UniProtKB - P61875 (DPOL_PYRFU)
Protein
DNA polymerase
Gene
pol
Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Functioni
In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.1 Publication
Catalytic activityi
- EC:2.7.7.7
Activity regulationi
An 11-mer corresponding to the PIP-box of RfcL inhibits DNA synthesis.1 Publication
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: UniProtKB-KW
- endonuclease activity Source: UniProtKB-KW
- nucleotide binding Source: InterPro
GO - Biological processi
- DNA replication Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase |
Biological process | DNA replication |
Enzyme and pathway databases
BioCyci | PFUR186497:G1FZR-220-MONOMER |
BRENDAi | 2.7.7.7, 5243 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pol Ordered Locus Names:PF0212 |
Organismi | Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) |
Taxonomic identifieri | 186497 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › |
Proteomesi |
|
Pathology & Biotechi
Biotechnological usei
Because Pfu DNA polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, it is routinely used for PCR. It is sold by a number of companies including Promega, Stratagene and ThermoScientific. Various mutations to improve its abilities have been incorporated.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046480 | 1 – 775 | DNA polymeraseAdd BLAST | 775 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
STRINGi | 186497.PF0212 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P61875 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P61875 |
Family & Domainsi
Sequence similaritiesi
Belongs to the DNA polymerase type-B family.Curated
Phylogenomic databases
eggNOGi | arCOG00328, Archaea |
HOGENOMi | CLU_000203_6_0_2 |
OMAi | GQQVKFI |
OrthoDBi | 35869at2157 |
Family and domain databases
Gene3Di | 1.10.132.60, 1 hit 3.30.420.10, 1 hit 3.90.1600.10, 1 hit |
IDEALi | IID90009 |
InterProi | View protein in InterPro IPR006172, DNA-dir_DNA_pol_B IPR017964, DNA-dir_DNA_pol_B_CS IPR006133, DNA-dir_DNA_pol_B_exonuc IPR006134, DNA-dir_DNA_pol_B_multi_dom IPR043502, DNA/RNA_pol_sf IPR042087, DNA_pol_B_C IPR023211, DNA_pol_palm_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF00136, DNA_pol_B, 1 hit PF03104, DNA_pol_B_exo1, 2 hits |
PRINTSi | PR00106, DNAPOLB |
SMARTi | View protein in SMART SM00486, POLBc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00116, DNA_POLYMERASE_B, 1 hit |
i Sequence
Sequence statusi: Complete.
P61875-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MILDVDYITE EGKPVIRLFK KENGKFKIEH DRTFRPYIYA LLRDDSKIEE
60 70 80 90 100
VKKITGERHG KIVRIVDVEK VEKKFLGKPI TVWKLYLEHP QDVPTIREKV
110 120 130 140 150
REHPAVVDIF EYDIPFAKRY LIDKGLIPME GEEELKILAF DIETLYHEGE
160 170 180 190 200
EFGKGPIIMI SYADENEAKV ITWKNIDLPY VEVVSSEREM IKRFLRIIRE
210 220 230 240 250
KDPDIIVTYN GDSFDFPYLA KRAEKLGIKL TIGRDGSEPK MQRIGDMTAV
260 270 280 290 300
EVKGRIHFDL YHVITRTINL PTYTLEAVYE AIFGKPKEKV YADEIAKAWE
310 320 330 340 350
SGENLERVAK YSMEDAKATY ELGKEFLPME IQLSRLVGQP LWDVSRSSTG
360 370 380 390 400
NLVEWFLLRK AYERNEVAPN KPSEEEYQRR LRESYTGGFV KEPEKGLWEN
410 420 430 440 450
IVYLDFRALY PSIIITHNVS PDTLNLEGCK NYDIAPQVGH KFCKDIPGFI
460 470 480 490 500
PSLLGHLLEE RQKIKTKMKE TQDPIEKILL DYRQKAIKLL ANSFYGYYGY
510 520 530 540 550
AKARWYCKEC AESVTAWGRK YIELVWKELE EKFGFKVLYI DTDGLYATIP
560 570 580 590 600
GGESEEIKKK ALEFVKYINS KLPGLLELEY EGFYKRGFFV TKKRYAVIDE
610 620 630 640 650
EGKVITRGLE IVRRDWSEIA KETQARVLET ILKHGDVEEA VRIVKEVIQK
660 670 680 690 700
LANYEIPPEK LAIYEQITRP LHEYKAIGPH VAVAKKLAAK GVKIKPGMVI
710 720 730 740 750
GYIVLRGDGP ISNRAILAEE YDPKKHKYDA EYYIENQVLP AVLRILEGFG
760 770
YRKEDLRYQK TRQVGLTSWL NIKKS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D12983 Genomic DNA Translation: BAA02362.1 AE009950 Genomic DNA Translation: AAL80336.1 |
PIRi | S35543 |
RefSeqi | WP_011011325.1, NC_018092.1 |
Genome annotation databases
EnsemblBacteriai | AAL80336; AAL80336; PF0212 |
GeneIDi | 41712003 |
KEGGi | pfu:PF0212 |
PATRICi | fig|186497.12.peg.220 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D12983 Genomic DNA Translation: BAA02362.1 AE009950 Genomic DNA Translation: AAL80336.1 |
PIRi | S35543 |
RefSeqi | WP_011011325.1, NC_018092.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JGU | X-ray | 2.60 | A | 1-775 | [»] | |
3A2F | X-ray | 2.67 | A | 1-775 | [»] | |
4AHC | X-ray | 2.40 | A/B | 1-775 | [»] | |
4AIL | X-ray | 2.90 | C | 1-775 | [»] | |
SMRi | P61875 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 186497.PF0212 |
Genome annotation databases
EnsemblBacteriai | AAL80336; AAL80336; PF0212 |
GeneIDi | 41712003 |
KEGGi | pfu:PF0212 |
PATRICi | fig|186497.12.peg.220 |
Phylogenomic databases
eggNOGi | arCOG00328, Archaea |
HOGENOMi | CLU_000203_6_0_2 |
OMAi | GQQVKFI |
OrthoDBi | 35869at2157 |
Enzyme and pathway databases
BioCyci | PFUR186497:G1FZR-220-MONOMER |
BRENDAi | 2.7.7.7, 5243 |
Miscellaneous databases
EvolutionaryTracei | P61875 |
Family and domain databases
Gene3Di | 1.10.132.60, 1 hit 3.30.420.10, 1 hit 3.90.1600.10, 1 hit |
IDEALi | IID90009 |
InterProi | View protein in InterPro IPR006172, DNA-dir_DNA_pol_B IPR017964, DNA-dir_DNA_pol_B_CS IPR006133, DNA-dir_DNA_pol_B_exonuc IPR006134, DNA-dir_DNA_pol_B_multi_dom IPR043502, DNA/RNA_pol_sf IPR042087, DNA_pol_B_C IPR023211, DNA_pol_palm_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF00136, DNA_pol_B, 1 hit PF03104, DNA_pol_B_exo1, 2 hits |
PRINTSi | PR00106, DNAPOLB |
SMARTi | View protein in SMART SM00486, POLBc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00116, DNA_POLYMERASE_B, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOL_PYRFU | |
Accessioni | P61875Primary (citable) accession number: P61875 Secondary accession number(s): P80061, P95584 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2004 |
Last sequence update: | June 7, 2004 | |
Last modified: | February 10, 2021 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families