UniProtKB - P62256 (UBE2H_HUMAN)
Protein
Ubiquitin-conjugating enzyme E2 H
Gene
UBE2H
Organism
Homo sapiens (Human)
Status
Functioni
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:8132613, PubMed:17588522, PubMed:20061386). E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex (PubMed:29911972). In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination (PubMed:20061386). Capable, in vitro, to ubiquitinate histone H2A (PubMed:8132613).4 Publications
Catalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation5 Publications EC:2.3.2.23
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.2 Publications EC:2.3.2.24
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation3 PublicationsView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 87 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ubiquitin conjugating enzyme activity Source: GO_Central
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
- protein K11-linked ubiquitination Source: UniProtKB
- protein K48-linked ubiquitination Source: UniProtKB
- protein polyubiquitination Source: GO_Central
- protein ubiquitination Source: Reactome
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.3.2.B6, 2681 |
PathwayCommonsi | P62256 |
Reactomei | R-HSA-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | P62256 |
UniPathwayi | UPA00143 |
Protein family/group databases
MoonDBi | P62256, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-conjugating enzyme E2 H (EC:2.3.2.235 Publications)Alternative name(s): (E3-independent) E2 ubiquitin-conjugating enzyme H (EC:2.3.2.242 Publications) E2 ubiquitin-conjugating enzyme H UbcH2 Ubiquitin carrier protein H Ubiquitin-conjugating enzyme E2-20K Ubiquitin-protein ligase H |
Gene namesi | Name:UBE2H |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000186591.11 |
HGNCi | HGNC:12484, UBE2H |
MIMi | 601082, gene |
neXtProti | NX_P62256 |
Subcellular locationi
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 7328 |
OpenTargetsi | ENSG00000186591 |
PharmGKBi | PA37133 |
Miscellaneous databases
Pharosi | P62256, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4105951 |
Polymorphism and mutation databases
BioMutai | UBE2H |
DMDMi | 51338683 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082486 | 1 – 183 | Ubiquitin-conjugating enzyme E2 HAdd BLAST | 183 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 60 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Autoubiquitinated in vitro in the presence of NEDD4L.1 Publication
Keywords - PTMi
Acetylation, Ubl conjugationProteomic databases
EPDi | P62256 |
jPOSTi | P62256 |
MassIVEi | P62256 |
MaxQBi | P62256 |
PaxDbi | P62256 |
PeptideAtlasi | P62256 |
PRIDEi | P62256 |
ProteomicsDBi | 57376 [P62256-1] 621 |
PTM databases
iPTMneti | P62256 |
MetOSitei | P62256 |
PhosphoSitePlusi | P62256 |
Expressioni
Gene expression databases
Bgeei | ENSG00000186591, Expressed in secondary oocyte and 245 other tissues |
ExpressionAtlasi | P62256, baseline and differential |
Genevisiblei | P62256, HS |
Organism-specific databases
HPAi | ENSG00000186591, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with MAEA and WDR26, components of the CTLH complex that contains GID4, RANBP9 and/or RANBP10, MKLN1, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, ARMC8, WDR26 and YPEL5.
1 PublicationBinary interactionsi
Hide detailsP62256
With | #Exp. | IntAct |
---|---|---|
MAGEA2B [P43356] | 2 | EBI-2129909,EBI-5650739 |
MAGEC2 [Q9UBF1] | 3 | EBI-2129909,EBI-5651487 |
Protein-protein interaction databases
BioGRIDi | 113176, 99 interactors |
IntActi | P62256, 50 interactors |
STRINGi | 9606.ENSP00000347836 |
Miscellaneous databases
RNActi | P62256, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P62256 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P62256 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 150 | UBC corePROSITE-ProRule annotationAdd BLAST | 150 |
Sequence similaritiesi
Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0416, Eukaryota |
GeneTreei | ENSGT00390000004852 |
InParanoidi | P62256 |
OMAi | EFCVKFY |
PhylomeDBi | P62256 |
TreeFami | TF101121 |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
IDEALi | IID00637 |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P62256-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSPSPGKRR MDTDVVKLIE SKHEVTILGG LNEFVVKFYG PQGTPYEGGV
60 70 80 90 100
WKVRVDLPDK YPFKSPSIGF MNKIFHPNID EASGTVCLDV INQTWTALYD
110 120 130 140 150
LTNIFESFLP QLLAYPNPID PLNGDAAAMY LHRPEEYKQK IKEYIQKYAT
160 170 180
EEALKEQEEG TGDSSSESSM SDFSEDEAQD MEL
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J8Q9 | C9J8Q9_HUMAN | Ubiquitin-conjugating enzyme E2 H | UBE2H | 149 | Annotation score: | ||
A0A3B3IU20 | A0A3B3IU20_HUMAN | Ubiquitin-conjugating enzyme E2 H | UBE2H hCG_18056 | 113 | Annotation score: | ||
C9JZY6 | C9JZY6_HUMAN | Ubiquitin-conjugating enzyme E2 H | UBE2H | 123 | Annotation score: | ||
C9JZG9 | C9JZG9_HUMAN | Ubiquitin-conjugating enzyme E2 H | UBE2H | 103 | Annotation score: | ||
H7C4M9 | H7C4M9_HUMAN | Ubiquitin-conjugating enzyme E2 H | UBE2H | 62 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 165 | S → P in AAP57630 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044580 | 69 – 99 | Missing in isoform 2. 1 PublicationAdd BLAST | 31 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z29328 mRNA Translation: CAA82525.1 Z29330 mRNA Translation: CAA82527.1 Z29331 mRNA Translation: CAA82528.1 AY302138 mRNA Translation: AAP57630.1 BT006756 mRNA Translation: AAP35402.1 AC073320 Genomic DNA No translation available. AC084865 Genomic DNA No translation available. CH236950 Genomic DNA Translation: EAL24099.1 CH471070 Genomic DNA Translation: EAW83736.1 BC006277 mRNA Translation: AAH06277.1 |
CCDSi | CCDS47710.1 [P62256-2] CCDS5814.1 [P62256-1] |
PIRi | A53516 |
RefSeqi | NP_001189427.1, NM_001202498.1 NP_003335.1, NM_003344.3 [P62256-1] NP_874356.1, NM_182697.2 [P62256-2] |
Genome annotation databases
Ensembli | ENST00000355621; ENSP00000347836; ENSG00000186591 [P62256-1] ENST00000473814; ENSP00000419097; ENSG00000186591 [P62256-2] |
GeneIDi | 7328 |
KEGGi | hsa:7328 |
UCSCi | uc003vpf.3, human [P62256-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z29328 mRNA Translation: CAA82525.1 Z29330 mRNA Translation: CAA82527.1 Z29331 mRNA Translation: CAA82528.1 AY302138 mRNA Translation: AAP57630.1 BT006756 mRNA Translation: AAP35402.1 AC073320 Genomic DNA No translation available. AC084865 Genomic DNA No translation available. CH236950 Genomic DNA Translation: EAL24099.1 CH471070 Genomic DNA Translation: EAW83736.1 BC006277 mRNA Translation: AAH06277.1 |
CCDSi | CCDS47710.1 [P62256-2] CCDS5814.1 [P62256-1] |
PIRi | A53516 |
RefSeqi | NP_001189427.1, NM_001202498.1 NP_003335.1, NM_003344.3 [P62256-1] NP_874356.1, NM_182697.2 [P62256-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2Z5D | X-ray | 2.10 | A/B | 1-160 | [»] | |
SMRi | P62256 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 113176, 99 interactors |
IntActi | P62256, 50 interactors |
STRINGi | 9606.ENSP00000347836 |
Chemistry databases
ChEMBLi | CHEMBL4105951 |
Protein family/group databases
MoonDBi | P62256, Predicted |
PTM databases
iPTMneti | P62256 |
MetOSitei | P62256 |
PhosphoSitePlusi | P62256 |
Polymorphism and mutation databases
BioMutai | UBE2H |
DMDMi | 51338683 |
Proteomic databases
EPDi | P62256 |
jPOSTi | P62256 |
MassIVEi | P62256 |
MaxQBi | P62256 |
PaxDbi | P62256 |
PeptideAtlasi | P62256 |
PRIDEi | P62256 |
ProteomicsDBi | 57376 [P62256-1] 621 |
Protocols and materials databases
Antibodypediai | 1149, 201 antibodies |
DNASUi | 7328 |
Genome annotation databases
Ensembli | ENST00000355621; ENSP00000347836; ENSG00000186591 [P62256-1] ENST00000473814; ENSP00000419097; ENSG00000186591 [P62256-2] |
GeneIDi | 7328 |
KEGGi | hsa:7328 |
UCSCi | uc003vpf.3, human [P62256-1] |
Organism-specific databases
CTDi | 7328 |
DisGeNETi | 7328 |
EuPathDBi | HostDB:ENSG00000186591.11 |
GeneCardsi | UBE2H |
HGNCi | HGNC:12484, UBE2H |
HPAi | ENSG00000186591, Low tissue specificity |
MIMi | 601082, gene |
neXtProti | NX_P62256 |
OpenTargetsi | ENSG00000186591 |
PharmGKBi | PA37133 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0416, Eukaryota |
GeneTreei | ENSGT00390000004852 |
InParanoidi | P62256 |
OMAi | EFCVKFY |
PhylomeDBi | P62256 |
TreeFami | TF101121 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
BRENDAi | 2.3.2.B6, 2681 |
PathwayCommonsi | P62256 |
Reactomei | R-HSA-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | P62256 |
Miscellaneous databases
BioGRID-ORCSi | 7328, 305 hits in 850 CRISPR screens |
ChiTaRSi | UBE2H, human |
EvolutionaryTracei | P62256 |
GeneWikii | UBE2H |
GenomeRNAii | 7328 |
Pharosi | P62256, Tbio |
PROi | PR:P62256 |
RNActi | P62256, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000186591, Expressed in secondary oocyte and 245 other tissues |
ExpressionAtlasi | P62256, baseline and differential |
Genevisiblei | P62256, HS |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
IDEALi | IID00637 |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBE2H_HUMAN | |
Accessioni | P62256Primary (citable) accession number: P62256 Secondary accession number(s): A4D1L6 Q7Z6F4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2004 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families