UniProtKB - P62137 (PP1A_MOUSE)
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Ppp1ca
Functioni
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
- Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 64 | Manganese 1By similarity | 1 | |
Metal bindingi | 66 | Manganese 1By similarity | 1 | |
Metal bindingi | 92 | Manganese 1By similarity | 1 | |
Metal bindingi | 92 | Manganese 2By similarity | 1 | |
Metal bindingi | 124 | Manganese 2By similarity | 1 | |
Active sitei | 125 | Proton donorBy similarity | 1 | |
Metal bindingi | 173 | Manganese 2By similarity | 1 | |
Metal bindingi | 248 | Manganese 2By similarity | 1 |
GO - Molecular functioni
- cadherin binding involved in cell-cell adhesion Source: MGI
- metal ion binding Source: UniProtKB-KW
- phosphatase activity Source: UniProtKB
- phosphoprotein phosphatase activity Source: MGI
- protein-containing complex binding Source: MGI
- protein phosphatase 1 binding Source: MGI
- protein serine/threonine phosphatase activity Source: MGI
- ribonucleoprotein complex binding Source: MGI
GO - Biological processi
- branching morphogenesis of an epithelial tube Source: MGI
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cellular process Source: MGI
- circadian regulation of gene expression Source: UniProtKB
- dephosphorylation Source: MGI
- entrainment of circadian clock by photoperiod Source: UniProtKB
- glycogen metabolic process Source: MGI
- lung development Source: MGI
- positive regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- protein dephosphorylation Source: UniProtKB
- regulation of circadian rhythm Source: UniProtKB
- regulation of glycogen biosynthetic process Source: MGI
- regulation of glycogen catabolic process Source: MGI
- regulation of translation Source: MGI
- response to lead ion Source: MGI
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Biological process | Biological rhythms, Carbohydrate metabolism, Cell cycle, Cell division, Glycogen metabolism |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-180024, DARPP-32 events |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (EC:3.1.3.16)Short name: PP-1A |
Gene namesi | Name:Ppp1ca Synonyms:Ppp1a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:103016, Ppp1ca |
Subcellular locationi
Nucleus
- Nucleus By similarity
- nucleoplasm By similarity
- nucleolus By similarity
Other locations
- Cytoplasm By similarity
Note: Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles (By similarity).By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear chromosome, telomeric region Source: Ensembl
- nucleolus Source: UniProtKB-SubCell
- nucleoplasm Source: MGI
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- adherens junction Source: MGI
- cytoplasm Source: MGI
- dendritic spine Source: MGI
- glutamatergic synapse Source: MGI
- glycogen granule Source: MGI
- neuron projection Source: MGI
- neuronal cell body Source: MGI
- perikaryon Source: MGI
- postsynapse Source: MGI
- presynapse Source: MGI
- protein phosphatase type 1 complex Source: MGI
- PTW/PP1 phosphatase complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 64 | D → N: Dominant negatif, severely disrupts circadian rhythmicity of transcription. 1 Publication | 1 | |
Mutagenesisi | 95 | D → N: Dominant negatif, severely disrupts circadian rhythmicity of transcription. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000058775 | 2 – 330 | Serine/threonine-protein phosphatase PP1-alpha catalytic subunitAdd BLAST | 329 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 2 | PhosphoserineBy similarity | 1 | |
Modified residuei | 22 | PhosphoserineBy similarity | 1 | |
Modified residuei | 305 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 306 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 320 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 325 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P62137 |
jPOSTi | P62137 |
MaxQBi | P62137 |
PaxDbi | P62137 |
PeptideAtlasi | P62137 |
PRIDEi | P62137 |
PTM databases
iPTMneti | P62137 |
PhosphoSitePlusi | P62137 |
SwissPalmi | P62137 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000040385, Expressed in thymus and 287 other tissues |
Genevisiblei | P62137, MM |
Interactioni
Subunit structurei
PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A, PPP1R12B and PPP1R12C mediate binding to myosin. PPP1R3A (in skeletal muscle), PPP1R3B (in liver), PPP1R3C, PPP1R3D and PPP1R3F (in brain) mediate binding to glycogen.
Interacts with PPP1R9A, PPP1R9B and PPP1R7.
Interacts with PPP1R15A; the interaction mediates binding to EIF2S1.
Interacts with YLPM1.
Forms a complex with ILF2, ILF3, YLPM1, KHDRBS1, RBMX and NCOA5.
Interacts with NOM1 and PPP1R8.
Interacts with PPP1R16B. Interacts. with RPSA only in the presence of PPP1R16B.
Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA or PPP1CB or PPP1CC.
Interacts with PPP1R10/PNUTS and PPP1R8.
Interacts with WDR82 in the presence of PPP1R10/PNUTS.
Interacts with PPP1R39.
Interacts with TRIM28; the interaction dephosphorylates TRIM28 on 'Ser-824' and forms a complex at the p21 promoter site (By similarity).
Interacts with PPP1R15B; the interaction mediates binding to EIF2S1.
Part of a complex containing PPP1R15B, PP1 and NCK1/2.
Interacts with NEK2.
Interacts with FER; this promotes phosphorylation at Thr-320 (By similarity).
Interacts with PHACTR4; which acts as an activator of PP1 activity.
Interacts with BTBD10 (PubMed:18160256).
Interacts with KCTD20 (PubMed:24156551).
Interacts with FOXP3 (By similarity).
Interacts with CENPA (By similarity).
Interacts with ATG16L1 (By similarity).
Found in a complex with PPP1CA, PPP1CC, SHC1 and PEAK1 (By similarity).
By similarity5 PublicationsBinary interactionsi
Hide detailsP62137
GO - Molecular functioni
- cadherin binding involved in cell-cell adhesion Source: MGI
- protein phosphatase 1 binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202335, 170 interactors |
IntActi | P62137, 169 interactors |
MINTi | P62137 |
STRINGi | 10090.ENSMUSP00000039109 |
Miscellaneous databases
RNActi | P62137, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P62137 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0374, Eukaryota |
GeneTreei | ENSGT00940000153472 |
HOGENOMi | CLU_004962_8_1_1 |
InParanoidi | P62137 |
OMAi | YLVMESR |
OrthoDBi | 766640at2759 |
PhylomeDBi | P62137 |
TreeFami | TF354243 |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR006186, Ser/Thr-sp_prot-phosphatase IPR031675, STPPase_N |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit PF16891, STPPase_N, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP
60 70 80 90 100
ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS
110 120 130 140 150
LETICLLLAY KIRYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK
160 170 180 190 200
TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL
210 220 230 240 250
LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV
260 270 280 290 300
VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD
310 320 330
KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25809 mRNA Translation: AAC99814.1 AK007932 mRNA Translation: BAB25358.1 AK028392 mRNA Translation: BAC25928.1 AK090070 mRNA Translation: BAC41078.1 AK151582 mRNA Translation: BAE30522.1 AK152667 mRNA Translation: BAE31402.1 AK153517 mRNA Translation: BAE32060.1 AK159575 mRNA Translation: BAE35196.1 AK167244 mRNA Translation: BAE39365.1 AK167538 mRNA Translation: BAE39605.1 AK167880 mRNA Translation: BAE39893.1 AK167981 mRNA Translation: BAE39973.1 BC014828 mRNA Translation: AAH14828.1 |
CCDSi | CCDS29421.1 |
RefSeqi | NP_114074.1, NM_031868.2 |
Genome annotation databases
Ensembli | ENSMUST00000046094; ENSMUSP00000039109; ENSMUSG00000040385 ENSMUST00000180575; ENSMUSP00000137997; ENSMUSG00000096994 |
GeneIDi | 19045 |
KEGGi | mmu:19045 |
UCSCi | uc008fzg.1, mouse |
Similar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight The things we forget - Issue 32 of March 2003 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25809 mRNA Translation: AAC99814.1 AK007932 mRNA Translation: BAB25358.1 AK028392 mRNA Translation: BAC25928.1 AK090070 mRNA Translation: BAC41078.1 AK151582 mRNA Translation: BAE30522.1 AK152667 mRNA Translation: BAE31402.1 AK153517 mRNA Translation: BAE32060.1 AK159575 mRNA Translation: BAE35196.1 AK167244 mRNA Translation: BAE39365.1 AK167538 mRNA Translation: BAE39605.1 AK167880 mRNA Translation: BAE39893.1 AK167981 mRNA Translation: BAE39973.1 BC014828 mRNA Translation: AAH14828.1 |
CCDSi | CCDS29421.1 |
RefSeqi | NP_114074.1, NM_031868.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5ZQV | X-ray | 2.95 | A/B/C/D | 1-330 | [»] | |
5ZT0 | X-ray | 3.32 | A/B/C/D/E/F | 7-300 | [»] | |
SMRi | P62137 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202335, 170 interactors |
IntActi | P62137, 169 interactors |
MINTi | P62137 |
STRINGi | 10090.ENSMUSP00000039109 |
PTM databases
iPTMneti | P62137 |
PhosphoSitePlusi | P62137 |
SwissPalmi | P62137 |
Proteomic databases
EPDi | P62137 |
jPOSTi | P62137 |
MaxQBi | P62137 |
PaxDbi | P62137 |
PeptideAtlasi | P62137 |
PRIDEi | P62137 |
Protocols and materials databases
Antibodypediai | 3872, 586 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000046094; ENSMUSP00000039109; ENSMUSG00000040385 ENSMUST00000180575; ENSMUSP00000137997; ENSMUSG00000096994 |
GeneIDi | 19045 |
KEGGi | mmu:19045 |
UCSCi | uc008fzg.1, mouse |
Organism-specific databases
CTDi | 5499 |
MGIi | MGI:103016, Ppp1ca |
Phylogenomic databases
eggNOGi | KOG0374, Eukaryota |
GeneTreei | ENSGT00940000153472 |
HOGENOMi | CLU_004962_8_1_1 |
InParanoidi | P62137 |
OMAi | YLVMESR |
OrthoDBi | 766640at2759 |
PhylomeDBi | P62137 |
TreeFami | TF354243 |
Enzyme and pathway databases
Reactomei | R-MMU-180024, DARPP-32 events |
Miscellaneous databases
BioGRID-ORCSi | 19045, 4 hits in 17 CRISPR screens |
ChiTaRSi | Ppp1ca, mouse |
PROi | PR:P62137 |
RNActi | P62137, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040385, Expressed in thymus and 287 other tissues |
Genevisiblei | P62137, MM |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR006186, Ser/Thr-sp_prot-phosphatase IPR031675, STPPase_N |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit PF16891, STPPase_N, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PP1A_MOUSE | |
Accessioni | P62137Primary (citable) accession number: P62137 Secondary accession number(s): P08129 Q9Z1G2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | June 21, 2004 | |
Last modified: | December 2, 2020 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries