UniProtKB - P61965 (WDR5_MOUSE)
WD repeat-containing protein 5
Wdr5
Functioni
Contributes to histone modification (By similarity).
May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4' (By similarity).
As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (By similarity).
H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (By similarity).
As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (By similarity).
May regulate osteoblasts differentiation (PubMed:11551928).
In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (By similarity).
By similarity1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 107 | Important for interaction with histone H3By similarity | 1 | |
Sitei | 133 | Important for interaction with histone H3By similarity | 1 | |
Sitei | 263 | Important for interaction with histone H3By similarity | 1 | |
Sitei | 322 | Important for interaction with histone H3By similarity | 1 |
GO - Molecular functioni
- histone binding Source: GO_Central
- histone methyltransferase activity (H3-K4 specific) Source: Ensembl
- methylated histone binding Source: UniProtKB
GO - Biological processi
- chromatin organization Source: UniProtKB-KW
- histone H3 acetylation Source: MGI
- histone H3-K14 acetylation Source: Ensembl
- histone H3-K4 methylation Source: MGI
- histone H4-K16 acetylation Source: UniProtKB
- histone H4-K5 acetylation Source: UniProtKB
- histone H4-K8 acetylation Source: UniProtKB
- negative regulation of histone H3-K4 methylation Source: MGI
- neuron projection development Source: MGI
- positive regulation of gluconeogenesis Source: MGI
- positive regulation of histone H3-K4 methylation Source: MGI
- regulation of cell cycle Source: ComplexPortal
- regulation of cell division Source: ComplexPortal
- regulation of dosage compensation by inactivation of X chromosome Source: ComplexPortal
- regulation of embryonic development Source: ComplexPortal
- regulation of histone deacetylation Source: Ensembl
- regulation of transcription by RNA polymerase II Source: Ensembl
- regulation of tubulin deacetylation Source: Ensembl
- skeletal system development Source: MGI
Keywordsi
Molecular function | Chromatin regulator |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-3214841, PKMTs methylate histone lysines R-MMU-3214847, HATs acetylate histones R-MMU-3214858, RMTs methylate histone arginines R-MMU-8936459, RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-MMU-8951664, Neddylation |
Names & Taxonomyi
Protein namesi | Recommended name: WD repeat-containing protein 5Alternative name(s): BMP2-induced 3-kb gene protein WD repeat-containing protein BIG-3 |
Gene namesi | Name:Wdr5 Synonyms:Big, Big3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2155884, Wdr5 |
VEuPathDBi | HostDB:ENSMUSG00000026917 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- ATAC complex Source: Ensembl
- histone acetyltransferase complex Source: UniProtKB
- histone methyltransferase complex Source: UniProtKB
- MLL1 complex Source: UniProtKB
- MLL3/4 complex Source: MGI
- NSL complex Source: Ensembl
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
- Set1C/COMPASS complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000051351 | 2 – 334 | WD repeat-containing protein 5Add BLAST | 333 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Cross-linki | 7 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 27 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 46 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 112 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Ubl conjugationProteomic databases
EPDi | P61965 |
MaxQBi | P61965 |
PaxDbi | P61965 |
PeptideAtlasi | P61965 |
PRIDEi | P61965 |
ProteomicsDBi | 299669 |
PTM databases
iPTMneti | P61965 |
PhosphoSitePlusi | P61965 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000026917, Expressed in secondary oocyte and 303 other tissues |
ExpressionAtlasi | P61965, baseline and differential |
Genevisiblei | P61965, MM |
Interactioni
Subunit structurei
Interacts with PAXBP1; the interaction is direct and links a WDR5-containing histone methyltransferase complex to PAX7 and PAX3 (PubMed:22862948).
Interacts with HCFC1 (By similarity).
Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4 (By similarity).
Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30 (By similarity). Core component of several methyltransferase-containing complexes including MLL1/MLL, MLL2/3 (also named ASCOM complex) and MLL4/WBP7 (PubMed:21335234). Each complex is at least composed of ASH2L, RBBP5, WDR5, DPY30, one or more specific histone methyltransferases (KMT2A/MLL1, KMT2D/MLL2, KMT2C/MLL3 and KMT2B/MLL4), and the facultative components PAGR1, BAP18, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, MYST1/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9, TEX10 and alpha- and beta-tubulin (PubMed:21335234).
Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1 (By similarity).
Interacts with KMT2A/MLL1 (via WIN motif) and RBBP5; the interaction is direct (By similarity).
Component ofthe ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1 (By similarity). In the complex, it probably interacts directly with KAT2A, MBIP and KAT14 (By similarity).
Interacts with histone H3 (By similarity).
Interacts with SETD1A (via WIN motif) (By similarity).
Component of a histone methylation complex composed of at least ZNF335, RBBP5, ASH2L and WDR5; the complex may have histone H3-specific methyltransferase activity, however does not have specificity for 'Lys-4' of histone H3 (By similarity).
Interacts with ZNF335 (PubMed:23178126). Components of this complex may associate with components of the ZNF335-CCAR2-EMSY nuclear receptor-mediated transcription complex to form a complex at least composed of ZNF335, HCFC1, CCAR2, EMSY, MKI67, RBBP5, ASH2L and WDR5 (By similarity).
Interacts with PER1 (By similarity).
Interacts with KMT2D (via WIN motif) (PubMed:21335234).
Interacts with KMT2B (via WIN motif), KMT2C (via WIN motif) and SETD1B (via WIN motif) (By similarity).
By similarity3 PublicationsBinary interactionsi
P61965
With | #Exp. | IntAct |
---|---|---|
Pou5f1 [P20263] | 7 | EBI-1247084,EBI-1606219 |
GO - Molecular functioni
- histone binding Source: GO_Central
- methylated histone binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 228326, 62 interactors |
ComplexPortali | CPX-1025, GCN5-containing ATAC complex CPX-1029, PCAF-containing ATAC complex CPX-875, NSL histone acetyltransferase complex |
CORUMi | P61965 |
DIPi | DIP-38618N |
ELMi | P61965 |
IntActi | P61965, 46 interactors |
MINTi | P61965 |
STRINGi | 10090.ENSMUSP00000109585 |
Miscellaneous databases
RNActi | P61965, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P61965 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P61965 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 43 – 82 | WD 1Add BLAST | 40 | |
Repeati | 85 – 126 | WD 2Add BLAST | 42 | |
Repeati | 128 – 168 | WD 3Add BLAST | 41 | |
Repeati | 169 – 208 | WD 4Add BLAST | 40 | |
Repeati | 212 – 253 | WD 5Add BLAST | 42 | |
Repeati | 256 – 296 | WD 6Add BLAST | 41 | |
Repeati | 299 – 333 | WD 7Add BLAST | 35 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 31 | DisorderedSequence analysisAdd BLAST | 31 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 14 – 31 | Polar residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0266, Eukaryota |
GeneTreei | ENSGT00940000154143 |
InParanoidi | P61965 |
OMAi | EKTIAGH |
OrthoDBi | 957291at2759 |
PhylomeDBi | P61965 |
TreeFami | TF314125 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
IDEALi | IID50144 |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 7 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 4 hits PS50082, WD_REPEATS_2, 6 hits PS50294, WD_REPEATS_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MATEEKKPET EAARAQPTPS SSATQSKPTP VKPNYALKFT LAGHTKAVSS
60 70 80 90 100
VKFSPNGEWL ASSSADKLIK IWGAYDGKFE KTISGHKLGI SDVAWSSDSN
110 120 130 140 150
LLVSASDDKT LKIWDVSSGK CLKTLKGHSN YVFCCNFNPQ SNLIVSGSFD
160 170 180 190 200
ESVRIWDVKT GKCLKTLPAH SDPVSAVHFN RDGSLIVSSS YDGLCRIWDT
210 220 230 240 250
ASGQCLKTLI DDDNPPVSFV KFSPNGKYIL AATLDNTLKL WDYSKGKCLK
260 270 280 290 300
TYTGHKNEKY CIFANFSVTG GKWIVSGSED NLVYIWNLQT KEIVQKLQGH
310 320 330
TDVVISTACH PTENIIASAA LENDKTIKLW KSDC
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF6Q3W0 | F6Q3W0_MOUSE | WD repeat-containing protein 5 | Wdr5 | 281 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF416510 mRNA Translation: AAL27006.1 AK075937 mRNA Translation: BAC36067.1 BC008547 mRNA Translation: AAH08547.1 BC016103 mRNA Translation: AAH16103.1 BC025801 mRNA Translation: AAH25801.1 |
CCDSi | CCDS15829.1 |
RefSeqi | NP_543124.1, NM_080848.2 XP_006497735.1, XM_006497672.3 |
Genome annotation databases
Ensembli | ENSMUST00000113952; ENSMUSP00000109585; ENSMUSG00000026917 |
GeneIDi | 140858 |
KEGGi | mmu:140858 |
UCSCi | uc012bsw.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF416510 mRNA Translation: AAL27006.1 AK075937 mRNA Translation: BAC36067.1 BC008547 mRNA Translation: AAH08547.1 BC016103 mRNA Translation: AAH16103.1 BC025801 mRNA Translation: AAH25801.1 |
CCDSi | CCDS15829.1 |
RefSeqi | NP_543124.1, NM_080848.2 XP_006497735.1, XM_006497672.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2XL2 | X-ray | 2.40 | A/B | 1-334 | [»] | |
2XL3 | X-ray | 2.70 | A/B | 1-334 | [»] | |
SMRi | P61965 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 228326, 62 interactors |
ComplexPortali | CPX-1025, GCN5-containing ATAC complex CPX-1029, PCAF-containing ATAC complex CPX-875, NSL histone acetyltransferase complex |
CORUMi | P61965 |
DIPi | DIP-38618N |
ELMi | P61965 |
IntActi | P61965, 46 interactors |
MINTi | P61965 |
STRINGi | 10090.ENSMUSP00000109585 |
PTM databases
iPTMneti | P61965 |
PhosphoSitePlusi | P61965 |
Proteomic databases
EPDi | P61965 |
MaxQBi | P61965 |
PaxDbi | P61965 |
PeptideAtlasi | P61965 |
PRIDEi | P61965 |
ProteomicsDBi | 299669 |
Protocols and materials databases
Antibodypediai | 31978, 430 antibodies from 42 providers |
DNASUi | 140858 |
Genome annotation databases
Ensembli | ENSMUST00000113952; ENSMUSP00000109585; ENSMUSG00000026917 |
GeneIDi | 140858 |
KEGGi | mmu:140858 |
UCSCi | uc012bsw.2, mouse |
Organism-specific databases
CTDi | 11091 |
MGIi | MGI:2155884, Wdr5 |
VEuPathDBi | HostDB:ENSMUSG00000026917 |
Phylogenomic databases
eggNOGi | KOG0266, Eukaryota |
GeneTreei | ENSGT00940000154143 |
InParanoidi | P61965 |
OMAi | EKTIAGH |
OrthoDBi | 957291at2759 |
PhylomeDBi | P61965 |
TreeFami | TF314125 |
Enzyme and pathway databases
Reactomei | R-MMU-3214841, PKMTs methylate histone lysines R-MMU-3214847, HATs acetylate histones R-MMU-3214858, RMTs methylate histone arginines R-MMU-8936459, RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-MMU-8951664, Neddylation |
Miscellaneous databases
BioGRID-ORCSi | 140858, 27 hits in 62 CRISPR screens |
ChiTaRSi | Wdr5, mouse |
EvolutionaryTracei | P61965 |
PROi | PR:P61965 |
RNActi | P61965, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026917, Expressed in secondary oocyte and 303 other tissues |
ExpressionAtlasi | P61965, baseline and differential |
Genevisiblei | P61965, MM |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
IDEALi | IID50144 |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 7 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 4 hits PS50082, WD_REPEATS_2, 6 hits PS50294, WD_REPEATS_REGION, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | WDR5_MOUSE | |
Accessioni | P61965Primary (citable) accession number: P61965 Secondary accession number(s): Q91VA5 Q9UGP9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2004 |
Last sequence update: | June 7, 2004 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families