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Protein

WD repeat-containing protein 5

Gene

Wdr5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei107Important for interaction with histone H3By similarity1
Sitei133Important for interaction with histone H3By similarity1
Sitei263Important for interaction with histone H3By similarity1
Sitei322Important for interaction with histone H3By similarity1

GO - Molecular functioni

GO - Biological processi

  • histone H3 acetylation Source: MGI
  • histone H3-K4 methylation Source: MGI
  • histone H4-K16 acetylation Source: UniProtKB
  • histone H4-K5 acetylation Source: UniProtKB
  • histone H4-K8 acetylation Source: UniProtKB
  • neuron projection development Source: MGI
  • positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • skeletal system development Source: MGI

Keywordsi

Molecular functionChromatin regulator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-3214841 PKMTs methylate histone lysines
R-MMU-3214847 HATs acetylate histones
R-MMU-3214858 RMTs methylate histone arginines
R-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-MMU-8951664 Neddylation

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein 5
Alternative name(s):
BMP2-induced 3-kb gene protein
WD repeat-containing protein BIG-3
Gene namesi
Name:Wdr5
Synonyms:Big, Big3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2155884 Wdr5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000513512 – 334WD repeat-containing protein 5Add BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki7Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki27Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei112N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP61965
MaxQBiP61965
PaxDbiP61965
PeptideAtlasiP61965
PRIDEiP61965

PTM databases

iPTMnetiP61965
PhosphoSitePlusiP61965

Expressioni

Tissue specificityi

Expressed in liver (at protein level). Detected in brain, testis and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000026917 Expressed in 279 organ(s), highest expression level in secondary oocyte
CleanExiMM_WDR5
ExpressionAtlasiP61965 baseline and differential
GenevisibleiP61965 MM

Interactioni

Subunit structurei

Interacts with PAXBP1; the interaction is direct and links a WDR5-containing histone methyltransferase complex to PAX7 and PAX3. Interacts with HCFC1. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Core component of several methyltransferase-containing complexes including MLL1/MLL, MLL2/3 (also named ASCOM complex) and MLL4/WBP7. Each complex is at least composed of ASH2L, RBBP5, WDR5, DPY30, one or more specific histone methyltransferases (KMT2A/MLL1, KMT2D/MLL2, KMT2C/MLL3 and KMT2B/MLL4), and the facultative components PAGR1, BAP18, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, MYST1/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9, TEX10 and alpha- and beta-tubulin. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts with KMT2A/MLL1 and RBBP5; the interaction is direct. Component ofthe ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. In the complex, it probably interacts directly with KAT2A, MBIP and KAT14. Interacts with histone H3. Interacts with SETD1A. Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity. Interacts with PER1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Pou5f1P202637EBI-1247084,EBI-1606219

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228326, 50 interactors
ComplexPortaliCPX-1025 GCN5-containing ATAC complex
CPX-1029 PCAF-containing ATAC complex
CPX-875 NSL histone acetyltransferase complex
CORUMiP61965
DIPiDIP-38618N
ELMiP61965
IntActiP61965, 47 interactors
MINTiP61965
STRINGi10090.ENSMUSP00000109585

Structurei

Secondary structure

1334
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP61965
SMRiP61965
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61965

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati43 – 82WD 1Add BLAST40
Repeati85 – 126WD 2Add BLAST42
Repeati128 – 168WD 3Add BLAST41
Repeati169 – 208WD 4Add BLAST40
Repeati212 – 253WD 5Add BLAST42
Repeati256 – 296WD 6Add BLAST41
Repeati299 – 333WD 7Add BLAST35

Sequence similaritiesi

Belongs to the WD repeat WDR5/wds family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0266 Eukaryota
ENOG410XP3K LUCA
GeneTreeiENSGT00810000125363
HOGENOMiHOG000091642
HOVERGENiHBG055117
InParanoidiP61965
KOiK14963
OMAiDNPICSH
OrthoDBiEOG091G0CM2
PhylomeDBiP61965
TreeFamiTF314125

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
IPR037834 WDR5
PANTHERiPTHR22847:SF560 PTHR22847:SF560, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 7 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 4 hits
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P61965-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATEEKKPET EAARAQPTPS SSATQSKPTP VKPNYALKFT LAGHTKAVSS
60 70 80 90 100
VKFSPNGEWL ASSSADKLIK IWGAYDGKFE KTISGHKLGI SDVAWSSDSN
110 120 130 140 150
LLVSASDDKT LKIWDVSSGK CLKTLKGHSN YVFCCNFNPQ SNLIVSGSFD
160 170 180 190 200
ESVRIWDVKT GKCLKTLPAH SDPVSAVHFN RDGSLIVSSS YDGLCRIWDT
210 220 230 240 250
ASGQCLKTLI DDDNPPVSFV KFSPNGKYIL AATLDNTLKL WDYSKGKCLK
260 270 280 290 300
TYTGHKNEKY CIFANFSVTG GKWIVSGSED NLVYIWNLQT KEIVQKLQGH
310 320 330
TDVVISTACH PTENIIASAA LENDKTIKLW KSDC
Length:334
Mass (Da):36,588
Last modified:June 7, 2004 - v1
Checksum:i4BF30914A2250286
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6Q3W0F6Q3W0_MOUSE
WD repeat-containing protein 5
Wdr5
281Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF416510 mRNA Translation: AAL27006.1
AK075937 mRNA Translation: BAC36067.1
BC008547 mRNA Translation: AAH08547.1
BC016103 mRNA Translation: AAH16103.1
BC025801 mRNA Translation: AAH25801.1
CCDSiCCDS15829.1
RefSeqiNP_543124.1, NM_080848.2
XP_006497735.1, XM_006497672.3
UniGeneiMm.28265

Genome annotation databases

EnsembliENSMUST00000113952; ENSMUSP00000109585; ENSMUSG00000026917
GeneIDi140858
KEGGimmu:140858
UCSCiuc012bsw.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF416510 mRNA Translation: AAL27006.1
AK075937 mRNA Translation: BAC36067.1
BC008547 mRNA Translation: AAH08547.1
BC016103 mRNA Translation: AAH16103.1
BC025801 mRNA Translation: AAH25801.1
CCDSiCCDS15829.1
RefSeqiNP_543124.1, NM_080848.2
XP_006497735.1, XM_006497672.3
UniGeneiMm.28265

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XL2X-ray2.40A/B1-334[»]
2XL3X-ray2.70A/B1-334[»]
ProteinModelPortaliP61965
SMRiP61965
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228326, 50 interactors
ComplexPortaliCPX-1025 GCN5-containing ATAC complex
CPX-1029 PCAF-containing ATAC complex
CPX-875 NSL histone acetyltransferase complex
CORUMiP61965
DIPiDIP-38618N
ELMiP61965
IntActiP61965, 47 interactors
MINTiP61965
STRINGi10090.ENSMUSP00000109585

PTM databases

iPTMnetiP61965
PhosphoSitePlusiP61965

Proteomic databases

EPDiP61965
MaxQBiP61965
PaxDbiP61965
PeptideAtlasiP61965
PRIDEiP61965

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113952; ENSMUSP00000109585; ENSMUSG00000026917
GeneIDi140858
KEGGimmu:140858
UCSCiuc012bsw.2 mouse

Organism-specific databases

CTDi11091
MGIiMGI:2155884 Wdr5

Phylogenomic databases

eggNOGiKOG0266 Eukaryota
ENOG410XP3K LUCA
GeneTreeiENSGT00810000125363
HOGENOMiHOG000091642
HOVERGENiHBG055117
InParanoidiP61965
KOiK14963
OMAiDNPICSH
OrthoDBiEOG091G0CM2
PhylomeDBiP61965
TreeFamiTF314125

Enzyme and pathway databases

ReactomeiR-MMU-3214841 PKMTs methylate histone lysines
R-MMU-3214847 HATs acetylate histones
R-MMU-3214858 RMTs methylate histone arginines
R-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-MMU-8951664 Neddylation

Miscellaneous databases

EvolutionaryTraceiP61965
PROiPR:P61965
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026917 Expressed in 279 organ(s), highest expression level in secondary oocyte
CleanExiMM_WDR5
ExpressionAtlasiP61965 baseline and differential
GenevisibleiP61965 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
IPR037834 WDR5
PANTHERiPTHR22847:SF560 PTHR22847:SF560, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 7 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 4 hits
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWDR5_MOUSE
AccessioniPrimary (citable) accession number: P61965
Secondary accession number(s): Q91VA5
, Q922C9, Q9NWX7, Q9UGP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 7, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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