVLHCQGTEE EKILYLTPEQ
360 370 380 390 400
EKDKSHFTDK ETGQEHELIE SMPLLEWFAN NYKKFGATLE IVTDKSQEGS
410 420 430
QFVKGFGGIG GILRYRVDFQ GMEYQGGDDE FFDLDDY
Length:437
Mass (Da):49,031
Last modified:January 23, 2007 - v3
Checksum:iCECC50D100E59D19
GO
Isoform 2 (identifier: P62495-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.

Show »
Length:404
Mass (Da):45,463
Checksum:iF70FB4B7D81FD5C1
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7Z7P8B7Z7P8_HUMAN
Eukaryotic peptide chain release fa...
ETF1
423Annotation score:
I3L492I3L492_HUMAN
Eukaryotic peptide chain release fa...
ETF1
208Annotation score:
D6RCB3D6RCB3_HUMAN
Eukaryotic peptide chain release fa...
ETF1
95Annotation score:
D6RJE8D6RJE8_HUMAN
Eukaryotic peptide chain release fa...
ETF1
119Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0561891 – 33Missing in isoform 2. 2 PublicationsAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75715 mRNA Translation: AAA36665.1 Sequence problems.
X81625 mRNA Translation: CAA57281.1
U90176 mRNA Translation: AAB49726.1
AF095901 Genomic DNA Translation: AAD43966.1
BT007374 mRNA Translation: AAP36038.1
AK312510 mRNA Translation: BAG35411.1
AC011385 Genomic DNA No translation available.
AC113403 Genomic DNA No translation available.
CH471062 Genomic DNA Translation: EAW62130.1
CH471062 Genomic DNA Translation: EAW62131.1
CH471062 Genomic DNA Translation: EAW62132.1
BC088358 mRNA Translation: AAH88358.1
BC014269 mRNA Translation: AAH14269.1
CCDSiCCDS4207.1 [P62495-1]
CCDS75313.1 [P62495-2]
PIRiS50853
RefSeqiNP_001243231.1, NM_001256302.1 [P62495-2]
NP_001278903.1, NM_001291974.1 [P62495-2]
NP_001278904.1, NM_001291975.1 [P62495-2]
NP_004721.1, NM_004730.3 [P62495-1]
XP_005271978.1, XM_005271921.1 [P62495-2]
UniGeneiHs.483494

Genome annotation databases

EnsembliENST00000360541; ENSP00000353741; ENSG00000120705 [P62495-1]
ENST00000499810; ENSP00000421288; ENSG00000120705 [P62495-2]
GeneIDi2107
KEGGihsa:2107
UCSCiuc003ldc.6 human [P62495-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75715 mRNA Translation: AAA36665.1 Sequence problems.
X81625 mRNA Translation: CAA57281.1
U90176 mRNA Translation: AAB49726.1
AF095901 Genomic DNA Translation: AAD43966.1
BT007374 mRNA Translation: AAP36038.1
AK312510 mRNA Translation: BAG35411.1
AC011385 Genomic DNA No translation available.
AC113403 Genomic DNA No translation available.
CH471062 Genomic DNA Translation: EAW62130.1
CH471062 Genomic DNA Translation: EAW62131.1
CH471062 Genomic DNA Translation: EAW62132.1
BC088358 mRNA Translation: AAH88358.1
BC014269 mRNA Translation: AAH14269.1
CCDSiCCDS4207.1 [P62495-1]
CCDS75313.1 [P62495-2]
PIRiS50853
RefSeqiNP_001243231.1, NM_001256302.1 [P62495-2]
NP_001278903.1, NM_001291974.1 [P62495-2]
NP_001278904.1, NM_001291975.1 [P62495-2]
NP_004721.1, NM_004730.3 [P62495-1]
XP_005271978.1, XM_005271921.1 [P62495-2]
UniGeneiHs.483494

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DT9X-ray2.70A1-437[»]
2HSTNMR-A140-275[»]
2KTUNMR-A276-437[»]
2KTVNMR-A276-437[»]
2LGTNMR-A1-142[»]
2LLXNMR-A1-142[»]
2MQ6NMR-A1-142[»]
2MQ9NMR-A1-142[»]
3E1YX-ray3.80A/B/C/D1-437[»]
3J5Yelectron microscopy9.70A7-420[»]
3JAGelectron microscopy3.65ii6-421[»]
3JAHelectron microscopy3.45ii6-421[»]
3JAIelectron microscopy3.65ii6-421[»]
4D5Nelectron microscopy9.00A5-437[»]
4D61electron microscopy9.00h5-437[»]
5A8Lelectron microscopy3.80Q7-437[»]
5LZTelectron microscopy3.65ii1-437[»]
5LZUelectron microscopy3.75ii1-437[»]
5LZVelectron microscopy3.35ii1-437[»]
6D90electron microscopy3.20jj1-437[»]
DisProtiDP00310
ProteinModelPortaliP62495
SMRiP62495
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108408, 55 interactors
ComplexPortaliCPX-2721 Translation release factor ERF1-ERF3 complex
CORUMiP62495
IntActiP62495, 28 interactors
MINTiP62495
STRINGi9606.ENSP00000353741

PTM databases

iPTMnetiP62495
PhosphoSitePlusiP62495
SwissPalmiP62495

Polymorphism and mutation databases

BioMutaiETF1
DMDMi50402099

Proteomic databases

EPDiP62495
MaxQBiP62495
PaxDbiP62495
PeptideAtlasiP62495
PRIDEiP62495
ProteomicsDBi57403

Protocols and materials databases

DNASUi2107
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360541; ENSP00000353741; ENSG00000120705 [P62495-1]
ENST00000499810; ENSP00000421288; ENSG00000120705 [P62495-2]
GeneIDi2107
KEGGihsa:2107
UCSCiuc003ldc.6 human [P62495-1]

Organism-specific databases

CTDi2107
DisGeNETi2107
EuPathDBiHostDB:ENSG00000120705.12
GeneCardsiETF1
HGNCiHGNC:3477 ETF1
HPAiCAB011686
HPA037511
MIMi600285 gene
neXtProtiNX_P62495
OpenTargetsiENSG00000120705
PharmGKBiPA27893
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0688 Eukaryota
COG1503 LUCA
GeneTreeiENSGT00390000009004
HOGENOMiHOG000224681
HOVERGENiHBG005602
InParanoidiP62495
KOiK03265
OMAiGPGTEKM
OrthoDBiEOG091G0MEJ
PhylomeDBiP62495
TreeFamiTF105672

Enzyme and pathway databases

ReactomeiR-HSA-72764 Eukaryotic Translation Termination
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Miscellaneous databases

ChiTaRSiETF1 human
EvolutionaryTraceiP62495
GeneWikiiEukaryotic_release_factors
GenomeRNAii2107
PROiPR:P62495
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120705 Expressed in 236 organ(s), highest expression level in layer of synovial tissue
CleanExiHS_ETF1
ExpressionAtlasiP62495 baseline and differential
GenevisibleiP62495 HS

Family and domain databases

Gene3Di3.30.1330.30, 1 hit
3.30.960.10, 1 hit
InterProiView protein in InterPro
IPR005140 eRF1_1_Pelota
IPR005141 eRF1_2
IPR005142 eRF1_3
IPR029064 L30e-like
IPR004403 Peptide_chain-rel_eRF1/aRF1
IPR024049 Release_factor_eRF1/aRF1_N
PANTHERiPTHR10113 PTHR10113, 1 hit
PfamiView protein in Pfam
PF03463 eRF1_1, 1 hit
PF03464 eRF1_2, 1 hit
PF03465 eRF1_3, 1 hit
SMARTiView protein in SMART
SM01194 eRF1_1, 1 hit
SUPFAMiSSF55315 SSF55315, 1 hit
SSF55481 SSF55481, 1 hit
TIGRFAMsiTIGR03676 aRF1/eRF1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiERF1_HUMAN
AccessioniPrimary (citable) accession number: P62495
Secondary accession number(s): B2R6B4
, D3DQC1, P46055, Q5M7Z7, Q96CG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 23, 2007
Last modified: October 10, 2018
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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