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Protein

Abl interactor 2

Gene

Abi2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation (By similarity). Acts as regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge (By similarity). Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain (PubMed:15572692). In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth (PubMed:27605705). Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface (PubMed:15572692). Also required for nascent adherens junction assembly in epithelial cells (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processNeurogenesis

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs

Names & Taxonomyi

Protein namesi
Recommended name:
Abl interactor 21 Publication
Alternative name(s):
Abelson interactor 2By similarity
Short name:
Abi-2By similarity
Abl-binding protein 3
Short name:
AblBP3
Arg-binding protein 1By similarity
Short name:
ArgBP1By similarity
Gene namesi
Name:Abi21 PublicationImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:106913 Abi2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Knockout mice are born at the expected Mendelian rate. Mutant mice have grossly distorted lenses due to defective orientation and migration of secondary lens fibers associated with impaired anterior and posterior sutures formation. Mice exhibit neuroanatomical abnormalities characterized by defective dendritic spine morphology and misoriented cortical and hippocampal neurons, causing profound deficits in learning and memory.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001917911 – 446Abl interactor 2Add BLAST446

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei40PhosphoserineCombined sources1
Modified residuei177PhosphoserineBy similarity1
Modified residuei221PhosphoserineBy similarity1
Modified residuei294PhosphothreonineCombined sources1
Modified residuei301PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by ABL1.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP62484
MaxQBiP62484
PaxDbiP62484
PeptideAtlasiP62484
PRIDEiP62484

PTM databases

iPTMnetiP62484
PhosphoSitePlusiP62484

Expressioni

Tissue specificityi

Expresses in embryonic and adult brain. In adult brain prominently expressed in the neocortex, hippocampus and dentate gyrus.1 Publication

Developmental stagei

Detected at E10 in developing brain, and expression is more prominent in the neuroepithelium compared to the surrounding tissue. At E12 expression is enhanced throughout the CNS and is detected along the full length of the spinal chord. At E16 expression remains enhanced in the CNS, and is particularly prominent in the olfactory bulb (PubMed:10995551). Also highly expressed in dorsal root ganglia (PubMed:10995551). At E18.5 is prominently expressed in the marginal zone of the cortex, an area rich in neuronal and glial cell projections (at protein level) (PubMed:15572692). Detected at E16.5 in ocular lens, specifically localized where the tips of migrating secondary fibers forms adherens junctions with anterior epithelial cells (at protein level) (PubMed:15572692).2 Publications

Gene expression databases

BgeeiENSMUSG00000026782 Expressed in 238 organ(s), highest expression level in lateral septal nucleus
CleanExiMM_ABI2
ExpressionAtlasiP62484 baseline and differential
GenevisibleiP62484 MM

Interactioni

Subunit structurei

Component of the WAVE complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WASF1/WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1 (By similarity). Interacts (via SH3 domain) with ABL1 and ABL2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi236719, 4 interactors
DIPiDIP-48418N
IntActiP62484, 5 interactors
STRINGi10090.ENSMUSP00000058754

Structurei

3D structure databases

ProteinModelPortaliP62484
SMRiP62484
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 107t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63
Domaini384 – 443SH3PROSITE-ProRule annotationAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi166 – 356Pro-richAdd BLAST191

Domaini

The SH3 domain is critical for binding to ABL1 and ABL2.By similarity

Sequence similaritiesi

Belongs to the ABI family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG2546 Eukaryota
ENOG410Y0MH LUCA
GeneTreeiENSGT00390000003756
HOGENOMiHOG000293213
HOVERGENiHBG050446
InParanoidiP62484
KOiK05751
PhylomeDBiP62484
TreeFamiTF314303

Family and domain databases

CDDicd11972 SH3_Abi2, 1 hit
InterProiView protein in InterPro
IPR028457 ABI
IPR036993 ABI2
IPR035726 Abi2_SH3
IPR012849 Abl-interactor_HHR_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR000727 T_SNARE_dom
PANTHERiPTHR10460 PTHR10460, 1 hit
PTHR10460:SF0 PTHR10460:SF0, 1 hit
PfamiView protein in Pfam
PF07815 Abi_HHR, 1 hit
PF14604 SH3_9, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit
PS50192 T_SNARE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All

P62484-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAELQMLLEE EIPGGRRALF DSYTNLERVA DYCENNYIQS PDKQRALEET
60 70 80 90 100
KAYTTQSLAS VAYLINTLAN NVLQMLDIQA SQLRRMESSI NHISQTVDIH
110 120 130 140 150
KEKVARREIG ILTTNKNTSR THKIIAPANL ERPVRYIRKP IDYTILDDIG
160 170 180 190 200
HGVKVSTQNM KMGGLPRTTP PTQKPPSPPM SGKGTLGRHS PYRTLEPVRP
210 220 230 240 250
PVVPNDYVPS PTRNMAPSQQ SPVRTASVNQ RNRTYSSSGS SGGSHPSSRS
260 270 280 290 300
SSRENSGSGS VGVPIAVPTP SPPSVFPGHP VQFYSMNRPA SRHTPPTIGG
310 320 330 340 350
SLPYRRPPSI TSQTSLQNQM NGGPFYNQNP VSDTPPPPPP VEEPVFDESP
360 370 380 390 400
PPPPPPEDYE EEEAAVVEYS DPYAEEDPPW APRAYLEKVV AIYDYTKDKE
410 420 430 440
DELSFQEGAI IYVIKKNDDG WYEGVMNGVT GLFPGNYVES IMHYSE
Length:446
Mass (Da):49,387
Last modified:July 19, 2004 - v1
Checksum:i8EC1FB543D93BEAA
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WPP8A0A087WPP8_MOUSE
Abl interactor 2
Abi2
475Annotation score:
A0A087WR90A0A087WR90_MOUSE
Abl interactor 2
Abi2
151Annotation score:
A0A087WRS3A0A087WRS3_MOUSE
Abl interactor 2
Abi2
458Annotation score:
Q6AXH6Q6AXH6_MOUSE
Abi2 protein
Abi2
480Annotation score:
Q6AXD2Q6AXD2_MOUSE
Abi2 protein
Abi2
487Annotation score:
A0A087WNT3A0A087WNT3_MOUSE
Abl interactor 2
Abi2
360Annotation score:
A0A087WP64A0A087WP64_MOUSE
Abl interactor 2
Abi2
252Annotation score:
A0A087WNU9A0A087WNU9_MOUSE
Abl interactor 2
Abi2
395Annotation score:
A0A087WPE6A0A087WPE6_MOUSE
Abl interactor 2
Abi2
337Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056345 mRNA Translation: AAH56345.1
CCDSiCCDS14991.1
RefSeqiNP_001185499.1, NM_001198570.1
NP_001185500.1, NM_001198571.1
NP_937760.1, NM_198127.2
UniGeneiMm.212066
Mm.465571

Genome annotation databases

EnsembliENSMUST00000052332; ENSMUSP00000058754; ENSMUSG00000026782
GeneIDi329165
KEGGimmu:329165
UCSCiuc007beq.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056345 mRNA Translation: AAH56345.1
CCDSiCCDS14991.1
RefSeqiNP_001185499.1, NM_001198570.1
NP_001185500.1, NM_001198571.1
NP_937760.1, NM_198127.2
UniGeneiMm.212066
Mm.465571

3D structure databases

ProteinModelPortaliP62484
SMRiP62484
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi236719, 4 interactors
DIPiDIP-48418N
IntActiP62484, 5 interactors
STRINGi10090.ENSMUSP00000058754

PTM databases

iPTMnetiP62484
PhosphoSitePlusiP62484

Proteomic databases

EPDiP62484
MaxQBiP62484
PaxDbiP62484
PeptideAtlasiP62484
PRIDEiP62484

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052332; ENSMUSP00000058754; ENSMUSG00000026782
GeneIDi329165
KEGGimmu:329165
UCSCiuc007beq.2 mouse

Organism-specific databases

CTDi10152
MGIiMGI:106913 Abi2

Phylogenomic databases

eggNOGiKOG2546 Eukaryota
ENOG410Y0MH LUCA
GeneTreeiENSGT00390000003756
HOGENOMiHOG000293213
HOVERGENiHBG050446
InParanoidiP62484
KOiK05751
PhylomeDBiP62484
TreeFamiTF314303

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs

Miscellaneous databases

PROiPR:P62484
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026782 Expressed in 238 organ(s), highest expression level in lateral septal nucleus
CleanExiMM_ABI2
ExpressionAtlasiP62484 baseline and differential
GenevisibleiP62484 MM

Family and domain databases

CDDicd11972 SH3_Abi2, 1 hit
InterProiView protein in InterPro
IPR028457 ABI
IPR036993 ABI2
IPR035726 Abi2_SH3
IPR012849 Abl-interactor_HHR_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR000727 T_SNARE_dom
PANTHERiPTHR10460 PTHR10460, 1 hit
PTHR10460:SF0 PTHR10460:SF0, 1 hit
PfamiView protein in Pfam
PF07815 Abi_HHR, 1 hit
PF14604 SH3_9, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit
PS50192 T_SNARE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiABI2_MOUSE
AccessioniPrimary (citable) accession number: P62484
Secondary accession number(s): Q6PHU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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