Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - P62333 (PRS10_HUMAN)

Protein

26S proteasome regulatory subunit 10B

Gene

PSMC6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC6 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.1 Publication

Caution

Alternative initiation from an upstream conserved methionine cannot be fully excluded but is not experimentally supported while initiation from the displayed methionine is supported by PubMed:17323924.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 181ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • proteasome-activating ATPase activity Source: GO_Central
  • protein binding, bridging Source: UniProtKB
  • TBP-class protein binding Source: GO_Central
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1169091 Activation of NF-kappaB in B cells
R-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-HSA-1236974 ER-Phagosome pathway
R-HSA-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174113 SCF-beta-TrCP mediated degradation of Emi1
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-180534 Vpu mediated degradation of CD4
R-HSA-180585 Vif-mediated degradation of APOBEC3G
R-HSA-187577 SCF(Skp2)-mediated degradation of p27/p21
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-202424 Downstream TCR signaling
R-HSA-211733 Regulation of activated PAK-2p34 by proteasome mediated degradation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-HSA-350562 Regulation of ornithine decarboxylase (ODC)
R-HSA-382556 ABC-family proteins mediated transport
R-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-HSA-4608870 Asymmetric localization of PCP proteins
R-HSA-4641257 Degradation of AXIN
R-HSA-4641258 Degradation of DVL
R-HSA-5358346 Hedgehog ligand biogenesis
R-HSA-5362768 Hh mutants that don't undergo autocatalytic processing are degraded by ERAD
R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-5610783 Degradation of GLI2 by the proteasome
R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome
R-HSA-5632684 Hedgehog 'on' state
R-HSA-5658442 Regulation of RAS by GAPs
R-HSA-5668541 TNFR2 non-canonical NF-kB pathway
R-HSA-5676590 NIK-->noncanonical NF-kB signaling
R-HSA-5678895 Defective CFTR causes cystic fibrosis
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-5689603 UCH proteinases
R-HSA-5689880 Ub-specific processing proteases
R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-69229 Ubiquitin-dependent degradation of Cyclin D1
R-HSA-69481 G2/M Checkpoints
R-HSA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-8939902 Regulation of RUNX2 expression and activity
R-HSA-8941858 Regulation of RUNX3 expression and activity
R-HSA-8948751 Regulation of PTEN stability and activity
R-HSA-8951664 Neddylation
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-9020702 Interleukin-1 signaling
R-HSA-9604323 Negative regulation of NOTCH4 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit 10B
Alternative name(s):
26S proteasome AAA-ATPase subunit RPT4
Proteasome 26S subunit ATPase 6
Proteasome subunit p42
Gene namesi
Name:PSMC6
Synonyms:SUG2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000100519.11
HGNCiHGNC:9553 PSMC6
MIMi602708 gene
neXtProtiNX_P62333

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Organism-specific databases

DisGeNETi5706
OpenTargetsiENSG00000100519
PharmGKBiPA33898

Chemistry databases

ChEMBLiCHEMBL2364701

Polymorphism and mutation databases

BioMutaiPSMC6
DMDMi51702772

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000847321 – 38926S proteasome regulatory subunit 10BAdd BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72N6-acetyllysineCombined sources1
Modified residuei206N6-acetyllysineCombined sources1
Modified residuei244PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP62333
MaxQBiP62333
PaxDbiP62333
PeptideAtlasiP62333
PRIDEiP62333
ProteomicsDBi57397

2D gel databases

REPRODUCTION-2DPAGEiIPI00021926

PTM databases

iPTMnetiP62333
PhosphoSitePlusiP62333
SwissPalmiP62333

Expressioni

Gene expression databases

BgeeiENSG00000100519 Expressed in 94 organ(s), highest expression level in kidney
CleanExiHS_PSMC6
ExpressionAtlasiP62333 baseline and differential
GenevisibleiP62333 HS

Organism-specific databases

HPAiHPA042823

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC6 and few additional components (PubMed:27428775, PubMed:27342858). Interacts with PAAF1 (PubMed:15831487).3 Publications

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi111679, 133 interactors
CORUMiP62333
DIPiDIP-38150N
IntActiP62333, 68 interactors
MINTiP62333
STRINGi9606.ENSP00000401802

Structurei

3D structure databases

ProteinModelPortaliP62333
SMRiP62333
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0651 Eukaryota
COG1222 LUCA
GeneTreeiENSGT00550000074826
HOGENOMiHOG000225143
HOVERGENiHBG000109
InParanoidiP62333
KOiK03064
PhylomeDBiP62333
TreeFamiTF106229

Family and domain databases

InterProiView protein in InterPro
IPR005937 26S_Psome_P45-like
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR027417 P-loop_NTPase
IPR035263 PSMC6
PANTHERiPTHR23073:SF59 PTHR23073:SF59, 1 hit
PfamiView protein in Pfam
PF00004 AAA, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01242 26Sp45, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All

P62333-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADPRDKALQ DYRKKLLEHK EIDGRLKELR EQLKELTKQY EKSENDLKAL 
        60         70         80         90        100
QSVGQIVGEV LKQLTEEKFI VKATNGPRYV VGCRRQLDKS KLKPGTRVAL 
       110        120        130        140        150
DMTTLTIMRY LPREVDPLVY NMSHEDPGNV SYSEIGGLSE QIRELREVIE 
       160        170        180        190        200
LPLTNPELFQ RVGIIPPKGC LLYGPPGTGK TLLARAVASQ LDCNFLKVVS 
       210        220        230        240        250
SSIVDKYIGE SARLIREMFN YARDHQPCII FMDEIDAIGG RRFSEGTSAD 
       260        270        280        290        300
REIQRTLMEL LNQMDGFDTL HRVKMIMATN RPDTLDPALL RPGRLDRKIH 
       310        320        330        340        350
IDLPNEQARL DILKIHAGPI TKHGEIDYEA IVKLSDGFNG ADLRNVCTEA 
       360        370        380 
GMFAIRADHD FVVQEDFMKA VRKVADSKKL ESKLDYKPV
Length:389
Mass (Da):44,173
Last modified:July 5, 2004 - v1
Checksum:iB26421295742CACD
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087X2I1A0A087X2I1_HUMAN
26S proteasome regulatory subunit 1...
PSMC6
403Annotation score:
H0YJC0H0YJC0_HUMAN
26S proteasome regulatory subunit 1...
PSMC6
262Annotation score:
H0YJE9H0YJE9_HUMAN
26S proteasome regulatory subunit 1...
PSMC6
70Annotation score:
H0YJT1H0YJT1_HUMAN
26S proteasome regulatory subunit 1...
PSMC6
97Annotation score:
H0YJS8H0YJS8_HUMAN
26S proteasome regulatory subunit 1...
PSMC6
194Annotation score:
H0YJX2H0YJX2_HUMAN
26S proteasome regulatory subunit 1...
PSMC6
67Annotation score:
H0YJD2H0YJD2_HUMAN
26S proteasome regulatory subunit 1...
PSMC6
33Annotation score:
H0YJY8H0YJY8_HUMAN
26S proteasome regulatory subunit 1...
PSMC6
36Annotation score:
H0YJW2H0YJW2_HUMAN
26S proteasome regulatory subunit 1...
PSMC6
14Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti276I → T in BAA11338 (PubMed:8674546).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78275 mRNA Translation: BAA11338.1
AF006305 mRNA Translation: AAB61616.1
BT006843 mRNA Translation: AAP35489.1
CR456709 mRNA Translation: CAG32990.1
AK313670 mRNA Translation: BAG36422.1
CH471078 Genomic DNA Translation: EAW65645.1
BC005390 mRNA Translation: AAH05390.1
PIRiS71316
RefSeqiNP_002797.3, NM_002806.3
UniGeneiHs.156171

Genome annotation databases

EnsembliENST00000606149; ENSP00000475721; ENSG00000100519
GeneIDi5706
KEGGihsa:5706
UCSCiuc059bor.1 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78275 mRNA Translation: BAA11338.1
AF006305 mRNA Translation: AAB61616.1
BT006843 mRNA Translation: AAP35489.1
CR456709 mRNA Translation: CAG32990.1
AK313670 mRNA Translation: BAG36422.1
CH471078 Genomic DNA Translation: EAW65645.1
BC005390 mRNA Translation: AAH05390.1
PIRiS71316
RefSeqiNP_002797.3, NM_002806.3
UniGeneiHs.156171

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GJQelectron microscopy4.50L1-389[»]
5GJRelectron microscopy3.50L/z1-389[»]
5L4Gelectron microscopy4.02L1-389[»]
5LN3electron microscopy6.80L1-389[»]
5M32electron microscopy3.80f1-379[»]
5T0Celectron microscopy3.80AE/BE1-389[»]
5T0Gelectron microscopy4.40E1-389[»]
5T0Helectron microscopy6.80E1-389[»]
5T0Ielectron microscopy8.00E1-389[»]
5T0Jelectron microscopy8.00E1-389[»]
5VFPelectron microscopy4.20E15-389[»]
5VFQelectron microscopy4.20E15-389[»]
5VFRelectron microscopy4.90E15-389[»]
5VFSelectron microscopy3.60E1-389[»]
5VFTelectron microscopy7.00E37-389[»]
5VFUelectron microscopy5.80E37-389[»]
5VGZelectron microscopy3.70E11-114[»]
5VHFelectron microscopy5.70E11-389[»]
5VHHelectron microscopy6.10E11-389[»]
5VHIelectron microscopy6.80E11-389[»]
5VHJelectron microscopy8.50E128-389[»]
5VHMelectron microscopy8.30E128-389[»]
5VHNelectron microscopy7.30E115-389[»]
5VHOelectron microscopy8.30E128-389[»]
5VHPelectron microscopy7.90E128-389[»]
5VHQelectron microscopy8.90E128-389[»]
5VHRelectron microscopy7.70E128-389[»]
5VHSelectron microscopy8.80E11-389[»]
ProteinModelPortaliP62333
SMRiP62333
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111679, 133 interactors
CORUMiP62333
DIPiDIP-38150N
IntActiP62333, 68 interactors
MINTiP62333
STRINGi9606.ENSP00000401802

Chemistry databases

ChEMBLiCHEMBL2364701

PTM databases

iPTMnetiP62333
PhosphoSitePlusiP62333
SwissPalmiP62333

Polymorphism and mutation databases

BioMutaiPSMC6
DMDMi51702772

2D gel databases

REPRODUCTION-2DPAGEiIPI00021926

Proteomic databases

EPDiP62333
MaxQBiP62333
PaxDbiP62333
PeptideAtlasiP62333
PRIDEiP62333
ProteomicsDBi57397

Protocols and materials databases

DNASUi5706
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000606149; ENSP00000475721; ENSG00000100519
GeneIDi5706
KEGGihsa:5706
UCSCiuc059bor.1 human

Organism-specific databases

CTDi5706
DisGeNETi5706
EuPathDBiHostDB:ENSG00000100519.11
GeneCardsiPSMC6
H-InvDBiHIX0011661
HGNCiHGNC:9553 PSMC6
HPAiHPA042823
MIMi602708 gene
neXtProtiNX_P62333
OpenTargetsiENSG00000100519
PharmGKBiPA33898
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0651 Eukaryota
COG1222 LUCA
GeneTreeiENSGT00550000074826
HOGENOMiHOG000225143
HOVERGENiHBG000109
InParanoidiP62333
KOiK03064
PhylomeDBiP62333
TreeFamiTF106229

Enzyme and pathway databases

ReactomeiR-HSA-1169091 Activation of NF-kappaB in B cells
R-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-HSA-1236974 ER-Phagosome pathway
R-HSA-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174113 SCF-beta-TrCP mediated degradation of Emi1
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-180534 Vpu mediated degradation of CD4
R-HSA-180585 Vif-mediated degradation of APOBEC3G
R-HSA-187577 SCF(Skp2)-mediated degradation of p27/p21
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-202424 Downstream TCR signaling
R-HSA-211733 Regulation of activated PAK-2p34 by proteasome mediated degradation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-HSA-350562 Regulation of ornithine decarboxylase (ODC)
R-HSA-382556 ABC-family proteins mediated transport
R-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-HSA-4608870 Asymmetric localization of PCP proteins
R-HSA-4641257 Degradation of AXIN
R-HSA-4641258 Degradation of DVL
R-HSA-5358346 Hedgehog ligand biogenesis
R-HSA-5362768 Hh mutants that don't undergo autocatalytic processing are degraded by ERAD
R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-5610783 Degradation of GLI2 by the proteasome
R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome
R-HSA-5632684 Hedgehog 'on' state
R-HSA-5658442 Regulation of RAS by GAPs
R-HSA-5668541 TNFR2 non-canonical NF-kB pathway
R-HSA-5676590 NIK-->noncanonical NF-kB signaling
R-HSA-5678895 Defective CFTR causes cystic fibrosis
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-5689603 UCH proteinases
R-HSA-5689880 Ub-specific processing proteases
R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-69229 Ubiquitin-dependent degradation of Cyclin D1
R-HSA-69481 G2/M Checkpoints
R-HSA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-8939902 Regulation of RUNX2 expression and activity
R-HSA-8941858 Regulation of RUNX3 expression and activity
R-HSA-8948751 Regulation of PTEN stability and activity
R-HSA-8951664 Neddylation
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-9020702 Interleukin-1 signaling
R-HSA-9604323 Negative regulation of NOTCH4 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiPSMC6 human
GeneWikiiPSMC6
GenomeRNAii5706
PROiPR:P62333
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100519 Expressed in 94 organ(s), highest expression level in kidney
CleanExiHS_PSMC6
ExpressionAtlasiP62333 baseline and differential
GenevisibleiP62333 HS

Family and domain databases

InterProiView protein in InterPro
IPR005937 26S_Psome_P45-like
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR027417 P-loop_NTPase
IPR035263 PSMC6
PANTHERiPTHR23073:SF59 PTHR23073:SF59, 1 hit
PfamiView protein in Pfam
PF00004 AAA, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01242 26Sp45, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPRS10_HUMAN
AccessioniPrimary (citable) accession number: P62333
Secondary accession number(s): B2R975
, P49719, Q6IBU3, Q92524
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: October 10, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again