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Protein

4-aminobutyrate aminotransferase, mitochondrial

Gene

Abat

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine (By similarity).By similarity

Catalytic activityi

4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.
(S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate.

Cofactori

pyridoxal 5'-phosphateBy similarity, [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per homodimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi163Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity1
Metal bindingi166Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity1
Binding sitei220SubstrateBy similarity1
Binding sitei381Pyridoxal phosphate; shared with dimeric partnerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processNeurotransmitter degradation
LigandIron, Iron-sulfur, Metal-binding, Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-916853 Degradation of GABA

Names & Taxonomyi

Protein namesi
Recommended name:
4-aminobutyrate aminotransferase, mitochondrial (EC:2.6.1.19)
Alternative name(s):
(S)-3-amino-2-methylpropionate transaminase (EC:2.6.1.22)
GABA aminotransferase
Short name:
GABA-AT
Gamma-amino-N-butyrate transaminase
Short name:
GABA transaminase
Short name:
GABA-T
L-AIBAT
Gene namesi
Name:Abat
Synonyms:Gabat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2443582 Abat

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28MitochondrionBy similarityAdd BLAST28
ChainiPRO_000000125029 – 5004-aminobutyrate aminotransferase, mitochondrialAdd BLAST472

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei231N6-succinyllysineCombined sources1
Modified residuei252N6-acetyllysine; alternateCombined sources1
Modified residuei252N6-succinyllysine; alternateCombined sources1
Modified residuei279N6-acetyllysineCombined sources1
Modified residuei318N6-acetyllysineCombined sources1
Disulfide bondi321InterchainBy similarity
Modified residuei357N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei413N6-acetyllysine; alternateCombined sources1
Modified residuei413N6-succinyllysine; alternateCombined sources1
Modified residuei452N6-acetyllysineCombined sources1
Modified residuei470N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

MaxQBiP61922
PaxDbiP61922
PeptideAtlasiP61922
PRIDEiP61922

2D gel databases

REPRODUCTION-2DPAGEiIPI00407499

PTM databases

iPTMnetiP61922
PhosphoSitePlusiP61922
SwissPalmiP61922

Expressioni

Gene expression databases

BgeeiENSMUSG00000057880 Expressed in 281 organ(s), highest expression level in spinal cord
CleanExiMM_ABAT
ExpressionAtlasiP61922 baseline and differential
GenevisibleiP61922 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP61922, 2 interactors
MINTiP61922
STRINGi10090.ENSMUSP00000063548

Structurei

3D structure databases

ProteinModelPortaliP61922
SMRiP61922
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 165Pyridoxal phosphate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1405 Eukaryota
COG0160 LUCA
GeneTreeiENSGT00550000074885
HOGENOMiHOG000020208
HOVERGENiHBG000634
InParanoidiP61922
KOiK13524
OMAiIQTGGCG
OrthoDBiEOG091G08T5
PhylomeDBiP61922
TreeFamiTF105021

Family and domain databases

CDDicd00610 OAT_like, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
InterProiView protein in InterPro
IPR004631 4NH2But_aminotransferase_euk
IPR005814 Aminotrans_3
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00202 Aminotran_3, 1 hit
PIRSFiPIRSF000521 Transaminase_4ab_Lys_Orn, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR00699 GABAtrns_euk, 1 hit
PROSITEiView protein in PROSITE
PS00600 AA_TRANSFER_CLASS_3, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P61922-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAFLLITRRL ACSSQKNLHL FIPGSRYISQ AAAKVDIEFD YDGPLMKTEV
60 70 80 90 100
PGPRSKELMK QLNTIQNAEA VHFFCNYEES RGNYLVDVDG NRMLDLYSQI
110 120 130 140 150
SSVPIGYNHP ALAKLVQQPQ NASTFINRPA LGILPPENFV DKLQESLMSV
160 170 180 190 200
APRGMSQLIT MACGSCSNEN AFKTIFMWYR SKERGQRGFS KEELETCMVN
210 220 230 240 250
QSPGCPDYSI LSFMGAFHGR TMGCLATTHS KAIHKIDIPS FDWPIAPFPR
260 270 280 290 300
LKYPLEEFTT DNQQEEARCL EEVEDLIVKY RKKKRTVAGI IVEPIQSEGG
310 320 330 340 350
DNHASDDFFR KLRDIARKHG CAFLVDEVQT GGGCTGKFWA HEHWGLDDPA
360 370 380 390 400
DVMTFSKKMM TGGFFHKEEF RPSAPYRIFN TWLGDPSKNL LLAEVINIIK
410 420 430 440 450
REDLLNNVAR VGKTLLTGLL DLQAQYPQFI SRVRGRGTFC SFDTPDEAIR
460 470 480 490 500
NKLILIARNK GVVLGGCGDK SIRFRPTLVF RDHHAHLFLS IFSGILADFK
Note: No experimental confirmation available.
Length:500
Mass (Da):56,452
Last modified:June 7, 2004 - v1
Checksum:i85AA331AB48355F3
GO
Isoform 2 (identifier: P61922-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     319-374: Missing.

Note: No experimental confirmation available.
Show »
Length:444
Mass (Da):50,237
Checksum:i1E7C6A3DFDDD639D
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F7C9G3F7C9G3_MOUSE
4-aminobutyrate aminotransferase, m...
Abat
232Annotation score:
Q3TUE8Q3TUE8_MOUSE
4-aminobutyrate aminotransferase, m...
Abat
204Annotation score:
F6RN86F6RN86_MOUSE
4-aminobutyrate aminotransferase, m...
Abat
109Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012005319 – 374Missing in isoform 2. 1 PublicationAdd BLAST56

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC058079 mRNA Translation: AAH58079.1
BC058521 mRNA Translation: AAH58521.1
AK036128 mRNA Translation: BAC29312.1
CCDSiCCDS27939.1 [P61922-1]
CCDS49754.1 [P61922-2]
RefSeqiNP_001164449.1, NM_001170978.1 [P61922-2]
NP_766549.2, NM_172961.3 [P61922-1]
UniGeneiMm.259315

Genome annotation databases

EnsembliENSMUST00000065987; ENSMUSP00000063548; ENSMUSG00000057880 [P61922-1]
ENSMUST00000115839; ENSMUSP00000111505; ENSMUSG00000057880 [P61922-2]
GeneIDi268860
KEGGimmu:268860
UCSCiuc007yco.2 mouse [P61922-1]
uc007ycp.2 mouse [P61922-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC058079 mRNA Translation: AAH58079.1
BC058521 mRNA Translation: AAH58521.1
AK036128 mRNA Translation: BAC29312.1
CCDSiCCDS27939.1 [P61922-1]
CCDS49754.1 [P61922-2]
RefSeqiNP_001164449.1, NM_001170978.1 [P61922-2]
NP_766549.2, NM_172961.3 [P61922-1]
UniGeneiMm.259315

3D structure databases

ProteinModelPortaliP61922
SMRiP61922
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP61922, 2 interactors
MINTiP61922
STRINGi10090.ENSMUSP00000063548

PTM databases

iPTMnetiP61922
PhosphoSitePlusiP61922
SwissPalmiP61922

2D gel databases

REPRODUCTION-2DPAGEiIPI00407499

Proteomic databases

MaxQBiP61922
PaxDbiP61922
PeptideAtlasiP61922
PRIDEiP61922

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065987; ENSMUSP00000063548; ENSMUSG00000057880 [P61922-1]
ENSMUST00000115839; ENSMUSP00000111505; ENSMUSG00000057880 [P61922-2]
GeneIDi268860
KEGGimmu:268860
UCSCiuc007yco.2 mouse [P61922-1]
uc007ycp.2 mouse [P61922-2]

Organism-specific databases

CTDi18
MGIiMGI:2443582 Abat

Phylogenomic databases

eggNOGiKOG1405 Eukaryota
COG0160 LUCA
GeneTreeiENSGT00550000074885
HOGENOMiHOG000020208
HOVERGENiHBG000634
InParanoidiP61922
KOiK13524
OMAiIQTGGCG
OrthoDBiEOG091G08T5
PhylomeDBiP61922
TreeFamiTF105021

Enzyme and pathway databases

ReactomeiR-MMU-916853 Degradation of GABA

Miscellaneous databases

ChiTaRSiAbat mouse
PROiPR:P61922
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057880 Expressed in 281 organ(s), highest expression level in spinal cord
CleanExiMM_ABAT
ExpressionAtlasiP61922 baseline and differential
GenevisibleiP61922 MM

Family and domain databases

CDDicd00610 OAT_like, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
InterProiView protein in InterPro
IPR004631 4NH2But_aminotransferase_euk
IPR005814 Aminotrans_3
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00202 Aminotran_3, 1 hit
PIRSFiPIRSF000521 Transaminase_4ab_Lys_Orn, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR00699 GABAtrns_euk, 1 hit
PROSITEiView protein in PROSITE
PS00600 AA_TRANSFER_CLASS_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGABT_MOUSE
AccessioniPrimary (citable) accession number: P61922
Secondary accession number(s): Q8BZA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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