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Protein

Ubiquitin-conjugating enzyme E2 H

Gene

Ube2h

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.By similarity

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotationBy similarity
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N6-monoubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 H (EC:2.3.2.23)
Alternative name(s):
(E3-independent) E2 ubiquitin-conjugating enzyme H (EC:2.3.2.24)
E2 ubiquitin-conjugating enzyme H
UBCH2
Ubiquitin carrier protein H
Ubiquitin-conjugating enzyme E2-20K
Ubiquitin-protein ligase H
Gene namesi
Name:Ube2h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:104632 Ube2h

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000824871 – 183Ubiquitin-conjugating enzyme E2 HAdd BLAST183

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei60N6-acetyllysineCombined sources1

Post-translational modificationi

Autoubiquitinated in vitro in the presence of NEDD4L.By similarity

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiP62257
PaxDbiP62257
PeptideAtlasiP62257
PRIDEiP62257

PTM databases

iPTMnetiP62257
PhosphoSitePlusiP62257

Expressioni

Gene expression databases

BgeeiENSMUSG00000039159
CleanExiMM_UBE2H
ExpressionAtlasiP62257 baseline and differential
GenevisibleiP62257 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204417, 1 interactor
STRINGi10090.ENSMUSP00000100058

Structurei

3D structure databases

ProteinModelPortaliP62257
SMRiP62257
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0416 Eukaryota
ENOG410XRC5 LUCA
GeneTreeiENSGT00390000004852
HOGENOMiHOG000233452
HOVERGENiHBG063308
InParanoidiP62257
KOiK10576
OMAiNLVNDNM
OrthoDBiEOG091G0RI1
PhylomeDBiP62257
TreeFamiTF101121

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P62257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPSPGKRR MDTDVVKLIE SKHEVTILGG LNEFVVKFYG PQGTPYEGGV
60 70 80 90 100
WKVRVDLPDK YPFKSPSIGF MNKIFHPNID EASGTVCLDV INQTWTALYD
110 120 130 140 150
LTNIFESFLP QLLAYPNPID PLNGDAAAMY LHRPEEYKQK IKEYIQKYAT
160 170 180
EEALKEQEEG TGDSSSESSM SDFSEDEAQD MEL
Length:183
Mass (Da):20,655
Last modified:July 5, 2004 - v1
Checksum:i3DD5D365945F708C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19854 mRNA Translation: AAA91975.1
BC008517 mRNA Translation: AAH08517.1
CCDSiCCDS19969.1
PIRiJC4308
RefSeqiNP_033485.1, NM_009459.3
UniGeneiMm.332967
Mm.391754

Genome annotation databases

EnsembliENSMUST00000102993; ENSMUSP00000100058; ENSMUSG00000039159
GeneIDi22214
KEGGimmu:22214
UCSCiuc009bey.3 mouse

Similar proteinsi

Entry informationi

Entry nameiUBE2H_MOUSE
AccessioniPrimary (citable) accession number: P62257
Secondary accession number(s): P37286
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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