Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

26S proteasome regulatory subunit 4

Gene

PSMC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC1 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi226 – 233ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • proteasome-activating ATPase activity Source: GO_Central
  • RNA binding Source: UniProtKB
  • TBP-class protein binding Source: GO_Central

GO - Biological processi

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1169091 Activation of NF-kappaB in B cells
R-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-HSA-1236974 ER-Phagosome pathway
R-HSA-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174113 SCF-beta-TrCP mediated degradation of Emi1
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-180534 Vpu mediated degradation of CD4
R-HSA-180585 Vif-mediated degradation of APOBEC3G
R-HSA-187577 SCF(Skp2)-mediated degradation of p27/p21
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-202424 Downstream TCR signaling
R-HSA-211733 Regulation of activated PAK-2p34 by proteasome mediated degradation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-HSA-350562 Regulation of ornithine decarboxylase (ODC)
R-HSA-382556 ABC-family proteins mediated transport
R-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-HSA-4608870 Asymmetric localization of PCP proteins
R-HSA-4641257 Degradation of AXIN
R-HSA-4641258 Degradation of DVL
R-HSA-532668 N-glycan trimming in the ER and Calnexin/Calreticulin cycle
R-HSA-5358346 Hedgehog ligand biogenesis
R-HSA-5362768 Hh mutants that don't undergo autocatalytic processing are degraded by ERAD
R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-5610783 Degradation of GLI2 by the proteasome
R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome
R-HSA-5632684 Hedgehog 'on' state
R-HSA-5658442 Regulation of RAS by GAPs
R-HSA-5668541 TNFR2 non-canonical NF-kB pathway
R-HSA-5676590 NIK-->noncanonical NF-kB signaling
R-HSA-5678895 Defective CFTR causes cystic fibrosis
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-5689603 UCH proteinases
R-HSA-5689880 Ub-specific processing proteases
R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-69229 Ubiquitin-dependent degradation of Cyclin D1
R-HSA-69481 G2/M Checkpoints
R-HSA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-8939902 Regulation of RUNX2 expression and activity
R-HSA-8941858 Regulation of RUNX3 expression and activity
R-HSA-8948751 Regulation of PTEN stability and activity
R-HSA-8951664 Neddylation
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-9020702 Interleukin-1 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit 4
Short name:
P26s4
Alternative name(s):
26S proteasome AAA-ATPase subunit RPT2
Proteasome 26S subunit ATPase 1
Gene namesi
Name:PSMC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000100764.13
HGNCiHGNC:9547 PSMC1
MIMi602706 gene
neXtProtiNX_P62191

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus, Proteasome

Pathology & Biotechi

Organism-specific databases

DisGeNETi5700
OpenTargetsiENSG00000100764
PharmGKBiPA33892

Chemistry databases

ChEMBLiCHEMBL2364701

Polymorphism and mutation databases

BioMutaiPSMC1
DMDMi49065817

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources3 Publications
ChainiPRO_00000846772 – 44026S proteasome regulatory subunit 4Add BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine3 Publications1
Modified residuei4PhosphoserineCombined sources1
Modified residuei53PhosphothreonineCombined sources1
Cross-linki237Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei258N6-acetyllysineCombined sources1
Modified residuei434PhosphothreonineCombined sources1
Modified residuei439PhosphotyrosineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP62191
PaxDbiP62191
PeptideAtlasiP62191
PRIDEiP62191
ProteomicsDBi57370

PTM databases

CarbonylDBiP62191
iPTMnetiP62191
PhosphoSitePlusiP62191
SwissPalmiP62191

Expressioni

Gene expression databases

BgeeiENSG00000100764
CleanExiHS_PSMC1
ExpressionAtlasiP62191 baseline and differential
GenevisibleiP62191 HS

Organism-specific databases

HPAiHPA000872

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC1 and few additional components (PubMed:27428775, PubMed:27342858). Interacts with SCA7 (PubMed:11734547). Interacts with NGLY1 (PubMed:15358861). Interacts with PAAF1 (PubMed:15831487).5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111673, 144 interactors
CORUMiP62191
IntActiP62191, 81 interactors
MINTiP62191
STRINGi9606.ENSP00000261303

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GJQelectron microscopy4.50I1-440[»]
5GJRelectron microscopy3.50I/w1-440[»]
5L4Gelectron microscopy4.02I1-440[»]
5LN3electron microscopy6.80I1-440[»]
5M32electron microscopy3.80d1-428[»]
5T0Celectron microscopy3.80AB/BB1-440[»]
5T0Gelectron microscopy4.40B1-440[»]
5T0Helectron microscopy6.80B1-440[»]
5T0Ielectron microscopy8.00B1-440[»]
5T0Jelectron microscopy8.00B1-440[»]
5VGZelectron microscopy3.70B93-165[»]
5VHFelectron microscopy5.70B93-432[»]
5VHHelectron microscopy6.10B93-432[»]
5VHIelectron microscopy6.80B93-433[»]
5VHJelectron microscopy8.50B167-432[»]
5VHMelectron microscopy8.30B167-432[»]
5VHNelectron microscopy7.30B167-432[»]
5VHOelectron microscopy8.30B167-433[»]
5VHPelectron microscopy7.90B167-432[»]
5VHQelectron microscopy8.90B167-433[»]
5VHRelectron microscopy7.70B167-433[»]
5VHSelectron microscopy8.80B93-433[»]
ProteinModelPortaliP62191
SMRiP62191
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0726 Eukaryota
COG1222 LUCA
GeneTreeiENSGT00550000074818
HOGENOMiHOG000225143
HOVERGENiHBG000109
InParanoidiP62191
KOiK03062
OMAiNATFIRV
OrthoDBiEOG091G07NC
PhylomeDBiP62191
TreeFamiTF106226

Family and domain databases

InterProiView protein in InterPro
IPR005937 26S_Psome_P45-like
IPR035244 26S_subunit_4
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR027417 P-loop_NTPase
PANTHERiPTHR23073:SF24 PTHR23073:SF24, 1 hit
PfamiView protein in Pfam
PF00004 AAA, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01242 26Sp45, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62191-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQSQSGGHG PGGGKKDDKD KKKKYEPPVP TRVGKKKKKT KGPDAASKLP
60 70 80 90 100
LVTPHTQCRL KLLKLERIKD YLLMEEEFIR NQEQMKPLEE KQEEERSKVD
110 120 130 140 150
DLRGTPMSVG TLEEIIDDNH AIVSTSVGSE HYVSILSFVD KDLLEPGCSV
160 170 180 190 200
LLNHKVHAVI GVLMDDTDPL VTVMKVEKAP QETYADIGGL DNQIQEIKES
210 220 230 240 250
VELPLTHPEY YEEMGIKPPK GVILYGPPGT GKTLLAKAVA NQTSATFLRV
260 270 280 290 300
VGSELIQKYL GDGPKLVREL FRVAEEHAPS IVFIDEIDAI GTKRYDSNSG
310 320 330 340 350
GEREIQRTML ELLNQLDGFD SRGDVKVIMA TNRIETLDPA LIRPGRIDRK
360 370 380 390 400
IEFPLPDEKT KKRIFQIHTS RMTLADDVTL DDLIMAKDDL SGADIKAICT
410 420 430 440
EAGLMALRER RMKVTNEDFK KSKENVLYKK QEGTPEGLYL
Length:440
Mass (Da):49,185
Last modified:June 21, 2004 - v1
Checksum:iACA80782F4F96F49
GO
Isoform 2 (identifier: P62191-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.

Note: No experimental confirmation available.
Show »
Length:367
Mass (Da):41,167
Checksum:iF96ACDAF2907971C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19K → E in AAA35484 (PubMed:1429620).Curated1
Sequence conflicti70D → G in AAH67741 (PubMed:15489334).Curated1
Sequence conflicti120H → R in AAH16368 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0557681 – 73Missing in isoform 2. 1 PublicationAdd BLAST73

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02426 mRNA Translation: AAA35484.1
AK299121 mRNA Translation: BAG61175.1
CR457044 mRNA Translation: CAG33325.1
AL161662 Genomic DNA No translation available.
AL355074 Genomic DNA No translation available.
BC000512 mRNA Translation: AAH00512.1
BC016368 mRNA Translation: AAH16368.1
BC067741 mRNA Translation: AAH67741.1
BC073818 mRNA Translation: AAH73818.1
CCDSiCCDS32139.1 [P62191-1]
CCDS81837.1 [P62191-2]
PIRiA44468
RefSeqiNP_001317141.1, NM_001330212.1 [P62191-2]
NP_002793.2, NM_002802.2 [P62191-1]
XP_016876959.1, XM_017021470.1 [P62191-2]
UniGeneiHs.356654
Hs.476697

Genome annotation databases

EnsembliENST00000261303; ENSP00000261303; ENSG00000100764 [P62191-1]
ENST00000543772; ENSP00000445147; ENSG00000100764 [P62191-2]
GeneIDi5700
KEGGihsa:5700
UCSCiuc001xyg.4 human [P62191-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPRS4_HUMAN
AccessioniPrimary (citable) accession number: P62191
Secondary accession number(s): B4DR63
, P49014, Q03527, Q6IAW0, Q6NW36, Q96AZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 21, 2004
Last modified: June 20, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health