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Protein

14-3-3 protein gamma

Gene

YWHAG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.1 Publication

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • insulin-like growth factor receptor binding Source: UniProtKB
  • protein domain specific binding Source: Ensembl
  • protein kinase C binding Source: UniProtKB
  • protein kinase C inhibitor activity Source: UniProtKB
  • receptor tyrosine kinase binding Source: Ensembl
  • RNA binding Source: UniProtKB

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-111447 Activation of BAD and translocation to mitochondria
R-HSA-1445148 Translocation of GLUT4 to the plasma membrane
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-380259 Loss of Nlp from mitotic centrosomes
R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes
R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome
R-HSA-380320 Recruitment of NuMA to mitotic centrosomes
R-HSA-5620912 Anchoring of the basal body to the plasma membrane
R-HSA-5625740 RHO GTPases activate PKNs
R-HSA-5628897 TP53 Regulates Metabolic Genes
R-HSA-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
R-HSA-8854518 AURKA Activation by TPX2
SignaLinkiP61981
SIGNORiP61981

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein gamma
Alternative name(s):
Protein kinase C inhibitor protein 1
Short name:
KCIP-1
Cleaved into the following chain:
Gene namesi
Name:YWHAG
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000170027.6
HGNCiHGNC:12852 YWHAG
MIMi605356 gene
neXtProtiNX_P61981

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Involvement in diseasei

Epileptic encephalopathy, early infantile, 56 (EIEE56)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of epileptic encephalopathy, a heterogeneous group of severe childhood onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. EIEE56 is an autosomal dominant condition.
See also OMIM:617665
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_08022415E → A in EIEE56; unknown pathological significance. 1 Publication1
Natural variantiVAR_080226129D → E in EIEE56. 1 Publication1
Natural variantiVAR_080227132R → C in EIEE56. 1 Publication1

Keywords - Diseasei

Disease mutation, Epilepsy

Organism-specific databases

DisGeNETi7532
MalaCardsiYWHAG
MIMi617665 phenotype
OpenTargetsiENSG00000170027
PharmGKBiPA37441

Chemistry databases

ChEMBLiCHEMBL1293296

Polymorphism and mutation databases

BioMutaiYWHAG
DMDMi48428721

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003679071 – 24714-3-3 protein gammaAdd BLAST247
Initiator methionineiRemoved; alternateCombined sources4 Publications
ChainiPRO_00000586062 – 24714-3-3 protein gamma, N-terminally processedAdd BLAST246

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine; in 14-3-3 protein gamma; alternate; partial1 Publication1
Modified residuei2N-acetylvaline; in 14-3-3 protein gamma, N-terminally processed; partialCombined sources3 Publications1
Modified residuei2N-acetylvaline; partialCombined sources3 Publications1
Modified residuei71PhosphoserineCombined sources1
Modified residuei133PhosphotyrosineBy similarity1
Modified residuei145Phosphothreonine1 Publication1
Modified residuei215PhosphoserineBy similarity1
Modified residuei234PhosphothreonineCombined sources1
Modified residuei235PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by various PKC isozymes.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP61981
MaxQBiP61981
PaxDbiP61981
PeptideAtlasiP61981
PRIDEiP61981
TopDownProteomicsiP61981

2D gel databases

REPRODUCTION-2DPAGEiIPI00220642

PTM databases

iPTMnetiP61981
PhosphoSitePlusiP61981
SwissPalmiP61981

Expressioni

Tissue specificityi

Highly expressed in brain, skeletal muscle, and heart.1 Publication

Gene expression databases

BgeeiENSG00000170027
CleanExiHS_YWHAG
GenevisibleiP61981 HS

Organism-specific databases

HPAiCAB013274
CAB018389
HPA026918

Interactioni

Subunit structurei

Homodimer. Interacts with SAMSN1 (By similarity). Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT and SIRT2.Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (PubMed:23572552). Interacts with SLITRK1 (PubMed:19640509).By similarity12 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Interaction with phosphoserine on interacting protein1
Sitei132Interaction with phosphoserine on interacting protein1

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • insulin-like growth factor receptor binding Source: UniProtKB
  • protein domain specific binding Source: Ensembl
  • protein kinase C binding Source: UniProtKB
  • receptor tyrosine kinase binding Source: Ensembl

Protein-protein interaction databases

BioGridi113364, 323 interactors
CORUMiP61981
DIPiDIP-33406N
ELMiP61981
IntActiP61981, 131 interactors
MINTiP61981
STRINGi9606.ENSP00000306330

Chemistry databases

BindingDBiP61981

Structurei

Secondary structure

1247
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 16Combined sources13
Helixi20 – 31Combined sources12
Turni32 – 34Combined sources3
Helixi39 – 70Combined sources32
Turni76 – 78Combined sources3
Helixi79 – 106Combined sources28
Helixi108 – 111Combined sources4
Helixi117 – 137Combined sources21
Helixi140 – 164Combined sources25
Helixi170 – 185Combined sources16
Helixi190 – 206Combined sources17
Helixi207 – 210Combined sources4
Turni213 – 215Combined sources3
Helixi216 – 233Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B05X-ray2.55A/B/C/D/E/F2-247[»]
3UZDX-ray1.86A1-247[»]
4E2EX-ray2.25A1-247[»]
4J6SX-ray3.08A/B/C/D1-247[»]
4O46X-ray2.90A/B/C/D/E/F1-247[»]
5D3EX-ray2.75A/B/E/F/I/J1-238[»]
6BYJX-ray2.90A/B/C/D/E/F2-241[»]
6BYLX-ray3.35A/B/C/D/E/F2-241[»]
6BZDX-ray2.67A/B/C/D2-247[»]
6FELX-ray2.84A/B/C/D1-234[»]
ProteinModelPortaliP61981
SMRiP61981
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61981

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841 Eukaryota
COG5040 LUCA
GeneTreeiENSGT00760000119116
HOGENOMiHOG000240379
HOVERGENiHBG050423
InParanoidiP61981
KOiK16198
OMAiIKNCGET
OrthoDBiEOG091G0VKY
PhylomeDBiP61981
TreeFamiTF102003

Family and domain databases

Gene3Di1.20.190.20, 1 hit
InterProiView protein in InterPro
IPR000308 14-3-3
IPR023409 14-3-3_CS
IPR036815 14-3-3_dom_sf
IPR023410 14-3-3_domain
PANTHERiPTHR18860 PTHR18860, 1 hit
PfamiView protein in Pfam
PF00244 14-3-3, 1 hit
PIRSFiPIRSF000868 14-3-3, 1 hit
PRINTSiPR00305 1433ZETA
SMARTiView protein in SMART
SM00101 14_3_3, 1 hit
SUPFAMiSSF48445 SSF48445, 1 hit
PROSITEiView protein in PROSITE
PS00796 1433_1, 1 hit
PS00797 1433_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61981-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK
60 70 80 90 100
NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV
110 120 130 140 150
LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS
160 170 180 190 200
EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA
210 220 230 240
FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN
Length:247
Mass (Da):28,303
Last modified:January 23, 2007 - v2
Checksum:iB0D16C6DE1F4455D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4R → P in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti19R → G in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti78Missing in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti89I → V in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti104L → V in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti109I → Y in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti119 – 122SKVF → RKDL in AAD48408 (PubMed:10433554).Curated4
Sequence conflicti144 – 145AT → GD in AAD48408 (PubMed:10433554).Curated2
Sequence conflicti157 – 158AH → R in AAD48408 (PubMed:10433554).Curated2
Sequence conflicti200 – 202AFD → EFE in AAD48408 (PubMed:10433554).Curated3
Sequence conflicti214D → E in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti240D → DH in AAD48408 (PubMed:10433554).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_08022415E → A in EIEE56; unknown pathological significance. 1 Publication1
Natural variantiVAR_08022550K → Q Found in an individual with autism; unknown pathological significance. 1 Publication1
Natural variantiVAR_080226129D → E in EIEE56. 1 Publication1
Natural variantiVAR_080227132R → C in EIEE56. 1 Publication1
Natural variantiVAR_080228133Y → S Probable disease-associated mutation found in an individual with neurodevelopmental disorder. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142498 mRNA Translation: AAD48408.1
AB024334 mRNA Translation: BAA85184.1
CR541904 mRNA Translation: CAG46702.1
CR541925 mRNA Translation: CAG46723.1
AC006388 Genomic DNA No translation available.
BC020963 mRNA Translation: AAH20963.1
CCDSiCCDS5584.1
RefSeqiNP_036611.2, NM_012479.3
UniGeneiHs.744840

Genome annotation databases

EnsembliENST00000307630; ENSP00000306330; ENSG00000170027
GeneIDi7532
KEGGihsa:7532
UCSCiuc011kgj.2 human

Similar proteinsi

Entry informationi

Entry namei1433G_HUMAN
AccessioniPrimary (citable) accession number: P61981
Secondary accession number(s): O70457
, P35214, Q6FH52, Q9UDP2, Q9UN99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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