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Protein

Ubiquitin-conjugating enzyme E2 H

Gene

UBE2H

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:8132613, PubMed:17588522, PubMed:20061386). E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex (PubMed:29911972). In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination (PubMed:20061386). Capable, in vitro, to ubiquitinate histone H2A (PubMed:8132613).4 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation5 Publications
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N6-monoubiquitinyl-[acceptor protein]-L-lysine.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation3 Publications
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme activity Source: GO_Central
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B6 2681
ReactomeiR-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiP62256
UniPathwayi
UPA00143

Protein family/group databases

MoonDBiP62256 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 H (EC:2.3.2.235 Publications)
Alternative name(s):
(E3-independent) E2 ubiquitin-conjugating enzyme H (EC:2.3.2.242 Publications)
E2 ubiquitin-conjugating enzyme H
UbcH2
Ubiquitin carrier protein H
Ubiquitin-conjugating enzyme E2-20K
Ubiquitin-protein ligase H
Gene namesi
Name:UBE2H
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000186591.11
HGNCiHGNC:12484 UBE2H
MIMi601082 gene
neXtProtiNX_P62256

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi7328
OpenTargetsiENSG00000186591
PharmGKBiPA37133

Polymorphism and mutation databases

BioMutaiUBE2H
DMDMi51338683

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000824861 – 183Ubiquitin-conjugating enzyme E2 HAdd BLAST183

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei60N6-acetyllysineBy similarity1

Post-translational modificationi

Autoubiquitinated in vitro in the presence of NEDD4L.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiP62256
MaxQBiP62256
PaxDbiP62256
PeptideAtlasiP62256
PRIDEiP62256
ProteomicsDBi57376

PTM databases

iPTMnetiP62256
PhosphoSitePlusiP62256

Expressioni

Gene expression databases

BgeeiENSG00000186591 Expressed in 233 organ(s), highest expression level in secondary oocyte
CleanExiHS_UBE2H
ExpressionAtlasiP62256 baseline and differential
GenevisibleiP62256 HS

Organism-specific databases

HPAiHPA003302

Interactioni

Subunit structurei

Interacts with MAEA and WDR26, components of the CTLH complex that contains GID4, RANBP9 and/or RANBP10, MKLN1, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, ARMC8, WDR26 and YPEL5.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi113176, 90 interactors
IntActiP62256, 50 interactors
STRINGi9606.ENSP00000347836

Structurei

Secondary structure

1183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP62256
SMRiP62256
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62256

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0416 Eukaryota
ENOG410XRC5 LUCA
GeneTreeiENSGT00390000004852
HOGENOMiHOG000233452
HOVERGENiHBG063308
InParanoidiP62256
KOiK10576
OMAiNLVNDNM
OrthoDBiEOG091G0RI1
PhylomeDBiP62256
TreeFamiTF101121

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P62256-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSSPSPGKRR MDTDVVKLIE SKHEVTILGG LNEFVVKFYG PQGTPYEGGV
60 70 80 90 100
WKVRVDLPDK YPFKSPSIGF MNKIFHPNID EASGTVCLDV INQTWTALYD
110 120 130 140 150
LTNIFESFLP QLLAYPNPID PLNGDAAAMY LHRPEEYKQK IKEYIQKYAT
160 170 180
EEALKEQEEG TGDSSSESSM SDFSEDEAQD MEL
Length:183
Mass (Da):20,655
Last modified:July 5, 2004 - v1
Checksum:i3DD5D365945F708C
GO
Isoform 2 (identifier: P62256-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     69-99: Missing.

Note: No experimental confirmation available.
Show »
Length:152
Mass (Da):17,174
Checksum:iC24F6341C98931F0
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9J8Q9C9J8Q9_HUMAN
Ubiquitin-conjugating enzyme E2 H
UBE2H
149Annotation score:
C9JZY6C9JZY6_HUMAN
Ubiquitin-conjugating enzyme E2 H
UBE2H
123Annotation score:
C9JZG9C9JZG9_HUMAN
Ubiquitin-conjugating enzyme E2 H
UBE2H
103Annotation score:
H7C4M9H7C4M9_HUMAN
Ubiquitin-conjugating enzyme E2 H
UBE2H
62Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165S → P in AAP57630 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04458069 – 99Missing in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29328 mRNA Translation: CAA82525.1
Z29330 mRNA Translation: CAA82527.1
Z29331 mRNA Translation: CAA82528.1
AY302138 mRNA Translation: AAP57630.1
BT006756 mRNA Translation: AAP35402.1
AC073320 Genomic DNA No translation available.
AC084865 Genomic DNA No translation available.
CH236950 Genomic DNA Translation: EAL24099.1
CH471070 Genomic DNA Translation: EAW83736.1
BC006277 mRNA Translation: AAH06277.1
CCDSiCCDS47710.1 [P62256-2]
CCDS5814.1 [P62256-1]
PIRiA53516
RefSeqiNP_001189427.1, NM_001202498.1
NP_003335.1, NM_003344.3 [P62256-1]
NP_874356.1, NM_182697.2 [P62256-2]
UniGeneiHs.643548

Genome annotation databases

EnsembliENST00000355621; ENSP00000347836; ENSG00000186591 [P62256-1]
ENST00000473814; ENSP00000419097; ENSG00000186591 [P62256-2]
GeneIDi7328
KEGGihsa:7328
UCSCiuc003vpf.3 human [P62256-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29328 mRNA Translation: CAA82525.1
Z29330 mRNA Translation: CAA82527.1
Z29331 mRNA Translation: CAA82528.1
AY302138 mRNA Translation: AAP57630.1
BT006756 mRNA Translation: AAP35402.1
AC073320 Genomic DNA No translation available.
AC084865 Genomic DNA No translation available.
CH236950 Genomic DNA Translation: EAL24099.1
CH471070 Genomic DNA Translation: EAW83736.1
BC006277 mRNA Translation: AAH06277.1
CCDSiCCDS47710.1 [P62256-2]
CCDS5814.1 [P62256-1]
PIRiA53516
RefSeqiNP_001189427.1, NM_001202498.1
NP_003335.1, NM_003344.3 [P62256-1]
NP_874356.1, NM_182697.2 [P62256-2]
UniGeneiHs.643548

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Z5DX-ray2.10A/B1-160[»]
ProteinModelPortaliP62256
SMRiP62256
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113176, 90 interactors
IntActiP62256, 50 interactors
STRINGi9606.ENSP00000347836

Protein family/group databases

MoonDBiP62256 Predicted

PTM databases

iPTMnetiP62256
PhosphoSitePlusiP62256

Polymorphism and mutation databases

BioMutaiUBE2H
DMDMi51338683

Proteomic databases

EPDiP62256
MaxQBiP62256
PaxDbiP62256
PeptideAtlasiP62256
PRIDEiP62256
ProteomicsDBi57376

Protocols and materials databases

DNASUi7328
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000355621; ENSP00000347836; ENSG00000186591 [P62256-1]
ENST00000473814; ENSP00000419097; ENSG00000186591 [P62256-2]
GeneIDi7328
KEGGihsa:7328
UCSCiuc003vpf.3 human [P62256-1]

Organism-specific databases

CTDi7328
DisGeNETi7328
EuPathDBiHostDB:ENSG00000186591.11
GeneCardsiUBE2H
H-InvDBiHIX0123428
HGNCiHGNC:12484 UBE2H
HPAiHPA003302
MIMi601082 gene
neXtProtiNX_P62256
OpenTargetsiENSG00000186591
PharmGKBiPA37133
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0416 Eukaryota
ENOG410XRC5 LUCA
GeneTreeiENSGT00390000004852
HOGENOMiHOG000233452
HOVERGENiHBG063308
InParanoidiP62256
KOiK10576
OMAiNLVNDNM
OrthoDBiEOG091G0RI1
PhylomeDBiP62256
TreeFamiTF101121

Enzyme and pathway databases

UniPathwayi
UPA00143

BRENDAi2.3.2.B6 2681
ReactomeiR-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiP62256

Miscellaneous databases

ChiTaRSiUBE2H human
EvolutionaryTraceiP62256
GeneWikiiUBE2H
GenomeRNAii7328
PROiPR:P62256
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000186591 Expressed in 233 organ(s), highest expression level in secondary oocyte
CleanExiHS_UBE2H
ExpressionAtlasiP62256 baseline and differential
GenevisibleiP62256 HS

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBE2H_HUMAN
AccessioniPrimary (citable) accession number: P62256
Secondary accession number(s): A4D1L6
, C9JY93, P37286, Q7Z6F4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
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