UniProtKB - P62450 (HIS6_DESVH)
Protein
Imidazole glycerol phosphate synthase subunit HisF
Gene
hisF
Organism
Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)
Status
Functioni
IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.UniRule annotation
Catalytic activityi
- 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H+ + L-glutamateUniRule annotationEC:4.3.2.10UniRule annotation
: L-histidine biosynthesis Pathwayi
This protein is involved in step 5 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotationProteins known to be involved in the 9 steps of the subpathway in this organism are:
- ATP phosphoribosyltransferase (hisG)
- no protein annotated in this organism
- Phosphoribosyl-AMP cyclohydrolase (hisI)
- 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
- Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
- Imidazoleglycerol-phosphate dehydratase (hisB)
- Histidinol-phosphate aminotransferase (hisC)
- Histidinol-phosphatase (DVU_2490)
- Histidinol dehydrogenase (hisD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 11 | UniRule annotation | 1 | |
Active sitei | 130 | UniRule annotation | 1 |
GO - Molecular functioni
- imidazoleglycerol-phosphate synthase activity Source: UniProtKB-UniRule
- lyase activity Source: UniProtKB-KW
GO - Biological processi
- histidine biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Histidine biosynthesis |
Enzyme and pathway databases
BioCyci | DVUL882:G1GT1-305-MONOMER |
UniPathwayi | UPA00031;UER00010 |
Names & Taxonomyi
Protein namesi | Recommended name: Imidazole glycerol phosphate synthase subunit HisFUniRule annotation (EC:4.3.2.10UniRule annotation)Alternative name(s): IGP synthase cyclase subunitUniRule annotation IGP synthase subunit HisFUniRule annotation ImGP synthase subunit HisFUniRule annotation Short name: IGPS subunit HisFUniRule annotation |
Gene namesi | Name:hisFUniRule annotation Ordered Locus Names:DVU_0286 |
Organismi | Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303) |
Taxonomic identifieri | 882 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000142154 | 1 – 259 | Imidazole glycerol phosphate synthase subunit HisFAdd BLAST | 259 |
Proteomic databases
PaxDbi | P62450 |
Interactioni
Subunit structurei
Heterodimer of HisH and HisF.
UniRule annotationBinary interactionsi
P62450
With | #Exp. | IntAct |
---|---|---|
hisH [P61779] | 3 | EBI-10064978,EBI-10064985 |
Protein-protein interaction databases
IntActi | P62450, 3 interactors |
STRINGi | 882.DVU_0286 |
Family & Domainsi
Sequence similaritiesi
Belongs to the HisA/HisF family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0107, Bacteria |
HOGENOMi | CLU_048577_4_0_7 |
OMAi | KRIVPCL |
PhylomeDBi | P62450 |
Family and domain databases
CDDi | cd04731, HisF, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01013, HisF, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006062, His_biosynth IPR004651, HisF IPR011060, RibuloseP-bd_barrel |
Pfami | View protein in Pfam PF00977, His_biosynth, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00735, hisF, 1 hit |
i Sequence
Sequence statusi: Complete.
P62450-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLSKRIIPCL DVRAGRLTKG VKFEGNVDIG DPVATARRYY EEGADEIVFY
60 70 80 90 100
DITASHEDRG IFLDVVERVA SEIFIPFSVG GGINTVDDMR AVLMAGAEKV
110 120 130 140 150
SVNSGAVKTP DIISQGAAAF GSQAIVVGMD VKQVEKSATI PSGYEIVIHG
160 170 180 190 200
GRKYMGMDAI EWAKTCESLG AGELCVNSID ADGTKDGYEL TLTRMISDAV
210 220 230 240 250
TIPVIASGGA GSPEHMYDAL TRGGASAALI ASIVHYGTYT IPDLKRRISG
MGAKMRMVW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017285 Genomic DNA Translation: AAS94769.1 |
RefSeqi | WP_010937595.1, NZ_CABHLV010000001.1 YP_009510.1, NC_002937.3 |
Genome annotation databases
EnsemblBacteriai | AAS94769; AAS94769; DVU_0286 |
KEGGi | dvu:DVU0286 |
PATRICi | fig|882.5.peg.273 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017285 Genomic DNA Translation: AAS94769.1 |
RefSeqi | WP_010937595.1, NZ_CABHLV010000001.1 YP_009510.1, NC_002937.3 |
3D structure databases
SMRi | P62450 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P62450, 3 interactors |
STRINGi | 882.DVU_0286 |
Proteomic databases
PaxDbi | P62450 |
Genome annotation databases
EnsemblBacteriai | AAS94769; AAS94769; DVU_0286 |
KEGGi | dvu:DVU0286 |
PATRICi | fig|882.5.peg.273 |
Phylogenomic databases
eggNOGi | COG0107, Bacteria |
HOGENOMi | CLU_048577_4_0_7 |
OMAi | KRIVPCL |
PhylomeDBi | P62450 |
Enzyme and pathway databases
UniPathwayi | UPA00031;UER00010 |
BioCyci | DVUL882:G1GT1-305-MONOMER |
Family and domain databases
CDDi | cd04731, HisF, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01013, HisF, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006062, His_biosynth IPR004651, HisF IPR011060, RibuloseP-bd_barrel |
Pfami | View protein in Pfam PF00977, His_biosynth, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00735, hisF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HIS6_DESVH | |
Accessioni | P62450Primary (citable) accession number: P62450 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2004 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 93 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families