UniProtKB - P62483 (KCAB2_RAT)
Voltage-gated potassium channel subunit beta-2
Kcnab2
Functioni
Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits (PubMed:9763623, PubMed:21357749).
Contributes to the regulation of nerve signaling, and prevents neuronal hyperexcitability (By similarity).
Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (PubMed:10896669, PubMed:16770729, PubMed:18003609, PubMed:21357749).
Promotes potassium channel closure via a mechanism that does not involve physical obstruction of the channel pore (PubMed:21357749).
Modulates the functional properties of KCNA4 (PubMed:9763623).
Modulates the functional properties of KCNA5 (By similarity).
Enhances KCNB2 channel activity (By similarity).
Binds NADPH and has NADPH-dependent aldoketoreductase activity (PubMed:18672894, PubMed:21209188).
Has broad substrate specificity and can catalyze the reduction of methylglyoxal, 9,10-phenanthrenequinone, prostaglandin J2, 4-nitrobenzaldehyde, 4-nitroacetophenone and 4-oxo-trans-2-nonenal (in vitro) (PubMed:18672894).
By similarity6 PublicationspH dependencei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 63 | NADPCombined sources1 Publication | 1 | |
Binding sitei | 85 | NADPCombined sources1 Publication | 1 | |
Active sitei | 90 | Proton donor/acceptor1 Publication | 1 | |
Binding sitei | 90 | NADP1 Publication | 1 | |
Binding sitei | 214 | NADPCombined sources1 Publication | 1 | |
Binding sitei | 254 | NADPCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 56 – 57 | NADPCombined sources1 Publication | 2 | |
Nucleotide bindingi | 188 – 189 | NADPCombined sources1 Publication | 2 | |
Nucleotide bindingi | 243 – 248 | NADPCombined sources1 Publication | 6 | |
Nucleotide bindingi | 262 – 264 | NADPCombined sources | 3 | |
Nucleotide bindingi | 323 – 333 | NADPCombined sources1 PublicationAdd BLAST | 11 |
GO - Molecular functioni
- aldo-keto reductase (NADP) activity Source: UniProtKB
- D-threo-aldose 1-dehydrogenase activity Source: InterPro
- potassium channel regulator activity Source: UniProtKB
- protein-containing complex binding Source: RGD
- transmembrane transporter binding Source: RGD
- voltage-gated potassium channel activity Source: InterPro
GO - Biological processi
- hematopoietic progenitor cell differentiation Source: RGD
- myoblast differentiation Source: RGD
- NADPH oxidation Source: UniProtKB
- neuromuscular process Source: RGD
- regulation of action potential Source: GO_Central
- regulation of potassium ion transmembrane transport Source: UniProtKB
- regulation of protein localization to cell surface Source: UniProtKB
Keywordsi
Molecular function | Ion channel, Oxidoreductase, Voltage-gated channel |
Biological process | Ion transport, Potassium transport, Transport |
Ligand | NADP, Potassium |
Enzyme and pathway databases
Reactomei | R-RNO-1296072, Voltage gated Potassium channels R-RNO-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Voltage-gated potassium channel subunit beta-2 (EC:1.1.1.-2 Publications)Alternative name(s): K(+) channel subunit beta-2 Kv-beta-2 |
Gene namesi | Name:Kcnab2 Synonyms:Ckbeta2, Kcnb3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61828, Kcnab2 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Plasma membrane
- Cell membrane 1 Publication; Peripheral membrane protein Curated; Cytoplasmic side Curated
Cytoskeleton
- cytoskeleton 1 Publication
Other locations
- Membrane 2 Publications; Peripheral membrane protein Curated; Cytoplasmic side Curated
- axon 2 Publications
- synaptosome 1 Publication
Note: Recruited to the cytoplasmic side of the cell membrane via its interaction with pore-forming potassium channel alpha subunits. Associates with microtubules when unphosphorylated (PubMed:21357749).Curated1 Publication
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Cytosol
- cytosol Source: UniProtKB
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
- voltage-gated potassium channel complex Source: RGD
Other locations
- axon Source: UniProtKB
- axon terminus Source: RGD
- juxtaparanode region of axon Source: UniProtKB
- membrane Source: RGD
- neuron projection Source: UniProtKB
- pinceau fiber Source: UniProtKB
- postsynaptic density Source: RGD
- potassium channel complex Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Synapse, SynaptosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 9 | S → A: Impairs interaction with MAPRE1 and association with microtubules. 1 Publication | 1 | |
Mutagenesisi | 20 | S → A: No effect on interaction with MAPRE1 and association with microtubules. 1 Publication | 1 | |
Mutagenesisi | 31 | S → A: Impairs interaction with MAPRE1 and association with microtubules. 1 Publication | 1 | |
Mutagenesisi | 90 | Y → F: Abolishes enzyme activity, but has no effect on NADPH binding. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000148748 | 1 – 367 | Voltage-gated potassium channel subunit beta-2Add BLAST | 367 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 9 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 14 | PhosphoserineBy similarity | 1 | |
Modified residuei | 20 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 28 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 28 | Omega-N-methylarginine; alternateBy similarity | 1 | |
Modified residuei | 31 | PhosphoserineBy similarity | 1 | |
Modified residuei | 112 | Phosphoserine1 Publication | 1 | |
Modified residuei | 124 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
PaxDbi | P62483 |
PTM databases
iPTMneti | P62483 |
PhosphoSitePlusi | P62483 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000011550, Expressed in frontal cortex and 22 other tissues |
ExpressionAtlasi | P62483, baseline and differential |
Genevisiblei | P62483, RN |
Interactioni
Subunit structurei
Homotetramer (PubMed:10884227, PubMed:16002581, PubMed:18004376, PubMed:18806782, PubMed:20534430, PubMed:20360102, PubMed:23705070). Interaction with tetrameric potassium channel alpha subunits gives rise to a heterooctamer (PubMed:10884227, PubMed:16002581, PubMed:18004376, PubMed:18806782, PubMed:20534430, PubMed:20360102, PubMed:23705070). Identified in potassium channel complexes containing KCNA1, KCNA2, KCNA4, KCNA5, KCNA6, KCNAB1 and KCNAB2 (PubMed:9334400).
Interacts with KCNA1 (PubMed:23318870, PubMed:10884227).
Interacts with KCNA2 (PubMed:18003609, PubMed:21357749, PubMed:23318870, PubMed:16002581, PubMed:18004376, PubMed:18806782, PubMed:20534430, PubMed:20360102, PubMed:23705070).
Interacts with KCNA4 and KCND3 (PubMed:12860406).
Interacts with KCNA5 (By similarity).
Interacts with KCNB2 (By similarity).
Interacts (in unphosphorylated form) with MAPRE1 (PubMed:21357749).
Forms a ternary complex with SQSTM1 and PRKCZ (PubMed:10477520).
By similarity14 PublicationsGO - Molecular functioni
- transmembrane transporter binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248350, 7 interactors |
CORUMi | P62483 |
IntActi | P62483, 2 interactors |
MINTi | P62483 |
STRINGi | 10116.ENSRNOP00000015840 |
Structurei
Secondary structure
3D structure databases
SMRi | P62483 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P62483 |
Family & Domainsi
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1575, Eukaryota |
GeneTreei | ENSGT00940000157867 |
InParanoidi | P62483 |
OrthoDBi | 1106773at2759 |
PhylomeDBi | P62483 |
TreeFami | TF324563 |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR005983, K_chnl_volt-dep_bsu_KCNAB IPR005399, K_chnl_volt-dep_bsu_KCNAB-rel IPR005401, K_chnl_volt-dep_bsu_KCNAB2 IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
PANTHERi | PTHR43150, PTHR43150, 1 hit |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PRINTSi | PR01579, KCNAB2CHANEL PR01577, KCNABCHANNEL |
SUPFAMi | SSF51430, SSF51430, 1 hit |
TIGRFAMsi | TIGR01293, Kv_beta, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MYPESTTGSP ARLSLRQTGS PGMIYSTRYG SPKRQLQFYR NLGKSGLRVS
60 70 80 90 100
CLGLGTWVTF GGQITDEMAE HLMTLAYDNG INLFDTAEVY AAGKAEVVLG
110 120 130 140 150
NIIKKKGWRR SSLVITTKIF WGGKAETERG LSRKHIIEGL KASLERLQLE
160 170 180 190 200
YVDVVFANRP DPNTPMEETV RAMTHVINQG MAMYWGTSRW SSMEIMEAYS
210 220 230 240 250
VARQFNLIPP ICEQAEYHMF QREKVEVQLP ELFHKIGVGA MTWSPLACGI
260 270 280 290 300
VSGKYDSGIP PYSRASLKGY QWLKDKILSE EGRRQQAKLK ELQAIAERLG
310 320 330 340 350
CTLPQLAIAW CLRNEGVSSV LLGASNAEQL MENIGAIQVL PKLSSSIVHE
360
IDSILGNKPY SKKDYRS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K670 | A0A0G2K670_RAT | K(+) channel subunit beta-2 | Kcnab2 | 383 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76724 mRNA Translation: CAA54142.1 |
PIRi | S45312 |
RefSeqi | NP_059000.1, NM_017304.2 |
Genome annotation databases
Ensembli | ENSRNOT00000015840; ENSRNOP00000015840; ENSRNOG00000011550 |
GeneIDi | 29738 |
KEGGi | rno:29738 |
UCSCi | RGD:61828, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76724 mRNA Translation: CAA54142.1 |
PIRi | S45312 |
RefSeqi | NP_059000.1, NM_017304.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EXB | X-ray | 2.10 | A | 36-367 | [»] | |
1QRQ | X-ray | 2.80 | A/B/C/D | 36-360 | [»] | |
2A79 | X-ray | 2.90 | A | 36-367 | [»] | |
2R9R | X-ray | 2.40 | A/G | 36-367 | [»] | |
3EAU | X-ray | 1.82 | A | 36-361 | [»] | |
3EB3 | X-ray | 2.00 | A | 36-361 | [»] | |
3EB4 | X-ray | 2.00 | A | 36-361 | [»] | |
3LNM | X-ray | 2.90 | A/C | 36-367 | [»] | |
3LUT | X-ray | 2.90 | A | 1-367 | [»] | |
4JTA | X-ray | 2.50 | A/P | 36-367 | [»] | |
4JTC | X-ray | 2.56 | A/G | 36-367 | [»] | |
4JTD | X-ray | 2.54 | A/G | 36-367 | [»] | |
5WIE | X-ray | 3.30 | A/G | 35-367 | [»] | |
6CI1 | electron microscopy | 4.90 | A/B/C/D/E/F/G/H | 36-361 | [»] | |
6EBK | electron microscopy | 3.30 | A/C/E/G | 37-367 | [»] | |
6EBL | electron microscopy | 3.00 | A/C/E/G | 37-367 | [»] | |
SMRi | P62483 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 248350, 7 interactors |
CORUMi | P62483 |
IntActi | P62483, 2 interactors |
MINTi | P62483 |
STRINGi | 10116.ENSRNOP00000015840 |
PTM databases
iPTMneti | P62483 |
PhosphoSitePlusi | P62483 |
Proteomic databases
PaxDbi | P62483 |
Protocols and materials databases
ABCDi | P62483, 3 sequenced antibodies |
Genome annotation databases
Ensembli | ENSRNOT00000015840; ENSRNOP00000015840; ENSRNOG00000011550 |
GeneIDi | 29738 |
KEGGi | rno:29738 |
UCSCi | RGD:61828, rat |
Organism-specific databases
CTDi | 8514 |
RGDi | 61828, Kcnab2 |
Phylogenomic databases
eggNOGi | KOG1575, Eukaryota |
GeneTreei | ENSGT00940000157867 |
InParanoidi | P62483 |
OrthoDBi | 1106773at2759 |
PhylomeDBi | P62483 |
TreeFami | TF324563 |
Enzyme and pathway databases
Reactomei | R-RNO-1296072, Voltage gated Potassium channels R-RNO-6798695, Neutrophil degranulation |
Miscellaneous databases
EvolutionaryTracei | P62483 |
PROi | PR:P62483 |
Gene expression databases
Bgeei | ENSRNOG00000011550, Expressed in frontal cortex and 22 other tissues |
ExpressionAtlasi | P62483, baseline and differential |
Genevisiblei | P62483, RN |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR005983, K_chnl_volt-dep_bsu_KCNAB IPR005399, K_chnl_volt-dep_bsu_KCNAB-rel IPR005401, K_chnl_volt-dep_bsu_KCNAB2 IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
PANTHERi | PTHR43150, PTHR43150, 1 hit |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PRINTSi | PR01579, KCNAB2CHANEL PR01577, KCNABCHANNEL |
SUPFAMi | SSF51430, SSF51430, 1 hit |
TIGRFAMsi | TIGR01293, Kv_beta, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KCAB2_RAT | |
Accessioni | P62483Primary (citable) accession number: P62483 Secondary accession number(s): P97381, Q60942, Q64284 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2004 |
Last sequence update: | July 5, 2004 | |
Last modified: | February 23, 2022 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families