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Protein

Phosphatase and actin regulator 1

Gene

Phactr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds actin monomers (G actin) and plays a role in the reorganization of the actin cytoskeleton and in formation of actin stress fibers. Plays a role in cell motility. Plays a role in the formation of tubules by endothelial cells. Regulates PPP1CA activity. Required for normal cell survival (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Protein phosphatase inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatase and actin regulator 1
Gene namesi
Name:Phactr1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1303187 Phactr1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Nucleus, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi576R → A: No effect on PPP1CA-binding. 1 Publication1
Mutagenesisi577F → A: Strongly reduces PPP1CA-binding. 1 Publication1
Mutagenesisi578H → A: Reduces PPP1CA-binding. 1 Publication1
Mutagenesisi579R → A: No effect on PPP1CA-binding. 1 Publication1
Mutagenesisi580P → A: No effect on PPP1CA-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001266351 – 580Phosphatase and actin regulator 1Add BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67PhosphoserineBy similarity1
Modified residuei78PhosphoserineBy similarity1
Modified residuei104PhosphothreonineBy similarity1
Modified residuei467PhosphoserineBy similarity1
Modified residuei505PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP62024
PRIDEiP62024

PTM databases

iPTMnetiP62024
PhosphoSitePlusiP62024

Expressioni

Tissue specificityi

Selectively expressed in brain. High levels are found in the olfactory tubercle, nucleus accumbens core and shell, caudate-putamen, cerebral cortex, hippocampus and piriform cortex. Moderate to high levels in the olfactory bulb, arcuate and ventromedial hypothalamus, subthalamic nucleus, amygdala, lateral septum, habenula and thalamus. Low expression, if any, in substantia nigra pars compacta/pars reticula and globus pallidus (at protein level).1 Publication

Interactioni

Subunit structurei

Interacts (via RPEL repeats) with ACTA1 and PPP1CA; ACTA1 and PPP1CA compete for the same binding site.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

ELMiP62024
STRINGi10116.ENSRNOP00000043053

Structurei

3D structure databases

SMRiP62024
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati138 – 163RPEL 1Add BLAST26
Repeati422 – 447RPEL 2Add BLAST26
Repeati460 – 485RPEL 3Add BLAST26
Repeati498 – 523RPEL 4Add BLAST26

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi108 – 129Nuclear localization signalBy similarityAdd BLAST22

Domaini

Binds three actin monomers via the three C-terminal RPEL repeats.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4339 Eukaryota
ENOG4111M01 LUCA
HOGENOMiHOG000220879
HOVERGENiHBG057352
InParanoidiP62024
PhylomeDBiP62024

Family and domain databases

InterProiView protein in InterPro
IPR029987 Phactr1
IPR004018 RPEL_repeat
PANTHERiPTHR12751:SF6 PTHR12751:SF6, 1 hit
PfamiView protein in Pfam
PF02755 RPEL, 4 hits
SMARTiView protein in SMART
SM00707 RPEL, 4 hits
PROSITEiView protein in PROSITE
PS51073 RPEL, 4 hits

Sequencei

Sequence statusi: Complete.

P62024-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDYPKMDYFL DVESAHRLLD VESAQRFFYS QGAQARRATL LLPPTLMAAS
60 70 80 90 100
SEDDIDRRPI RRVRSKSDTP YLAEARISFN LGAAEEVERL AAMRSDSLVP
110 120 130 140 150
GTHTPPIRRR SKFANLGRIF KPWKWRKKKS EKFKHTSAAL ERKISMRQSR
160 170 180 190 200
EELIKRGVLK EIYDKDGELS ISNEDDSLTN GQSLSSSQLS LPALSEMEPV
210 220 230 240 250
PMPRDPCSYE VLQASDIMDG PDPGAPVKLP CLPVKLSPPL PPKKVLICMP
260 270 280 290 300
VGGPELSLAS YAAQKSSQQA VAQHHHTVLP SQMQHQLQYG SHGQHLPSST
310 320 330 340 350
GTLPMHPSGC RMIDELNKTL AMTMQRLESS EQRVPCSTSY HSSGLHSSDG
360 370 380 390 400
VTKAGPLGLP EIRQVPTVVI ECDDNKENVP HEPDYEDSSC LYAREEEEEE
410 420 430 440 450
EDEDDDASLY TSSLAMKVCR KDSLAIKLSN RPSKRELEEK NILPRQTDEE
460 470 480 490 500
RLELRQQIGT KLTRRLSQRP TAEELEQRNI LKPRNEQEEQ EEKREIKRRL
510 520 530 540 550
TRKLSQRPTV EELRERKILI RFSDYVEVAD AQDYDRRADK PWTRLTAADK
560 570 580
AAIRKELNEF KSTEMEVHEL SRHLTRFHRP
Length:580
Mass (Da):66,216
Last modified:June 7, 2004 - v1
Checksum:i443E6E7DDA22F381
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti262A → T in AAH98634 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY494977 mRNA Translation: AAS77487.1
BC098634 mRNA Translation: AAH98634.1
RefSeqiNP_999622.2, NM_214457.2
UniGeneiRn.22981

Genome annotation databases

GeneIDi306844
KEGGirno:306844
UCSCiRGD:1303187 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY494977 mRNA Translation: AAS77487.1
BC098634 mRNA Translation: AAH98634.1
RefSeqiNP_999622.2, NM_214457.2
UniGeneiRn.22981

3D structure databases

SMRiP62024
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ELMiP62024
STRINGi10116.ENSRNOP00000043053

PTM databases

iPTMnetiP62024
PhosphoSitePlusiP62024

Proteomic databases

PaxDbiP62024
PRIDEiP62024

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi306844
KEGGirno:306844
UCSCiRGD:1303187 rat

Organism-specific databases

CTDi221692
RGDi1303187 Phactr1

Phylogenomic databases

eggNOGiKOG4339 Eukaryota
ENOG4111M01 LUCA
HOGENOMiHOG000220879
HOVERGENiHBG057352
InParanoidiP62024
PhylomeDBiP62024

Miscellaneous databases

PROiPR:P62024

Family and domain databases

InterProiView protein in InterPro
IPR029987 Phactr1
IPR004018 RPEL_repeat
PANTHERiPTHR12751:SF6 PTHR12751:SF6, 1 hit
PfamiView protein in Pfam
PF02755 RPEL, 4 hits
SMARTiView protein in SMART
SM00707 RPEL, 4 hits
PROSITEiView protein in PROSITE
PS51073 RPEL, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPHAR1_RAT
AccessioniPrimary (citable) accession number: P62024
Secondary accession number(s): Q4KMC2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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