UniProtKB - P62141 (PP1B_MOUSE)
Serine/threonine-protein phosphatase PP1-beta catalytic subunit
Ppp1cb
Functioni
Catalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 63 | Manganese 1By similarity | 1 | |
Metal bindingi | 65 | Manganese 1By similarity | 1 | |
Metal bindingi | 91 | Manganese 1By similarity | 1 | |
Metal bindingi | 91 | Manganese 2By similarity | 1 | |
Metal bindingi | 123 | Manganese 2By similarity | 1 | |
Active sitei | 124 | Proton donorBy similarity | 1 | |
Metal bindingi | 172 | Manganese 2By similarity | 1 | |
Metal bindingi | 247 | Manganese 2By similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- myosin-light-chain-phosphatase activity Source: UniProtKB
- myosin phosphatase activity Source: UniProtKB
- phosphatase activity Source: UniProtKB
- phosphoprotein phosphatase activity Source: MGI
- protein kinase binding Source: MGI
- protein serine/threonine phosphatase activity Source: MGI
- protein serine phosphatase activity Source: UniProtKB-EC
- protein threonine phosphatase activity Source: UniProtKB-EC
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- circadian regulation of gene expression Source: UniProtKB
- entrainment of circadian clock by photoperiod Source: UniProtKB
- glycogen metabolic process Source: UniProtKB-KW
- protein dephosphorylation Source: UniProtKB
- regulation of cell adhesion Source: UniProtKB
- regulation of circadian rhythm Source: UniProtKB
- regulation of glycogen biosynthetic process Source: MGI
- regulation of glycogen catabolic process Source: MGI
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Biological process | Biological rhythms, Carbohydrate metabolism, Cell cycle, Cell division, Glycogen metabolism |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-5625740, RHO GTPases activate PKNs R-MMU-5627123, RHO GTPases activate PAKs R-MMU-5673000, RAF activation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ppp1cb |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104871, Ppp1cb |
Subcellular locationi
Nucleus
- Nucleus By similarity
- nucleoplasm By similarity
- nucleolus By similarity
Other locations
- Cytoplasm By similarity
Note: Highly mobile in cells and can be relocalized through interaction with targeting subunits. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear chromosome, telomeric region Source: Ensembl
- nucleolus Source: UniProtKB-SubCell
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: MGI
- glycogen granule Source: MGI
- protein phosphatase type 1 complex Source: MGI
- PTW/PP1 phosphatase complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000058780 | 2 – 327 | Serine/threonine-protein phosphatase PP1-beta catalytic subunitAdd BLAST | 326 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 316 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P62141 |
jPOSTi | P62141 |
MaxQBi | P62141 |
PaxDbi | P62141 |
PeptideAtlasi | P62141 |
PRIDEi | P62141 |
PTM databases
iPTMneti | P62141 |
PhosphoSitePlusi | P62141 |
SwissPalmi | P62141 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000014956, Expressed in blood and 306 other tissues |
Genevisiblei | P62141, MM |
Interactioni
Subunit structurei
PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. The targeting or regulatory subunits determine the substrate specificity of PP1. PPP1R12A, PPP1R12B and PPP1R12C mediate binding to myosin. PPP1R3A (in skeletal muscle), PPP1R3B (in liver), PPP1R3C, PPP1R3D and PPP1R3F (in brain) mediate binding to glycogen.
Interacts with PPP1R7 and PPP1R12C.
Interacts with PPP1R12A and NUAK1; the interaction is direct.
Interacts with TRIM28; the interaction is weak (By similarity).
Interacts with PPP1R15A; the interaction mediates binding to EIF2S1.
Interacts with PPP1R16B.
Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA or PPP1CB or PPP1CC.
Interacts with PPP1R8.
Interacts with TRIM28; the interaction is weak (By similarity).
Part of a complex containing PPP1R15B, PP1 and NCK1/2.
Interacts with PPP1R15B; the interaction mediates binding to EIF2S1.
Interacts with FOXP3 (By similarity).
Interacts with RRP1B (By similarity).
Interacts with SERPINE1.
Interacts with LZTR1 (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202336, 96 interactors |
IntActi | P62141, 83 interactors |
MINTi | P62141 |
STRINGi | 10090.ENSMUSP00000015100 |
Miscellaneous databases
RNActi | P62141, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0374, Eukaryota |
GeneTreei | ENSGT00940000154644 |
HOGENOMi | CLU_004962_0_0_1 |
InParanoidi | P62141 |
OMAi | NVRIWKT |
OrthoDBi | 766640at2759 |
PhylomeDBi | P62141 |
TreeFami | TF354243 |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR006186, Ser/Thr-sp_prot-phosphatase IPR031675, STPPase_N |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit PF16891, STPPase_N, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MADGELNVDS LITRLLEVRG CRPGKIVQMT EAEVRGLCIK SREIFLSQPI
60 70 80 90 100
LLELEAPLKI CGDIHGQYTD LLRLFEYGGF PPEANYLFLG DYVDRGKQSL
110 120 130 140 150
ETICLLLAYK IKYPENFFLL RGNHECASIN RIYGFYDECK RRFNIKLWKT
160 170 180 190 200
FTDCFNCLPI AAIVDEKIFC CHGGLSPDLQ SMEQIRRIMR PTDVPDTGLL
210 220 230 240 250
CDLLWSDPDK DVQGWGENDR GVSFTFGADV VSKFLNRHDL DLICRAHQVV
260 270 280 290 300
EDGYEFFAKR QLVTLFSAPN YCGEFDNAGG MMSVDETLMC SFQILKPSEK
310 320
KAKYQYGGLN SGRPVTPPRT ANPPKKR
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0J9YUU8 | A0A0J9YUU8_MOUSE | Serine/threonine-protein phosphatas... | Ppp1cb | 100 | Annotation score: | ||
A0A0J9YUG2 | A0A0J9YUG2_MOUSE | Serine/threonine-protein phosphatas... | Ppp1cb | 59 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 64 | I → N in BAC40733 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 148 | W → R in BAE42366 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 223 | S → F in BAE38902 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 250 | V → E in BAB23473 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 269 | P → S in BAE42366 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27073 mRNA Translation: AAA37527.1 AK004686 mRNA Translation: BAB23473.1 AK088893 mRNA Translation: BAC40636.1 AK089067 mRNA Translation: BAC40733.1 AK147112 mRNA Translation: BAE27684.1 AK160743 mRNA Translation: BAE35984.1 AK166168 mRNA Translation: BAE38608.1 AK166623 mRNA Translation: BAE38902.1 AK168141 mRNA Translation: BAE40108.1 AK169379 mRNA Translation: BAE41126.1 AK171283 mRNA Translation: BAE42366.1 BC046832 mRNA Translation: AAH46832.1 |
CCDSi | CCDS19193.1 |
PIRi | D32550 |
RefSeqi | NP_766295.2, NM_172707.3 |
Genome annotation databases
Ensembli | ENSMUST00000015100; ENSMUSP00000015100; ENSMUSG00000014956 ENSMUST00000201360; ENSMUSP00000144047; ENSMUSG00000014956 |
GeneIDi | 19046 |
KEGGi | mmu:19046 |
UCSCi | uc008wzq.2, mouse |
Similar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight The things we forget - Issue 32 of March 2003 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27073 mRNA Translation: AAA37527.1 AK004686 mRNA Translation: BAB23473.1 AK088893 mRNA Translation: BAC40636.1 AK089067 mRNA Translation: BAC40733.1 AK147112 mRNA Translation: BAE27684.1 AK160743 mRNA Translation: BAE35984.1 AK166168 mRNA Translation: BAE38608.1 AK166623 mRNA Translation: BAE38902.1 AK168141 mRNA Translation: BAE40108.1 AK169379 mRNA Translation: BAE41126.1 AK171283 mRNA Translation: BAE42366.1 BC046832 mRNA Translation: AAH46832.1 |
CCDSi | CCDS19193.1 |
PIRi | D32550 |
RefSeqi | NP_766295.2, NM_172707.3 |
3D structure databases
SMRi | P62141 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202336, 96 interactors |
IntActi | P62141, 83 interactors |
MINTi | P62141 |
STRINGi | 10090.ENSMUSP00000015100 |
PTM databases
iPTMneti | P62141 |
PhosphoSitePlusi | P62141 |
SwissPalmi | P62141 |
Proteomic databases
EPDi | P62141 |
jPOSTi | P62141 |
MaxQBi | P62141 |
PaxDbi | P62141 |
PeptideAtlasi | P62141 |
PRIDEi | P62141 |
Protocols and materials databases
Antibodypediai | 28856, 480 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000015100; ENSMUSP00000015100; ENSMUSG00000014956 ENSMUST00000201360; ENSMUSP00000144047; ENSMUSG00000014956 |
GeneIDi | 19046 |
KEGGi | mmu:19046 |
UCSCi | uc008wzq.2, mouse |
Organism-specific databases
CTDi | 5500 |
MGIi | MGI:104871, Ppp1cb |
Phylogenomic databases
eggNOGi | KOG0374, Eukaryota |
GeneTreei | ENSGT00940000154644 |
HOGENOMi | CLU_004962_0_0_1 |
InParanoidi | P62141 |
OMAi | NVRIWKT |
OrthoDBi | 766640at2759 |
PhylomeDBi | P62141 |
TreeFami | TF354243 |
Enzyme and pathway databases
Reactomei | R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-5625740, RHO GTPases activate PKNs R-MMU-5627123, RHO GTPases activate PAKs R-MMU-5673000, RAF activation |
Miscellaneous databases
BioGRID-ORCSi | 19046, 9 hits in 17 CRISPR screens |
ChiTaRSi | Ppp1cb, mouse |
PROi | PR:P62141 |
RNActi | P62141, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000014956, Expressed in blood and 306 other tissues |
Genevisiblei | P62141, MM |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR006186, Ser/Thr-sp_prot-phosphatase IPR031675, STPPase_N |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit PF16891, STPPase_N, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PP1B_MOUSE | |
Accessioni | P62141Primary (citable) accession number: P62141 Secondary accession number(s): P37140 Q9DBY2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 21, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 172 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries