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Protein

Nuclear transport factor 2

Gene

Nutf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import.2 Publications

GO - Molecular functioni

  • identical protein binding Source: Ensembl
  • nuclear import signal receptor activity Source: UniProtKB
  • protein transporter activity Source: Ensembl
  • Ran GTPase binding Source: UniProtKB
  • structural constituent of nuclear pore Source: Ensembl

GO - Biological processi

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear transport factor 2Curated
Short name:
NTF-2
Gene namesi
Name:Nutf2Imported
Synonyms:Ntf21 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi1359213 Nutf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7W → A: No effect on interaction with GDP-bound RAN. Decreased interaction with nucleoporins. Decreased localization to the nuclear pore complex. Decreased GDP-bound RAN and other proteins nuclear import. 1 Publication1
Mutagenesisi19Y → A: Loss of interaction with GDP-bound RAN. Loss of GDP-bound RAN nuclear import. 1 Publication1
Mutagenesisi23D → A or N: No effect on interaction with GDP-bound RAN. Increases GDP-bound RAN nuclear import. Increased interaction with nucleoporins and localization to the nuclear pore complex. Inhibits the nuclear import of nuclear localization signal-containing proteins. 2 Publications1
Mutagenesisi23D → N: No effect on interaction with GDP-bound RAN. No effect on interaction with nucleoporins. No effect on proteins nuclear import. 1 Publication1
Mutagenesisi42E → D: Loss of interaction with GDP-bound RAN. Loss of GDP-bound RAN nuclear import. 1 Publication1
Mutagenesisi42E → K: Loss of interaction with GDP-bound RAN. No effect on interaction with nucleoporins. Loss of GDP-bound RAN and other proteins nuclear import. 2 Publications1
Mutagenesisi64I → A: No effect on homodimerization. Decreased interaction with GDP-bound RAN. Loss of interaction with nucleoporins and localization to the nuclear pore complex. 1 Publication1
Mutagenesisi64I → Q: No effect on homodimerization. Loss of interaction with GDP-bound RAN. Decreased interaction with nucleoporins and localization to the nuclear pore complex. 1 Publication1
Mutagenesisi66H → A: Loss of interaction with GDP-bound RAN. No effect on interaction with nucleoporins. Decreased proteins nuclear import. 1 Publication1
Mutagenesisi84M → E: Decreased homodimerization. 1 Publication1
Mutagenesisi92 – 94DED → NEN: Loss of interaction with GDP-bound RAN. No effect on interaction with nucleoporins. Loss of proteins nuclear import. 1 Publication3
Mutagenesisi102M → E: Decreased homodimerization. 1 Publication1
Mutagenesisi117D → N: Decreased interaction with GDP-bound RAN. No effect on interaction with nucleoporins. No effect on proteins nuclear import. 1 Publication1
Mutagenesisi118M → E: Loss of homodimerization. Decreased interaction with GDP-bound RAN. Decreased interaction with nucleoporins. Decreased localization to the nuclear pore complex. 1 Publication1
Mutagenesisi124Missing : Loss of interaction with GDP-bound RAN. No effect on interaction with nucleoporins. Decreased proteins nuclear import. 1 Publication1
Mutagenesisi126Missing : Decreased interaction with GDP-bound RAN. No effect on interaction with nucleoporins. No effect on proteins nuclear import. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001947771 – 127Nuclear transport factor 2Add BLAST127

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP61972
PRIDEiP61972

PTM databases

iPTMnetiP61972
PhosphoSitePlusiP61972
SwissPalmiP61972

Expressioni

Gene expression databases

BgeeiENSRNOG00000018945 Expressed in 10 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiP61972 RN

Interactioni

Subunit structurei

Homodimer (PubMed:8757804, PubMed:9533885, PubMed:11846560, PubMed:15522285). Interacts with RAN (GDP-bound form); the interaction is direct and regulates RAN nuclear import (PubMed:9822603, PubMed:8757804, PubMed:9368653, PubMed:9533885, PubMed:11846560, PubMed:15522285). Interacts with the nucleoporins NUP54, NUP58 and NUP62 (via FG repeats); recruits NUTF2 to the nuclear pore complex a step required for NUTF2-mediated GDP-bound RAN nuclear import (PubMed:8707840, PubMed:9368653, PubMed:10543952, PubMed:11846560, PubMed:15522285). Interacts with CAPG; mediates its nuclear import (By similarity).By similarity8 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi253720, 5 interactors
IntActiP61972, 1 interactor
STRINGi10116.ENSRNOP00000025608

Structurei

Secondary structure

1127
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP61972
SMRiP61972
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61972

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 121NTF2PROSITE-ProRule annotationAdd BLAST112

Phylogenomic databases

eggNOGiKOG2104 Eukaryota
ENOG4111QUX LUCA
GeneTreeiENSGT00510000047030
HOGENOMiHOG000197024
HOVERGENiHBG025070
InParanoidiP61972
OMAiQFTDFYY
OrthoDBiEOG091G0SZN
PhylomeDBiP61972
TreeFamiTF314422

Family and domain databases

CDDicd00780 NTF2, 1 hit
InterProiView protein in InterPro
IPR002075 NTF2
IPR032710 NTF2-like_dom_sf
IPR018222 Nuclear_transport_factor_2_euk
PfamiView protein in Pfam
PF02136 NTF2, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit
PROSITEiView protein in PROSITE
PS50177 NTF2_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

P61972-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA
60 70 80 90 100
AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH
110 120
QMFLLKNIND AWVCTNDMFR LALHNFG
Length:127
Mass (Da):14,478
Last modified:June 7, 2004 - v1
Checksum:i817752F20E262FD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91651 Genomic DNA Translation: CAA62839.1
BC061569 mRNA Translation: AAH61569.1
RefSeqiNP_001007630.1, NM_001007629.1
XP_006255542.1, XM_006255480.3
UniGeneiRn.107980

Genome annotation databases

EnsembliENSRNOT00000025608; ENSRNOP00000025608; ENSRNOG00000018945
GeneIDi291981
KEGGirno:291981
UCSCiRGD:1359213 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91651 Genomic DNA Translation: CAA62839.1
BC061569 mRNA Translation: AAH61569.1
RefSeqiNP_001007630.1, NM_001007629.1
XP_006255542.1, XM_006255480.3
UniGeneiRn.107980

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A2KX-ray2.50A/B1-127[»]
1AR0X-ray2.30A/B1-127[»]
1ASKX-ray2.30A/B1-127[»]
1GY6X-ray1.60A/B1-127[»]
1JB2X-ray2.00A/B1-127[»]
1JB4X-ray2.23A/B1-127[»]
1JB5X-ray2.30A/B1-127[»]
1OUNX-ray2.30A/B1-127[»]
1QMAX-ray2.50A/B/C/D2-127[»]
1U5OX-ray2.50A/B1-127[»]
5BXQX-ray2.50A/B1-127[»]
ProteinModelPortaliP61972
SMRiP61972
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi253720, 5 interactors
IntActiP61972, 1 interactor
STRINGi10116.ENSRNOP00000025608

PTM databases

iPTMnetiP61972
PhosphoSitePlusiP61972
SwissPalmiP61972

Proteomic databases

PaxDbiP61972
PRIDEiP61972

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025608; ENSRNOP00000025608; ENSRNOG00000018945
GeneIDi291981
KEGGirno:291981
UCSCiRGD:1359213 rat

Organism-specific databases

CTDi10204
RGDi1359213 Nutf2

Phylogenomic databases

eggNOGiKOG2104 Eukaryota
ENOG4111QUX LUCA
GeneTreeiENSGT00510000047030
HOGENOMiHOG000197024
HOVERGENiHBG025070
InParanoidiP61972
OMAiQFTDFYY
OrthoDBiEOG091G0SZN
PhylomeDBiP61972
TreeFamiTF314422

Miscellaneous databases

EvolutionaryTraceiP61972
PROiPR:P61972

Gene expression databases

BgeeiENSRNOG00000018945 Expressed in 10 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiP61972 RN

Family and domain databases

CDDicd00780 NTF2, 1 hit
InterProiView protein in InterPro
IPR002075 NTF2
IPR032710 NTF2-like_dom_sf
IPR018222 Nuclear_transport_factor_2_euk
PfamiView protein in Pfam
PF02136 NTF2, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit
PROSITEiView protein in PROSITE
PS50177 NTF2_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNTF2_RAT
AccessioniPrimary (citable) accession number: P61972
Secondary accession number(s): P13662
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: September 12, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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