UniProtKB - P62168 (NCS1_RAT)
Neuronal calcium sensor 1
Ncs1
Functioni
Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.
1 PublicationMiscellaneous
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 73 | Calcium 1PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 75 | Calcium 1PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 77 | Calcium 1PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 79 | Calcium 1; via carbonyl oxygenPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 81 | Calcium 1Combined sources1 Publication | 1 | |
Metal bindingi | 84 | Calcium 1PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 109 | Calcium 2PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 111 | Calcium 2PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 113 | Calcium 2PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 115 | Calcium 2; via carbonyl oxygenPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 120 | Calcium 2PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 157 | Calcium 3PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 159 | Calcium 3PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 161 | Calcium 3PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 163 | Calcium 3; via carbonyl oxygenPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 168 | Calcium 3PROSITE-ProRule annotationCombined sources1 Publication | 1 |
GO - Molecular functioni
- calcium-dependent protein kinase inhibitor activity Source: UniProtKB
- calcium ion binding Source: RGD
- calcium sensitive guanylate cyclase activator activity Source: GO_Central
- magnesium ion binding Source: RGD
- protein kinase binding Source: RGD
- voltage-gated calcium channel activity Source: UniProtKB
- voltage-gated calcium channel activity involved in regulation of presynaptic cytosolic calcium levels Source: SynGO
GO - Biological processi
- phosphatidylinositol-mediated signaling Source: RGD
- positive regulation of exocytosis Source: RGD
- regulation of neuron projection development Source: UniProtKB
- regulation of synaptic vesicle exocytosis Source: SynGO
Keywordsi
Ligand | Calcium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Neuronal calcium sensor 1Short name: NCS-1 Alternative name(s): Frequenin homolog Frequenin-like protein Frequenin-like ubiquitous protein |
Gene namesi | Name:Ncs1 Synonyms:Flup, Freq |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 68417, Ncs1 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Golgi apparatus
- Golgi apparatus 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- postsynaptic density Curated
- perinuclear region By similarity
- Membrane By similarity1 Publication; Lipid-anchor By similarity
Note: Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions.By similarityCurated1 Publication
Cytosol
- cytosol Source: RGD
- postsynaptic cytosol Source: SynGO
- presynaptic cytosol Source: SynGO
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: RGD
Other locations
- axon Source: RGD
- calyx of Held Source: SynGO
- cytoplasm Source: RGD
- cytoplasmic vesicle Source: RGD
- dendrite Source: RGD
- glutamatergic synapse Source: SynGO
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- postsynapse Source: SynGO
- postsynaptic density Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 73 | D → A: Loss of magnesium-binding; when associated with Q-84, A-109 and Q-120. 1 Publication | 1 | |
Mutagenesisi | 84 | E → Q: Loss of magnesium-binding; when associated with A-73, A-109 and Q-120. 1 Publication | 1 | |
Mutagenesisi | 109 | D → A: Loss of magnesium-binding; when associated with A-73, Q-84 and Q-120. 1 Publication | 1 | |
Mutagenesisi | 120 | E → Q: Loss of magnesium-binding; when associated with A-73, Q-84 and A-109. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000073790 | 2 – 190 | Neuronal calcium sensor 1Add BLAST | 189 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 |
Keywords - PTMi
Lipoprotein, MyristateProteomic databases
jPOSTi | P62168 |
PaxDbi | P62168 |
PTM databases
iPTMneti | P62168 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000008761, Expressed in frontal cortex and 21 other tissues |
Genevisiblei | P62168, RN |
Interactioni
Subunit structurei
Monomer (PubMed:25979333).
Interacts with KCND2 (By similarity).
Interacts in a calcium-independent manner with PI4KB, but only if myristoylated (PubMed:11526106). This binding competes with CALN2/CABP7 binding to PI4KB (PubMed:11526106).
Interacts in a calcium-dependent manner with PICK1 (via AH domain) (PubMed:19109914).
Interacts with ARF1, ARF3, ARF5 and ARF6 (By similarity).
Interacts with IL1RAPL1 (By similarity).
By similarity3 PublicationsBinary interactionsi
P62168
With | #Exp. | IntAct |
---|---|---|
ARF1 [P84080] from Bos taurus. | 2 | EBI-907774,EBI-449051 |
PDE1A [P14100] from Bos taurus. | 2 | EBI-907774,EBI-907809 |
GO - Molecular functioni
- protein kinase binding Source: RGD
Protein-protein interaction databases
IntActi | P62168, 7 interactors |
STRINGi | 10116.ENSRNOP00000011648 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P62168 |
BMRBi | P62168 |
SMRi | P62168 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 59 | EF-hand 1CuratedAdd BLAST | 36 | |
Domaini | 60 – 95 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 96 – 131 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 144 – 179 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 36 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 36 – 47 | Ancestral calcium site 1Add BLAST | 12 | |
Regioni | 174 – 190 | Interaction with IL1RAPL1By similarityAdd BLAST | 17 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0044, Eukaryota |
GeneTreei | ENSGT00940000154645 |
HOGENOMi | CLU_072366_1_2_1 |
InParanoidi | P62168 |
OMAi | EYVFNVF |
OrthoDBi | 1369072at2759 |
PhylomeDBi | P62168 |
TreeFami | TF300009 |
Family and domain databases
CDDi | cd00051, EFh, 2 hits |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR028846, Recoverin |
PANTHERi | PTHR23055, PTHR23055, 1 hit |
Pfami | View protein in Pfam PF00036, EF-hand_1, 1 hit PF13499, EF-hand_7, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 3 hits PS50222, EF_HAND_2, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK
60 70 80 90 100
IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK
110 120 130 140 150
LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD
160 170 180 190
RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A8I6AM90 | A0A8I6AM90_RAT | Neuronal calcium sensor 1 | Ncs1 | 223 | Annotation score: | ||
A0A8I6GJM4 | A0A8I6GJM4_RAT | Neuronal calcium sensor 1 | Ncs1 | 202 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L27421 mRNA Translation: AAA88510.1 X82188 mRNA Translation: CAA57678.1 |
RefSeqi | NP_077342.1, NM_024366.1 |
Genome annotation databases
Ensembli | ENSRNOT00000111270; ENSRNOP00000091251; ENSRNOG00000064701 |
GeneIDi | 65153 |
KEGGi | rno:65153 |
UCSCi | RGD:68417, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L27421 mRNA Translation: AAA88510.1 X82188 mRNA Translation: CAA57678.1 |
RefSeqi | NP_077342.1, NM_024366.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4OV2 | X-ray | 2.60 | A/B/C/D | 1-177 | [»] | |
4YRU | X-ray | 2.80 | A/B/C/D | 1-177 | [»] | |
5AEQ | X-ray | 1.95 | A/B | 1-190 | [»] | |
5AER | X-ray | 2.19 | A | 1-190 | [»] | |
5AFP | X-ray | 2.30 | A/B | 1-190 | [»] | |
AlphaFoldDBi | P62168 | |||||
BMRBi | P62168 | |||||
SMRi | P62168 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P62168, 7 interactors |
STRINGi | 10116.ENSRNOP00000011648 |
PTM databases
iPTMneti | P62168 |
Proteomic databases
jPOSTi | P62168 |
PaxDbi | P62168 |
Genome annotation databases
Ensembli | ENSRNOT00000111270; ENSRNOP00000091251; ENSRNOG00000064701 |
GeneIDi | 65153 |
KEGGi | rno:65153 |
UCSCi | RGD:68417, rat |
Organism-specific databases
CTDi | 23413 |
RGDi | 68417, Ncs1 |
Phylogenomic databases
eggNOGi | KOG0044, Eukaryota |
GeneTreei | ENSGT00940000154645 |
HOGENOMi | CLU_072366_1_2_1 |
InParanoidi | P62168 |
OMAi | EYVFNVF |
OrthoDBi | 1369072at2759 |
PhylomeDBi | P62168 |
TreeFami | TF300009 |
Miscellaneous databases
PROi | PR:P62168 |
Gene expression databases
Bgeei | ENSRNOG00000008761, Expressed in frontal cortex and 21 other tissues |
Genevisiblei | P62168, RN |
Family and domain databases
CDDi | cd00051, EFh, 2 hits |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR028846, Recoverin |
PANTHERi | PTHR23055, PTHR23055, 1 hit |
Pfami | View protein in Pfam PF00036, EF-hand_1, 1 hit PF13499, EF-hand_7, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 3 hits PS50222, EF_HAND_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | NCS1_RAT | |
Accessioni | P62168Primary (citable) accession number: P62168 Secondary accession number(s): P36610, Q9UK26 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 21, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 151 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families