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Protein

Neuronal calcium sensor 1

Gene

NCS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel (By similarity).By similarity

Miscellaneous

Binds 3 calcium ions via the second, third and fourth EF-hand.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi73 – 8411 PublicationAdd BLAST12
Calcium bindingi109 – 12021 PublicationAdd BLAST12
Calcium bindingi157 – 16831 PublicationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal calcium sensor 1
Short name:
NCS-1
Alternative name(s):
Frequenin homolog
Frequenin-like protein
Frequenin-like ubiquitous protein
Gene namesi
Name:NCS1
Synonyms:FLUP, FREQ
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000107130.9
HGNCiHGNC:3953 NCS1
MIMi603315 gene
neXtProtiNX_P62166

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi81E → T: Reduces calcium binding; when associated with A-117 or A-165. Abolishes calcium binding; when associated with A-117 and A-165. 1 Publication1
Mutagenesisi117T → A: Reduces calcium binding; when associated with T-81. Abolishes calcium binding; when associated with T-81 and A-165. 1 Publication1
Mutagenesisi165T → A: Reduces calcium binding; when associated with A-117. Abolishes calcium binding; when associated with T-81 and A-117. 1 Publication1

Organism-specific databases

DisGeNETi23413
OpenTargetsiENSG00000107130
PharmGKBiPA28371

Polymorphism and mutation databases

BioMutaiNCS1
DMDMi49065666

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000737882 – 190Neuronal calcium sensor 1Add BLAST189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

EPDiP62166
MaxQBiP62166
PaxDbiP62166
PeptideAtlasiP62166
PRIDEiP62166
ProteomicsDBi57369
TopDownProteomicsiP62166-1 [P62166-1]

PTM databases

iPTMnetiP62166
PhosphoSitePlusiP62166

Expressioni

Gene expression databases

BgeeiENSG00000107130 Expressed in 184 organ(s), highest expression level in dorsolateral prefrontal cortex
CleanExiHS_FREQ
ExpressionAtlasiP62166 baseline and differential
GenevisibleiP62166 HS

Organism-specific databases

HPAiCAB018587
HPA019713

Interactioni

Subunit structurei

Interacts with KCND2. Interacts in a calcium-independent manner with PI4KB. This binding competes with CALN2/CABP7 binding to PI4KB (By similarity). Interacts with ARF1, ARF3, ARF5 and ARF6. Interacts in a calcium-dependent manner with PICK1 (via AH domain) (By similarity). Interacts with IL1RAPL1.By similarity3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116985, 33 interactors
IntActiP62166, 17 interactors
MINTiP62166
STRINGi9606.ENSP00000361475

Structurei

Secondary structure

1190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP62166
SMRiP62166
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62166

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 59EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini60 – 95EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini96 – 131EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini144 – 179EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni174 – 190Interaction with IL1RAPL11 PublicationAdd BLAST17

Sequence similaritiesi

Belongs to the recoverin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00760000118820
HOGENOMiHOG000233019
HOVERGENiHBG108179
InParanoidiP62166
KOiK19932
OMAiVQSIYKM
OrthoDBiEOG091G11T4
PhylomeDBiP62166
TreeFamiTF300009

Family and domain databases

CDDicd00051 EFh, 2 hits
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF00036 EF-hand_1, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 3 hits
PS50222 EF_HAND_2, 3 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: P62166-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK
60 70 80 90 100
IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK
110 120 130 140 150
LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD
160 170 180 190
RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV
Length:190
Mass (Da):21,879
Last modified:January 23, 2007 - v2
Checksum:i9AF8E26A23F80D4F
GO
Isoform 2 (identifier: P62166-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MGKSNSKLKPEVVEELTRKTYF → MATI

Note: No experimental confirmation available.
Show »
Length:172
Mass (Da):19,728
Checksum:i8A8063D0D705465C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90S → P in AAF01804 (Ref. 4) Curated1
Sequence conflicti178S → P in AAF01804 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0463121 – 22MGKSN…RKTYF → MATI in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84048 mRNA Translation: CAA58867.1
AF134479 mRNA Translation: AAP97256.1
AF186409 mRNA Translation: AAF01804.1
AL360004 Genomic DNA No translation available.
BC004856 mRNA Translation: AAH04856.1
BQ880305 mRNA No translation available.
CCDSiCCDS6932.1 [P62166-1]
CCDS78448.1 [P62166-2]
RefSeqiNP_001122298.1, NM_001128826.1 [P62166-2]
NP_055101.2, NM_014286.3 [P62166-1]
UniGeneiHs.642946
Hs.714951

Genome annotation databases

EnsembliENST00000372398; ENSP00000361475; ENSG00000107130 [P62166-1]
ENST00000630865; ENSP00000486695; ENSG00000107130 [P62166-2]
GeneIDi23413
KEGGihsa:23413
UCSCiuc004bzi.2 human [P62166-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84048 mRNA Translation: CAA58867.1
AF134479 mRNA Translation: AAP97256.1
AF186409 mRNA Translation: AAF01804.1
AL360004 Genomic DNA No translation available.
BC004856 mRNA Translation: AAH04856.1
BQ880305 mRNA No translation available.
CCDSiCCDS6932.1 [P62166-1]
CCDS78448.1 [P62166-2]
RefSeqiNP_001122298.1, NM_001128826.1 [P62166-2]
NP_055101.2, NM_014286.3 [P62166-1]
UniGeneiHs.642946
Hs.714951

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G8IX-ray1.90A/B1-190[»]
2LCPNMR-A1-190[»]
4GUKX-ray1.75A/B/C/D6-188[»]
5O9SX-ray2.70C/D2-9[»]
ProteinModelPortaliP62166
SMRiP62166
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116985, 33 interactors
IntActiP62166, 17 interactors
MINTiP62166
STRINGi9606.ENSP00000361475

PTM databases

iPTMnetiP62166
PhosphoSitePlusiP62166

Polymorphism and mutation databases

BioMutaiNCS1
DMDMi49065666

Proteomic databases

EPDiP62166
MaxQBiP62166
PaxDbiP62166
PeptideAtlasiP62166
PRIDEiP62166
ProteomicsDBi57369
TopDownProteomicsiP62166-1 [P62166-1]

Protocols and materials databases

DNASUi23413
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372398; ENSP00000361475; ENSG00000107130 [P62166-1]
ENST00000630865; ENSP00000486695; ENSG00000107130 [P62166-2]
GeneIDi23413
KEGGihsa:23413
UCSCiuc004bzi.2 human [P62166-1]

Organism-specific databases

CTDi23413
DisGeNETi23413
EuPathDBiHostDB:ENSG00000107130.9
GeneCardsiNCS1
HGNCiHGNC:3953 NCS1
HPAiCAB018587
HPA019713
MIMi603315 gene
neXtProtiNX_P62166
OpenTargetsiENSG00000107130
PharmGKBiPA28371
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0044 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00760000118820
HOGENOMiHOG000233019
HOVERGENiHBG108179
InParanoidiP62166
KOiK19932
OMAiVQSIYKM
OrthoDBiEOG091G11T4
PhylomeDBiP62166
TreeFamiTF300009

Miscellaneous databases

ChiTaRSiNCS1 human
EvolutionaryTraceiP62166
GeneWikiiNeuronal_calcium_sensor-1
GenomeRNAii23413
PROiPR:P62166
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000107130 Expressed in 184 organ(s), highest expression level in dorsolateral prefrontal cortex
CleanExiHS_FREQ
ExpressionAtlasiP62166 baseline and differential
GenevisibleiP62166 HS

Family and domain databases

CDDicd00051 EFh, 2 hits
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF00036 EF-hand_1, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 3 hits
PS50222 EF_HAND_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNCS1_HUMAN
AccessioniPrimary (citable) accession number: P62166
Secondary accession number(s): E9PAY3, P36610, Q9UK26
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
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