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Protein

DNA polymerase

Gene

pol

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Activity regulationi

An 11-mer corresponding to the PIP-box of RfcL inhibits DNA synthesis.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase
Biological processDNA replication

Enzyme and pathway databases

BRENDAi2.7.7.7 5243

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase (EC:2.7.7.7)
Alternative name(s):
DNA polymerase B
Pfu polymerase
Pol I
Gene namesi
Name:pol
Ordered Locus Names:PF0212
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Pathology & Biotechi

Biotechnological usei

Because Pfu DNA polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, it is routinely used for PCR. It is sold by a number of companies including Promega, Stratagene and ThermoScientific. Various mutations to improve its abilities have been incorporated.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464801 – 775DNA polymeraseAdd BLAST775

Proteomic databases

PRIDEiP61875

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi186497.PF0212

Structurei

Secondary structure

1775
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP61875
SMRiP61875
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61875

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Phylogenomic databases

eggNOGiarCOG00328 Archaea
COG0417 LUCA
HOGENOMiHOG000222650
KOiK02319
OMAiCLVNYTE
OrthoDBiPOG093Z00EA

Family and domain databases

Gene3Di3.30.420.10, 1 hit
3.90.1600.10, 1 hit
InterProiView protein in InterPro
IPR006172 DNA-dir_DNA_pol_B
IPR017964 DNA-dir_DNA_pol_B_CS
IPR006133 DNA-dir_DNA_pol_B_exonuc
IPR006134 DNA-dir_DNA_pol_B_multi_dom
IPR023211 DNA_pol_palm_dom_sf
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF00136 DNA_pol_B, 1 hit
PF03104 DNA_pol_B_exo1, 2 hits
PRINTSiPR00106 DNAPOLB
SMARTiView protein in SMART
SM00486 POLBc, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
PROSITEiView protein in PROSITE
PS00116 DNA_POLYMERASE_B, 1 hit

Sequencei

Sequence statusi: Complete.

P61875-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MILDVDYITE EGKPVIRLFK KENGKFKIEH DRTFRPYIYA LLRDDSKIEE
60 70 80 90 100
VKKITGERHG KIVRIVDVEK VEKKFLGKPI TVWKLYLEHP QDVPTIREKV
110 120 130 140 150
REHPAVVDIF EYDIPFAKRY LIDKGLIPME GEEELKILAF DIETLYHEGE
160 170 180 190 200
EFGKGPIIMI SYADENEAKV ITWKNIDLPY VEVVSSEREM IKRFLRIIRE
210 220 230 240 250
KDPDIIVTYN GDSFDFPYLA KRAEKLGIKL TIGRDGSEPK MQRIGDMTAV
260 270 280 290 300
EVKGRIHFDL YHVITRTINL PTYTLEAVYE AIFGKPKEKV YADEIAKAWE
310 320 330 340 350
SGENLERVAK YSMEDAKATY ELGKEFLPME IQLSRLVGQP LWDVSRSSTG
360 370 380 390 400
NLVEWFLLRK AYERNEVAPN KPSEEEYQRR LRESYTGGFV KEPEKGLWEN
410 420 430 440 450
IVYLDFRALY PSIIITHNVS PDTLNLEGCK NYDIAPQVGH KFCKDIPGFI
460 470 480 490 500
PSLLGHLLEE RQKIKTKMKE TQDPIEKILL DYRQKAIKLL ANSFYGYYGY
510 520 530 540 550
AKARWYCKEC AESVTAWGRK YIELVWKELE EKFGFKVLYI DTDGLYATIP
560 570 580 590 600
GGESEEIKKK ALEFVKYINS KLPGLLELEY EGFYKRGFFV TKKRYAVIDE
610 620 630 640 650
EGKVITRGLE IVRRDWSEIA KETQARVLET ILKHGDVEEA VRIVKEVIQK
660 670 680 690 700
LANYEIPPEK LAIYEQITRP LHEYKAIGPH VAVAKKLAAK GVKIKPGMVI
710 720 730 740 750
GYIVLRGDGP ISNRAILAEE YDPKKHKYDA EYYIENQVLP AVLRILEGFG
760 770
YRKEDLRYQK TRQVGLTSWL NIKKS
Length:775
Mass (Da):90,113
Last modified:June 7, 2004 - v1
Checksum:i5668E3D9F4DBD40E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12983 Genomic DNA Translation: BAA02362.1
AE009950 Genomic DNA Translation: AAL80336.1
PIRiS35543
RefSeqiWP_011011325.1, NC_003413.1

Genome annotation databases

EnsemblBacteriaiAAL80336; AAL80336; PF0212
GeneIDi1468044
KEGGipfu:PF0212
PATRICifig|186497.12.peg.220

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12983 Genomic DNA Translation: BAA02362.1
AE009950 Genomic DNA Translation: AAL80336.1
PIRiS35543
RefSeqiWP_011011325.1, NC_003413.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JGUX-ray2.60A1-775[»]
3A2FX-ray2.67A1-775[»]
4AHCX-ray2.40A/B1-775[»]
4AILX-ray2.90C1-775[»]
ProteinModelPortaliP61875
SMRiP61875
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0212

Proteomic databases

PRIDEiP61875

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80336; AAL80336; PF0212
GeneIDi1468044
KEGGipfu:PF0212
PATRICifig|186497.12.peg.220

Phylogenomic databases

eggNOGiarCOG00328 Archaea
COG0417 LUCA
HOGENOMiHOG000222650
KOiK02319
OMAiCLVNYTE
OrthoDBiPOG093Z00EA

Enzyme and pathway databases

BRENDAi2.7.7.7 5243

Miscellaneous databases

EvolutionaryTraceiP61875

Family and domain databases

Gene3Di3.30.420.10, 1 hit
3.90.1600.10, 1 hit
InterProiView protein in InterPro
IPR006172 DNA-dir_DNA_pol_B
IPR017964 DNA-dir_DNA_pol_B_CS
IPR006133 DNA-dir_DNA_pol_B_exonuc
IPR006134 DNA-dir_DNA_pol_B_multi_dom
IPR023211 DNA_pol_palm_dom_sf
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF00136 DNA_pol_B, 1 hit
PF03104 DNA_pol_B_exo1, 2 hits
PRINTSiPR00106 DNAPOLB
SMARTiView protein in SMART
SM00486 POLBc, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
PROSITEiView protein in PROSITE
PS00116 DNA_POLYMERASE_B, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPOL_PYRFU
AccessioniPrimary (citable) accession number: P61875
Secondary accession number(s): P80061, P95584
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 7, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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