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Protein

Neuronal calcium sensor 1

Gene

Ncs1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.1 Publication

Miscellaneous

Fourfold reduction in calcium affinity for NCS1/FREQ in the presence of magnesium.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi73 – 841Add BLAST12
Calcium bindingi109 – 1202Add BLAST12
Calcium bindingi157 – 1683Add BLAST12

GO - Molecular functioni

  • calcium-dependent protein kinase inhibitor activity Source: UniProtKB
  • calcium ion binding Source: RGD
  • magnesium ion binding Source: RGD
  • protein kinase binding Source: RGD
  • voltage-gated calcium channel activity Source: UniProtKB
  • voltage-gated calcium channel activity involved in regulation of presynaptic cytosolic calcium levels Source: SynGO

GO - Biological processi

Keywordsi

LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal calcium sensor 1
Short name:
NCS-1
Alternative name(s):
Frequenin homolog
Frequenin-like protein
Frequenin-like ubiquitous protein
Gene namesi
Name:Ncs1
Synonyms:Flup, Freq
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi68417 Ncs1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73D → A: Loss of magnesium-binding; when associated with Q-84, A-109 and Q-120. 1 Publication1
Mutagenesisi84E → Q: Loss of magnesium-binding; when associated with A-73, A-109 and Q-120. 1 Publication1
Mutagenesisi109D → A: Loss of magnesium-binding; when associated with A-73, Q-84 and Q-120. 1 Publication1
Mutagenesisi120E → Q: Loss of magnesium-binding; when associated with A-73, Q-84 and A-109. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000737902 – 190Neuronal calcium sensor 1Add BLAST189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP62168
PRIDEiP62168

PTM databases

iPTMnetiP62168

Expressioni

Gene expression databases

BgeeiENSRNOG00000008761 Expressed in 9 organ(s), highest expression level in brain
GenevisibleiP62168 RN

Interactioni

Subunit structurei

Interacts in a calcium-independent manner with PI4KB, but only if myristoylated. This binding competes with CALN2/CABP7 binding to PI4KB. Interacts in a calcium-dependent manner with PICK1 (via AH domain). Interacts with KCND2, ARF1, ARF3, ARF5 and ARF6 (By similarity). Interacts with IL1RAPL1 (By similarity).By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

IntActiP62168, 7 interactors
STRINGi10116.ENSRNOP00000011648

Structurei

Secondary structure

1190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP62168
SMRiP62168
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 59EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini60 – 95EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini96 – 131EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini144 – 179EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 47Ancestral calcium site 1Add BLAST12
Regioni174 – 190Interaction with IL1RAPL1By similarityAdd BLAST17

Domaini

Binds 3 calcium ions via the second, third and fourth EF-hand. EF-2 and EF-3 bind both magnesium and calcium, while EF-4 binds only calcium. At the resting state, magnesium is bound to EF-2 and EF-3 and upon activation, both sites bind calcium simultaneously while EF-4 is the last one to be occupied.

Sequence similaritiesi

Belongs to the recoverin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00760000118820
HOGENOMiHOG000233019
HOVERGENiHBG108179
InParanoidiP62168
KOiK19932
OMAiVQSIYKM
OrthoDBiEOG091G11T4
PhylomeDBiP62168
TreeFamiTF300009

Family and domain databases

CDDicd00051 EFh, 2 hits
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF00036 EF-hand_1, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 3 hits
PS50222 EF_HAND_2, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62168-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK
60 70 80 90 100
IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK
110 120 130 140 150
LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD
160 170 180 190
RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV
Length:190
Mass (Da):21,879
Last modified:January 23, 2007 - v2
Checksum:i9AF8E26A23F80D4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27421 mRNA Translation: AAA88510.1
X82188 mRNA Translation: CAA57678.1
RefSeqiNP_077342.1, NM_024366.1
UniGeneiRn.62653

Genome annotation databases

EnsembliENSRNOT00000011648; ENSRNOP00000011648; ENSRNOG00000008761
GeneIDi65153
KEGGirno:65153
UCSCiRGD:68417 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27421 mRNA Translation: AAA88510.1
X82188 mRNA Translation: CAA57678.1
RefSeqiNP_077342.1, NM_024366.1
UniGeneiRn.62653

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OV2X-ray2.60A/B/C/D1-177[»]
4YRUX-ray2.80A/B/C/D1-177[»]
5AEQX-ray1.95A/B1-190[»]
5AERX-ray2.19A1-190[»]
5AFPX-ray2.30A/B1-190[»]
ProteinModelPortaliP62168
SMRiP62168
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP62168, 7 interactors
STRINGi10116.ENSRNOP00000011648

PTM databases

iPTMnetiP62168

Proteomic databases

PaxDbiP62168
PRIDEiP62168

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011648; ENSRNOP00000011648; ENSRNOG00000008761
GeneIDi65153
KEGGirno:65153
UCSCiRGD:68417 rat

Organism-specific databases

CTDi23413
RGDi68417 Ncs1

Phylogenomic databases

eggNOGiKOG0044 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00760000118820
HOGENOMiHOG000233019
HOVERGENiHBG108179
InParanoidiP62168
KOiK19932
OMAiVQSIYKM
OrthoDBiEOG091G11T4
PhylomeDBiP62168
TreeFamiTF300009

Miscellaneous databases

PROiPR:P62168

Gene expression databases

BgeeiENSRNOG00000008761 Expressed in 9 organ(s), highest expression level in brain
GenevisibleiP62168 RN

Family and domain databases

CDDicd00051 EFh, 2 hits
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF00036 EF-hand_1, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 3 hits
PS50222 EF_HAND_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNCS1_RAT
AccessioniPrimary (citable) accession number: P62168
Secondary accession number(s): P36610, Q9UK26
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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